Abstract:
:This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.
journal_name
Genomejournal_title
Genomeauthors
Nenno M,Schumann K,Nagl Wdoi
10.1139/g94-144subject
Has Abstractpub_date
1994-12-01 00:00:00pages
1018-21issue
6eissn
0831-2796issn
1480-3321pii
g94-144journal_volume
37pub_type
杂志文章相关文献
GENOME文献大全abstract::A high-resolution G-banding technique was used to identify five metacentrics that characterize Suncus murinus from Sri Lanka. These metacentrics were shown to be the product of Robertsonian fusion of acrocentric chromosomes identical to those in the standard karyotype defined by M.B. Rogatcheva et al. Two of the metac...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-003
更新日期:1997-02-01 00:00:00
abstract::We have used a PCR and cloning strategy to identify Ty1-copia-like retrotransposons in tomato, Lycopersicon esculentum Mill. Using degenerate oligonucleotide primers corresponding to conserved domains of the Ty1-copia retrotransposon reverse transcriptase (RT), fragments of about 260 bp were obtained by PCR amplificat...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-056
更新日期:2000-10-01 00:00:00
abstract::Molecular genetics is becoming an important tool in the breeding and selection of agronomically important traits. BN28 is a low temperature induced gene in Brassicaceae species. PCR and Southern blot analysis indicate that BN28 is polymorphic in the three diploid genomes: Brassica rapa (AA), Brassica nigra (BB), and B...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-089
更新日期:1996-08-01 00:00:00
abstract::Meiosis was studied in two diploid (2n = 2x = 24) siblings of Solanum phureja Juz. et Buk. and in 11 disomic and 2 trisomic descendants. The diploid siblings carry the same heterozygous interchange and either one or two inversions. The frequency of quadrivalents at diakinesis/metaphase I in these clones was 0.56 and 0...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-017
更新日期:1995-02-01 00:00:00
abstract::Polycelis nigra is a free-living simultaneous hermaphroditic flatworm that has amphimictic and pseudogamous parthenogenetic biotypes. Sexual individuals are always diploid (2n = 16) and pseudogamous parthenogens are polyploid (usually triploid). Two types of supernumerary chromosomes are found in parthenogens, those r...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-810
更新日期:1997-12-01 00:00:00
abstract::Species of the Chironomus plumosus group display significant differences in their amount of centromeric heterochromatin. A tandem-repetitive satellite-like DNA has been isolated from C. plumosus. This DNA accounts for a major part of the centromeric heterochromatin. The DNA element has a Sau3AI restriction site ("Sau ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-132
更新日期:1994-12-01 00:00:00
abstract::Natural selection and domestication have shaped modern horse populations, resulting in a vast range of phenotypically diverse breeds. Horse breeds are classified into three types (pony, light, and draft) generally based on their body type. Understanding the genetic basis of horse type variation and selective pressures...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2020-0001
更新日期:2020-08-01 00:00:00
abstract::Multiple reaction monitoring mass spectrometry (MRM-MS) is an emerging technology for blood biomarker verification and validation; however, the results may be influenced by pre-analytical factors. This exploratory study was designed to determine if differences in phlebotomy techniques would significantly affect the ab...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0036
更新日期:2015-12-01 00:00:00
abstract::The wild olive distribution extends from the Mediterranean region to south Asia and Austral Africa. The species is also invasive, particularly in Australia. Here, we investigated the sequence variation at five nuclear single-copy genes in 41 native and invasive accessions of the Mediterranean and African olive subspec...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2014-0001
更新日期:2014-03-01 00:00:00
abstract::Bacteria are essential components of natural environments. They contribute to ecosystem functioning through roles as mutualists and pathogens for larger species, and as key components of food webs and nutrient cycles. Bacterial communities respond to environmental disturbances, and the tracking of these communities ac...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2018-0064
更新日期:2019-03-01 00:00:00
abstract::Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We hav...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-015
更新日期:2010-05-01 00:00:00
abstract::Comparative RFLP linkage maps were constructed using five segregating populations derived from two self-incompatible lines (termed PI 230582 and PI 458314) of diploid tuber-bearing Solanum chacoense Bitt. The analysis was based on 84 RFLP loci identified by 73 different cDNA clones. Distortion of expected Mendelian se...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-078
更新日期:1996-08-01 00:00:00
abstract::Amplified fragment length polymorphism (AFLP) analysis was used in combination with bulked segregant analysis (BSA) to identify molecular markers linked to two cowpea (Vigna unguiculata (L.) Walp.) genes conferring resistance to Striga gesnerioides race 1. After AFLP analysis of an F2 population derived from a cross b...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-043
更新日期:2002-10-01 00:00:00
abstract::In this study we sequenced and analysed the complete mitochondrial (mt) genome of the Chilean predatory mite Phytoseiulus persimilis Athias-Henriot (Chelicerata: Acari: Mesostigmata: Phytoseiidae: Amblyseiinae). The 16 199 bp genome (79.8% AT) contains the standard set of 13 protein-coding and 24 RNA genes. Compared w...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-004
更新日期:2010-04-01 00:00:00
abstract::In combination with oligonucleotides of arbitrary sequence, 5' anchored oligonucleotides based on simple sequence repeats were used in polymerase chain reaction amplifications to produce barley DNA fingerprints. The aim of this work was (i) to develop a simple nonradioactive experimental procedure to reveal polymorphi...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-015
更新日期:1996-02-01 00:00:00
abstract::Disease lesion mimics provide an excellent biological system to study the genetic basis of cell death in plants. Many lesion mimics show variation in phenotype expression in different genetic backgrounds. Our goal was to identify quantitative trait loci (QTL) modifying lesion mimic expression thereby identifying genet...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-046
更新日期:2004-10-01 00:00:00
abstract::Genetic diversity in 20 elite blackgram (Vigna mungo (L.) Hepper) genotypes was studied using microsatellite and AFLP markers. Thirty-six microsatellite markers from azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) were successfully amplified across the 20 blackgram genotypes and 33 microsatellite markers showed po...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g08-107
更新日期:2009-02-01 00:00:00
abstract::Genetic variation in Gir cattle (Bos indicus) has so far not been well characterized. In this study, we used whole genome sequencing of three Gir bulls and a pooled sample from another 11 bulls to identify polymorphisms and loci under selection. A total of 9 990 733 single nucleotide polymorphisms (SNPs) and 604 308 i...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2013-0082
更新日期:2013-10-01 00:00:00
abstract::Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construc...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0153
更新日期:2016-07-01 00:00:00
abstract::A mapping population of Paspalum simplex segregating for apomixis (asexual reproduction through seeds) was screened with AFLPs to find apomixis-linked markers. Four AFLPs linked to apomixis in coupling phase were found. Three of them did not show recombinants among the 87 individuals of the mapping population, whereas...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-014
更新日期:2002-06-01 00:00:00
abstract::The aplocheiloid killifish Rivulus marmoratus is the only known self-fertilizing hermaphroditic vertebrate. Most natural populations consist almost entirely of hermaphrodites and comprise arrays of homozygous clones. However, in almost all populations thus far studied, clonal variation, as detected with molecular tech...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-121
更新日期:1997-12-01 00:00:00
abstract::Expensive and unsustainable fishmeal is increasingly being replaced with cheaper lipids and carbohydrates as sources of energy in aquaculture. Although it is known that the excess of lipids and carbohydrates has negative effects on nutrient utilization, growth, metabolic homeostasis, and health of fish, our current un...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2018-0159
更新日期:2019-02-01 00:00:00
abstract::2B rye plants selected for high (H) or low (L) B transmission rate were studied at pachytene and metaphase I of meiosis to determine the relationship between synapsis, bivalents at metaphase I, and B transmission rate. The results show that the 2 B chromosomes (Bs) form bivalents at pachytene in both the H and L lines...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Southern hybridization analysis of Indian muntjac genomic DNA with the Eco-Taq bovine genomic protamine probe revealed a simple banding pattern. The pattern of hybridization was identical with that previously observed in the genus Bos. This suggested that the bovine probe specifically hybridized to the Indian muntjac ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g90-068
更新日期:1990-06-01 00:00:00
abstract::The chromosome makeup of a 3-year-old crossbred heifer was examined in an attempt to explore the cytogenetic causes of her infertility. The heifer proved to be a mosaic carrying predominantly XY cells and with approximately 30% of cells lacking a normal Y. The minor population included cells carrying a dicentric isoch...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g90-104
更新日期:1990-10-01 00:00:00
abstract::Eighteen accessions of a diploid wild peanut species (Arachis duranensis) were analyzed using morphological, intercrossing, cytological, and RFLP data. Abundant variation was found for morphological characters and for RFLP patterns both between and within accessions, and each accession could be uniquely identified by ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-158
更新日期:1995-12-01 00:00:00
abstract::Doubled haploid (DH) lines together with a cotyledon bioassay were employed for the molecular analysis of resistance to the blackleg fungus Leptosphaeria maculans in the Australian Brassica napus cultivars Shiralee and Maluka. We used bulked segregant analysis to identify 13 RAPD and two RFLP markers linked to the res...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-041
更新日期:1997-06-01 00:00:00
abstract::To investigate the effect of signal molecules and their receptor-associated genes on rat liver regeneration (LR) at the transcriptional level, the associated genes were originally obtained by retrieving the databases and related scientific publications; their expression profiles in rat LR were then checked using the R...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g09-022
更新日期:2009-06-01 00:00:00
abstract::Marsupial mammals show extraordinary karyotype stability, with 2n = 14 considered ancestral. However, macropodid marsupials (kangaroos and wallabies) exhibit a considerable variety of karyotypes, with a hypothesised ancestral karyotype of 2n = 22. Speciation and karyotypic diversity in rock wallabies (Petrogale) is ex...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g98-159
更新日期:1999-06-01 00:00:00
abstract::Hordeum vulgare subsp. spontaneum, the wild progenitor of barley, is a potential source of useful genetic variation for barley breeding programs. The objective of this study was to map quantitative trait loci (QTLs) in an advanced backcross population of barley. A total of 207 BC3 lines were developed using the 2-rowe...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-128
更新日期:2006-05-01 00:00:00