Abstract:
:Globally, biodiversity is declining as a result of anthropogenic pressures, and this could lead to extinction of some species before they are discovered. The loss of insect taxa is of prime concern, given recent reports of significant declines in the populations of many taxa across the globe. Efforts to document biodiversity have met with several challenges, amongst which are the difficulties in using morphological features to discriminate species, especially in insects. DNA barcoding is a rapid and reliable method for species identification and discovery, but choosing appropriate primers to amplify the barcode region without coamplifying contaminants remains a key challenge. We developed and tested a set of primers for PCR amplification of the DNA barcode region of the COI gene in polistine wasps. We tested their efficacy in 36 species of vespid wasps, and the solitary wasp Zethus miniatus Saussure. Samples were obtained from Africa, Americas, Asia and Europe. The polistine-specific primers successfully amplified the barcode region for all polistines tested, without amplifying any Wolbachia present; they also worked with many species from the other Vespidae wasp subfamilies. The new primers are valuable for the discovery and accurate documentation of polistine wasps in the four continents.
journal_name
Genomejournal_title
Genomeauthors
Onah IE,Sumner Sdoi
10.1139/gen-2019-0193subject
Has Abstractpub_date
2020-11-10 00:00:00eissn
0831-2796issn
1480-3321pub_type
杂志文章相关文献
GENOME文献大全abstract::The European cultivated potato, Solanum tuberosum subsp. tuberosum, has 6 related cultivated species and more than 200 wild relatives. In Solanum spp., studies of cytoplasmic organelles have been mainly confined to the plastid DNA composition of cultivated and wild species. In this study, 53 genotypes of 30 potato spe...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-042
更新日期:2007-08-01 00:00:00
abstract::Eucalyptus globulus subsp. globulus Labill. (Tasmanian Blue Gum), native to southeast Australia, is a benchmark species for the pulp and paper industry. We genotyped 397 trees from 16 populations of E. globulus representing the native diversity in Australia using 24 microsatellite loci. Eight genetically distinct grou...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2016-0218
更新日期:2017-09-01 00:00:00
abstract::The results of the development of oil palm (Elaeis guineensis Jacq.) microsatellite markers are given step by step, from the screening of libraries enriched in (GA)n, (GT)n, and (CCG)n simple-sequence repeats (SSRs) to the final characterisation of 21 SSR loci. Also published are primer sequences, estimates of allele ...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::The wheat-alien small segment translocation (SAST) lines carrying the beneficial genes from wild species are useful genetic stocks for wheat improvement. In this study, to introduce the grain hardness-related genes of Haynaldia villosa (L.) Schur. into common wheat (Triticum aesitivum L.), the mature female gametes of...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-051
更新日期:2012-09-01 00:00:00
abstract::Genetic diversity of 33 Elymus caninus accessions was investigated using isozyme, RAPD, and microsatellite markers. The three assays differed in the amount of polymorphism detected. Microsatellites detected the highest polymorphism. Six microsatellite primer pairs generated a total of 74 polymorphic bands (alleles), w...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We hav...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-015
更新日期:2010-05-01 00:00:00
abstract::Wide hybrids have been used in generating genetic maps of many plant species. In this study, genetic and physical mapping was performed on ph1b-induced recombinants of rye chromosome 2R in wheat (Triticum aestivum L.). All recombinants were single breakpoint translocations. Recombination 2RS-2BS was absent from the te...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-089
更新日期:2004-02-01 00:00:00
abstract::We have studied the effect of gamma radiation on recombination frequency for intervals across the cluster of linkage group I in Caenorhabditis elegans. Recombination frequency increased approximately twofold across the dpy-5-unc-13 interval after treatment with 2000 rads (1 rad = 10 mGy) of cobalt 60 gamma radiation. ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g87-079
更新日期:1987-06-01 00:00:00
abstract::Post-glacial origins of woodland caribou (Rangifer tarandus subsp.) on the island of Newfoundland and their relationship to mainland populations have been uncertain. Sequence analysis of 2223 bp of the mitochondrial DNA control region and cytochrome b gene from 233 Newfoundland caribou identified 32 haplotypes in four...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0199
更新日期:2018-08-01 00:00:00
abstract::Some aspects of Wright's great contribution to quantitative genetics and animal breeding are reviewed in relation to current research and practice. Particular aspects discussed are as follows: the utility of his definition of inbreeding coefficient in terms of the correlation of uniting gametes; the maintenance of gen...
journal_title:Genome
pub_type: 传,历史文章,杂志文章,评审
doi:10.1139/g89-033
更新日期:1989-01-01 00:00:00
abstract::We report the characterization of 3 new repetitive sequences from the bivalve mollusc Mytilus galloprovincialis, designated Mg1, Mg2, and Mg3, with monomer lengths of 169, 260, and 70 bp, respectively. The 3 repeats together constitute approximately 7.8% of the M. galloprovincialis genome and were found, together with...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-111
更新日期:2006-11-01 00:00:00
abstract::Primers based on a conserved nucleotide binding site (NBS) found in several cloned plant disease resistance genes were used to amplify DNA fragments from the genome of common bean (Phaseolus vulgaris). Cloning and sequence analysis of these fragments uncovered eight unique classes of disease-resistance related sequenc...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construc...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0153
更新日期:2016-07-01 00:00:00
abstract::We have constructed a tomato genetic linkage map based on an intraspecific cross between two inbred lines of Lycopersicon esculentum and L. esculentum var. cerasiforme. The segregating population was composed of 153 recombinant inbred lines. This map is comprised of one morphological, 132 RFLP (restriction fragment le...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Bruchids (Coleoptera: Bruchidae) can cause serious damage to mungbean and several other leguminous crops and there is a strong association between small seed size and bruchid resistance. In investigating the feasibility of breeding large-seeded cultivars with high levels of bruchid resistance, we studied the relations...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G09-031
更新日期:2009-07-01 00:00:00
abstract::Cytological descriptions are given for two sympatric sibling species, Simulium praelargum IIIL-st and Simulium praelargum IIIL-1.2, from Darjeeling, West Bengal, India. Sibling IIIL-1.2 differs from IIIL-st by a two step fixed included inversion (IIIL-1.2) found on the long arm of chromosome III. Both siblings possess...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2014-0025
更新日期:2014-04-01 00:00:00
abstract::This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-117
更新日期:1996-10-01 00:00:00
abstract::A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshif...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-042
更新日期:2005-10-01 00:00:00
abstract::In multi-cellular organisms, failure to properly regulate cell-cycle progression can result in inappropriate cell death or uncontrolled cell division leading to tumor formation. To guard against such events, conserved regulatory mechanisms called "checkpoints" block progression into mitosis in response to DNA damage a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-051
更新日期:2002-10-01 00:00:00
abstract::Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify ...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::The abundance, genomic organization, species distribution, and structure of 33 distinct families of repetitive DNA in Phytophthora are described. The families were identified by screening a library of Phytophthora infestans DNA for repetitive sequences. These was subsequently characterized within 26 species distribute...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g98-060
更新日期:1998-08-01 00:00:00
abstract::Yams are species of the genus Dioscorea (family Dioscoreaceae), which consists of approximately 630 species. The majority of the world production of yams occurs in Africa with 58.8 million t annually, but they are also produced in the Americas and Asia. The saponins in yams have been reported to possess various proper...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0105
更新日期:2017-03-01 00:00:00
abstract::As part of a project on lentil molecular genetics, the sequence of the 18S-25S ribosomal RNA gene intergenic spacer (IGS) of Lens culinaris Medik. was determined. DNA was cloned after polymerase chain reaction (PCR) amplification. The spacer of 2939 bp was composed of nonrepetitive sequences and four tandem arrays of ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-022
更新日期:2000-08-01 00:00:00
abstract::Wild Thinopyrum grasses are important gene pools for forage and cereal crops. Knowledge of their chromosome organizations is pivotal for efficient utilization of this important gene pool in germplasm enhancement programs. Expressed sequence tags derived simple sequence repeat (EST-SSR) markers for Thinopyrum bessarabi...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G10-088
更新日期:2010-12-01 00:00:00
abstract::The genus Cichorium consists of two widely cultivated species C. intybus (chicory) and C. endivia (endive) and four wild species, C. bottae, C. spinosum, C. calvum, and C. pumilum. A multivariate and an UPGMA (unweighted pair group method average) analysis based on AFLP (amplified fragment length polymorphism) markers...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-024
更新日期:2000-06-01 00:00:00
abstract::The aim of this study was to investigate the differential expression characteristics and the roles of the genome-wide microRNAs (miRNAs) in immunoglobulin A nephropathy (IgAN) kidney tissues. We used Illumina high-throughput sequencing technology to evaluate the miRNAs expression of six biopsy tissues from IgAN and si...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2012-0159
更新日期:2013-03-01 00:00:00
abstract::Red clover is an important forage legume species for temperate regions and very little is known about the genetic organization of its breeding populations. We used random amplified polymorphic DNA (RAPD) genetic markers to address the genetic diversity and the distribution of variation in 20 breeding populations and c...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-030
更新日期:2003-08-01 00:00:00
abstract::The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalents can be identifie...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-018
更新日期:2001-04-01 00:00:00
abstract::Two nuclear genes, vitality (Vi) on an A- or B-genome chromosome and species cytoplasm specific (scs) on a 1DL telosome from Triticum aestivum L. or a telosome from Aegilops uniaristata Vis. (un telosome), improved compatibility between the nucleus of Triticum turgidum L. var. durum and the cytoplasm of Ae. longissima...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-029
更新日期:1994-04-01 00:00:00
abstract::A putative old and ubiquitous interspersed DNA repeat family was identified from TaqI restriction, M13 cloning, and sequencing of the genomic DNA of a Mallard (Anas platyrhynchos), a Muscovy Duck (Cairina moschata), a Toulouse Goose (Anser anser), and a Black Swan (Cygnus atratus). A 425-bp consensus core sequence was...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g91-075
更新日期:1991-06-01 00:00:00