Low-copy microsatellite markers for Pinus taeda L.

Abstract:

:Eighteen low-copy and genomic microsatellite markers were tested for Mendelian inheritance and then assayed in 41 Pinus taeda L. samples drawn from five regions in the southern United States. The PCR products had multiple alleles, high levels of polymorphism, and little non-specific priming. Fifteen of the 18 markers were informative for a P. taeda three-generation RFLP (restriction fragment length polymorphism) pedigree, and a P. taeda population survey revealed three to 28 alleles per locus. The highest allele numbers and polymorphic information content (PIC) values were associated with complex repeat sequences and (or) with sequences consisting of the longer strings of perfect repeats. The abundance of low- to rare-frequency alleles also accounted for high PIC values in both types of markers. Low-copy microsatellites are useful for the large, complex pine genome, especially in the absence of entire gene sequences in public databases and with the low levels of polymorphism in markers developed from expressed sequence tags (ESTs).

journal_name

Genome

journal_title

Genome

authors

Elsik CG,Minihan VT,Hall SE,Scarpa AM,Williams CG

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

550-5

issue

3

eissn

0831-2796

issn

1480-3321

journal_volume

43

pub_type

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