Analysis of Brachypodium genomes with genome-wide optical maps.

Abstract:

:Brachypodium distachyon (n = 5) is a diploid and has been widely used as a genetic model. Brachypodium stacei (n = 10) and B. hybridum (n = 15) are species that are related to B. distachyon, leading to an hypothesis that they are part of a polyploid series based on x = 5. Several lines of evidence suggest that this hypothesis is incorrect and that the genomes of the three taxa may have evolved by a more complex process. We constructed an optical whole-genome BioNano genome (BNG) map for each species and did pairwise alignment of the BNG maps. The maps showed that B. distachyon and B. stacei are both diploid, in spite of B. stacei having twice as many chromosomes as B. distachyon, and that B. hybridum is an allopolyploid formed from hybridization between B. distachyon and B. stacei. This study also demonstrated the use of BNG maps in the detection and quantification of structural variants among the genomes.

journal_name

Genome

journal_title

Genome

authors

Zhu T,Hu Z,Rodriguez JC,Deal KR,Dvorak J,Vogel JP,Liu Z,Luo MC

doi

10.1139/gen-2018-0013

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

559-565

issue

8

eissn

0831-2796

issn

1480-3321

journal_volume

61

pub_type

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