Abstract:
:Knowledge of the locations of repeat elements could be very important in the assembly of genome sequences and their assignment to physical chromosomes. Genomic and species relationships among 16 species were investigated using fluorescence in situ hybridization (FISH) with the Am1 and (ACT)6 probes. The Am1 oligonucleotide probe was particularly enriched in the C genomes, whereas the (ACT)6 trinucleotide repeat probe showed a diverse distribution of hybridization patterns in the A, AB, C, AC, and ACD genomes but might not be present in the B and D genomes. The hybridization pattern of Avena sativa was very similar to that of A. insularis, indicating that this species most likely originated from A. insularis as a tetraploid ancestor. Although the two FISH probes failed to identify relationships of more species, this proof-of-concept approach opens the way to the use of FISH probes in assigning other signature elements from genomic sequence to physical chromosomes.
journal_name
Genomejournal_title
Genomeauthors
Luo X,Tinker NA,Zhou Y,Wight CP,Liu J,Wan W,Chen L,Peng Ydoi
10.1139/gen-2017-0132subject
Has Abstractpub_date
2018-01-01 00:00:00pages
63-70issue
1eissn
0831-2796issn
1480-3321journal_volume
61pub_type
杂志文章相关文献
GENOME文献大全abstract::Resistance to common root rot and black point, caused by Cochliobolus sativus, was evaluated in alien chromosome substitution and addition lines of the cultivars 'Cadet' and 'Rescue'. Substitution of chromosome 5B in 'Rescue' with 5Ag from Agropyron elongatum decreased root rot susceptibility to a level intermediate b...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-023
更新日期:1993-02-01 00:00:00
abstract::Five wheat-barley translocations in a wheat background were characterized through the combination of cytogenetic and molecular genetic approaches. The wheat chromosome segments involved in the translocations were identified using sequential GISH and two-colour FISH with the probes pSc119.2 and pAs1. The barley chromat...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-068
更新日期:2002-12-01 00:00:00
abstract::The purposes of the present research are (i) to establish the karyotype of Neursopora crassa using visualization of kinetochores in the synaptonemal complex (SC) spreads, (ii) to assign each chromosome to a linkage group, and (iii) to examine chromosome pairing and recombination nodules in quadrivalents. Two strains c...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-008
更新日期:2006-06-01 00:00:00
abstract::Multiple reaction monitoring mass spectrometry (MRM-MS) is an emerging technology for blood biomarker verification and validation; however, the results may be influenced by pre-analytical factors. This exploratory study was designed to determine if differences in phlebotomy techniques would significantly affect the ab...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0036
更新日期:2015-12-01 00:00:00
abstract::Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-049
更新日期:2002-10-01 00:00:00
abstract::Complex chromosome rearrangements (CCRs) are defined as structural abnormalities involving more than two chromosome breaks, coupled with exchanges of chromosomal segments. Information on CCRs in plants is limited. In the present study, a plant (26-4) harboring translocation chromosomes 1RS.1BL and 4RS.4DL was selected...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2016-0112
更新日期:2016-12-01 00:00:00
abstract::Changes in chromosome number have played an important role in the evolution of the genus Trifolium. Along with a few species of polyploid origin there are several cases of dysploidy as evidenced by the presence of four basic chromosome numbers (x = 8, 7, 6, 5). Trifolium subterraneum and Trifolium israeliticum are rel...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2013-0055
更新日期:2013-06-01 00:00:00
abstract::The standard karyotype and a wide array of repatterned cytotypes from 21 demes of the double-allotriploid thelytokous Bacillus lynceorum have been analyzed by means of Giemsa, C-banding, and silver-staining techniques. The present study substantially amends the first karyotype description and also analyzes in detail t...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-044
更新日期:1993-04-01 00:00:00
abstract::A putative old and ubiquitous interspersed DNA repeat family was identified from TaqI restriction, M13 cloning, and sequencing of the genomic DNA of a Mallard (Anas platyrhynchos), a Muscovy Duck (Cairina moschata), a Toulouse Goose (Anser anser), and a Black Swan (Cygnus atratus). A 425-bp consensus core sequence was...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g91-075
更新日期:1991-06-01 00:00:00
abstract::Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify ...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::We previously demonstrated that allopolyploidization could induce phenotypic variations and genome changes in a newly synthesized allotetraploid in Cucumis. To explore the molecular involvement of epigenetic phenomena, we investigated cytosine methylation in Cucumis by using methylation-sensitive amplified polymorphis...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-063
更新日期:2008-10-01 00:00:00
abstract::Light-harvesting-like (LIL) proteins are low-molecular-mass membrane proteins related to the light-harvesting complexes, which form the dominant antenna system in most photosynthetic eukaryotes. To analyze the LIL protein family, we mined a number of publicly available databases to identify members of this family in a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g09-081
更新日期:2010-01-01 00:00:00
abstract::To clarify the phylogenetic relationships of Carthamus species, we performed sequence analysis of the nuclear stearoyl acyl carrier protein desaturase (SACPD) gene and the chloroplast intergenic spacer region between leucine and phenylalanine tRNA genes (trnL-trnF IGS) in 13 taxa of Carthamus. The previous division of...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-059
更新日期:2008-09-01 00:00:00
abstract::The combination of two divergent genomes during hybridization can result in "genome shock". Although genome shock has been reported in the hybrids of some herbaceous plants, the pattern and the principle it follows are far from understood, especially in woody plants. Here, the gene expression patterns were remodeled i...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0105
更新日期:2018-07-01 00:00:00
abstract::Despite the recent advances in plant population genetic studies, the lack of information regarding pedigree, ploidy level, or mode of inheritance for many polyploids can compromise the analysis of the molecular data produced. The aim of this study was to examine both microsatellite and cytogenetic characteristics of t...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2013-0159
更新日期:2013-12-01 00:00:00
abstract::The first SSR-based genetic linkage map of Cucurbita moschata was created by integrating the maps of two F2 populations with one common parent developed from the crosses Waltham Butternut (WB) x Nigerian Local (NL) and ZHOU (a hull-less type) x WB. The integrated C. moschata map comprises 205 SSR markers and two morph...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-072
更新日期:2008-11-01 00:00:00
abstract::The complete sequence (15,455 bp) of the mitochondrial DNA of the mosquito Anopheles quadrimaculatus species A is reported. This genome is compact and very A+T rich (77.4% A+T). It contains genes for 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and 13 subunits of the mitochondrial inner membrane respiratory com...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-141
更新日期:1993-12-01 00:00:00
abstract::Southern blots of restriction fragments of genomic DNAs from Hordeum vulgare (L.), H. bulbosum (L.), and interspecific hybrids and their derivatives were hybridized with rDNA probe to identify locus-specific modifications at Rrn loci. H. bulbosum rDNA revealed a single EcoRV site per repeat compared with two sites in ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g99-053
更新日期:1999-12-01 00:00:00
abstract::The wild olive distribution extends from the Mediterranean region to south Asia and Austral Africa. The species is also invasive, particularly in Australia. Here, we investigated the sequence variation at five nuclear single-copy genes in 41 native and invasive accessions of the Mediterranean and African olive subspec...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2014-0001
更新日期:2014-03-01 00:00:00
abstract::The genus Cichorium consists of two widely cultivated species C. intybus (chicory) and C. endivia (endive) and four wild species, C. bottae, C. spinosum, C. calvum, and C. pumilum. A multivariate and an UPGMA (unweighted pair group method average) analysis based on AFLP (amplified fragment length polymorphism) markers...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-024
更新日期:2000-06-01 00:00:00
abstract::Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid (Aphis glycines Matsumura) has become the most damaging insect pest of...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g11-002
更新日期:2011-05-01 00:00:00
abstract::Electron microscopic examination of gonads of Drosophila melanogaster with different genotypes, including a metafemale 3X;2A and an intersex XXY;3A have revealed that the formation of synaptonemal complexes is controlled by the genic balance, i.e., the ratio of X chromosomes to autosomes. The Y chromosome is not invol...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g88-044
更新日期:1988-04-01 00:00:00
abstract::Southern hybridization analysis of Indian muntjac genomic DNA with the Eco-Taq bovine genomic protamine probe revealed a simple banding pattern. The pattern of hybridization was identical with that previously observed in the genus Bos. This suggested that the bovine probe specifically hybridized to the Indian muntjac ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g90-068
更新日期:1990-06-01 00:00:00
abstract::Wild Thinopyrum grasses are important gene pools for forage and cereal crops. Knowledge of their chromosome organizations is pivotal for efficient utilization of this important gene pool in germplasm enhancement programs. Expressed sequence tags derived simple sequence repeat (EST-SSR) markers for Thinopyrum bessarabi...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G10-088
更新日期:2010-12-01 00:00:00
abstract::Yams are species of the genus Dioscorea (family Dioscoreaceae), which consists of approximately 630 species. The majority of the world production of yams occurs in Africa with 58.8 million t annually, but they are also produced in the Americas and Asia. The saponins in yams have been reported to possess various proper...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0105
更新日期:2017-03-01 00:00:00
abstract::Cashew (Anacardium occidentale L.), introduced into India about 400 years ago, is distributed widely in the coastal regions as spontaneous populations as well as in cultivation. Despite the plant's commercial exploitation, little is known about its actual introduction and diversification. We attempted to construct the...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-119
更新日期:2009-03-01 00:00:00
abstract::Fire blight is a devastating bacterial disease of rosaceous plants. Its damage to apple production is a major concern, since no existing control option has proven to be completely effective. Some commercial apple varieties, such as 'Florina' and 'Nova Easygro', exhibit a consistent level of resistance to fire blight. ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-047
更新日期:2010-09-01 00:00:00
abstract::Genomic in situ hybridization (GISH) is one of the most popular and effective techniques for detecting alien chromatin introgressed into breeding lines; however, GISH analysis alone does not reveal the genetic identity of the alien chromosomes. We previously isolated a set of bacterial artificial chromosomes (BACs) sp...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Knowledge of the inheritance of C-glycosyl flavone synthesis in maize (Zea mays L.) silk tissues has been acquired through detailed genetic studies involving primarily germplasm from the Corn Belt Dent race. To test the robustness of this genetic knowledge, we examined C-glycosyl flavone synthesis in a genetically dis...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-100
更新日期:2009-01-01 00:00:00
abstract::Aegilops is a genus belonging to the family Poaceace, which have played an indispensible role in the evolution of bread wheat and continues to do so by transferring genes by wide hybridization. Being the secondary gene pool of wheat, gene transfer from Aegilops poses difficulties and segregation distortion is common. ...
journal_title:Genome
pub_type: 杂志文章,评审
doi:10.1139/gen-2017-0023
更新日期:2017-08-01 00:00:00