TaqI digestion reveals fractions of satellite DNAs on human chromosomes.

Abstract:

:Restriction endonuclease TaqI has been known as a nonbanding restriction endonuclease when it is used on fixed human chromosomes. However, a specific TaqI digestion can be obtained after varying experimental conditions such as concentration of enzyme, time of incubation, and volume of the final reaction mixture. This digestion consists of an extensive DNA loss in heterochromatin subregions of chromosomes 1, 9, 15, 16, and Y. These regions essentially coincide with those corresponding to the main chromosome locations of satellite II DNA, whose tandem repeated units contain many TaqI target sequences, and some satellite III DNA domains enriched in TaqI sites.

journal_name

Genome

journal_title

Genome

authors

Tagarro I,González-Aguilera JJ,Fernández-Peralta AM

doi

10.1139/g91-039

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

251-4

issue

2

eissn

0831-2796

issn

1480-3321

journal_volume

34

pub_type

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