FISH of a maize sh2-selected sorghum BAC to chromosomes of Sorghum bicolor.

Abstract:

:Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads of chromosomes of a sorghum plant containing an extra copy of one arm of the sorghum chromosome arbitrarily designated with the letter D. Therefore, the sequence cloned in this BAC must reside in the arm of chromosome D represented by this monotelosome. This demonstrates a novel procedure for physically mapping cloned genes or other single-copy sequences by FISH, sh2 in this case, by using BACs containing their complementary sequences. The results reported herein suggest homology, at least in part, between one arm of chromosome D in sorghum and the long arm of chromosome 3 in maize.

journal_name

Genome

journal_title

Genome

authors

Gómez MI,Islam-Faridi MN,Woo SS,Czeschin D Jr,Zwick MS,Stelly DM,Price HJ,Schertz KF,Wing RA

doi

10.1139/g97-063

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

475-8

issue

4

eissn

0831-2796

issn

1480-3321

pii

g97-063

journal_volume

40

pub_type

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