In situ localization of a mammalian protamine gene: parameters affecting specificity of hybridization.

Abstract:

:Southern hybridization analysis of Indian muntjac genomic DNA with the Eco-Taq bovine genomic protamine probe revealed a simple banding pattern. The pattern of hybridization was identical with that previously observed in the genus Bos. This suggested that the bovine probe specifically hybridized to the Indian muntjac protamine gene. The opportunity was thus provided to assign the chromosomal location of the protamine gene in a comparatively simple system. Accordingly, this probe and the corresponding cDNA probe were used for in situ chromosome hybridization and localization. Various parameters affecting specificity and the resolution of hybridization were examined. Subsequent to optimization, the Indian muntjac gene was shown to be autosomal and distally located in the telomeric region of the p arm of chromosome 1.

journal_name

Genome

journal_title

Genome

authors

Krawetz SA,Herfort MH,Dixon GH

doi

10.1139/g90-068

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

459-63

issue

3

eissn

0831-2796

issn

1480-3321

journal_volume

33

pub_type

杂志文章

相关文献

GENOME文献大全
  • Inheritance and expression patterns of BN28, a low temperature induced gene in Brassica napus, throughout the Brassicaceae.

    abstract::Molecular genetics is becoming an important tool in the breeding and selection of agronomically important traits. BN28 is a low temperature induced gene in Brassicaceae species. PCR and Southern blot analysis indicate that BN28 is polymorphic in the three diploid genomes: Brassica rapa (AA), Brassica nigra (BB), and B...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-089

    authors: Hawkins GP,Nykiforuk CL,Johnson-Flanagan AM,Boothe JG

    更新日期:1996-08-01 00:00:00

  • DNA content of heterochromatin and euchromatin in tomato (Lycopersicon esculentum) pachytene chromosomes.

    abstract::Lycopersicon esculentum (tomato) has a small genome (2C = 1.90 pg of DNA) packaged in 2n = 2x = 24 small acrocentric to metacentric chromosomes. Like the chromosomes of other members of the family Solanaceae, tomato chromosomes have pericentromeric heterochromatin. To determine the fraction of the tomato genome found ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-011

    authors: Peterson DG,Stack SM,Price HJ,Johnston JS

    更新日期:1996-02-01 00:00:00

  • Genetic control and identification of QTLs associated with visual quality traits of field pea (Pisum sativum L.).

    abstract::Visual quality of field pea (Pisum sativum L.) is one of the most important determinants of the market value of the harvested crop. Seed coat color, seed shape, and seed dimpling are the major components of visual seed quality of field pea and are considered as important breeding objectives. The objectives of this res...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g10-117

    authors: Ubayasena L,Bett K,Tar'an B,Warkentin T

    更新日期:2011-04-01 00:00:00

  • Identification of a major locus conferring resistance to powdery mildew (Erysiphe polygoni DC) in mungbean (Vigna radiata L. Wilczek) by QTL analysis.

    abstract::A major locus conferring resistance to the causal organism of powdery mildew, Erysiphe polygoni DC, in mungbean (Vigna radiata L. Wilczek) was identified using QTL analysis with a population of 147 recombinant inbred individuals. The population was derived from a cross between 'Berken', a highly susceptible variety, a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-057

    authors: Humphry ME,Magner T,McIntyre CL,Aitken EA,Liu CJ

    更新日期:2003-10-01 00:00:00

  • Association of morphological and RFLP markers in rice (Oryza sativa L.).

    abstract::Seventeen morphological marker genes were associated with restriction fragment length polymorphism markers in rice by using four F2 populations, each segregating for a few observable traits, and 14 near isogenic lines (NILs), each containing one morphological mutant gene. The location of five genes was confirmed on th...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-073

    authors: Yu ZH,McCouch SR,Tanksley SD,Kinoshita T,Sato S

    更新日期:1995-06-01 00:00:00

  • Genomic diversity and multiple origins of tetraploid (2n = 78, 80) Glycine tomentella.

    abstract::Among 15 wild perennial species of the genus Glycine Willd. subgenus Glycine, G. tomentella is exceptional. It is composed of four cytotypes (2n = 38, 40, 78, 80), is diverse in morphological features, and covers a wide geographical area. The objectives of this study were to uncover the genomic diversity in 78- and 80...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-063

    authors: Kollipara KP,Singh RJ,Hymowitz T

    更新日期:1994-06-01 00:00:00

  • Construction and characterization of two BAC libraries from Brachypodium distachyon, a new model for grass genomics.

    abstract::Brachypodium is well suited as a model system for temperate grasses because of its compact genome and a range of biological features. In an effort to develop resources for genome research in this emerging model species, we constructed 2 bacterial artificial chromosome (BAC) libraries from an inbred diploid Brachypodiu...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-087

    authors: Huo N,Gu YQ,Lazo GR,Vogel JP,Coleman-Derr D,Luo MC,Thilmony R,Garvin DF,Anderson OD

    更新日期:2006-09-01 00:00:00

  • Increased spontaneous DNA damage in Cu/Zn superoxide dismutase (SOD1) deficient Drosophila.

    abstract::The superoxide dismutases (SODs) protect oxygen-using cells against reactive oxygen species, the potentially toxic by-products of respiration, oxidative metabolism, and radiation. We have previously shown that genetic disruption of CuZn SOD (SOD1) in Drosophila imparts a recessive phenotype of reduced lifespan, infert...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-083

    authors: Woodruff RC,Phillips JP,Hilliker AJ

    更新日期:2004-12-01 00:00:00

  • Assignment of a gene that determines erythrocytic guanosine-5'-triphosphate concentration (Gtpc) to mouse chromosome 9.

    abstract::Nine inbred mouse strains surveyed for erythrocytic guanosine-5'-triphosphate (GTP) concentration were found to segregate into two discrete groups. Strains having low GTP levels between 1.4 and 3.4 nmol/10(9) cells were C3H/HeJ, C3H/HeHa, A/J, and WB/ReJ. Strains having high GTP levels between 11.0 and 14.8 nmol/10(9)...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-056

    authors: Jenuth JP,Fung E,Snyder FF

    更新日期:1994-06-01 00:00:00

  • Cytogenetic analysis of a self-fertilizing fish, Rivulus marmoratus: remarkable chromosomal constancy over a vast geographic range.

    abstract::The aplocheiloid killifish Rivulus marmoratus is the only known self-fertilizing hermaphroditic vertebrate. Most natural populations consist almost entirely of hermaphrodites and comprise arrays of homozygous clones. However, in almost all populations thus far studied, clonal variation, as detected with molecular tech...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g97-121

    authors: Sola L,Marzovillo M,Rossi AR,Gornung E,Bressanello S,Turner BJ

    更新日期:1997-12-01 00:00:00

  • Large genomes among caridean shrimp.

    abstract::Recent genome size estimates for Arctic amphipods have revealed the largest genomes known in the Crustacea. Here we provide additional data for 7 species of caridean shrimp collected from the Canadian Arctic and the Gulf of St. Lawrence. Genome sizes were estimated by flow cytometry and haploid C-values ranged from 8....

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g07-108

    authors: Rees DJ,Belzile C,Glémet H,Dufresne F

    更新日期:2008-02-01 00:00:00

  • Mapping of genes expressed in Fusarium graminearum-infected heads of wheat cultivar 'Frontana'.

    abstract::The isolation, physical, and genetic mapping of a group of wheat genes expressed in infected heads of Triticum aestivum 'Frontana' resistant to Fusarium head blight is reported. A cDNA library was built from heads of 'Frontana' through suppressive subtractive hybridization, to enrich for sequences induced by the patho...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-098

    authors: Han FP,Fedak G,Ouellet T,Dan H,Somers DJ

    更新日期:2005-02-01 00:00:00

  • Identification and analysis of copine/BONZAI proteins among evolutionarily diverse plant species.

    abstract::Copines are evolutionarily conserved calcium-dependent membrane-binding proteins with potentially critical biological functions. In plants, the function of these proteins has not been analyzed except for in Arabidopsis thaliana where they play critical roles in development and disease resistance. To facilitate functio...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0015

    authors: Zou B,Hong X,Ding Y,Wang X,Liu H,Hua J

    更新日期:2016-08-01 00:00:00

  • Intraspecific sequence variation in the mitochondrial genome of rainbow trout (Oncorhynchus mykiss).

    abstract::We examined the sequence of a 2214 base pair HindIII fragment from the mitochondrial genome of six rainbow trout. The fragment encodes four proteins and two tRNAs. Sequences for two fish from a single locality were identical. Those from separate localities differed by from 1 to 7 nucleotide substitutions. Of 13 variab...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g90-003

    authors: Beckenbach AT,Thomas WK,Sohrabi H

    更新日期:1990-02-01 00:00:00

  • Genome evolution of intermediate wheatgrass as revealed by EST-SSR markers developed from its three progenitor diploid species.

    abstract::Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey), a segmental autoallohexaploid (2n = 6x = 42), is not only an important forage crop but also a valuable gene reservoir for wheat (Triticum aestivum L.) improvement. Throughout the scientific literature, there continues to be disagreement as...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2014-0186

    authors: Wang RR,Larson SR,Jensen KB,Bushman BS,DeHaan LR,Wang S,Yan X

    更新日期:2015-02-01 00:00:00

  • Primate-specific histone variants.

    abstract::Canonical histones (H2A, H2B, H3, and H4) are present in all eukaryotes where they package genomic DNA and participate in numerous cellular processes, such as transcription regulation and DNA repair. In addition to the canonical histones, there are many histone variants, which have different amino acid sequences, poss...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0094

    authors: Ding D,Nguyen TT,Pang MYH,Ishibashi T

    更新日期:2020-11-27 00:00:00

  • Development of microsatellite markers in Fragaria, their use in genetic diversity analysis, and their potential for genetic linkage mapping.

    abstract::We have developed 21 new microsatellites in the model diploid perennial species Fragaria vesca from an enriched genomic library developed using F. vesca 'Ruegen'. The transferability of the primer pairs to other Fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawber...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-142

    authors: Hadonou AM,Sargent DJ,Wilson F,James CM,Simpson DW

    更新日期:2004-06-01 00:00:00

  • Somatic chimerism, genetic inheritance, and mapping of the fleshless berry (flb) mutation in grapevine (Vitis vinifera L.).

    abstract::The fleshless berry (flb) mutation of grapevine (Vitis vinifera L. 'Ugni Blanc') impairs the differentiation and division of inner mesocarp cells responsible for flesh in grapevine berries. In order to study the inheritance of the mutation and to map the flb locus, 5 segregating populations were created. Progeny plant...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-034

    authors: Fernandez L,Doligez A,Lopez G,Thomas MR,Bouquet A,Torregrosa L

    更新日期:2006-07-01 00:00:00

  • Variations in the heat-induced protein pattern of several Drosophila montium subgroup species (Diptera:Drosophilidae).

    abstract::After temperature elevation, the newly synthesized polypeptides from several Drosophila montium subgroup species, of the melanogaster species group, were analyzed in denaturing acrylamide gels. The pattern obtained is characteristic of the heat shock response already documented for many other Drosophila species, altho...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g97-019

    authors: Konstantopoulou I,Drosopoulou E,Scouras ZG

    更新日期:1997-02-01 00:00:00

  • AFLP targeting of the 1-cM region conferring the vrs1 gene for six-rowed spike in barley, Hordeum vulgare L.

    abstract::Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-073

    authors: He C,Sayed-Tabatabaei BE,Komatsuda T

    更新日期:2004-12-01 00:00:00

  • Chromosomal location of three wheat sequences with homology to pollen allergen encoding, DNA replication regulating, and DNA (cytosine-5)-methyltransferase genes in wheat and rye.

    abstract::Three wheat sequences, shown to be homologous to pollen allergen encoding, DNA replication regulating, and DNA (cytosine-5)-methyltransferase genes were localized on chromosomes using nullisomic-tetrasomic wheat ('Chinese Spring') and wheat-rye ('Chinese Spring'/'Imperial') addition lines. Whereas the loci for the pol...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-154

    authors: Korzun V,Balzer HJ,Balzer A,Bäumlein H,Börner A

    更新日期:1996-12-01 00:00:00

  • DNA barcodes and citizen science provoke a diversity reappraisal for the "ring" butterflies of Peninsular Malaysia (Ypthima: Satyrinae: Nymphalidae: Lepidoptera).

    abstract::The "rings" belonging to the genus Ypthima are amongst the most common butterflies in Peninsular Malaysia. However, the species can be difficult to tell apart, with keys relying on minor and often non-discrete ring characters found on the hindwing. Seven species have been reported from Peninsular Malaysia, but this is...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0156

    authors: Jisming-See SW,Sing KW,Wilson JJ

    更新日期:2016-10-01 00:00:00

  • Genetic diversity of different apricot geographical groups determined by SSR markers.

    abstract::Forty apricot cultivars with different geographic origins belonging to the germplasm collections of St. Istvan University (Budapest, Hungary) and the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were studied by means of SSR markers. The aim of the study was to determine the genetic relatio...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-128

    authors: Romero C,Pedryc A,Muñoz V,Llácer G,Badenes ML

    更新日期:2003-04-01 00:00:00

  • Genetic mapping of agronomic traits in false flax (Camelina sativa subsp. sativa).

    abstract::The crucifer oilseed plant false flax (Camelina sativa subsp. sativa) possesses numerous valuable agronomic attributes that make it attractive as an alternative spring-sown crop for tight crop rotations. The oil of false flax is particularly rich in polyunsaturated C18-fatty acids, making it a valuable renewable feeds...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-117

    authors: Gehringer A,Friedt W,Lühs W,Snowdon RJ

    更新日期:2006-12-01 00:00:00

  • Efficiency of RFLP, RAPD, and AFLP markers for the construction of an intraspecific map of the tomato genome.

    abstract::We have constructed a tomato genetic linkage map based on an intraspecific cross between two inbred lines of Lycopersicon esculentum and L. esculentum var. cerasiforme. The segregating population was composed of 153 recombinant inbred lines. This map is comprised of one morphological, 132 RFLP (restriction fragment le...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Saliba-Colombani V,Causse M,Gervais L,Philouze J

    更新日期:2000-02-01 00:00:00

  • Organization of 5S ribosomal RNA genes in tea (Camellia sinensis).

    abstract::The 5S rRNA genes in the Camellia sinensis (L.) O. Kuntze (tea) genome are arranged as tandem repeat units of 300 and 325 bps. The 2 classes of tandem repeats were discovered by Southern hybridisation of tea genomic DNA with a 5S rRNA gene PCR product. ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-44-1-143

    authors: Singh D,Singh M

    更新日期:2001-02-01 00:00:00

  • The cytogenetics of thelytoky in a predominantly asexual parasitoid wasp with covert sex.

    abstract::Asexual lineages in the parasitoid wasp Lysiphlebus fabarum (Hymenoptera: Braconidae: Aphidiinae) have previously been shown to have occasionally undergone sexual reproduction and recombination with males from related sexual populations. In the present study, the cytogenetic system of asexual females in this species i...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-112

    authors: Belshaw R,Quicke DL

    更新日期:2003-02-01 00:00:00

  • Nematode biodiversity assessments need vouchered databases: A BOLD reference library for plant-parasitic nematodes in the superfamily Criconematoidea.

    abstract::Nematodes are frequently cited as underrepresented in faunistic surveys using DNA barcoding with COI. This underrepresentation is generally attributed to a limited presence of nematodes in DNA databases which, in turn, is often ascribed to structural variability and high evolutionary rates in nematode mitochondrial ge...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2019-0196

    authors: Powers TO,Harris TS,Higgins RS,Mullin PG,Powers KS

    更新日期:2020-06-11 00:00:00

  • Presence of a centromeric filament during meiosis.

    abstract::Spermatocytes at meiotic metaphase I and anaphase I have a characteristic centromeric filament in a variety of vertebrate organisms. This centromeric filament was first demonstrated on mouse spermatocytes and its presence is now extended to spermatocytes from the human, rat, golden hamster, bull, and chicken. The visu...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g91-136

    authors: Solari AJ,Tandler CJ

    更新日期:1991-12-01 00:00:00

  • Genome-wide analysis of the HAK potassium transporter gene family reveals asymmetrical evolution in tobacco (Nicotiana tabacum).

    abstract::Being an essential mineral nutrient, potassium (K+) plays numerous important roles in plant growth and development and determines the yield and quality of crop products. The cellular level of K+ is controlled to a large extent by the K+ transporter, which belongs to the KT/HAK/KUP (HAK) family. However, little is know...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0187

    authors: Song Z,Wu X,Gao Y,Cui X,Jiao F,Chen X,Li Y

    更新日期:2019-04-01 00:00:00