Abstract:
:Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads of chromosomes of a sorghum plant containing an extra copy of one arm of the sorghum chromosome arbitrarily designated with the letter D. Therefore, the sequence cloned in this BAC must reside in the arm of chromosome D represented by this monotelosome. This demonstrates a novel procedure for physically mapping cloned genes or other single-copy sequences by FISH, sh2 in this case, by using BACs containing their complementary sequences. The results reported herein suggest homology, at least in part, between one arm of chromosome D in sorghum and the long arm of chromosome 3 in maize.
journal_name
Genomejournal_title
Genomeauthors
Gómez MI,Islam-Faridi MN,Woo SS,Czeschin D Jr,Zwick MS,Stelly DM,Price HJ,Schertz KF,Wing RAdoi
10.1139/g97-063subject
Has Abstractpub_date
1997-08-01 00:00:00pages
475-8issue
4eissn
0831-2796issn
1480-3321pii
g97-063journal_volume
40pub_type
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