Cytological and molecular analysis of centromere misdivision in maize.

Abstract:

:B chromosome derivatives suffering from breaks within their centromere were examined cytologically and molecularly. We showed by high resolution FISH that misdivision of the centromere of a univalent chromosome can occur during meiosis. The breaks divide the centromere repeat sequence cluster. A telocentric chromosome formed by misdivision was found to have the addition of telomeric repeats to the broken centromere. A ring chromosome formed after misdivision occurred by fusion of the broken centromere to the telomere. Pulsed-field electrophoresis analyses were performed on the telocentric and ring chromosomes to identify fragments that hybridize to both the telomeric repeat and the B-specific centromeric repeat. We conclude that healing of broken maize centromeres can be achieved through the mechanisms of addition or fusion of telomeric repeat sequences to the broken centromere.

journal_name

Genome

journal_title

Genome

authors

Kaszás E,Kato A,Birchler JA

doi

10.1139/g02-023

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

759-68

issue

4

eissn

0831-2796

issn

1480-3321

journal_volume

45

pub_type

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