Abstract:
:A biochemical marker was utilized to facilitate detection of chromosome 1B, 1BL/1RS translocation heterozygote plants in segregating backcross progenies during the development of 1BL/1RS homozygous lines in several Triticum turgidum L. cultivars (2n = 4x = 28; AABB). Isoelectric focussing of glucose phosphate isomerase (GPI) on either pH 3.5-9.5 or 5.5-8.5 polyacrylamide gels facilitated the detection of 1B, 1BL/1RS translocation heterozygotes from the homozygous 1B or 1BL/1RS derivatives during each backcross of the heterozygote to the respective recurrent parent. The biochemical diagnostic procedure complements the more time consuming and cumbersome chromosome banding technique. This GPI diagnostic in durum 1BL/1RS development is also swifter than a similar stocks development in T. aestivum where both GPI and acid PAGE are essential.
journal_name
Genomejournal_title
Genomeauthors
William MD,Mujeeb-Kazi Adoi
10.1139/g93-144subject
Has Abstractpub_date
1993-12-01 00:00:00pages
1088-91issue
6eissn
0831-2796issn
1480-3321pii
g93-144journal_volume
36pub_type
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