Substitution pattern of the CArG element in human and mouse genomes.

Abstract:

:cis-Elements CArG bound by serum response factor (SRF) are presently being intensively studied, but little is known about the substitution pattern of functional CArG elements. Here, we have performed the first evolutionary analysis of CArGome in the human and mouse genome through bioinformatic methods and statistical tests. We calculated the substitution rate at each site of the functional CArG elements. The results showed that the core sites of the functional CArG elements evolved faster than did the background DNA, indicating that these sites were likely to evolve under positive selection. Moreover, a strong TATA "motif" was evident in the core region within the functional CArG elements in both human and mouse promoters. This motif could probably be a major contribution to the formation of the spatial structure, which was important for CArG-SRF recognition. Thus, the study further revealed the sequence character and substitution pattern of CArG elements and provided useful information for the study of the SRF-binding efficiencies of CArG promoters in functional assays.

journal_name

Genome

journal_title

Genome

authors

Shen X,Mao H,Miao S

doi

10.1139/G10-105

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

144-50

issue

2

eissn

0831-2796

issn

1480-3321

pii

g10-105

journal_volume

54

pub_type

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