Abstract:
:Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.
journal_name
Genomejournal_title
Genomeauthors
Echt CS,Erdahl LA,McCoy TJdoi
10.1139/g92-014subject
Has Abstractpub_date
1992-02-01 00:00:00pages
84-7issue
1eissn
0831-2796issn
1480-3321journal_volume
35pub_type
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