Abstract:
:Comparative RFLP linkage maps were constructed using five segregating populations derived from two self-incompatible lines (termed PI 230582 and PI 458314) of diploid tuber-bearing Solanum chacoense Bitt. The analysis was based on 84 RFLP loci identified by 73 different cDNA clones. Distortion of expected Mendelian segregation ratios was observed; less than 10% of the markers showed a skewed segregation in the gametes forming the F1, hybrid population compared with 30% in the selfed population and 46 and 70% in the two populations produced by anther culture. For the anther culture derived populations, most of the skewed loci were scattered throughout the genome, whereas in the populations derived from selfing, they were found primarily in linkage group 1, around the S locus. In this study, we also found that the rate of meiotic recombination could differ between the male and female gametes produced by our parental lines. Thus, male gametes of line PI 458314 showed significantly less recombination as assessed by the total length of the map (206 cM for male gametes vs. 375 cM for female gametes) and the phenomenon was genome-wide. In contrast, the maps from the gametes of PI 230582 had about the same length, but some linkage groups were longer in the female gametes, while others were longer in the male gametes. Key words : Solanum chacoense, RFLP, anther culture, skewed segregation, self-incompatibility, sex differences in recombination.
journal_name
Genomejournal_title
Genomeauthors
Rivard SR,Cappadocia M,Landry BSdoi
10.1139/g96-078subject
Has Abstractpub_date
1996-08-01 00:00:00pages
611-21issue
4eissn
0831-2796issn
1480-3321pii
g96-078journal_volume
39pub_type
杂志文章相关文献
GENOME文献大全abstract::Genomic in situ hybridization (GISH) is one of the most popular and effective techniques for detecting alien chromatin introgressed into breeding lines; however, GISH analysis alone does not reveal the genetic identity of the alien chromosomes. We previously isolated a set of bacterial artificial chromosomes (BACs) sp...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::In an effort to expand the Gossypium hirsutum L. (cotton) expressed sequence tag (EST) database, ESTs representing a variety of tissues and treatments were sequenced. Assembly of these sequences with ESTs already in the EST database (dbEST, GenBank) identified 9675 cotton sequences not present in GenBank. Statistical ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-115
更新日期:2006-04-01 00:00:00
abstract::Hamster beta-tubulin (detected as a mutant subunit that confers Colcemid resistance) is either not expressed or is underexpressed in Chinese hamster-mouse somatic cell hybrids. This selectivity of tubulin expression suggests that a uniparental mouse spindle might preferentially engage mouse chromosomes and lead to los...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g87-090
更新日期:1987-08-01 00:00:00
abstract::Brucella spp., facultative intracellular pathogens that can persistently colonize animal host cells and cause zoonosis, affect public health and safety. A Brucella strain was isolated from yak in Qinghai Province. To detect whether this isolate could cause an outbreak of brucellosis and to reveal its genetic character...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2019-0101
更新日期:2020-08-01 00:00:00
abstract::The growth hormone (GH) gene of teleost fish exhibits a higher degree of variability compared with other vertebrate groups. However, the different selective constraints at the sequence level are not well understood. In this study, maximum-likelihood (ML) models of codon substitutions were used to investigate Darwinian...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-079
更新日期:2006-01-01 00:00:00
abstract::The isolation, physical, and genetic mapping of a group of wheat genes expressed in infected heads of Triticum aestivum 'Frontana' resistant to Fusarium head blight is reported. A cDNA library was built from heads of 'Frontana' through suppressive subtractive hybridization, to enrich for sequences induced by the patho...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-098
更新日期:2005-02-01 00:00:00
abstract::The timing and rate of genomic variation induced by allopolyploidization in the intergeneric wheat-rye (Triticum spp. - Secale cereale L.) hybrid triticale (x Triticosecale Wittmack) was studied using amplified fragment length polymorphism (AFLP) analyses with 2 sets of primers, EcoRI-MseI (E-M) and PstI-MseI (P-M), w...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-078
更新日期:2006-08-01 00:00:00
abstract::Cla elements are a family of tandem repetitive DNA sequences present in the genome of several Chironomus species. Interspersed clusters of Cla elements are widely distributed all over the chromosomes in C. thummi thummi, while they seem to be limited to the centromeric regions in the closely related subspecies C. t. p...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-077
更新日期:1994-08-01 00:00:00
abstract::Trigeneric hybrids offer opportunities to transfer alien traits into cultivated wheat. In this study, a new trigeneric hybrid involving species of Triticum, Psathyrostachys, and Thinopyrum was synthesized by crossing Triticum aestivum L. (wheat)--Thinopyrum intermedium (Host) Barkworth & D.R. Dewey amphiploid Zhong 3 ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-025
更新日期:2012-05-01 00:00:00
abstract::The ancestor of barley (Hordeum vulgare subsp. spontaneum) may be a source of novel alleles for crop improvement. We developed a set of recombinant chromosome substitution lines (RCSLs) using an accession of H. vulgare subsp. spontaneum (Caesarea 26-24, from Israel) as the donor and Hordeum vulgare subsp. vulgare 'Har...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-080
更新日期:2003-12-01 00:00:00
abstract::Canonical histones (H2A, H2B, H3, and H4) are present in all eukaryotes where they package genomic DNA and participate in numerous cellular processes, such as transcription regulation and DNA repair. In addition to the canonical histones, there are many histone variants, which have different amino acid sequences, poss...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2020-0094
更新日期:2020-11-27 00:00:00
abstract::The complete 16 844 bp mitochondrial genome of Neofelis nebulosa has been sequenced and compared with the complete mitochondrial genomes of Felis catus and the Acinonyx jubatus. The base composition of the mitochondrial genome of N. nebulosa is as follows: A, 5343 bp (31.7%); C, 4441 bp (26.4%); G, 2491 bp (14.8%); T,...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-143
更新日期:2007-02-01 00:00:00
abstract::In order to map the genes conditioning the induction of embryos during our anther culture process, we evaluated F2 plants from three different crosses for their anther culture ability and also performed RFLP analysis on these plants. The results showed that six chromosomal regions appear to be associated with the abil...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-127
更新日期:1995-10-01 00:00:00
abstract::A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshif...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-042
更新日期:2005-10-01 00:00:00
abstract::We sequenced the entire coding region of the mitochondrial genome of Heterodera glycines. The sequence obtained comprised 14.9 kb, with PCR evidence indicating that the entire genome comprised a single, circular molecule of approximately 21-22 kb. The genome is the most T-rich nematode mitochondrial genome reported to...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g11-024
更新日期:2011-07-01 00:00:00
abstract::Forty apricot cultivars with different geographic origins belonging to the germplasm collections of St. Istvan University (Budapest, Hungary) and the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were studied by means of SSR markers. The aim of the study was to determine the genetic relatio...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-128
更新日期:2003-04-01 00:00:00
abstract::We have studied the effect of gamma radiation on recombination frequency for intervals across the cluster of linkage group I in Caenorhabditis elegans. Recombination frequency increased approximately twofold across the dpy-5-unc-13 interval after treatment with 2000 rads (1 rad = 10 mGy) of cobalt 60 gamma radiation. ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g87-079
更新日期:1987-06-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-063
更新日期:1997-08-01 00:00:00
abstract::Genomic DNA from 84 individuals of Excoecaria agallocha from seven mangrove populations were analysed for random amplified polymorphic DNAs (RAPDs) using 16 random 10-mer primers. Polymorphism within populations varied from 20% to 31%. At the interpopulation level, 111/149 (74%) of RAPDs were polymorphic. Restriction ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g99-109
更新日期:2000-02-01 00:00:00
abstract::The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalents can be identifie...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-018
更新日期:2001-04-01 00:00:00
abstract::Somatic karyotypes of the nine annual species of Cicer (2n = 16) were analyzed using C-banding. Highly significant differences in haploid genome length and C-band positive heterochromatin content were observed. The haploid genome length ranged from 20.0 μm in the wild species C. judaicum to 28.7 μm in the cultiv...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-093
更新日期:1994-08-01 00:00:00
abstract::The first SSR-based genetic linkage map of Cucurbita moschata was created by integrating the maps of two F2 populations with one common parent developed from the crosses Waltham Butternut (WB) x Nigerian Local (NL) and ZHOU (a hull-less type) x WB. The integrated C. moschata map comprises 205 SSR markers and two morph...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-072
更新日期:2008-11-01 00:00:00
abstract::The present study constitutes the first attempt to construct a photographic map of the polytene chromosomes of Dacus oleae, a pest of the olive tree that causes serious financial damage in all olive oil producing countries. The map was constructed by using the larval fat body cells, the chromosomes of which are repres...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g92-056
更新日期:1992-06-01 00:00:00
abstract::During 30 years of unprecedented urbanization, plant diversity in Shenzhen, a young megacity in southern China, has increased dramatically. Although strongly associated with plant diversity, butterfly diversity generally declines with urbanization, but this has not been investigated in Shenzhen. Considering the speed ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0192
更新日期:2016-09-01 00:00:00
abstract::Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii, is one of the most important foliar diseases of barley (Hordeum vulgare L.) in North America. The primary problem caused by this disease is substantial yield loss. The objective of this study was to determine the chromosomal location of SSLB resistan...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-032
更新日期:2010-08-01 00:00:00
abstract::The wheat-alien small segment translocation (SAST) lines carrying the beneficial genes from wild species are useful genetic stocks for wheat improvement. In this study, to introduce the grain hardness-related genes of Haynaldia villosa (L.) Schur. into common wheat (Triticum aesitivum L.), the mature female gametes of...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-051
更新日期:2012-09-01 00:00:00
abstract::The aplocheiloid killifish Rivulus marmoratus is the only known self-fertilizing hermaphroditic vertebrate. Most natural populations consist almost entirely of hermaphrodites and comprise arrays of homozygous clones. However, in almost all populations thus far studied, clonal variation, as detected with molecular tech...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-121
更新日期:1997-12-01 00:00:00
abstract::Codons play important roles in regulating gene expression levels and mRNA half-lives. However, codon usage and related studies in multicellular organisms still lag far behind those in unicellular organisms. In this study, we describe for the first time genome-wide patterns of codon bias in Morus notabilis (mulberry tr...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2016-0129
更新日期:2017-06-01 00:00:00
abstract::Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was car...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-023
更新日期:2005-08-01 00:00:00
abstract::2B rye plants selected for high (H) or low (L) B transmission rate were studied at pachytene and metaphase I of meiosis to determine the relationship between synapsis, bivalents at metaphase I, and B transmission rate. The results show that the 2 B chromosomes (Bs) form bivalents at pachytene in both the H and L lines...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00