Abstract:
:Southern hybridization analysis of Indian muntjac genomic DNA with the Eco-Taq bovine genomic protamine probe revealed a simple banding pattern. The pattern of hybridization was identical with that previously observed in the genus Bos. This suggested that the bovine probe specifically hybridized to the Indian muntjac protamine gene. The opportunity was thus provided to assign the chromosomal location of the protamine gene in a comparatively simple system. Accordingly, this probe and the corresponding cDNA probe were used for in situ chromosome hybridization and localization. Various parameters affecting specificity and the resolution of hybridization were examined. Subsequent to optimization, the Indian muntjac gene was shown to be autosomal and distally located in the telomeric region of the p arm of chromosome 1.
journal_name
Genomejournal_title
Genomeauthors
Krawetz SA,Herfort MH,Dixon GHdoi
10.1139/g90-068subject
Has Abstractpub_date
1990-06-01 00:00:00pages
459-63issue
3eissn
0831-2796issn
1480-3321journal_volume
33pub_type
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