The role of a conserved dodecamer sequence in yeast mitochondrial gene expression.

Abstract:

:All mRNAs on the yeast mitochondrial genome terminate at a conserved dodecamer sequence 5'-AAUAAUAUUCUU-3'. We have characterized two mutants with altered dodecamers. One contains a deletion of the dodecamer at the end of the var1 gene, and the other contains two adjacent transversions in the dodecamer at the end of the reading frame of fit1, a gene within the omega+ allele of the 21S rRNA gene. In each mutant, expression of the respective gene is blocked completely. A dominant nuclear suppressor, SUV3-1, was isolated that suppresses the var1 deletion but is without effect on the fit1 dodecamer mutations. Unexpectedly, however, we found that SUV3-1 blocks expression of the wild-type fit1 allele by blocking processing at its dodecamer. SUV3-1 has pleiotropic effects on mitochondrial gene expression, affecting RNA processing, RNA stability, and translation. Our results suggest that RNA metabolism and translation may be part of a multicomponent complex within mitochondria.

journal_name

Genome

journal_title

Genome

authors

Butow RA,Zhu H,Perlman P,Conrad-Webb H

doi

10.1139/g89-134

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

757-60

issue

2

eissn

0831-2796

issn

1480-3321

journal_volume

31

pub_type

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