Characterization and mapping of a male-sterility mutant, tapetum desquamation (t), in rice.

Abstract:

:A spontaneously mutated male-sterile material was found among the offspring of the indica restorer line Jinhuiyihao. To understand the status and function of the related gene and clone the gene, a near-isogenic line (NIL) of the male sterility was bred, and characterization of the mutant and gene mapping were performed. The results indicated that there are obvious differences between the male-sterile NIL and the indica maintainer line II-32B. The anther size of the NIL is smaller than that of II-32B, and the anther color is white in the NIL but yellow in II-32B. No pollen from the matured anther in the NIL was observed to be stained using KI-I2 solution. In transverse sections of the sterile anther, at early microspore stage the cytoplasm of the tapetum concentrates but the tapetum itself does not degenerate after microspores are released from the tetrads; the tapetum then desquamates from the anther wall and enwraps microspores; subsequently, the surrounded microspores collapse completely at late microspore and early bicellular pollen stages. Inheritance analysis showed that the male sterility was controlled by a single recessive gene, ostd (t). This gene was mapped between the SSR markers RM7434 and RM275 on chromosome 6, and the physical distance from RM7434 to RM275 is about 389 kb.

journal_name

Genome

journal_title

Genome

authors

Zhang Y,Mao JX,Yang K,Li YF,Zhang J,Huang YX,Shen FC,Zhang CD

doi

10.1139/G08-013

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

368-74

issue

5

eissn

0831-2796

issn

1480-3321

pii

g08-013

journal_volume

51

pub_type

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