An AFLP marker tightly linked to apomixis reveals hemizygosity in a portion of the apomixis-controlling locus in Paspalum simplex.

Abstract:

:A mapping population of Paspalum simplex segregating for apomixis (asexual reproduction through seeds) was screened with AFLPs to find apomixis-linked markers. Four AFLPs linked to apomixis in coupling phase were found. Three of them did not show recombinants among the 87 individuals of the mapping population, whereas the other was more loosely linked. Integrating the AFLP data with those obtained previously with rice RFLP anchor markers, a map was drawn for the chromosome region of P. simplex encompassing apomixis. We cloned the three AFLPs tightly linked with apomixis into plasmid vectors and used them as probes to hybridize the restriction digested DNA of the mapping population. Two of them revealed RFLP bands linked to apomixis together with other alleles, whereas one was proven to belong to a hemizygous portion of the apomixis locus. The total picture resulting from AFLP and RFLP analyses was that a cluster of markers tightly linked with apomixis was detected in P simplex together with two other markers that were more loosely linked. These two markers enclosed a relatively large chromosome segment characterized by strong repression of recombination. The block of recombination may have caused sequence divergence and, therefore, hemizygosity of some regions belonging to the apomixis-controlling chromosome segment of P. simplex. The potential of developing an apomixis-specific sequence for screening large-fragment libraries for the physical isolation of the locus encompassing apomixis is discussed.

journal_name

Genome

journal_title

Genome

authors

Labombarda P,Busti A,Caceres ME,Pupilli F,Arcioni S

doi

10.1139/g02-014

subject

Has Abstract

pub_date

2002-06-01 00:00:00

pages

513-9

issue

3

eissn

0831-2796

issn

1480-3321

journal_volume

45

pub_type

杂志文章

相关文献

GENOME文献大全
  • Development of soybean aphid genomic SSR markers using next generation sequencing.

    abstract::Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid (Aphis glycines Matsumura) has become the most damaging insect pest of...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g11-002

    authors: Jun TH,Michel AP,Mian MA

    更新日期:2011-05-01 00:00:00

  • Phylogenetic analysis of Carthamus species based on the nucleotide sequence of the nuclear SACPD gene and chloroplast trnL-trnF IGS region.

    abstract::To clarify the phylogenetic relationships of Carthamus species, we performed sequence analysis of the nuclear stearoyl acyl carrier protein desaturase (SACPD) gene and the chloroplast intergenic spacer region between leucine and phenylalanine tRNA genes (trnL-trnF IGS) in 13 taxa of Carthamus. The previous division of...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G08-059

    authors: Sasanuma T,Sehgal D,Sasakuma T,Raina SN

    更新日期:2008-09-01 00:00:00

  • Evolutionary distribution of light-harvesting complex-like proteins in photosynthetic eukaryotes.

    abstract::Light-harvesting-like (LIL) proteins are low-molecular-mass membrane proteins related to the light-harvesting complexes, which form the dominant antenna system in most photosynthetic eukaryotes. To analyze the LIL protein family, we mined a number of publicly available databases to identify members of this family in a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g09-081

    authors: Neilson JA,Durnford DG

    更新日期:2010-01-01 00:00:00

  • DNA barcodes and citizen science provoke a diversity reappraisal for the "ring" butterflies of Peninsular Malaysia (Ypthima: Satyrinae: Nymphalidae: Lepidoptera).

    abstract::The "rings" belonging to the genus Ypthima are amongst the most common butterflies in Peninsular Malaysia. However, the species can be difficult to tell apart, with keys relying on minor and often non-discrete ring characters found on the hindwing. Seven species have been reported from Peninsular Malaysia, but this is...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0156

    authors: Jisming-See SW,Sing KW,Wilson JJ

    更新日期:2016-10-01 00:00:00

  • QTL mapping of seed-quality traits in sunflower recombinant inbred lines under different water regimes.

    abstract::The objectives of the present research were to determine the effects of water stress on seed-quality traits and to map QTLs controlling the studied traits under two different water treatments in a population of sunflower recombinant inbred lines (RILs). Two experiments were conducted in greenhouse and field conditions...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G08-038

    authors: Ebrahimi A,Maury P,Berger M,Kiani SP,Nabipour A,Shariati F,Grieu P,Sarrafi A

    更新日期:2008-08-01 00:00:00

  • Genetic diversity of different apricot geographical groups determined by SSR markers.

    abstract::Forty apricot cultivars with different geographic origins belonging to the germplasm collections of St. Istvan University (Budapest, Hungary) and the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were studied by means of SSR markers. The aim of the study was to determine the genetic relatio...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-128

    authors: Romero C,Pedryc A,Muñoz V,Llácer G,Badenes ML

    更新日期:2003-04-01 00:00:00

  • Chromosome evolution in kangaroos (Marsupialia: Macropodidae): cross species chromosome painting between the tammar wallaby and rock wallaby spp. with the 2n = 22 ancestral macropodid karyotype.

    abstract::Marsupial mammals show extraordinary karyotype stability, with 2n = 14 considered ancestral. However, macropodid marsupials (kangaroos and wallabies) exhibit a considerable variety of karyotypes, with a hypothesised ancestral karyotype of 2n = 22. Speciation and karyotypic diversity in rock wallabies (Petrogale) is ex...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g98-159

    authors: O'Neill RJ,Eldridge MD,Toder R,Ferguson-Smith MA,O'Brien PC,Graves JA

    更新日期:1999-06-01 00:00:00

  • Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled-haploid spring wheat population.

    abstract::Fusarium head blight (FHB) is one of the most important fungal wheat diseases worldwide. Understanding the genetics of FHB resistance is the key to facilitating the introgression of different FHB resistance genes into adapted wheat. The objectives of the present study were to detect and map quantitative trait loci (QT...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-104

    authors: Yang Z,Gilbert J,Fedak G,Somers DJ

    更新日期:2005-04-01 00:00:00

  • 5S rDNA genome regions of Lens species.

    abstract::The length variability of the nontranscribed spacer (NTS) of the 5S rDNA repeats was analyzed in species of the genus Lens by means of PCR amplification. The NTS ranged from approximately 227 to approximately 952 bp. The polymorphism detected was higher than previous NTS polymorphisms described in this genus. Three NT...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-052

    authors: Fernández M,Ruiz ML,Linares C,Fominaya A,Pérez de la Vega M

    更新日期:2005-10-01 00:00:00

  • Genome-wide analysis of the HAK potassium transporter gene family reveals asymmetrical evolution in tobacco (Nicotiana tabacum).

    abstract::Being an essential mineral nutrient, potassium (K+) plays numerous important roles in plant growth and development and determines the yield and quality of crop products. The cellular level of K+ is controlled to a large extent by the K+ transporter, which belongs to the KT/HAK/KUP (HAK) family. However, little is know...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0187

    authors: Song Z,Wu X,Gao Y,Cui X,Jiao F,Chen X,Li Y

    更新日期:2019-04-01 00:00:00

  • Characterisation and physical localisation of Ty1-copia-like retrotransposons in four Alstroemeria species.

    abstract::The genus Alstroemeria contains species with large genomes (2C = 36.5-78.9 pg (17,600-38,000 Mb) in those species with 2n = 2x = 16). We investigated the diversity and genomic and chromosomal organisation of Ty1-copia-like retrotransposons in four Alstroemeria species. Analysis of 33 PCR-amplified sequences correspond...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g98-048

    authors: Kuipers AG,Heslop-Harrison JS,Jacobsen E

    更新日期:1998-06-01 00:00:00

  • Molecular phylogeny of the family of apes and humans.

    abstract::The morphological picture of primate phylogeny has not unambiguously identified the nearest outgroup of Anthropoidea and has not resolved the branching pattern within Hominoidea. The molecular picture provides more resolution and clarifies the systematics of Hominoidea. Protein and DNA evidence divides Hominoidea into...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/g89-050

    authors: Goodman M,Koop BF,Czelusniak J,Fitch DH,Tagle DA,Slightom JL

    更新日期:1989-01-01 00:00:00

  • Development of SSR markers via de novo transcriptome assembly in Akebia trifoliata (Thunb.) Koidz.

    abstract::Owing to its high nutritive, economic, and medicinal values, Akebia trifoliata has received increased attention, making worthy of being used as a new fruit crop for further domestication and commercialization in China. However, molecular research of A. trifoliata has lagged as investigations of its genomic resources a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2019-0068

    authors: Niu J,Wang Y,Shi Y,Wang X,Sun Z,Huang K,Gong C,Luan M,Chen J

    更新日期:2019-12-01 00:00:00

  • Association of the NAD(P)H-bispecific nitrate reductase structural gene with the Nar7 locus in barley.

    abstract::The NADH-specific and NAD(P)H-bispecific nitrate reductase genes from barley have been cloned and sequenced. To determine if the Nar7 locus encodes the NAD(P)H-bispecific nitrate reductase structural gene, a cross was made between a wild-type cultivar, Morex (Nar7 Nar7), and Az70 (nar7w nar7w), a mutant from the culti...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-093

    authors: Warner RL,Kudrna DA,Kleinhofs A

    更新日期:1995-08-01 00:00:00

  • Snapshots of gene expression in rice: limitations for allelic expression imbalance determination.

    abstract::In an initial investigation of differential expression of genes caused by cis-acting regulatory elements in rice, the lack of reproducibility led us to question the basic premise of allelic expression imbalance determination: namely that departures of cDNA expression ratios from those observed in genomic DNA provide u...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g2012-023

    authors: Akkinepalli H,Ereful N,Liu Y,Malabanan K,Howells R,Stamati K,Powell W,Leung H,Greenland A,Mackay I,Lee D

    更新日期:2012-05-01 00:00:00

  • Analysis of codon usage patterns in Morus notabilis based on genome and transcriptome data.

    abstract::Codons play important roles in regulating gene expression levels and mRNA half-lives. However, codon usage and related studies in multicellular organisms still lag far behind those in unicellular organisms. In this study, we describe for the first time genome-wide patterns of codon bias in Morus notabilis (mulberry tr...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0129

    authors: Wen Y,Zou Z,Li H,Xiang Z,He N

    更新日期:2017-06-01 00:00:00

  • Full-length transcriptome analysis and identification of genes involved in asarinin and aristolochic acid biosynthesis in medicinal plant Asarum sieboldii.

    abstract::Asarum sieboldii a well-known traditional Chinese medicinal herb, is used for curing inflammation and ache. It contains both bioactive ingredient asarinin and the toxic compound aristolochic acid. To address further breeding demand, genes involved in the biosynthetic pathways of asarinin and aristolochic acid should b...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0095

    authors: Chen C,Shi X,Zhou T,Li W,Li S,Bai G

    更新日期:2020-12-15 00:00:00

  • Authentication of Ginkgo biloba herbal dietary supplements using DNA barcoding.

    abstract::Ginkgo biloba L. (known as ginkgo or maidenhair tree) is a phylogenetically isolated, charismatic, gymnosperm tree. Herbal dietary supplements, prepared from G. biloba leaves, are consumed to boost cognitive capacity via improved blood perfusion and mitochondrial function. A novel DNA mini-barcode assay was designed a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2014-0130

    authors: Little DP

    更新日期:2014-09-01 00:00:00

  • Detection of cytosine methylation and mapping of a gene influencing cytosine methylation in the genome of Citrus.

    abstract::A new method was developed to detect DNA methylation in the Citrus genome using random amplification coupled with restriction enzyme digestion. Genomic DNA from Citrus grandis (L.) Osb., Poncirus trifoliata (L.) Raf., and their F1 hybrid was amplified using 7 individual 10-mer random primers. Prior to amplification th...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-032

    authors: Cai Q,Guy CL,Moore GA

    更新日期:1996-04-01 00:00:00

  • Genetic mapping of agronomic traits in false flax (Camelina sativa subsp. sativa).

    abstract::The crucifer oilseed plant false flax (Camelina sativa subsp. sativa) possesses numerous valuable agronomic attributes that make it attractive as an alternative spring-sown crop for tight crop rotations. The oil of false flax is particularly rich in polyunsaturated C18-fatty acids, making it a valuable renewable feeds...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-117

    authors: Gehringer A,Friedt W,Lühs W,Snowdon RJ

    更新日期:2006-12-01 00:00:00

  • Arrays of repetitive DNA elements in the largest chromosomes of Toxoplasma gondii.

    abstract::A novel tandemly repeated DNA structure of Toxoplasma gondii that meets the requirements assigned for satellital DNA was characterized. A DNA fragment of 1002 bp contains two different elements of repetitive DNA families named ABGTg7 and ABGTg8.2. Both repeats are members of a more complex tandem structure where ABGTg...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g98-120

    authors: Matrajt M,Angel SO,Pszenny V,Guarnera E,Roos DS,Garberi JC

    更新日期:1999-04-01 00:00:00

  • A genome-wide identification and classification of basic helix-loop-helix genes in the jewel wasp, Nasonia vitripennis (Hymenoptera: Pteromalidae).

    abstract::Basic helix-loop-helix (bHLH) proteins are highly conserved DNA-binding transcription factors of a large superfamily. Animal bHLH proteins play important regulatory roles in various developmental processes such as neurogenesis, myogenesis, heart development, and hematopoiesis. The jewel wasp (Nasonia vitripennis) is a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2014-0171

    authors: Liu XT,Wang Y,Wang XH,Tao XF,Yao Q,Chen KP

    更新日期:2014-10-01 00:00:00

  • Whole genome sequencing of Gir cattle for identifying polymorphisms and loci under selection.

    abstract::Genetic variation in Gir cattle (Bos indicus) has so far not been well characterized. In this study, we used whole genome sequencing of three Gir bulls and a pooled sample from another 11 bulls to identify polymorphisms and loci under selection. A total of 9 990 733 single nucleotide polymorphisms (SNPs) and 604 308 i...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2013-0082

    authors: Liao X,Peng F,Forni S,McLaren D,Plastow G,Stothard P

    更新日期:2013-10-01 00:00:00

  • Relationship between bruchid resistance and seed mass in mungbean based on QTL analysis.

    abstract::Bruchids (Coleoptera: Bruchidae) can cause serious damage to mungbean and several other leguminous crops and there is a strong association between small seed size and bruchid resistance. In investigating the feasibility of breeding large-seeded cultivars with high levels of bruchid resistance, we studied the relations...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G09-031

    authors: Mei L,Cheng XZ,Wang SH,Wang LX,Liu CY,Sun L,Xu N,Humphry ME,Lambrides CJ,Li HB,Liu CJ

    更新日期:2009-07-01 00:00:00

  • Genetic diversity of Crotalaria germplasm assessed through phylogenetic analysis of EST-SSR markers.

    abstract::The genetic diversity of the genus Crotalaria is unknown even though many species in this genus are economically valuable. We report the first study in which polymorphic expressed sequence tag-simple sequence repeat (EST-SSR) markers derived from Medicago and soybean were used to assess the genetic diversity of the Cr...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-027

    authors: Wang ML,Mosjidis JA,Morris JB,Dean RE,Jenkins TM,Pederson GA

    更新日期:2006-06-01 00:00:00

  • Conversion of AFLP markers associated with FHB resistance in wheat into STS markers with an extension-AFLP method.

    abstract::Amplified fragment length polymorphism (AFLP) has proven a powerful tool for tagging genes or quantitative trait loci (QTLs) of interest in plants. However, conversion of AFLP markers into sequence-tagged site (STS) markers is technically challenging in wheat owing to the complicated nature of its genome. In this stud...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-022

    authors: Xu DH,Ban T

    更新日期:2004-08-01 00:00:00

  • Differential susceptibility of a few members of the nasuta-albomicans complex of Drosophila to paraquat-induced lethality and oxidative stress.

    abstract::The evolution of karyotypically stabilized short-lived (SL) and long-lived (LL) cytoraces in the laboratory have been established and validated through our previous lifespan studies. In the present investigation, we examined the possible reason(s) for the differential longevity among selected members of SL and LL cyto...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g11-049

    authors: Ranjini MS,Hosamani R,Muralidhara,Ramachandra NB

    更新日期:2011-10-01 00:00:00

  • Genetic diversity and structure in the northern populations of European hazelnut (Corylus avellana L.).

    abstract::European hazelnut (Corylus avellana L.) is a strictly cross-pollinated diploid tree species, which has its northernmost populations in Fennoscandia, and it was one of the first species to recolonize northern Europe after the last ice age. Hazelnut produces edible nuts in Finland but nowadays they are underutilized as ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0193

    authors: Tanhuanpää P,Heinonen M,Bitz L,Rokka VM

    更新日期:2019-08-01 00:00:00

  • Isolation and preliminary characterization of histone H1.b allelic variants from quail erythrocytes.

    abstract::Our goal was to purify and characterize the allelic variants H1b1 and H1b2 of histone H1.b, one of the seven subtypes of this linker histone extracted from Japanese quail erythrocyte nuclei. These variants are revealed phenotypically as band H1.3 or part of band H1.4 by polyacrylamide gel electrophoresis (PAGE) in sod...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Pałyga J,Neelin JM

    更新日期:1998-10-01 00:00:00

  • Assessment of genetic variation and differentiation of hop genotypes by microsatellite and AFLP markers.

    abstract::Microsatellites have many desirable marker properties and have been increasingly used in crop plants in genetic diversity studies. Here we report on the characterisation of microsatellite markers and on their use for the determination of genetic identities and the assessment of genetic variability among accessions fro...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-44-5-773

    authors: Jakse J,Kindlhofer K,Javornik B

    更新日期:2001-10-01 00:00:00