Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa.

Abstract:

:Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.

journal_name

Genome

journal_title

Genome

authors

Echt CS,Erdahl LA,McCoy TJ

doi

10.1139/g92-014

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

84-7

issue

1

eissn

0831-2796

issn

1480-3321

journal_volume

35

pub_type

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