Draft genome sequence, disease-resistance genes, and phenotype of a Paenibacillus terrae strain (NK3-4) with the potential to control plant diseases.

Abstract:

:Paenibacillus terrae NK3-4 is a plant growth-promoting rhizobacterium that may be useful for controlling plant diseases. We conducted a genomic analysis and identified the genes mediating antimicrobial functions. Additionally, an extracellular antifungal protein component was isolated and identified. The draft genome sequence was assembled into 54 contigs, with 5 458 568 bp and a G+C content of 47%. Moreover, 4 690 015 bp encoded 5090 proteins, 7 rRNAs, and 54 tRNAs. Forty-four genes involved in antimicrobial functions were detected. They mainly encode 19 non-ribosomal peptide synthetases (NRPSs); one polyketide synthase/NRPSs hybrid enzyme; four Zn-dependent metalloproteases; three antilisterial bacteriocin subtilosin biosynthesis proteins (AlbA); four serine proteases; five pectate lyases; three beta-glucanases; and four 1,4-beta-xylanases. These include four novel NRPSs that have not been found in any species of Paenibacillus. Furthermore, five proteins exhibiting antifungal activity were identified from the antifungal extracellular protein component based on MS/MS and the strain NK3-4 predicted protein library. On the basis of these features, we propose that strain NK3-4 represents a promising biocontrol agent for protecting plant from diseases. The draft genome sequence described herein may provide the genetic basis for the characterization of the molecular mechanisms underlying the biocontrol functions. It may also facilitate the development of rational strategies for improving the strain.

journal_name

Genome

journal_title

Genome

authors

Yu WQ,Zheng GP,Qiu W,Yan FC,Liu WZ,Liu WX

doi

10.1139/gen-2018-0113

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

725-734

issue

10

eissn

0831-2796

issn

1480-3321

journal_volume

61

pub_type

杂志文章

相关文献

GENOME文献大全
  • Insect mitochondrial genomics 2: The complete mitochondrial genome sequence of a giant stonefly, Pteronarcys princeps, asymmetric directional mutation bias, and conserved plecopteran A+T-region elements.

    abstract::Mitochondrial (mt) genome sequences of insects are receiving renewed attention in molecular phylogentic studies, studies of mt-genome rearrangement, and other unusual molecular phenomena, such as translational frameshifting. At present, the basal neopteran lineages are poorly represented by mt-genome sequences. Comple...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-037

    authors: Stewart JB,Beckenbach AT

    更新日期:2006-07-01 00:00:00

  • Phylogeny and expression of paralogous and orthologous sulphate transporter genes in diploid and hexaploid wheats.

    abstract::Twelve genes encoding two closely related subtypes (ST1.1a and ST1.1b) of a sulphate transporter have been identified in the diploid wheats Aegilops tauschii, Triticum urartu, and Aegilops speltoides, as well as the hexaploid Triticum aestivum. Based on phylogenetic comparisons with other plant sulphate transporters, ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-011

    authors: Buchner P,Prosser IM,Hawkesford MJ

    更新日期:2004-06-01 00:00:00

  • Spectral karyotyping (SKY) of mouse meiotic chromosomes.

    abstract::The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalents can be identifie...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g01-018

    authors: Heng HH,Liu G,Lu W,Bremer S,Ye CJ,Hughes M,Moens P

    更新日期:2001-04-01 00:00:00

  • Genetic diversity within the species Arachis duranensis Krapov. &W.C. Gregory, a possible progenitor of cultivated peanut.

    abstract::Eighteen accessions of a diploid wild peanut species (Arachis duranensis) were analyzed using morphological, intercrossing, cytological, and RFLP data. Abundant variation was found for morphological characters and for RFLP patterns both between and within accessions, and each accession could be uniquely identified by ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-158

    authors: Stalker HT,Dhesi JS,Kochert G

    更新日期:1995-12-01 00:00:00

  • Development of a selection index to improve market value of cultured Atlantic salmon (Salmo salar).

    abstract::Genetic parameters were estimated on growth and development traits using analyses of variance and covariance of 42 full-sib families from a select line, 39 full-sib families from a control line of the same strain, and 37 full-sib families from another unselected strain. The traits included percent 1+ smolts, percent s...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g92-046

    authors: O'Flynn FM,Friars GW,Bailey JK,Terhune JM

    更新日期:1992-04-01 00:00:00

  • Evolutionary distribution of light-harvesting complex-like proteins in photosynthetic eukaryotes.

    abstract::Light-harvesting-like (LIL) proteins are low-molecular-mass membrane proteins related to the light-harvesting complexes, which form the dominant antenna system in most photosynthetic eukaryotes. To analyze the LIL protein family, we mined a number of publicly available databases to identify members of this family in a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g09-081

    authors: Neilson JA,Durnford DG

    更新日期:2010-01-01 00:00:00

  • Study on correlation of signal molecule genes and their receptor-associated genes with rat liver regeneration.

    abstract::To investigate the effect of signal molecules and their receptor-associated genes on rat liver regeneration (LR) at the transcriptional level, the associated genes were originally obtained by retrieving the databases and related scientific publications; their expression profiles in rat LR were then checked using the R...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g09-022

    authors: Xu CS,Shao HY,Du B

    更新日期:2009-06-01 00:00:00

  • Gene flow, recombination, and positive selection in Stenotrophomonas maltophilia: mechanisms underlying the diversity of the widespread opportunistic pathogen.

    abstract::Stenotrophomonas maltophilia is a global multidrug-resistant human opportunistic pathogen in clinical environments. Stenotrophomonas maltophilia is also ubiquitous in aqueous environments, soil, and plants. Various molecular typing methods have revealed that S. maltophilia exhibits high levels of phenotypic and genoty...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0073

    authors: Yu D,Yin Z,Li B,Jin Y,Ren H,Zhou J,Zhou W,Liang L,Yue J

    更新日期:2016-12-01 00:00:00

  • Chromosomal location of three wheat sequences with homology to pollen allergen encoding, DNA replication regulating, and DNA (cytosine-5)-methyltransferase genes in wheat and rye.

    abstract::Three wheat sequences, shown to be homologous to pollen allergen encoding, DNA replication regulating, and DNA (cytosine-5)-methyltransferase genes were localized on chromosomes using nullisomic-tetrasomic wheat ('Chinese Spring') and wheat-rye ('Chinese Spring'/'Imperial') addition lines. Whereas the loci for the pol...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-154

    authors: Korzun V,Balzer HJ,Balzer A,Bäumlein H,Börner A

    更新日期:1996-12-01 00:00:00

  • The genetic identity of alien chromosomes in potato breeding lines revealed by sequential GISH and FISH analyses using chromosome-specific cytogenetic DNA markers.

    abstract::Genomic in situ hybridization (GISH) is one of the most popular and effective techniques for detecting alien chromatin introgressed into breeding lines; however, GISH analysis alone does not reveal the genetic identity of the alien chromosomes. We previously isolated a set of bacterial artificial chromosomes (BACs) sp...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Dong F,McGrath JM,Helgeson JP,Jiang J

    更新日期:2001-08-01 00:00:00

  • QTL mapping of BNYVV resistance from the WB41 source in sugar beet.

    abstract::The most important rhizomania-resistance gene in sugar beet is the Rz1 gene from the Holly Sugar Company in California, the source widely used to breed partially resistant varieties. Other important gene sources are WB41 and WB42, which both originate from Beta vulgaris subsp. maritima collected in Denmark, and which ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-108

    authors: Gidner S,Lennefors BL,Nilsson NO,Bensefelt J,Johansson E,Gyllenspetz U,Kraft T

    更新日期:2005-04-01 00:00:00

  • Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas.

    abstract::Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromos...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-087

    authors: Bouilly K,Leitão A,Chaves R,Guedes-Pinto H,Boudry P,Lapègue S

    更新日期:2005-02-01 00:00:00

  • 5S rDNA genome regions of Lens species.

    abstract::The length variability of the nontranscribed spacer (NTS) of the 5S rDNA repeats was analyzed in species of the genus Lens by means of PCR amplification. The NTS ranged from approximately 227 to approximately 952 bp. The polymorphism detected was higher than previous NTS polymorphisms described in this genus. Three NT...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-052

    authors: Fernández M,Ruiz ML,Linares C,Fominaya A,Pérez de la Vega M

    更新日期:2005-10-01 00:00:00

  • Large-scale development of functional markers in Brassica species.

    abstract::Numerous quantitative trait loci (QTL) have been detected in Brassica species, but fine-mapping of major QTL has advanced slowly. The development of functional markers can overcome this barrier. We used publicly available PlantGDB-assembled unique transcripts (PUTs) from Brassica species to design 7836 functional simp...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g11-042

    authors: An Z,Gao C,Li J,Fu D,Tang Z,Ortegón O

    更新日期:2011-09-01 00:00:00

  • High-density SNP mapping reveals closely linked QTL for resistance to Stagonospora nodorum blotch (SNB) in flag leaf and glume of hexaploid wheat.

    abstract::The genetic control of adult plant resistance to Stagonospora nodorum blotch (SNB) is complex, consisting of genes with minor effects interacting in an additive manner. Earlier studies detected quantitative trait loci (QTL) for flag leaf resistance in successive years on chromosomes 1B, 2A, 2D, and 5B using SSR- and D...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2017-0203

    authors: Francki MG,Walker E,Li DA,Forrest K

    更新日期:2018-02-01 00:00:00

  • Molecular mapping of resistance to Leptosphaeria maculans in Australian cultivars of Brassica napus.

    abstract::Doubled haploid (DH) lines together with a cotyledon bioassay were employed for the molecular analysis of resistance to the blackleg fungus Leptosphaeria maculans in the Australian Brassica napus cultivars Shiralee and Maluka. We used bulked segregant analysis to identify 13 RAPD and two RFLP markers linked to the res...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g97-041

    authors: Mayerhofer R,Good AG,Bansal VK,Thiagarajah MR,Stringam GR

    更新日期:1997-06-01 00:00:00

  • Snapshots of gene expression in rice: limitations for allelic expression imbalance determination.

    abstract::In an initial investigation of differential expression of genes caused by cis-acting regulatory elements in rice, the lack of reproducibility led us to question the basic premise of allelic expression imbalance determination: namely that departures of cDNA expression ratios from those observed in genomic DNA provide u...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g2012-023

    authors: Akkinepalli H,Ereful N,Liu Y,Malabanan K,Howells R,Stamati K,Powell W,Leung H,Greenland A,Mackay I,Lee D

    更新日期:2012-05-01 00:00:00

  • Genome evolution of intermediate wheatgrass as revealed by EST-SSR markers developed from its three progenitor diploid species.

    abstract::Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey), a segmental autoallohexaploid (2n = 6x = 42), is not only an important forage crop but also a valuable gene reservoir for wheat (Triticum aestivum L.) improvement. Throughout the scientific literature, there continues to be disagreement as...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2014-0186

    authors: Wang RR,Larson SR,Jensen KB,Bushman BS,DeHaan LR,Wang S,Yan X

    更新日期:2015-02-01 00:00:00

  • Bacterial diversity in the waterholes of the Kruger National Park: an eDNA metabarcoding approach 1.

    abstract::Bacteria are essential components of natural environments. They contribute to ecosystem functioning through roles as mutualists and pathogens for larger species, and as key components of food webs and nutrient cycles. Bacterial communities respond to environmental disturbances, and the tracking of these communities ac...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0064

    authors: Farrell MJ,Govender D,Hajibabaei M,van der Bank M,Davies TJ

    更新日期:2019-03-01 00:00:00

  • Phaseolin nucleotide sequence diversity in Phaseolus. I. Intraspecific diversity in Phaseolus vulgaris.

    abstract::Most information about the molecular biology of phaseolin, the major seed storage protein in Phaseolus vulgaris, has been obtained from the T-type phaseolin, which is characteristic of the Andean gene pool of the species. In the work reported here, two cDNA clones for the S-type phaseolin representing the other major,...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-107

    authors: Kami JA,Gepts P

    更新日期:1994-10-01 00:00:00

  • Karyotype evolution in the Pinaceae: implication with molecular phylogeny.

    abstract::The family Pinaceae is made up mostly of diploid species (2n = 24). Systematization of karyotype analysis was developed to make comparison of intra- and interspecific karyotypes among the Pinaceae more accurate and reliable. Considering all parameters, the genera Pseudotsuga and Pseudolarix have the "most derived" (or...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/g2012-061

    authors: Nkongolo KK,Mehes-Smith M

    更新日期:2012-11-01 00:00:00

  • Spontaneous structural rearrangements in Solanum phureja Juz. et Buk. 2. Meiotic behaviour and identification of interchange chromosomes using primary trisomics.

    abstract::Meiosis was studied in two diploid (2n = 2x = 24) siblings of Solanum phureja Juz. et Buk. and in 11 disomic and 2 trisomic descendants. The diploid siblings carry the same heterozygous interchange and either one or two inversions. The frequency of quadrivalents at diakinesis/metaphase I in these clones was 0.56 and 0...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-017

    authors: Wagenvoort M

    更新日期:1995-02-01 00:00:00

  • Linkage and mapping analyses of the densonucleosis non-susceptible gene nsd-Z in the silkworm Bombyx mori using SSR markers.

    abstract::In the silkworm Bombyx mori, non-susceptibility to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is located on chromosome 15. Owing to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progeny were used for...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-125

    authors: Li M,Guo Q,Hou C,Miao X,Xu A,Guo X,Huang Y

    更新日期:2006-04-01 00:00:00

  • Analysis of 106 kb of contiguous DNA sequence from the D genome of wheat reveals high gene density and a complex arrangement of genes related to disease resistance.

    abstract::Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-049

    authors: Brooks SA,Huang L,Gill BS,Fellers JP

    更新日期:2002-10-01 00:00:00

  • Genomic in situ hybridization analysis of Thinopyrum chromatin in a wheat-Th. intermedium partial amphiploid and six derived chromosome addition lines

    abstract::The genomic origin of alien chromosomes present in a wheat-Thinopyrum intermedium partial amphiploid TAF46 (2n = 8x = 56) and six derived chromosome addition lines were analyzed by genomic in situ hybridization (GISH) using S genomic DNA from Pseudoroegneria strigosa (2n = 2x = 14, SS) as a probe. The GISH analysis cl...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g99-090

    authors: Chen Q,Conner RL,Laroche A,Ji WQ,Armstrong KC,Fedak G

    更新日期:1999-12-01 00:00:00

  • New porcine microRNA genes found by homology search.

    abstract::MicroRNAs (miRNAs) repress target genes at the post-transcriptional level and play important roles in development and cell lineage decisions. In vertebrates, however, both the targets of miRNAs and their expression profiles during development are poorly understood. Thus far, 326 human miRNAs, 249 mouse miRNAs, and 195...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-120

    authors: Kim HJ,Cui XS,Kim EJ,Kim WJ,Kim NH

    更新日期:2006-10-01 00:00:00

  • Sequence analysis of single-copy genes in two wild olive subspecies: nucleotide diversity and potential use for testing admixture.

    abstract::The wild olive distribution extends from the Mediterranean region to south Asia and Austral Africa. The species is also invasive, particularly in Australia. Here, we investigated the sequence variation at five nuclear single-copy genes in 41 native and invasive accessions of the Mediterranean and African olive subspec...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2014-0001

    authors: Besnard G,El Bakkali A

    更新日期:2014-03-01 00:00:00

  • Detection of large sequence insertions by a hybrid approach that combine de novo assembly and resequencing of medium-coverage genome sequences.

    abstract::Large sequence insertion (LSI) is one of the structural variations (SVs) that may cause phenotypic differences in plants. To identify the LSIs using medium-coverage sequencing data of four wild soybean (Glycine soja) genotypes, we designed a hybrid approach combining de novo assembly and read mapping. Total reads and ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0027

    authors: Lee T,Kim MY,Ha J,Lee SH

    更新日期:2018-10-01 00:00:00

  • Genetic diversity analysis in blackgram (Vigna mungo (L.) Hepper) using AFLP and transferable microsatellite markers from azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi).

    abstract::Genetic diversity in 20 elite blackgram (Vigna mungo (L.) Hepper) genotypes was studied using microsatellite and AFLP markers. Thirty-six microsatellite markers from azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) were successfully amplified across the 20 blackgram genotypes and 33 microsatellite markers showed po...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g08-107

    authors: Gupta SK,Gopalakrishna T

    更新日期:2009-02-01 00:00:00

  • Organization of 5S ribosomal RNA genes in tea (Camellia sinensis).

    abstract::The 5S rRNA genes in the Camellia sinensis (L.) O. Kuntze (tea) genome are arranged as tandem repeat units of 300 and 325 bps. The 2 classes of tandem repeats were discovered by Southern hybridisation of tea genomic DNA with a 5S rRNA gene PCR product. ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-44-1-143

    authors: Singh D,Singh M

    更新日期:2001-02-01 00:00:00