Phaseolin nucleotide sequence diversity in Phaseolus. I. Intraspecific diversity in Phaseolus vulgaris.

Abstract:

:Most information about the molecular biology of phaseolin, the major seed storage protein in Phaseolus vulgaris, has been obtained from the T-type phaseolin, which is characteristic of the Andean gene pool of the species. In the work reported here, two cDNA clones for the S-type phaseolin representing the other major, Middle American gene pool were isolated and sequenced. Analysis of the DNA sequences revealed the presence of two subtypes of S phaseolin, alpha and beta, depending on the presence or absence, respectively, of a 27-bp direct repeat. These are similar to the alpha- and beta-phaseolin subtypes found in the Andean, T phaseolin; however, the additional 15-bp direct repeat also found in the T alpha-phaseolin gene type was apparently absent from the S alpha-phaseolin genes. The overall sequence identity was greater between the alpha or beta subtypes of different gene pools than between the alpha or beta subtypes within gene pools. This implies that the gene subtypes were formed prior to the formation of the two major gene pools of P. vulgaris. Analysis of the putative amino acid sequence revealed that both the 'Sanilac' phaseolin subtypes contained an additional methionine, however, not at the same site. This opens the possibility of increasing the nutritionally limiting methionine level in phaseolin either through protein engineering or by screening accessions for recombinant phaseolin sequences that combine both substitutions.

journal_name

Genome

journal_title

Genome

authors

Kami JA,Gepts P

doi

10.1139/g94-107

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

751-7

issue

5

eissn

0831-2796

issn

1480-3321

journal_volume

37

pub_type

杂志文章

相关文献

GENOME文献大全
  • Isochromosome Y in an infertile heifer.

    abstract::The chromosome makeup of a 3-year-old crossbred heifer was examined in an attempt to explore the cytogenetic causes of her infertility. The heifer proved to be a mosaic carrying predominantly XY cells and with approximately 30% of cells lacking a normal Y. The minor population included cells carrying a dicentric isoch...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g90-104

    authors: Pinheiro LE,Mikich AB,Bechara GH,Almeida IL,Basrur PK

    更新日期:1990-10-01 00:00:00

  • Arrays of repetitive DNA elements in the largest chromosomes of Toxoplasma gondii.

    abstract::A novel tandemly repeated DNA structure of Toxoplasma gondii that meets the requirements assigned for satellital DNA was characterized. A DNA fragment of 1002 bp contains two different elements of repetitive DNA families named ABGTg7 and ABGTg8.2. Both repeats are members of a more complex tandem structure where ABGTg...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g98-120

    authors: Matrajt M,Angel SO,Pszenny V,Guarnera E,Roos DS,Garberi JC

    更新日期:1999-04-01 00:00:00

  • Authentication of Ginkgo biloba herbal dietary supplements using DNA barcoding.

    abstract::Ginkgo biloba L. (known as ginkgo or maidenhair tree) is a phylogenetically isolated, charismatic, gymnosperm tree. Herbal dietary supplements, prepared from G. biloba leaves, are consumed to boost cognitive capacity via improved blood perfusion and mitochondrial function. A novel DNA mini-barcode assay was designed a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2014-0130

    authors: Little DP

    更新日期:2014-09-01 00:00:00

  • The mitochondrial genome of Anopheles quadrimaculatus species A: complete nucleotide sequence and gene organization.

    abstract::The complete sequence (15,455 bp) of the mitochondrial DNA of the mosquito Anopheles quadrimaculatus species A is reported. This genome is compact and very A+T rich (77.4% A+T). It contains genes for 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and 13 subunits of the mitochondrial inner membrane respiratory com...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-141

    authors: Mitchell SE,Cockburn AF,Seawright JA

    更新日期:1993-12-01 00:00:00

  • 5S rDNA and U2 snRNA are linked in the genome of Crassostrea angulata and Crassostrea gigas oysters: does the (CT)n.(GA)n microsatellite stabilize this novel linkage of large tandem arrays?

    abstract::The 5S rRNA genes from 2 species of the Ostreidae family, Crassostrea angulata and Crassostrea gigas, were molecularly characterized. The genes were amplified, cloned, and sequenced. The results revealed a 5S rDNA tandem array with a nucleotide sequence in an inverted position within the nontranscribed spacer region t...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-075

    authors: Cross I,Rebordinos L

    更新日期:2005-12-01 00:00:00

  • Mitochondrial genome analysis of the predatory mite Phytoseiulus persimilis and a revisit of the Metaseiulus occidentalis mitochondrial genome.

    abstract::In this study we sequenced and analysed the complete mitochondrial (mt) genome of the Chilean predatory mite Phytoseiulus persimilis Athias-Henriot (Chelicerata: Acari: Mesostigmata: Phytoseiidae: Amblyseiinae). The 16 199 bp genome (79.8% AT) contains the standard set of 13 protein-coding and 24 RNA genes. Compared w...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g10-004

    authors: Dermauw W,Vanholme B,Tirry L,Van Leeuwen T

    更新日期:2010-04-01 00:00:00

  • Full-length transcriptome analysis and identification of genes involved in asarinin and aristolochic acid biosynthesis in medicinal plant Asarum sieboldii.

    abstract::Asarum sieboldii a well-known traditional Chinese medicinal herb, is used for curing inflammation and ache. It contains both bioactive ingredient asarinin and the toxic compound aristolochic acid. To address further breeding demand, genes involved in the biosynthetic pathways of asarinin and aristolochic acid should b...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0095

    authors: Chen C,Shi X,Zhou T,Li W,Li S,Bai G

    更新日期:2020-12-15 00:00:00

  • Analysis of Brachypodium genomes with genome-wide optical maps.

    abstract::Brachypodium distachyon (n = 5) is a diploid and has been widely used as a genetic model. Brachypodium stacei (n = 10) and B. hybridum (n = 15) are species that are related to B. distachyon, leading to an hypothesis that they are part of a polyploid series based on x = 5. Several lines of evidence suggest that this hy...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0013

    authors: Zhu T,Hu Z,Rodriguez JC,Deal KR,Dvorak J,Vogel JP,Liu Z,Luo MC

    更新日期:2018-08-01 00:00:00

  • The use of RAPD for identifying and classifying Musa germplasm.

    abstract::Using the technique of random amplified polymorphic DNA (RAPD), we have identified 116 amplification products in Musa germplasm using nine primers. This has enabled us to identify RAPD markers that are specific to each of nine genotypes of Musa representing AA, AAA, AAB, ABB, and BB genomes. The pattern of variation o...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-045

    authors: Howell EC,Newbury HJ,Swennen RL,Withers LA,Ford-Lloyd BV

    更新日期:1994-04-01 00:00:00

  • Genome variation in the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti.

    abstract::Differences in genome size and gene content are among the most important signatures of microbial adaptation and genome evolution. Here, we investigated the patterns of genome variation among 10 natural strains of the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti, using pulse field gel electrophoresis (PFG...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g09-060

    authors: Guo H,Sun S,Eardly B,Finan T,Xu J

    更新日期:2009-10-01 00:00:00

  • Molecular cytogenetics identification of a wheat - Leymus mollis double disomic addition line with stripe rust resistance.

    abstract::Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) possesses a number of valuable genes against biotic and abiotic stress, which could be transferred into common wheat background for wheat improvement. In the present study, we determined the karyotypic constitution of a wheat - L. mollis double disomic addition line...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0151

    authors: Zhang A,Li W,Wang C,Yang X,Chen C,Zhu C,Peng N,Tian Z,Wang Y,Zhang H,Liu X,Ji W

    更新日期:2017-05-01 00:00:00

  • Genome evolution of intermediate wheatgrass as revealed by EST-SSR markers developed from its three progenitor diploid species.

    abstract::Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey), a segmental autoallohexaploid (2n = 6x = 42), is not only an important forage crop but also a valuable gene reservoir for wheat (Triticum aestivum L.) improvement. Throughout the scientific literature, there continues to be disagreement as...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2014-0186

    authors: Wang RR,Larson SR,Jensen KB,Bushman BS,DeHaan LR,Wang S,Yan X

    更新日期:2015-02-01 00:00:00

  • Identification and analysis of copine/BONZAI proteins among evolutionarily diverse plant species.

    abstract::Copines are evolutionarily conserved calcium-dependent membrane-binding proteins with potentially critical biological functions. In plants, the function of these proteins has not been analyzed except for in Arabidopsis thaliana where they play critical roles in development and disease resistance. To facilitate functio...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0015

    authors: Zou B,Hong X,Ding Y,Wang X,Liu H,Hua J

    更新日期:2016-08-01 00:00:00

  • Mitochondrial DNA variation within and among populations of the mosquito Aedes albopictus.

    abstract::A survey of restriction fragment polymorphism in mitochondrial DNA (mtDNA) of 17 populations of the mosquito Aedes albopictus was undertaken. The mtDNA size was estimated to be about 17.5 kbp. The level of polymorphism was low, with over 99% of the fragments being shared in common among the 17 populations. Three popul...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g91-046

    authors: Kambhampati S,Rai KS

    更新日期:1991-04-01 00:00:00

  • RAPD markers as predictors of infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance in shrimp (Litopenaeus stylirostris).

    abstract::Random amplified polymorphic DNA (RAPD) fingerprints of two shrimp populations (Litopenaeus stylirostris) were compared to find genetic marker(s) that may be associated with infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance or susceptibility. Of the 100 10-mer random primers and 100 intersimple...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g01-117

    authors: Hizer SE,Dhar AK,Klimpel KR,Garcia DK

    更新日期:2002-02-01 00:00:00

  • Molecular cloning, heterologous expression, and phylogenetic analysis of a novel y-type HMW glutenin subunit gene from the G genome of Triticum timopheevi.

    abstract::A novel y-type high molecular weight (HMW) glutenin subunit gene from the G genome of Triticum timopheevi (2n=4x=28, AAGG) was isolated and characterized. Genomic DNA from accession CWI17006 was amplified and a 2200 bp fragment was obtained. Sequence analysis revealed a complete open reading frame including N- and C-t...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G07-089

    authors: Li X,Zhang Y,Gao L,Wang A,Ji K,He Z,Appels R,Ma W,Yan Y

    更新日期:2007-12-01 00:00:00

  • Segmental chromosomal duplications harbouring group IV CONSTANS-like genes in cereals.

    abstract::Comparative mapping is an important component of map-based cloning in large-genome cereal species. We describe evidence of a segmental chromosomal duplication harbouring CONSTANS-like genes in barley that predates the divergence of the Oryzoideae (rice) and Pooideae (brachypodium, barley, wheat) clades, and discuss th...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g09-101

    authors: Cockram J,Howells RM,O'Sullivan DM

    更新日期:2010-03-01 00:00:00

  • Interactions between the scs and Vi genes in alloplasmic durum wheat.

    abstract::Two nuclear genes, vitality (Vi) on an A- or B-genome chromosome and species cytoplasm specific (scs) on a 1DL telosome from Triticum aestivum L. or a telosome from Aegilops uniaristata Vis. (un telosome), improved compatibility between the nucleus of Triticum turgidum L. var. durum and the cytoplasm of Ae. longissima...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-029

    authors: Maan SS

    更新日期:1994-04-01 00:00:00

  • Computers: the best friends a human genome ever had.

    abstract::Mapping and sequencing the human genome will generate large amounts of data, which must be sorted, analyzed, and stored for rapid retrieval to complete this enormous task. Computers and their software programs provide the most important tool to the molecular biologist today. A discussion of current capabilities and fu...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/g89-177

    authors: Kelly MJ

    更新日期:1989-01-01 00:00:00

  • Genome and transcriptome analysis of the latent pathogen Lasiodiplodia theobromae, an emerging threat to the cacao industry.

    abstract::Lasiodiplodia theobromae (Pat.) Griffon & Maubl., a member of the family Botryosphaeriaceae, is becoming a significant threat to crops and woody plants in many parts of the world, including the major cacao growing areas. While attempting to isolate Ceratobasidium theobromae, a causal agent of vascular streak dieback (...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2019-0112

    authors: Ali SS,Asman A,Shao J,Balidion JF,Strem MD,Puig AS,Meinhardt LW,Bailey BA

    更新日期:2020-01-01 00:00:00

  • Development, characterization, and comparative analysis of polymorphism at common bean SSR loci isolated from genic and genomic sources.

    abstract::Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. In the present study, 3 cDNA libraries generated by the Bean EST project (http://lgm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genom...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g07-007

    authors: Hanai LR,de Campos T,Camargo LE,Benchimol LL,de Souza AP,Melotto M,Carbonell SA,Chioratto AF,Consoli L,Formighieri EF,Siqueira MV,Tsai SM,Vieira ML

    更新日期:2007-03-01 00:00:00

  • Exact word matches in rice pseudomolecules.

    abstract::Using pseudomolecules of assembled genomic sequence, we computed the frequencies of 6 to 24 bp oligonucleotide (oligo) "words" across the genome of rice (Oryza sativa L. subsp. japonica). All oligos of 10 or fewer basepairs were repeated at least 12 times in the genome. The percentage of unique (non-repeated) oligos r...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-055

    authors: Liu S,Tinker NA,Mather DE

    更新日期:2006-08-01 00:00:00

  • A compilation of soybean ESTs: generation and analysis.

    abstract::Whole-genome sequencing is fundamental to understanding the genetic composition of an organism. Given the size and complexity of the soybean genome, an alternative approach is targeted random-gene sequencing, which provides an immediate and productive method of gene discovery. In this study, more than 120000 soybean e...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g01-150

    authors: Shoemaker R,Keim P,Vodkin L,Retzel E,Clifton SW,Waterston R,Smoller D,Coryell V,Khanna A,Erpelding J,Gai X,Brendel V,Raph-Schmidt C,Shoop EG,Vielweber CJ,Schmatz M,Pape D,Bowers Y,Theising B,Martin J,Dante M,Wyl

    更新日期:2002-04-01 00:00:00

  • Visualizing DNA domains and sequences by microscopy: a fifty-year history of molecular cytogenetics.

    abstract::This short review presents a historical perspective of chromosome research during the last 50 years. It shows how molecular knowledge and technology of DNA entered cytogenetics step by step making it now daily practice in almost every modern chromosome lab. A crucial milestone in these decades has been the development...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/g03-107

    authors: de Jong H

    更新日期:2003-12-01 00:00:00

  • A spontaneous segmental deletion from chromosome arm 3DL enhances Fusarium head blight resistance in wheat.

    abstract::Much effort has been directed at identifying sources of resistance to Fusarium head blight (FHB) in wheat. We sought to identify molecular markers for what we hypothesized was a new major FHB resistance locus originating from the wheat cultivar 'Freedom' and introgressed into the susceptible wheat cultivar 'USU-Apogee...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0088

    authors: Garvin DF,Porter H,Blankenheim ZJ,Chao S,Dill-Macky R

    更新日期:2015-11-01 00:00:00

  • Cytological and molecular analysis of centromere misdivision in maize.

    abstract::B chromosome derivatives suffering from breaks within their centromere were examined cytologically and molecularly. We showed by high resolution FISH that misdivision of the centromere of a univalent chromosome can occur during meiosis. The breaks divide the centromere repeat sequence cluster. A telocentric chromosome...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-023

    authors: Kaszás E,Kato A,Birchler JA

    更新日期:2002-08-01 00:00:00

  • A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum x Gossypium barbadense backcross population.

    abstract::An interspecific Gossypium hirsutum x Gossypium barbadense backcross population of 75 BC1 plants was evaluated for 1014 markers. The map consists of 888 loci, including 465 AFLPs, 229 SSRs, 192 RFLPs, and 2 morphological markers, ordered in 37 linkage groups that represent most if not all of the 26 chromosomes, altoge...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-050

    authors: Lacape JM,Nguyen TB,Thibivilliers S,Bojinov B,Courtois B,Cantrell RG,Burr B,Hau B

    更新日期:2003-08-01 00:00:00

  • AFLP targeting of the 1-cM region conferring the vrs1 gene for six-rowed spike in barley, Hordeum vulgare L.

    abstract::Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-073

    authors: He C,Sayed-Tabatabaei BE,Komatsuda T

    更新日期:2004-12-01 00:00:00

  • Sewall Wright and quantitative genetics.

    abstract::Some aspects of Wright's great contribution to quantitative genetics and animal breeding are reviewed in relation to current research and practice. Particular aspects discussed are as follows: the utility of his definition of inbreeding coefficient in terms of the correlation of uniting gametes; the maintenance of gen...

    journal_title:Genome

    pub_type: 传,历史文章,杂志文章,评审

    doi:10.1139/g89-033

    authors: Hill WG

    更新日期:1989-01-01 00:00:00

  • Varying signals of the effects of natural selection during teleost growth hormone gene evolution.

    abstract::The growth hormone (GH) gene of teleost fish exhibits a higher degree of variability compared with other vertebrate groups. However, the different selective constraints at the sequence level are not well understood. In this study, maximum-likelihood (ML) models of codon substitutions were used to investigate Darwinian...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-079

    authors: Ryynänen HJ,Primmer CR

    更新日期:2006-01-01 00:00:00