First isolation and characterization of Brucella suis from yak.

abstract：:The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reac...

journal_title：Genome

authors： El-Kharbotly A,Jacobs JM,Hekkert BT,Stiekema WJ,Pereira A,Jacobsen E,Ramanna MS

更新日期：1996-04-01 00:00:00

abstract：:Chamaecyparis nootkatensis is an ecologically and economically important conifer of the north Pacific coastal forests. To aid in studies of clonal structure and genetic differentiation of this and related species, we isolated and characterized microsatellites from C. nootkatensis. A microsatellite-enriched library yie...

journal_title：Genome

authors： Bérubé Y,Ritland C,Ritland K

更新日期：2003-06-01 00:00:00

abstract：:The octamer-binding transcription factor 4 gene (Oct4) plays a critical role in maintaining pluripotency during early mammalian embryonic development and self-renewal of embryonic stem (ES) cells. In this study, we cloned the Oct4 cDNA and 2.8-kb regulatory region upstream of the start codon in Guanzhong dairy goat ( ...

journal_title：Genome

authors： Cheng X,Meng S,Deng J,Lai W,Wang H

更新日期：2011-10-01 00:00:00

abstract：:In an initial investigation of differential expression of genes caused by cis-acting regulatory elements in rice, the lack of reproducibility led us to question the basic premise of allelic expression imbalance determination: namely that departures of cDNA expression ratios from those observed in genomic DNA provide u...

journal_title：Genome

authors： Akkinepalli H,Ereful N,Liu Y,Malabanan K,Howells R,Stamati K,Powell W,Leung H,Greenland A,Mackay I,Lee D

更新日期：2012-05-01 00:00:00

abstract：:Environmental DNA (eDNA) is emerging as a potentially powerful tool for inferring species' presence, and hence occupancy, from DNA that is shed into environmental samples such as water. Although eDNA screening has been used to detect DNA from a variety of taxonomic groups, it has not yet been used to identify DNA from...

journal_title：Genome

authors： Cho A,Morris T,Wilson C,Freeland J

更新日期：2016-12-01 00:00:00

abstract：:Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as g...

journal_title：Genome

authors： Echt CS,Erdahl LA,McCoy TJ

更新日期：1992-02-01 00:00:00

abstract：:Cashew (Anacardium occidentale L.), introduced into India about 400 years ago, is distributed widely in the coastal regions as spontaneous populations as well as in cultivation. Despite the plant's commercial exploitation, little is known about its actual introduction and diversification. We attempted to construct the...

journal_title：Genome

authors： Archak S,Gaikwad AB,Swamy KR,Karihaloo JL

更新日期：2009-03-01 00:00:00

abstract：:Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands ...

journal_title：Genome

authors： Brooks SA,Huang L,Gill BS,Fellers JP

更新日期：2002-10-01 00:00:00

abstract：:A new method was developed to detect DNA methylation in the Citrus genome using random amplification coupled with restriction enzyme digestion. Genomic DNA from Citrus grandis (L.) Osb., Poncirus trifoliata (L.) Raf., and their F1 hybrid was amplified using 7 individual 10-mer random primers. Prior to amplification th...

journal_title：Genome

authors： Cai Q,Guy CL,Moore GA

更新日期：1996-04-01 00:00:00

abstract：:The wild olive distribution extends from the Mediterranean region to south Asia and Austral Africa. The species is also invasive, particularly in Australia. Here, we investigated the sequence variation at five nuclear single-copy genes in 41 native and invasive accessions of the Mediterranean and African olive subspec...

journal_title：Genome

authors： Besnard G,El Bakkali A

更新日期：2014-03-01 00:00:00

abstract：:Mapping and sequencing the human genome will generate large amounts of data, which must be sorted, analyzed, and stored for rapid retrieval to complete this enormous task. Computers and their software programs provide the most important tool to the molecular biologist today. A discussion of current capabilities and fu...

journal_title：Genome

authors： Kelly MJ

更新日期：1989-01-01 00:00:00

abstract：:B chromosome derivatives suffering from breaks within their centromere were examined cytologically and molecularly. We showed by high resolution FISH that misdivision of the centromere of a univalent chromosome can occur during meiosis. The breaks divide the centromere repeat sequence cluster. A telocentric chromosome...

journal_title：Genome

authors： Kaszás E,Kato A,Birchler JA

更新日期：2002-08-01 00:00:00

abstract：:Restriction endonuclease TaqI has been known as a nonbanding restriction endonuclease when it is used on fixed human chromosomes. However, a specific TaqI digestion can be obtained after varying experimental conditions such as concentration of enzyme, time of incubation, and volume of the final reaction mixture. This ...

journal_title：Genome

authors： Tagarro I,González-Aguilera JJ,Fernández-Peralta AM

更新日期：1991-04-01 00:00:00

abstract：:Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with 4 genotypes of known Vrn genes, using previ...

journal_title：Genome

authors： Iqbal M,Navabi A,Yang RC,Salmon DF,Spaner D

更新日期：2007-05-01 00:00:00

abstract：:Microsatellites have many desirable marker properties and have been increasingly used in crop plants in genetic diversity studies. Here we report on the characterisation of microsatellite markers and on their use for the determination of genetic identities and the assessment of genetic variability among accessions fro...

journal_title：Genome

authors： Jakse J,Kindlhofer K,Javornik B

更新日期：2001-10-01 00:00:00

abstract：:Changes in chromosome number have played an important role in the evolution of the genus Trifolium. Along with a few species of polyploid origin there are several cases of dysploidy as evidenced by the presence of four basic chromosome numbers (x = 8, 7, 6, 5). Trifolium subterraneum and Trifolium israeliticum are rel...

journal_title：Genome

authors： Falistocco E,Marconi G,Falcinelli M

更新日期：2013-06-01 00:00:00

abstract：:Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was car...

journal_title：Genome

authors： Yasukochi Y,Banno Y,Yamamoto K,Goldsmith MR,Fujii H

更新日期：2005-08-01 00:00:00

abstract：:Differences in genome size and gene content are among the most important signatures of microbial adaptation and genome evolution. Here, we investigated the patterns of genome variation among 10 natural strains of the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti, using pulse field gel electrophoresis (PFG...

journal_title：Genome

authors： Guo H,Sun S,Eardly B,Finan T,Xu J

更新日期：2009-10-01 00:00:00

abstract：:The first doubled haploid oat linkage map constructed at MTT Agrifood Research Finland was supplemented with additional microsatellites and Diversity Array Technology (DArT) markers to produce a map containing 1058 DNA markers and 34 linkage groups. The map was used to locate quantitative trait loci (QTLs) for 11 impo...

journal_title：Genome

authors： Tanhuanpää P,Manninen O,Beattie A,Eckstein P,Scoles G,Rossnagel B,Kiviharju E

更新日期：2012-04-01 00:00:00

abstract：:Recessive mlo alleles of the barley Mlo gene confer resistance to almost all known isolates of the powdery mildew fungal pathogen targeting barley (Hordeum vulgare). To characterize haplotypes present in the Mlo chromosomal region of cultivated Mlo and mlo barley genotypes, we conducted a polymorphism search in 3 pred...

journal_title：Genome

authors： Tacconi G,Baldassarre V,Collins NC,Bulgarelli D,Stanca AM,Valè G

更新日期：2006-08-01 00:00:00

abstract：:Comparative RFLP linkage maps were constructed using five segregating populations derived from two self-incompatible lines (termed PI 230582 and PI 458314) of diploid tuber-bearing Solanum chacoense Bitt. The analysis was based on 84 RFLP loci identified by 73 different cDNA clones. Distortion of expected Mendelian se...

journal_title：Genome

authors： Rivard SR,Cappadocia M,Landry BS

更新日期：1996-08-01 00:00:00

abstract：:In the root meristem cells of the rice line AMR, which causes loss of heterozygosity in its hybrids, both normal and assortment mitoses were observed. During normal mitosis, chromosomes did not form homologous pairs at metaphase; all chromosomes lined up at the equatorial plate and 2 chromatids of each chromosome disj...

journal_title：Genome

authors： Wang RR,Li X,Chatterton NJ

更新日期：2006-05-01 00:00:00

abstract：:Yams are species of the genus Dioscorea (family Dioscoreaceae), which consists of approximately 630 species. The majority of the world production of yams occurs in Africa with 58.8 million t annually, but they are also produced in the Americas and Asia. The saponins in yams have been reported to possess various proper...

journal_title：Genome

authors： Techen N,Parveen I,Khan IA

更新日期：2017-03-01 00:00:00

abstract：:Knowledge of the inheritance of C-glycosyl flavone synthesis in maize (Zea mays L.) silk tissues has been acquired through detailed genetic studies involving primarily germplasm from the Corn Belt Dent race. To test the robustness of this genetic knowledge, we examined C-glycosyl flavone synthesis in a genetically dis...

journal_title：Genome

authors： Lee EA,Staebler JM,Grainger C,Snook ME

更新日期：2009-01-01 00:00:00

abstract：:Basic helix-loop-helix (bHLH) proteins are highly conserved DNA-binding transcription factors of a large superfamily. Animal bHLH proteins play important regulatory roles in various developmental processes such as neurogenesis, myogenesis, heart development, and hematopoiesis. The jewel wasp (Nasonia vitripennis) is a...

journal_title：Genome

authors： Liu XT,Wang Y,Wang XH,Tao XF,Yao Q,Chen KP

更新日期：2014-10-01 00:00:00

abstract：:The use of complementary RNA sequences such as antisense RNAs and ribozymes to regulate the expression of specific genes in eukaryotic cells has been well-documented, particularly with their application to both human gene therapy and plant biotechnology. Despite the simplicity of this approach, this technique usually ...

journal_title：Genome

authors： Arndt GM,Rank GH

更新日期：1997-12-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP) analysis was used in combination with bulked segregant analysis (BSA) to identify molecular markers linked to two cowpea (Vigna unguiculata (L.) Walp.) genes conferring resistance to Striga gesnerioides race 1. After AFLP analysis of an F2 population derived from a cross b...

journal_title：Genome

authors： Ouédraogo JT,Tignegre JB,Timko MP,Beizile FJ

更新日期：2002-10-01 00:00:00

abstract：:Pigeon spermatocytes were processed with a drying-down technique and their synaptonemal complex (SC) complements were analyzed by electron microscopy. The synaptonemal complex karyotype of the macrobivalents shows an excellent correspondence with the mitotic karyotype. The number and distribution of recombination nodu...

journal_title：Genome

authors： Pigozzi MI,Solari AJ

更新日期：1999-04-01 00:00:00

abstract：:Resistance to common root rot and black point, caused by Cochliobolus sativus, was evaluated in alien chromosome substitution and addition lines of the cultivars 'Cadet' and 'Rescue'. Substitution of chromosome 5B in 'Rescue' with 5Ag from Agropyron elongatum decreased root rot susceptibility to a level intermediate b...

journal_title：Genome

authors： Conner RL,Whelan ED,Laroche A,Thomas JB

更新日期：1993-02-01 00:00:00

abstract：:Random amplified polymorphic DNA (RAPD) fingerprints of two shrimp populations (Litopenaeus stylirostris) were compared to find genetic marker(s) that may be associated with infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance or susceptibility. Of the 100 10-mer random primers and 100 intersimple...

journal_title：Genome

authors： Hizer SE,Dhar AK,Klimpel KR,Garcia DK

更新日期：2002-02-01 00:00:00