Comparative cytogenetic study on Trifolium subterraneum (2n = 16) and Trifolium israeliticum (2n = 12).


:Changes in chromosome number have played an important role in the evolution of the genus Trifolium. Along with a few species of polyploid origin there are several cases of dysploidy as evidenced by the presence of four basic chromosome numbers (x = 8, 7, 6, 5). Trifolium subterraneum and Trifolium israeliticum are related species with chromosome complements 2n = 16 and 2n = 12, respectively. Although they represent an interesting case of speciation based on chromosome number reduction, no attempts to demonstrate their cytogenetic affinity have been carried out to date. With this study we performed a comparative cytogenetic study with the purpose of clarifying the evolutionary relationship between these species and to verify whether genomic rearrangements, other than modification of the chromosome number, are associated with the speciation process. Although karyomorphological analysis supports the hypothesis that chromosome rearrangements had a role in the reduction of the chromosome number, the physical mapping of the rDNA sequences revealed a significant remodelling of the 45S and 5S rDNA sites that greatly contributed to the differentiation of the 2n = 16 and 2n = 12 karyotypes. The nucleotide analysis of 5S rDNA repeats confirmed that the two species are related but constitute distinct entities. The observed genomic changes lead to the hypothesis that the 2n = 12 species is the result of an evolutionary pathway that passed through intermediate forms. It cannot be excluded that the most direct ancestor of T. israeliticum is a species with 2n = 14.






Falistocco E,Marconi G,Falcinelli M




Has Abstract


2013-06-01 00:00:00














  • Nematode biodiversity assessments need vouchered databases: A BOLD reference library for plant-parasitic nematodes in the superfamily Criconematoidea.

    abstract::Nematodes are frequently cited as underrepresented in faunistic surveys using DNA barcoding with COI. This underrepresentation is generally attributed to a limited presence of nematodes in DNA databases which, in turn, is often ascribed to structural variability and high evolutionary rates in nematode mitochondrial ge...


    pub_type: 杂志文章


    authors: Powers TO,Harris TS,Higgins RS,Mullin PG,Powers KS

    更新日期:2020-06-11 00:00:00

  • Polytene chromosome relationships of five species of the Anopheles dirus complex in Thailand.

    abstract::Photographic maps and rearrangements of each salivary gland polytene chromosome arm of Anopheles nemophilous (species F) and of An. dirus species A, B, C, and D of the Dirus group from natural populations in Thailand are presented. Structural conformation of heterokaryotypes and comparison of chromosome banding sequen...


    pub_type: 杂志文章


    authors: Poopittayasataporn A,Baimai V

    更新日期:1995-06-01 00:00:00

  • Differential susceptibility of a few members of the nasuta-albomicans complex of Drosophila to paraquat-induced lethality and oxidative stress.

    abstract::The evolution of karyotypically stabilized short-lived (SL) and long-lived (LL) cytoraces in the laboratory have been established and validated through our previous lifespan studies. In the present investigation, we examined the possible reason(s) for the differential longevity among selected members of SL and LL cyto...


    pub_type: 杂志文章


    authors: Ranjini MS,Hosamani R,Muralidhara,Ramachandra NB

    更新日期:2011-10-01 00:00:00

  • Isolation of a new species-specific repetitive sequence from Thinopyrum elongatum and its use in the studies of alien translocations.

    abstract::A new repetitive sequence that is extremely abundant and well dispersed in the Thinopyrum elongatum genome but present in low-copy number in wheat (Triticum aestivum L.) has been isolated. This repeat and a Th. elongatum repeat isolated in another laboratory were used to identify cosmid genomic clones containing the r...


    pub_type: 杂志文章


    authors: Bournival B,Obanni M,Abad A,Ohm H,Mackenzie S

    更新日期:1994-02-01 00:00:00

  • BKm minisatellite sequences are not sex associated but reveal DNA fingerprint polymorphisms in rainbow trout.

    abstract::GATA-GACA repetitive sequences first isolated from a female snake (termed BKm sequences) and associated with sex chromosomes in some species were hybridized to DNA from rainbow trout (Salmo gairdneri). Genomic DNA was studied from three groups of rainbow trout: (i) randomly selected males and females from an outbred g...


    pub_type: 杂志文章


    authors: Lloyd MA,Fields MJ,Thorgaard GH

    更新日期:1989-10-01 00:00:00

  • Molecular evolution of ribosomal intergenic spacers in Odontophrynus americanus 2n and 4n (Amphibia: Anura).

    abstract::Ribosomal intergenic spacers (IGSs) of Odontophrynus americanus 2n and 4n were cloned, restriction mapped, and partially sequenced. Three distinct regions, namely alpha, beta, and delta, were identified in the IGSs. The alpha and beta regions flanked the 28S and 18S rRNA genes, respectively, conserving an identical re...


    pub_type: 杂志文章


    authors: Alvares LE,Polanco C,Brison O,Coutinho LL,Ruiz IR

    更新日期:2002-02-01 00:00:00

  • Integration of molecular and classical linkage groups of the silkworm, Bombyx mori (n = 28).

    abstract::Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was car...


    pub_type: 杂志文章


    authors: Yasukochi Y,Banno Y,Yamamoto K,Goldsmith MR,Fujii H

    更新日期:2005-08-01 00:00:00

  • Analysis of 106 kb of contiguous DNA sequence from the D genome of wheat reveals high gene density and a complex arrangement of genes related to disease resistance.

    abstract::Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands ...


    pub_type: 杂志文章


    authors: Brooks SA,Huang L,Gill BS,Fellers JP

    更新日期:2002-10-01 00:00:00

  • Low-copy microsatellite markers for Pinus taeda L.

    abstract::Eighteen low-copy and genomic microsatellite markers were tested for Mendelian inheritance and then assayed in 41 Pinus taeda L. samples drawn from five regions in the southern United States. The PCR products had multiple alleles, high levels of polymorphism, and little non-specific priming. Fifteen of the 18 markers ...


    pub_type: 杂志文章


    authors: Elsik CG,Minihan VT,Hall SE,Scarpa AM,Williams CG

    更新日期:2000-06-01 00:00:00

  • Evolutionary distribution of light-harvesting complex-like proteins in photosynthetic eukaryotes.

    abstract::Light-harvesting-like (LIL) proteins are low-molecular-mass membrane proteins related to the light-harvesting complexes, which form the dominant antenna system in most photosynthetic eukaryotes. To analyze the LIL protein family, we mined a number of publicly available databases to identify members of this family in a...


    pub_type: 杂志文章


    authors: Neilson JA,Durnford DG

    更新日期:2010-01-01 00:00:00

  • Tomato chromosome 6: effect of alien chromosomal segments on recombinant frequencies.

    abstract::Variation in recombinant frequencies at two adjacent intervals on chromosome 6 of tomato (Lycopersicon esculentum Mill.) has been studied in seven lines that differ in the amount and origin of introgressed segments from wild species. These lines were all crossed to a genotype homozygous recessive for the markers tl, y...


    pub_type: 杂志文章


    authors: Liharska T,Wordragen M,Kammen A,Zabel P,Koornneef M

    更新日期:1996-06-01 00:00:00

  • The interspersed DNA repeat conserved in water birds' genomes.

    abstract::A putative old and ubiquitous interspersed DNA repeat family was identified from TaqI restriction, M13 cloning, and sequencing of the genomic DNA of a Mallard (Anas platyrhynchos), a Muscovy Duck (Cairina moschata), a Toulouse Goose (Anser anser), and a Black Swan (Cygnus atratus). A 425-bp consensus core sequence was...


    pub_type: 杂志文章


    authors: Zhu X,Burgoyne LA,Skinner JD

    更新日期:1991-06-01 00:00:00

  • Large-scale development of functional markers in Brassica species.

    abstract::Numerous quantitative trait loci (QTL) have been detected in Brassica species, but fine-mapping of major QTL has advanced slowly. The development of functional markers can overcome this barrier. We used publicly available PlantGDB-assembled unique transcripts (PUTs) from Brassica species to design 7836 functional simp...


    pub_type: 杂志文章


    authors: An Z,Gao C,Li J,Fu D,Tang Z,Ortegón O

    更新日期:2011-09-01 00:00:00

  • The mitochondrial genome structure of the clouded leopard (Neofelis nebulosa).

    abstract::The complete 16 844 bp mitochondrial genome of Neofelis nebulosa has been sequenced and compared with the complete mitochondrial genomes of Felis catus and the Acinonyx jubatus. The base composition of the mitochondrial genome of N. nebulosa is as follows: A, 5343 bp (31.7%); C, 4441 bp (26.4%); G, 2491 bp (14.8%); T,...


    pub_type: 杂志文章


    authors: Wu X,Zheng T,Jiang Z,Wei L

    更新日期:2007-02-01 00:00:00

  • Genomic diversity and multiple origins of tetraploid (2n = 78, 80) Glycine tomentella.

    abstract::Among 15 wild perennial species of the genus Glycine Willd. subgenus Glycine, G. tomentella is exceptional. It is composed of four cytotypes (2n = 38, 40, 78, 80), is diverse in morphological features, and covers a wide geographical area. The objectives of this study were to uncover the genomic diversity in 78- and 80...


    pub_type: 杂志文章


    authors: Kollipara KP,Singh RJ,Hymowitz T

    更新日期:1994-06-01 00:00:00

  • The use of RAPD for identifying and classifying Musa germplasm.

    abstract::Using the technique of random amplified polymorphic DNA (RAPD), we have identified 116 amplification products in Musa germplasm using nine primers. This has enabled us to identify RAPD markers that are specific to each of nine genotypes of Musa representing AA, AAA, AAB, ABB, and BB genomes. The pattern of variation o...


    pub_type: 杂志文章


    authors: Howell EC,Newbury HJ,Swennen RL,Withers LA,Ford-Lloyd BV

    更新日期:1994-04-01 00:00:00

  • Development of soybean aphid genomic SSR markers using next generation sequencing.

    abstract::Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid (Aphis glycines Matsumura) has become the most damaging insect pest of...


    pub_type: 杂志文章


    authors: Jun TH,Michel AP,Mian MA

    更新日期:2011-05-01 00:00:00

  • Phaseolin nucleotide sequence diversity in Phaseolus. I. Intraspecific diversity in Phaseolus vulgaris.

    abstract::Most information about the molecular biology of phaseolin, the major seed storage protein in Phaseolus vulgaris, has been obtained from the T-type phaseolin, which is characteristic of the Andean gene pool of the species. In the work reported here, two cDNA clones for the S-type phaseolin representing the other major,...


    pub_type: 杂志文章


    authors: Kami JA,Gepts P

    更新日期:1994-10-01 00:00:00

  • Development of a selection index to improve market value of cultured Atlantic salmon (Salmo salar).

    abstract::Genetic parameters were estimated on growth and development traits using analyses of variance and covariance of 42 full-sib families from a select line, 39 full-sib families from a control line of the same strain, and 37 full-sib families from another unselected strain. The traits included percent 1+ smolts, percent s...


    pub_type: 杂志文章


    authors: O'Flynn FM,Friars GW,Bailey JK,Terhune JM

    更新日期:1992-04-01 00:00:00

  • Mapping of quantitative trait loci controlling broomrape (Orobanche crenata Forsk.) resistance in faba bean (Vicia faba L.).

    abstract::Orobanche crenata Forsk. is a root parasite that produces devastating effects on many crop legumes and has become a limiting factor for faba bean production in the Mediterranean region. The efficacy of available control methods is minimal and breeding for broomrape resistance remains the most promising method of contr...


    pub_type: 杂志文章


    authors: Román B,Torres AM,Rubiales D,Cubero JI,Satovic Z

    更新日期:2002-12-01 00:00:00

  • A family of centromeric satellite DNAs from the European brown frog Rana graeca italica.

    abstract::Digestion of Rana graeca italica DNA with Asp 718I produces highly repetitive fragments of 281 and 385 bp that were cloned and sequenced. The shorter fragment corresponds to the unit repeat (RgiS1b) of a satellite DNA. The longer fragment was found to be part of a 494-bp repeat of another satellite DNA (RgiS1a) that w...


    pub_type: 杂志文章


    authors: Cardone DE,Feliciello I,Marotta M,Rosati C,Chinali G

    更新日期:1997-10-01 00:00:00

  • Microsatellites in the silkworm, Bombyx mori: abundance, polymorphism, and strain characterization.

    abstract::We have isolated and characterized microsatellites (simple sequence repeat (SSR) loci) from the silkworm genome. The screening of a partial genomic library by the conventional hybridization method led to the isolation of 28 microsatellites harbouring clones. The abundance of (CA)n repeats in the silkworm genome was ak...


    pub_type: 杂志文章


    authors: Reddy KD,Abraham EG,Nagaraju J

    更新日期:1999-12-01 00:00:00

  • Development of species-specific primers with potential for amplifying eDNA from imperilled freshwater unionid mussels.

    abstract::Environmental DNA (eDNA) is emerging as a potentially powerful tool for inferring species' presence, and hence occupancy, from DNA that is shed into environmental samples such as water. Although eDNA screening has been used to detect DNA from a variety of taxonomic groups, it has not yet been used to identify DNA from...


    pub_type: 杂志文章


    authors: Cho A,Morris T,Wilson C,Freeland J

    更新日期:2016-12-01 00:00:00

  • Identification and comparison of individual chromosomes of three accessions of Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH.

    abstract::Karyotypes of three accessions of Hordeum chilense (H1, H16, and H7), Hordeum vulgare, and Triticum aestivum were characterized by physical mapping of several repetitive sequences. A total of 14 repetitive sequences were used as probes for fluorescence in situ hybridization (FISH) with the aim of identifying inter- an...


    pub_type: 杂志文章


    authors: Rey MD,Moore G,Martín AC

    更新日期:2018-06-01 00:00:00

  • Characterisation and physical localisation of Ty1-copia-like retrotransposons in four Alstroemeria species.

    abstract::The genus Alstroemeria contains species with large genomes (2C = 36.5-78.9 pg (17,600-38,000 Mb) in those species with 2n = 2x = 16). We investigated the diversity and genomic and chromosomal organisation of Ty1-copia-like retrotransposons in four Alstroemeria species. Analysis of 33 PCR-amplified sequences correspond...


    pub_type: 杂志文章


    authors: Kuipers AG,Heslop-Harrison JS,Jacobsen E

    更新日期:1998-06-01 00:00:00

  • Authentication of official Da-huang by sequencing and multiplex allele-specific PCR of a short maturase K gene.

    abstract::Rhubarb (official Da-huang) is an important medicinal herb in Asia. Many adulterants of official Da-huang have been discovered in Chinese markets in recent years, which has resulted in adverse effects in medicinal treatment. Here, novel molecular markers based on a short maturase K (matK) gene were developed for authe...


    pub_type: 杂志文章


    authors: Xu G,Wang X,Liu C,Li W,Wei S,Liu Y,Cheng X,Liu J

    更新日期:2013-02-01 00:00:00

  • Primate-specific histone variants.

    abstract::Canonical histones (H2A, H2B, H3, and H4) are present in all eukaryotes where they package genomic DNA and participate in numerous cellular processes, such as transcription regulation and DNA repair. In addition to the canonical histones, there are many histone variants, which have different amino acid sequences, poss...


    pub_type: 杂志文章


    authors: Ding D,Nguyen TT,Pang MYH,Ishibashi T

    更新日期:2020-11-27 00:00:00

  • Ty1-copia retrotransposon-based SSAP marker development and its potential in the genetic study of cucurbits.

    abstract::Three long terminal repeat (LTR) sequences of Ty1-copia retrotransposons were identified in cucumber (Cucumis sativus L.) and named Tcs 1, Tcs 2, and Tcs 3. A sequence-specific amplification polymorphism (SSAP) marker system based on these LTR sequences displayed a higher level of polymorphism than AFLPs in cucumber. ...


    pub_type: 杂志文章


    authors: Lou Q,Chen J

    更新日期:2007-09-01 00:00:00

  • An updated doubled haploid oat linkage map and QTL mapping of agronomic and grain quality traits from Canadian field trials.

    abstract::The first doubled haploid oat linkage map constructed at MTT Agrifood Research Finland was supplemented with additional microsatellites and Diversity Array Technology (DArT) markers to produce a map containing 1058 DNA markers and 34 linkage groups. The map was used to locate quantitative trait loci (QTLs) for 11 impo...


    pub_type: 杂志文章


    authors: Tanhuanpää P,Manninen O,Beattie A,Eckstein P,Scoles G,Rossnagel B,Kiviharju E

    更新日期:2012-04-01 00:00:00

  • Genetic and cytological mapping of a "Y-2" translocation in the Mediterranean fruit fly Ceratitis capitata.

    abstract::In this paper we analyze genetically and cytologically a Y-chromosome 2 translocation with several markers, some of which are potentially useful for large scale sex separation. The breakpoint of this Y-2 translocation is located at region 6B on the trichogene polytene chromosome map. It is found that, in strains carry...


    pub_type: 杂志文章


    authors: Cladera JL,Delprat MA

    更新日期:1995-12-01 00:00:00