Genetic diversity of Crotalaria germplasm assessed through phylogenetic analysis of EST-SSR markers.

Abstract:

:The genetic diversity of the genus Crotalaria is unknown even though many species in this genus are economically valuable. We report the first study in which polymorphic expressed sequence tag-simple sequence repeat (EST-SSR) markers derived from Medicago and soybean were used to assess the genetic diversity of the Crotalaria germplasm collection. This collection consisted of 26 accessions representing 4 morphologically characterized species. Phylogenetic analysis partitioned accessions into 4 main groups generally along species lines and revealed that 2 accessions were incorrectly identified as Crotalaria juncea and Crotalaria spectabilis instead of Crotalaria retusa. Morphological re-examination confirmed that these 2 accessions were misclassified during curation or conservation and were indeed C. retusa. Some amplicons from Crotalaria were sequenced and their sequences showed a high similarity (89% sequence identity) to Medicago truncatula from which the EST-SSR primers were designed; however, the SSRs were completely deleted in Crotalaria. Highly distinguishing markers or more sequences are required to further classify accessions within C. juncea.

journal_name

Genome

journal_title

Genome

authors

Wang ML,Mosjidis JA,Morris JB,Dean RE,Jenkins TM,Pederson GA

doi

10.1139/g06-027

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

707-15

issue

6

eissn

0831-2796

issn

1480-3321

pii

g06-027

journal_volume

49

pub_type

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