Abstract:
:Restriction endonuclease TaqI has been known as a nonbanding restriction endonuclease when it is used on fixed human chromosomes. However, a specific TaqI digestion can be obtained after varying experimental conditions such as concentration of enzyme, time of incubation, and volume of the final reaction mixture. This digestion consists of an extensive DNA loss in heterochromatin subregions of chromosomes 1, 9, 15, 16, and Y. These regions essentially coincide with those corresponding to the main chromosome locations of satellite II DNA, whose tandem repeated units contain many TaqI target sequences, and some satellite III DNA domains enriched in TaqI sites.
journal_name
Genomejournal_title
Genomeauthors
Tagarro I,González-Aguilera JJ,Fernández-Peralta AMdoi
10.1139/g91-039subject
Has Abstractpub_date
1991-04-01 00:00:00pages
251-4issue
2eissn
0831-2796issn
1480-3321journal_volume
34pub_type
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