Abstract:
:A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshift mutation was also introduced by the putative recombination event. Hence, the amino acid sequence of the C-terminal region was transformed to a completely new polypeptide. Based on this finding, 7 additional recombinant prolamin genes of similar structure were isolated with specific PCR primers. The 8 chimeric clones seem to be derived from 4 individual gamma-gliadin and 3 LMW-glutenin sequences. These genes show remarkable diversity in size, gliadin:glutenin ratio, frameshift mutations, and sulphur content. The putative functional characteristics of the chimeric polypeptides and problems related to the origin of the encoding genes are discussed.
journal_name
Genomejournal_title
Genomeauthors
Nagy IJ,Takács I,Juhász A,Tamás L,Bedo Zdoi
10.1139/g05-042subject
Has Abstractpub_date
2005-10-01 00:00:00pages
840-7issue
5eissn
0831-2796issn
1480-3321pii
g05-042journal_volume
48pub_type
杂志文章相关文献
GENOME文献大全abstract::Gene segregation at six protein loci was analysed in progeny from tetraploid males and females obtained by suppression of first mitosis. The triploid full-sib families from five tetraploid males and the diploid gynogenetic lines from four tetraploid females were examined. The proportions of heterozygous gametes (0.83 ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g88-092
更新日期:1988-08-01 00:00:00
abstract::A genetic linkage map of Brassica rapa L. was constructed using recombinant inbred lines (RILs) derived from a cross between yellow-seeded cultivar Sampad and a yellowish brown seeded inbred line 3-0026.027. The RILs were evaluated for seed color under three conditions: field plot, greenhouse, and controlled growth ch...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-066
更新日期:2012-12-01 00:00:00
abstract::This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-117
更新日期:1996-10-01 00:00:00
abstract::The use of interspecific crosses in breeding is an important strategy in improving the genetic base of the modern cultivated potato, Solanum tuberosum L. Until now, it has normally been interspecific Solanum hybrids that have been morphologically and cytologically characterized. However, little is known about the geno...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-088
更新日期:2006-02-01 00:00:00
abstract::Three long terminal repeat (LTR) sequences of Ty1-copia retrotransposons were identified in cucumber (Cucumis sativus L.) and named Tcs 1, Tcs 2, and Tcs 3. A sequence-specific amplification polymorphism (SSAP) marker system based on these LTR sequences displayed a higher level of polymorphism than AFLPs in cucumber. ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-067
更新日期:2007-09-01 00:00:00
abstract::Environmental DNA (eDNA) is emerging as a potentially powerful tool for inferring species' presence, and hence occupancy, from DNA that is shed into environmental samples such as water. Although eDNA screening has been used to detect DNA from a variety of taxonomic groups, it has not yet been used to identify DNA from...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0196
更新日期:2016-12-01 00:00:00
abstract::The aim of this study was to investigate the differential expression characteristics and the roles of the genome-wide microRNAs (miRNAs) in immunoglobulin A nephropathy (IgAN) kidney tissues. We used Illumina high-throughput sequencing technology to evaluate the miRNAs expression of six biopsy tissues from IgAN and si...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2012-0159
更新日期:2013-03-01 00:00:00
abstract::Using genomic in situ hybridization to chromosomes, we identified introgressed segments in a tetrageneric hybrid of Triticum, Avena, Thinopyrum, and Secale, which conferred high resistance to leaf rust, stem rust, stripe rust, powdery mildew, and root rot to wheat. The disease-resistance traits of the hybrid originate...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-013
更新日期:1997-02-01 00:00:00
abstract::Lobster mushroom is a wild edible mushroom with potential commercial value. It is the product resulting of the infection, most commonly of Russula brevipes, by Hypomyces lactifluorum. This study undertook quantitative polymerase chain reaction analysis of tissues sampled at different infection stages to investigate R....
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0168
更新日期:2018-05-01 00:00:00
abstract::Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify ...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::Insulin-like growth factor 1 (IGF1) regulates growth and metabolic functions in vertebrates. A dinucleotide repeat sequence located at the promoter region of the IGF1 gene has been reported in several vertebrate species and may affect the control of the transcriptional activity of this gene. The genotypes of animals f...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Differences in genome size and gene content are among the most important signatures of microbial adaptation and genome evolution. Here, we investigated the patterns of genome variation among 10 natural strains of the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti, using pulse field gel electrophoresis (PFG...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g09-060
更新日期:2009-10-01 00:00:00
abstract::The NADH-specific and NAD(P)H-bispecific nitrate reductase genes from barley have been cloned and sequenced. To determine if the Nar7 locus encodes the NAD(P)H-bispecific nitrate reductase structural gene, a cross was made between a wild-type cultivar, Morex (Nar7 Nar7), and Az70 (nar7w nar7w), a mutant from the culti...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-093
更新日期:1995-08-01 00:00:00
abstract::The genomic origin of alien chromosomes present in a wheat-Thinopyrum intermedium partial amphiploid TAF46 (2n = 8x = 56) and six derived chromosome addition lines were analyzed by genomic in situ hybridization (GISH) using S genomic DNA from Pseudoroegneria strigosa (2n = 2x = 14, SS) as a probe. The GISH analysis cl...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g99-090
更新日期:1999-12-01 00:00:00
abstract::Ginkgo biloba L. (known as ginkgo or maidenhair tree) is a phylogenetically isolated, charismatic, gymnosperm tree. Herbal dietary supplements, prepared from G. biloba leaves, are consumed to boost cognitive capacity via improved blood perfusion and mitochondrial function. A novel DNA mini-barcode assay was designed a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2014-0130
更新日期:2014-09-01 00:00:00
abstract::Twenty-nine lines with deletions in the Lr19 ('Indis') translocated chromosome segment were used to physically map three Thinopyrum RFLP loci as well as the Sr25 and Sd1 loci. From the data, the relative locations of marker loci on the translocation were determined as: Sd1, Xpsr165, Xpsr105, Xps129, Lr19, Wsp-D1, Sr25...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-126
更新日期:1996-10-01 00:00:00
abstract::The complete sequence (15,455 bp) of the mitochondrial DNA of the mosquito Anopheles quadrimaculatus species A is reported. This genome is compact and very A+T rich (77.4% A+T). It contains genes for 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and 13 subunits of the mitochondrial inner membrane respiratory com...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-141
更新日期:1993-12-01 00:00:00
abstract::Five wheat-barley translocations in a wheat background were characterized through the combination of cytogenetic and molecular genetic approaches. The wheat chromosome segments involved in the translocations were identified using sequential GISH and two-colour FISH with the probes pSc119.2 and pAs1. The barley chromat...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-068
更新日期:2002-12-01 00:00:00
abstract::Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construc...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0153
更新日期:2016-07-01 00:00:00
abstract::Globally, biodiversity is declining as a result of anthropogenic pressures, and this could lead to extinction of some species before they are discovered. The loss of insect taxa is of prime concern, given recent reports of significant declines in the populations of many taxa across the globe. Efforts to document biodi...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2019-0193
更新日期:2020-11-10 00:00:00
abstract::Although little is known about the molecular organization of most genes within heterochromatin, the unusual properties of these chromosomal regions suggest that genes therein may be organized and expressed very differently from those in euchromatin. We report here the cloning, by P transposon tagging, of sequences ass...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g90-062
更新日期:1990-06-01 00:00:00
abstract::Southern hybridization analysis of Indian muntjac genomic DNA with the Eco-Taq bovine genomic protamine probe revealed a simple banding pattern. The pattern of hybridization was identical with that previously observed in the genus Bos. This suggested that the bovine probe specifically hybridized to the Indian muntjac ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g90-068
更新日期:1990-06-01 00:00:00
abstract::We have used nuclear and chloroplast molecular markers to genotype cultivated and wild accessions of Vitis vinifera L. from Tunisia and assess their genetic relationships. Fifty-five distinct genotypes were identified among 80 cultivated accessions, including 18 genotypic groups containing between 2 and 5 accessions p...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-072
更新日期:2004-12-01 00:00:00
abstract::In pseudo-arrhenotokous mites, haploid males develop from fertilized eggs that undergo paternal genome loss (PGL) during early embryogenesis. We present evidence that some of the paternal genome may be retained in males of the predatory mite Typhlodromus pyri Scheuten (Acari: Phytoseiidae). Two reproductively compatib...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-110
更新日期:1995-10-01 00:00:00
abstract::The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reac...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-034
更新日期:1996-04-01 00:00:00
abstract::The aplocheiloid killifish Rivulus marmoratus is the only known self-fertilizing hermaphroditic vertebrate. Most natural populations consist almost entirely of hermaphrodites and comprise arrays of homozygous clones. However, in almost all populations thus far studied, clonal variation, as detected with molecular tech...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-121
更新日期:1997-12-01 00:00:00
abstract::Natural selection and domestication have shaped modern horse populations, resulting in a vast range of phenotypically diverse breeds. Horse breeds are classified into three types (pony, light, and draft) generally based on their body type. Understanding the genetic basis of horse type variation and selective pressures...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2020-0001
更新日期:2020-08-01 00:00:00
abstract::The present work compares the molecular affinities, revealed by GISH, with the analysis of meiotic pairing in intra- and interspecific hybrids between species of Zea obtained in previous works. The joint analysis of these data provided evidence about the evolutionary relationships among the species from the paleopolyp...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0081
更新日期:2015-10-01 00:00:00
abstract::All mRNAs on the yeast mitochondrial genome terminate at a conserved dodecamer sequence 5'-AAUAAUAUUCUU-3'. We have characterized two mutants with altered dodecamers. One contains a deletion of the dodecamer at the end of the var1 gene, and the other contains two adjacent transversions in the dodecamer at the end of t...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g89-134
更新日期:1989-01-01 00:00:00
abstract::Using the technique of random amplified polymorphic DNA (RAPD), we have identified 116 amplification products in Musa germplasm using nine primers. This has enabled us to identify RAPD markers that are specific to each of nine genotypes of Musa representing AA, AAA, AAB, ABB, and BB genomes. The pattern of variation o...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-045
更新日期:1994-04-01 00:00:00