Analysis of 106 kb of contiguous DNA sequence from the D genome of wheat reveals high gene density and a complex arrangement of genes related to disease resistance.

Abstract:

:Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands rather than distributed at random in the genome. Analysis of gene islands within highly repetitive genomes of plants like wheat remains largely unstudied. The objective of our work was to sequence and characterize a contiguous DNA sequence from chromosome IDS of Aegilops tauschii. An RFLP probe that maps to the Lr21 region of IDS was used to isolate a single BAC. The BAC was sequenced and is 106 kb in length. The contiguous DNA sequence contains a 46-kb retroelement-free gene island containing seven coding sequences. Within the gene island is a complex arrangement of resistance and defense response genes. Overall gene density in this BAC is 1 gene per 8.9 kb. This report demonstrates that wheat and its relatives do contain regions with gene densities similar to that of Arabidopsis.

journal_name

Genome

journal_title

Genome

authors

Brooks SA,Huang L,Gill BS,Fellers JP

doi

10.1139/g02-049

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

963-72

issue

5

eissn

0831-2796

issn

1480-3321

journal_volume

45

pub_type

杂志文章

相关文献

GENOME文献大全
  • Alterations in subtelomeric tandem repeats during early stages of allopolyploidy in wheat.

    abstract::The genomic content of the subtelomeric repeated sequences Spelt1 and Spelt52 was studied by dot, Southern, and in situ hybridization in 11 newly synthesized amphiploids of Aegilops and Triticum, and data were compared with the parental plants. Spelt1 had reduced copy numbers in the first generation of three synthetic...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-044

    authors: Salina EA,Numerova OM,Ozkan H,Feldman M

    更新日期:2004-10-01 00:00:00

  • Full-length transcriptome analysis and identification of genes involved in asarinin and aristolochic acid biosynthesis in medicinal plant Asarum sieboldii.

    abstract::Asarum sieboldii a well-known traditional Chinese medicinal herb, is used for curing inflammation and ache. It contains both bioactive ingredient asarinin and the toxic compound aristolochic acid. To address further breeding demand, genes involved in the biosynthetic pathways of asarinin and aristolochic acid should b...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0095

    authors: Chen C,Shi X,Zhou T,Li W,Li S,Bai G

    更新日期:2020-12-15 00:00:00

  • Centromeric heterochromatin and satellite DNA in the Chironomus plumosus species group.

    abstract::Species of the Chironomus plumosus group display significant differences in their amount of centromeric heterochromatin. A tandem-repetitive satellite-like DNA has been isolated from C. plumosus. This DNA accounts for a major part of the centromeric heterochromatin. The DNA element has a Sau3AI restriction site ("Sau ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-132

    authors: Hankeln T,Fillippova MA,Kiknadze II,Aimanova KG,Schmidt ER

    更新日期:1994-12-01 00:00:00

  • Analysis of codon usage patterns in Morus notabilis based on genome and transcriptome data.

    abstract::Codons play important roles in regulating gene expression levels and mRNA half-lives. However, codon usage and related studies in multicellular organisms still lag far behind those in unicellular organisms. In this study, we describe for the first time genome-wide patterns of codon bias in Morus notabilis (mulberry tr...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0129

    authors: Wen Y,Zou Z,Li H,Xiang Z,He N

    更新日期:2017-06-01 00:00:00

  • Identification of wheat-barley translocations by sequential GISH and two-colour FISH in combination with the use of genetically mapped barley SSR markers.

    abstract::Five wheat-barley translocations in a wheat background were characterized through the combination of cytogenetic and molecular genetic approaches. The wheat chromosome segments involved in the translocations were identified using sequential GISH and two-colour FISH with the probes pSc119.2 and pAs1. The barley chromat...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-068

    authors: Nagy ED,Molnár-Láng M,Linc G,Láng L

    更新日期:2002-12-01 00:00:00

  • Moth sex chromatin probed by comparative genomic hybridization (CGH).

    abstract::Abstract: Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males highlighted the W chromosomes in mitotic plates and the W chromatin in polyploid interphase nuclei of the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-003

    authors: Sahara K,Marec F,Eickhoff U,Traut W

    更新日期:2003-04-01 00:00:00

  • Colocalization of antisense RNAs and ribozymes with their target mRNAs.

    abstract::The use of complementary RNA sequences such as antisense RNAs and ribozymes to regulate the expression of specific genes in eukaryotic cells has been well-documented, particularly with their application to both human gene therapy and plant biotechnology. Despite the simplicity of this approach, this technique usually ...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/g97-802

    authors: Arndt GM,Rank GH

    更新日期:1997-12-01 00:00:00

  • Likelihood estimation of quantitative genetic parameters when selection occurs: models and problems.

    abstract::Conceptual aspects of estimation of genetic components of variance and covariance under selection are discussed, with special attention to likelihood methods. Certain selection processes are described and alternative likelihoods that can be used for analysis are specified. There is a mathematical relationship between ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g89-136

    authors: Gianola D,Fernando RL,Im S,Foulley JL

    更新日期:1989-01-01 00:00:00

  • Primate-specific histone variants.

    abstract::Canonical histones (H2A, H2B, H3, and H4) are present in all eukaryotes where they package genomic DNA and participate in numerous cellular processes, such as transcription regulation and DNA repair. In addition to the canonical histones, there are many histone variants, which have different amino acid sequences, poss...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0094

    authors: Ding D,Nguyen TT,Pang MYH,Ishibashi T

    更新日期:2020-11-27 00:00:00

  • AFLP targeting of the 1-cM region conferring the vrs1 gene for six-rowed spike in barley, Hordeum vulgare L.

    abstract::Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-073

    authors: He C,Sayed-Tabatabaei BE,Komatsuda T

    更新日期:2004-12-01 00:00:00

  • Analysis of genetic diversity in red clover (Trifolium pratense L.) breeding populations as revealed by RAPD genetic markers.

    abstract::Red clover is an important forage legume species for temperate regions and very little is known about the genetic organization of its breeding populations. We used random amplified polymorphic DNA (RAPD) genetic markers to address the genetic diversity and the distribution of variation in 20 breeding populations and c...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-030

    authors: Ulloa O,Ortega F,Campos H

    更新日期:2003-08-01 00:00:00

  • Genetic and molecular features for hepadnavirus and plague infections in the Himalayan marmot.

    abstract::The Himalayan marmot (Marmota himalayana), a natural host and transmitter of plague, is also susceptible to the hepadnavirus infection. To reveal the genetic basis of the hepadnavirus susceptibility and the immune response to plague, we systematically characterized the features of immune genes in Himalayan marmot with...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2019-0161

    authors: Liu B,Bai L,Fu Y,Zhao S,Liu H,Wang R,Wang W,Li Y,Tao Y,Wang Z,Fan J,Liu E

    更新日期:2020-06-01 00:00:00

  • Physical mapping of 5S rDNA loci by direct-cloned biotinylated probes in barley chromosomes.

    abstract::5S rDNA loci have been mapped on barley chromosomes by in situ hybridization using five reciprocal translocation lines. Two kinds of DNA probes covering either the 5S rDNA coding region or the 5S rDNA coding and flanking noncoding regions were used. They were prepared by direct cloning from interphase nuclei and simul...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-013

    authors: Fukui K,Kamisugi Y,Sakai F

    更新日期:1994-02-01 00:00:00

  • The use of RAPD for identifying and classifying Musa germplasm.

    abstract::Using the technique of random amplified polymorphic DNA (RAPD), we have identified 116 amplification products in Musa germplasm using nine primers. This has enabled us to identify RAPD markers that are specific to each of nine genotypes of Musa representing AA, AAA, AAB, ABB, and BB genomes. The pattern of variation o...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-045

    authors: Howell EC,Newbury HJ,Swennen RL,Withers LA,Ford-Lloyd BV

    更新日期:1994-04-01 00:00:00

  • Linkage and mapping analyses of the densonucleosis non-susceptible gene nsd-Z in the silkworm Bombyx mori using SSR markers.

    abstract::In the silkworm Bombyx mori, non-susceptibility to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is located on chromosome 15. Owing to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progeny were used for...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-125

    authors: Li M,Guo Q,Hou C,Miao X,Xu A,Guo X,Huang Y

    更新日期:2006-04-01 00:00:00

  • Genetic and physical mapping of homoeologous recombination points involving wheat chromosome 2B and rye chromosome 2R.

    abstract::Wide hybrids have been used in generating genetic maps of many plant species. In this study, genetic and physical mapping was performed on ph1b-induced recombinants of rye chromosome 2R in wheat (Triticum aestivum L.). All recombinants were single breakpoint translocations. Recombination 2RS-2BS was absent from the te...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-089

    authors: Lukaszewski AJ,Rybka K,Korzun V,Malyshev SV,Lapinski B,Whitkus R

    更新日期:2004-02-01 00:00:00

  • AFLP markers linked to resistance against Striga gesnerioides race 1 in cowpea (Vigna unguiculata).

    abstract::Amplified fragment length polymorphism (AFLP) analysis was used in combination with bulked segregant analysis (BSA) to identify molecular markers linked to two cowpea (Vigna unguiculata (L.) Walp.) genes conferring resistance to Striga gesnerioides race 1. After AFLP analysis of an F2 population derived from a cross b...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-043

    authors: Ouédraogo JT,Tignegre JB,Timko MP,Beizile FJ

    更新日期:2002-10-01 00:00:00

  • Genetic relationship among cultivated and wild grapevine accessions from Tunisia.

    abstract::We have used nuclear and chloroplast molecular markers to genotype cultivated and wild accessions of Vitis vinifera L. from Tunisia and assess their genetic relationships. Fifty-five distinct genotypes were identified among 80 cultivated accessions, including 18 genotypic groups containing between 2 and 5 accessions p...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-072

    authors: Snoussi H,Slimane MH,Ruiz-García L,Martínez-Zapater JM,Arroyo-García R

    更新日期:2004-12-01 00:00:00

  • Quantitative trait loci on barley (Hordeum vulgare L.) chromosome 7 associated with components of winterhardiness.

    abstract::Quantitative trait loci (QTL) controlling traits associated with winterhardiness in barley (field survival, LT50, growth habit, and crown fructan content) were mapped to chromosome 7 in a population of 100 F1-derived doubled haploid lines. The largest QTL effects for all traits were detected in a 21% recombination int...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-009

    authors: Hayes PM,Blake T,Chen TH,Tragoonrung S,Chen F,Pan A,Liu B

    更新日期:1993-02-01 00:00:00

  • Isolation and characterization of novel Ty1-copia-like retrotransposons from lily.

    abstract::Species of the genus Lilium are well known for their large genomes. Although expansion of noncoding repeated DNA is believed to account for this genome size, retroelement del Ty3-gypsy is the only one described so far in the genus Lilium. We isolated Ty1-copia elements from Lilium longiflorum and named them LIREs (lil...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2013-0088

    authors: Lee SI,Park KC,Son JH,Hwang YJ,Lim KB,Song YS,Kim JH,Kim NS

    更新日期:2013-09-01 00:00:00

  • Potential of trispecies bridge crosses and random amplified polymorphic DNA markers for introgression of Medicago daghestanica and M. pironae germplasm into alfalfa (M. sativa).

    abstract::This report describes the production and cytology of the first interspecific hybrids between cultivated alfalfa (Medicago sativa L.) at the diploid level (2n = x = 16) and the diploid (2n = 2x = 16) perennial species M. daghestanica and M. pironae. An ovule-embryo culture technique was required to rescue hybrid embryo...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-080

    authors: McCoy TJ,Echt CS

    更新日期:1993-06-01 00:00:00

  • Development of RNA-seq-based molecular markers for characterizing Thinopyrum bessarabicum and Secale introgressions in wheat.

    abstract::Sequence-based markers have added a new dimension in the efficiency of identifying alien introgressions in wheat. Expressed sequence tag-sequence tagged sites (EST-STS) markers have proved useful in tracing alien chromatin. In this study, we report the development of Thinopyrum bessarabicum- and Secale anatolicum-spec...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0057

    authors: Singh AK,Lo K,Dong C,Zhang P,Trethowan RM,Sharp PJ

    更新日期:2020-11-01 00:00:00

  • Intra-species DNA polymorphism in the tobacco cyst-nematode complex (Globodera tabacum) using AFLP.

    abstract::Amplified fragment length polymorphism (AFLP) was used to obtain information on the within-species genetic variability of the tobacco cyst-nematode (TCN) complex. AFLP was found to be well suited to this type of study. The current classification of TCN was confirmed. Results indicate that the Globodera tabacum solanac...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g01-091

    authors: Marché L,Valette S,Grenier E,Mugniéry D

    更新日期:2001-12-01 00:00:00

  • Genome-wide analysis of microRNAs expression profiling in patients with primary IgA nephropathy.

    abstract::The aim of this study was to investigate the differential expression characteristics and the roles of the genome-wide microRNAs (miRNAs) in immunoglobulin A nephropathy (IgAN) kidney tissues. We used Illumina high-throughput sequencing technology to evaluate the miRNAs expression of six biopsy tissues from IgAN and si...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2012-0159

    authors: Tan K,Chen J,Li W,Chen Y,Sui W,Zhang Y,Dai Y

    更新日期:2013-03-01 00:00:00

  • Polymorphism of PCR-based markers targeting exons, introns, promoter regions, and SSRs in maize and introns and repeat sequences in oat.

    abstract::Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Holland JB,Helland SJ,Sharopova N,Rhyne DC

    更新日期:2001-12-01 00:00:00

  • Genome flux in tomato cell clones cultured in vitro in different physiological equilibria. II. A RAPD analysis of variability.

    abstract::An analysis of the effect of changing physiological conditions on genome evolution in tomato cell populations has been carried out on long-term in vitro cultured clones grown on different auxin-cytokinin equilibria or selected for low-high competence for active defense against Fusarium oxysporum f.sp. lycopersici. RAP...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-107

    authors: Bogani P,Simoni A,Lio' P,Scialpi A,Buiatti M

    更新日期:1996-10-01 00:00:00

  • Identification of a new class of recombinant prolamin genes in wheat.

    abstract::A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshif...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-042

    authors: Nagy IJ,Takács I,Juhász A,Tamás L,Bedo Z

    更新日期:2005-10-01 00:00:00

  • Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa.

    abstract::Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as g...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g92-014

    authors: Echt CS,Erdahl LA,McCoy TJ

    更新日期:1992-02-01 00:00:00

  • An updated doubled haploid oat linkage map and QTL mapping of agronomic and grain quality traits from Canadian field trials.

    abstract::The first doubled haploid oat linkage map constructed at MTT Agrifood Research Finland was supplemented with additional microsatellites and Diversity Array Technology (DArT) markers to produce a map containing 1058 DNA markers and 34 linkage groups. The map was used to locate quantitative trait loci (QTLs) for 11 impo...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g2012-017

    authors: Tanhuanpää P,Manninen O,Beattie A,Eckstein P,Scoles G,Rossnagel B,Kiviharju E

    更新日期:2012-04-01 00:00:00

  • Identification of candidate genes for the seed coat colour change in a Brachypodium distachyon mutant induced by gamma radiation using whole-genome re-sequencing.

    abstract::Brachypodium distachyon has been proposed as a model plant for agriculturally important cereal crops such as wheat and barley. Seed coat colour change from brown-red to yellow was observed in a mutant line (142-3) of B. distachyon, which was induced by chronic gamma radiation. In addition, dwarf phenotypes were observ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0145

    authors: Lee MB,Kim DY,Seo YW

    更新日期:2017-07-01 00:00:00