Identification of a new class of recombinant prolamin genes in wheat.

Abstract:

:A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshift mutation was also introduced by the putative recombination event. Hence, the amino acid sequence of the C-terminal region was transformed to a completely new polypeptide. Based on this finding, 7 additional recombinant prolamin genes of similar structure were isolated with specific PCR primers. The 8 chimeric clones seem to be derived from 4 individual gamma-gliadin and 3 LMW-glutenin sequences. These genes show remarkable diversity in size, gliadin:glutenin ratio, frameshift mutations, and sulphur content. The putative functional characteristics of the chimeric polypeptides and problems related to the origin of the encoding genes are discussed.

journal_name

Genome

journal_title

Genome

authors

Nagy IJ,Takács I,Juhász A,Tamás L,Bedo Z

doi

10.1139/g05-042

subject

Has Abstract

pub_date

2005-10-01 00:00:00

pages

840-7

issue

5

eissn

0831-2796

issn

1480-3321

pii

g05-042

journal_volume

48

pub_type

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