Haplotype characterization and markers at the barley Mlo powdery mildew resistance locus as tools for marker-assisted selection.

Abstract:

:Recessive mlo alleles of the barley Mlo gene confer resistance to almost all known isolates of the powdery mildew fungal pathogen targeting barley (Hordeum vulgare). To characterize haplotypes present in the Mlo chromosomal region of cultivated Mlo and mlo barley genotypes, we conducted a polymorphism search in 3 predicted low-copy sequence regions adjacent to the Mlo gene by examining a sample of 4 Mlo and 3 mlo cultivars. Eight single-nucleotide polymorphisms (SNPs) and 1 insertion-deletion (indel) were detected, and easy to use PCR-based markers were developed for typing the SNPs. The PCR markers were used to characterize a collection of 46 Mlo and 25 mlo barley cultivars, identifying 3 distinct mlo-11 haplotypes, 1 mlo-9 haplotype, and 4 Mlo haplotypes. We summarized the haplotype and marker information obtained here and in a previous study to help breeders identify strategies for mlo marker-assisted selection. The ability of the markers to identify mlo-resistant genotypes in segregating populations was demonstrated using 2 resistance-characterized F2 populations derived by 3-way crosses.

journal_name

Genome

journal_title

Genome

authors

Tacconi G,Baldassarre V,Collins NC,Bulgarelli D,Stanca AM,Valè G

doi

10.1139/g06-044

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

864-72

issue

8

eissn

0831-2796

issn

1480-3321

pii

g06-044

journal_volume

49

pub_type

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