Abstract:
:The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines was subjected to RAPD screening with 36 random decamer arbitrary primers. A total of 241 scorable products were observed with 54 (22%) being polymorphic. The RAPD data showed that the parental lines of this commercial cabbage cultivar were not very closely related. Two primers were chosen for purity testing of the F1-hybrid seeds. The sib (inbred seed; seed from self-pollination of parental lines) contamination results obtained by RAPD analysis were comparable to the commonly used grow-out trial and isozyme analysis, hence showing that RAPD analysis can be used for seed purity testing of commercial hybrid cabbage seeds.
journal_name
Genomejournal_title
Genomeauthors
Crockett PA,Bhalla PL,Lee CK,Singh MBsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
317-21issue
2eissn
0831-2796issn
1480-3321journal_volume
43pub_type
杂志文章相关文献
GENOME文献大全abstract::Knowledge of the locations of repeat elements could be very important in the assembly of genome sequences and their assignment to physical chromosomes. Genomic and species relationships among 16 species were investigated using fluorescence in situ hybridization (FISH) with the Am1 and (ACT)6 probes. The Am1 oligonucle...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0132
更新日期:2018-01-01 00:00:00
abstract::B chromosome derivatives suffering from breaks within their centromere were examined cytologically and molecularly. We showed by high resolution FISH that misdivision of the centromere of a univalent chromosome can occur during meiosis. The breaks divide the centromere repeat sequence cluster. A telocentric chromosome...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-023
更新日期:2002-08-01 00:00:00
abstract::The establishment of a C-banded karyotype of hexaploid oat (Avena spp., 2n = 6x = 42) has facilitated the cytological characterization of a monosomic series in 'Kanota', an A. byzantina (C. Koch) cultivar. The 'Kanota' series of monosomics analyzed in this study consists of only 12 of the 21 different chromosome-defic...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-126
更新日期:1993-10-01 00:00:00
abstract::Mutations in High temperature requirement A2 (HtrA2), also designated PARK13, which lead to the loss of its protease activity, have been associated with Parkinson's disease (PD). HtrA2 is a mitochondrial protease that translocates to the cytosol upon the initiation of apoptosis where it participates in the abrogation ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2016-0069
更新日期:2017-01-01 00:00:00
abstract::The brown frog Rana graeca was believed to be present in two areas, the Balkan Peninsula and the Italian Apennines. We have characterised the S1 satellite DNA family from Rana graeca graeca and compared it with that of Rana graeca italica. On Southern blots, the patterns of S1 satellite DNA bands are very different be...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-125
更新日期:2002-02-01 00:00:00
abstract::Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey), a segmental autoallohexaploid (2n = 6x = 42), is not only an important forage crop but also a valuable gene reservoir for wheat (Triticum aestivum L.) improvement. Throughout the scientific literature, there continues to be disagreement as...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2014-0186
更新日期:2015-02-01 00:00:00
abstract::The combination of two divergent genomes during hybridization can result in "genome shock". Although genome shock has been reported in the hybrids of some herbaceous plants, the pattern and the principle it follows are far from understood, especially in woody plants. Here, the gene expression patterns were remodeled i...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0105
更新日期:2018-07-01 00:00:00
abstract::The genetic control of adult plant resistance to Stagonospora nodorum blotch (SNB) is complex, consisting of genes with minor effects interacting in an additive manner. Earlier studies detected quantitative trait loci (QTL) for flag leaf resistance in successive years on chromosomes 1B, 2A, 2D, and 5B using SSR- and D...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0203
更新日期:2018-02-01 00:00:00
abstract::Using contiguous genomic DNA sequences of Arabidopsis thaliana, we were able to identify a region of conserved structure in the genome of rice. The conserved, and presumptive homoeologous segments, are 194 kb and 219-300 kb in size in Arabidopsis and rice, respectively. They contain five homologous genes, distinguishe...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::The Mpv17l protein has two isoforms, M-LPL and M-LPS, which both regulate the production of reactive oxygen species (ROS) and protect against mitochondrial oxidative stress and apoptosis. M-LPL is ubiquitously expressed, while M-LPS is expressed mostly in the kidney of aged animals. We identified a variety of transcri...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g09-061
更新日期:2009-11-01 00:00:00
abstract::Two nuclear genes, vitality (Vi) on an A- or B-genome chromosome and species cytoplasm specific (scs) on a 1DL telosome from Triticum aestivum L. or a telosome from Aegilops uniaristata Vis. (un telosome), improved compatibility between the nucleus of Triticum turgidum L. var. durum and the cytoplasm of Ae. longissima...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-029
更新日期:1994-04-01 00:00:00
abstract::Plant disease resistance gene analog (RGA) markers were designed according to the conserved sequence of known RGAs and used to map resistance genes. We used genome-wide RGA markers for genetic analyses of structure and diversity in a global rice germplasm collection. Of the 472 RGA markers, 138 were polymorphic and th...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2012-0142
更新日期:2013-07-01 00:00:00
abstract::Segregation patterns of polymorphic simple sequence repeat (SSR) primer pairs were investigated in monoploid potato families derived from anther culture. A total of 14 primers developed from the sequences in the database, as well as from a genomic library of potato, was used. Distorted segregation was observed for sev...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-140
更新日期:2002-04-01 00:00:00
abstract::A survey of restriction fragment polymorphism in mitochondrial DNA (mtDNA) of 17 populations of the mosquito Aedes albopictus was undertaken. The mtDNA size was estimated to be about 17.5 kbp. The level of polymorphism was low, with over 99% of the fragments being shared in common among the 17 populations. Three popul...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g91-046
更新日期:1991-04-01 00:00:00
abstract::Disease resistance gene candidates (RGCs) belonging to the nucleotide-binding site (NBS) superfamily have been cloned from numerous crop plants using highly conserved DNA sequence motifs. The aims of this research were to (i) isolate genomic DNA clones for RGCs in cultivated sunflower (Helianthus annuus L.) and (ii) m...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-110
更新日期:2001-04-01 00:00:00
abstract::The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reac...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-034
更新日期:1996-04-01 00:00:00
abstract::Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify ...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::Despite being a controversial crop, Cannabis sativa L. has a long history of cultivation throughout the world. Following recent legalisation in Canada, it is emerging as an important plant for both medicinal and recreational purposes. Recent progress in genome sequencing of both cannabis and hemp varieties allows for ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2020-0087
更新日期:2020-11-13 00:00:00
abstract::The wild olive distribution extends from the Mediterranean region to south Asia and Austral Africa. The species is also invasive, particularly in Australia. Here, we investigated the sequence variation at five nuclear single-copy genes in 41 native and invasive accessions of the Mediterranean and African olive subspec...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2014-0001
更新日期:2014-03-01 00:00:00
abstract::Multiple-target fluorescence in situ hybridization (FISH) was applied on mitotic chromosomes of seven Lens taxa using two highly repetitive sequences (pLc30 and pLc7) isolated from the cultivated lentil and the multigene families for the 18S-5.8S-25S (pTa71) and 5S rRNA (pTa794) from wheat simultaneously as probes. Th...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-077
更新日期:2003-12-01 00:00:00
abstract::The NADH-specific and NAD(P)H-bispecific nitrate reductase genes from barley have been cloned and sequenced. To determine if the Nar7 locus encodes the NAD(P)H-bispecific nitrate reductase structural gene, a cross was made between a wild-type cultivar, Morex (Nar7 Nar7), and Az70 (nar7w nar7w), a mutant from the culti...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-093
更新日期:1995-08-01 00:00:00
abstract::Abstract: Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males highlighted the W chromosomes in mitotic plates and the W chromatin in polyploid interphase nuclei of the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-003
更新日期:2003-04-01 00:00:00
abstract::Polymorphic expressed sequence tag - simple sequence repeat (EST-SSR) markers derived from major cereal crops were used to assess the genetic diversity of the USDA temperate bamboo collection consisting of 92 accessions classified in 11 separate genera and 44 species. A total of 211 bands were detected with a mean num...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-022
更新日期:2005-08-01 00:00:00
abstract::European hazelnut (Corylus avellana L.) is a strictly cross-pollinated diploid tree species, which has its northernmost populations in Fennoscandia, and it was one of the first species to recolonize northern Europe after the last ice age. Hazelnut produces edible nuts in Finland but nowadays they are underutilized as ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2018-0193
更新日期:2019-08-01 00:00:00
abstract::Among 15 wild perennial species of the genus Glycine Willd. subgenus Glycine, G. tomentella is exceptional. It is composed of four cytotypes (2n = 38, 40, 78, 80), is diverse in morphological features, and covers a wide geographical area. The objectives of this study were to uncover the genomic diversity in 78- and 80...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-063
更新日期:1994-06-01 00:00:00
abstract::Copines are evolutionarily conserved calcium-dependent membrane-binding proteins with potentially critical biological functions. In plants, the function of these proteins has not been analyzed except for in Arabidopsis thaliana where they play critical roles in development and disease resistance. To facilitate functio...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2016-0015
更新日期:2016-08-01 00:00:00
abstract::Despite the recent advances in plant population genetic studies, the lack of information regarding pedigree, ploidy level, or mode of inheritance for many polyploids can compromise the analysis of the molecular data produced. The aim of this study was to examine both microsatellite and cytogenetic characteristics of t...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2013-0159
更新日期:2013-12-01 00:00:00
abstract::A genetic linkage map of Brassica rapa L. was constructed using recombinant inbred lines (RILs) derived from a cross between yellow-seeded cultivar Sampad and a yellowish brown seeded inbred line 3-0026.027. The RILs were evaluated for seed color under three conditions: field plot, greenhouse, and controlled growth ch...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-066
更新日期:2012-12-01 00:00:00
abstract::The aim of this study was to investigate the differential expression characteristics and the roles of the genome-wide microRNAs (miRNAs) in immunoglobulin A nephropathy (IgAN) kidney tissues. We used Illumina high-throughput sequencing technology to evaluate the miRNAs expression of six biopsy tissues from IgAN and si...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2012-0159
更新日期:2013-03-01 00:00:00
abstract::A new repetitive sequence that is extremely abundant and well dispersed in the Thinopyrum elongatum genome but present in low-copy number in wheat (Triticum aestivum L.) has been isolated. This repeat and a Th. elongatum repeat isolated in another laboratory were used to identify cosmid genomic clones containing the r...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-012
更新日期:1994-02-01 00:00:00