SNP discovery by amplicon sequencing and multiplex SNP genotyping in the allopolyploid species Brassica napus.

Abstract:

:Oilseed rape (Brassica napus) is an allotetraploid species consisting of two genomes, derived from B. rapa (A genome) and B. oleracea (C genome). The presence of these two genomes makes single nucleotide polymorphism (SNP) marker identification and SNP analysis more challenging than in diploid species, as for a given locus usually two versions of a DNA sequence (based on the two ancestral genomes) have to be analyzed simultaneously during SNP identification and analysis. One hundred amplicons derived from expressed sequence tag (ESTs) were analyzed to identify SNPs in a panel of oilseed rape varieties and within two sister species representing the ancestral genomes. A total of 604 SNPs were identified, averaging one SNP in every 42 bp. It was possible to clearly discriminate SNPs that are polymorphic between different plant varieties from SNPs differentiating the two ancestral genomes. To validate the identified SNPs for their use in genetic analysis, we have developed Illumina GoldenGate assays for some of the identified SNPs. Through the analysis of a number of oilseed rape varieties and mapping populations with GoldenGate assays, we were able to identify a number of different segregation patterns in allotetraploid oilseed rape. The majority of the identified SNP markers can be readily used for genetic mapping, showing that amplicon sequencing and Illumina GoldenGate assays can be used to reliably identify SNP markers in tetraploid oilseed rape and to convert them into successful SNP assays that can be used for genetic analysis.

journal_name

Genome

journal_title

Genome

authors

Durstewitz G,Polley A,Plieske J,Luerssen H,Graner EM,Wieseke R,Ganal MW

doi

10.1139/G10-079

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

948-56

issue

11

eissn

0831-2796

issn

1480-3321

pii

g10-079

journal_volume

53

pub_type

杂志文章

相关文献

GENOME文献大全
  • Likelihood estimation of quantitative genetic parameters when selection occurs: models and problems.

    abstract::Conceptual aspects of estimation of genetic components of variance and covariance under selection are discussed, with special attention to likelihood methods. Certain selection processes are described and alternative likelihoods that can be used for analysis are specified. There is a mathematical relationship between ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g89-136

    authors: Gianola D,Fernando RL,Im S,Foulley JL

    更新日期:1989-01-01 00:00:00

  • Microsatellite marker analysis of an anther-derived potato family: skewed segregation and gene-centromere mapping.

    abstract::Segregation patterns of polymorphic simple sequence repeat (SSR) primer pairs were investigated in monoploid potato families derived from anther culture. A total of 14 primers developed from the sequences in the database, as well as from a genomic library of potato, was used. Distorted segregation was observed for sev...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g01-140

    authors: Chani E,Ashkenazi V,Hillel J,Veilleux RE

    更新日期:2002-04-01 00:00:00

  • SSR-based genetic linkage map of Cucurbita moschata and its synteny with Cucurbita pepo.

    abstract::The first SSR-based genetic linkage map of Cucurbita moschata was created by integrating the maps of two F2 populations with one common parent developed from the crosses Waltham Butternut (WB) x Nigerian Local (NL) and ZHOU (a hull-less type) x WB. The integrated C. moschata map comprises 205 SSR markers and two morph...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G08-072

    authors: Gong L,Pachner M,Kalai K,Lelley T

    更新日期:2008-11-01 00:00:00

  • Simple sequence repeats in Cucumis mapping and map merging.

    abstract::Thirty-four polymorphic simple-sequence repeats (SSRs) were evaluated for length polymorphism in melon (Cucumis melo L.) and cucumber (Cucumis sativus L.). SSR markers were located on three melon maps (18 on the map of 'Vedrantais' and PI 161375, 23 on the map of 'Piel de Sapo' and PI 161375, and 16 on the map of PI 4...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Danin-Poleg Y,Reis N,Baudracco-Arnas S,Pitrat M,Staub JE,Oliver M,Arus P,deVicente CM,Katzir N

    更新日期:2000-12-01 00:00:00

  • DNA barcodes and citizen science provoke a diversity reappraisal for the "ring" butterflies of Peninsular Malaysia (Ypthima: Satyrinae: Nymphalidae: Lepidoptera).

    abstract::The "rings" belonging to the genus Ypthima are amongst the most common butterflies in Peninsular Malaysia. However, the species can be difficult to tell apart, with keys relying on minor and often non-discrete ring characters found on the hindwing. Seven species have been reported from Peninsular Malaysia, but this is...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0156

    authors: Jisming-See SW,Sing KW,Wilson JJ

    更新日期:2016-10-01 00:00:00

  • Identification and analysis of homoeologous segments of the genomes of rice and Arabidopsis thaliana.

    abstract::Using contiguous genomic DNA sequences of Arabidopsis thaliana, we were able to identify a region of conserved structure in the genome of rice. The conserved, and presumptive homoeologous segments, are 194 kb and 219-300 kb in size in Arabidopsis and rice, respectively. They contain five homologous genes, distinguishe...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: van Dodeweerd AM,Hall CR,Bent EG,Johnson SJ,Bevan MW,Bancroft I

    更新日期:1999-10-01 00:00:00

  • Variation among the dispersed (GATA)n sequences in Drosophila melanogaster.

    abstract::Five nonallelic copies of the dispersed (GATA)n repeated sequence of Drosophila melanogaster (referred to as GATA elements) have been sequenced and analysed. The GATA elements range in size from 111 to 444 bp, consisting predominantly of tandemly repeated GATAs, interspersed with variants of the subunit. The types and...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g90-113

    authors: Simpson PR

    更新日期:1990-10-01 00:00:00

  • New karyotypes of Atlantic tree rats, genus Phyllomys (Rodentia: Echimyidae).

    abstract::Phyllomys (Echimyidae, Rodentia) is a genus of Neotropical rodents with available cytogenetic data restricted to six out of 13 species, mainly based on simple staining methods, without detailed analyses. In this work, we present new karyotypes for Phyllomys lamarum (diploid number 2n = 56, fundamental number or number...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2013-0168

    authors: Araújo NP,Loss AC,Cordeiro-Junior DA,da Silva KR,Leite YL,Svartman M

    更新日期:2014-01-01 00:00:00

  • Relationship between pachytene synapsis, metaphase I associations, and transmission of 2B and 4B chromosomes in rye.

    abstract::2B rye plants selected for high (H) or low (L) B transmission rate were studied at pachytene and metaphase I of meiosis to determine the relationship between synapsis, bivalents at metaphase I, and B transmission rate. The results show that the 2 B chromosomes (Bs) form bivalents at pachytene in both the H and L lines...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Jiménez G,Manzanero S,Puertas MJ

    更新日期:2000-04-01 00:00:00

  • Isolation of a new species-specific repetitive sequence from Thinopyrum elongatum and its use in the studies of alien translocations.

    abstract::A new repetitive sequence that is extremely abundant and well dispersed in the Thinopyrum elongatum genome but present in low-copy number in wheat (Triticum aestivum L.) has been isolated. This repeat and a Th. elongatum repeat isolated in another laboratory were used to identify cosmid genomic clones containing the r...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-012

    authors: Bournival B,Obanni M,Abad A,Ohm H,Mackenzie S

    更新日期:1994-02-01 00:00:00

  • Molecular cloning, heterologous expression, and phylogenetic analysis of a novel y-type HMW glutenin subunit gene from the G genome of Triticum timopheevi.

    abstract::A novel y-type high molecular weight (HMW) glutenin subunit gene from the G genome of Triticum timopheevi (2n=4x=28, AAGG) was isolated and characterized. Genomic DNA from accession CWI17006 was amplified and a 2200 bp fragment was obtained. Sequence analysis revealed a complete open reading frame including N- and C-t...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G07-089

    authors: Li X,Zhang Y,Gao L,Wang A,Ji K,He Z,Appels R,Ma W,Yan Y

    更新日期:2007-12-01 00:00:00

  • Robustness of QTLs across germplasm pools using a model quantitative trait.

    abstract::Knowledge of the inheritance of C-glycosyl flavone synthesis in maize (Zea mays L.) silk tissues has been acquired through detailed genetic studies involving primarily germplasm from the Corn Belt Dent race. To test the robustness of this genetic knowledge, we examined C-glycosyl flavone synthesis in a genetically dis...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G08-100

    authors: Lee EA,Staebler JM,Grainger C,Snook ME

    更新日期:2009-01-01 00:00:00

  • De novo balanced complex chromosome rearrangements involving chromosomes 1B and 3B of wheat and 1R of rye.

    abstract::Complex chromosome rearrangements (CCRs) are defined as structural abnormalities involving more than two chromosome breaks, coupled with exchanges of chromosomal segments. Information on CCRs in plants is limited. In the present study, a plant (26-4) harboring translocation chromosomes 1RS.1BL and 4RS.4DL was selected...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0112

    authors: Ren T,Li Z,Yan B,Tan F,Tang Z,Fu S,Yang M,Ren Z

    更新日期:2016-12-01 00:00:00

  • Mitotic and polytene chromosome analysis in Dacus oleae (Diptera: Tephritidae).

    abstract::The present study constitutes the first attempt to construct a photographic map of the polytene chromosomes of Dacus oleae, a pest of the olive tree that causes serious financial damage in all olive oil producing countries. The map was constructed by using the larval fat body cells, the chromosomes of which are repres...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g92-056

    authors: Mavragani-Tsipidou P,Karamanlidou G,Zacharopoulou A,Koliais S,Kastritisis C

    更新日期:1992-06-01 00:00:00

  • Genetic analysis and mapping of a thermosensitive genic male sterility gene, tms6(t), in rice (Oryza sativa L.).

    abstract::Thermosensitive genic male-sterile (TGMS) rice is widely used in hybrid rice production. Because of its specific temperature requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered TGMS line, G20S, has an opposite phenotype compared with normal TGMS lines. G20S is completely sterile ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g09-092

    authors: Liu X,Li X,Zhang X,Wang S

    更新日期:2010-02-01 00:00:00

  • Chromosome elimination in germ line-soma differentiation of Acricotopus lucidus (Diptera, Chironomidae).

    abstract::During germ line-soma differentiation in early syncytial embryonic development of the chironomid Acricotopus lucidus, a complement of supernumerary chromosomes, the so-called germ line limited chromosomes (Ks), is excluded from the future somatic nuclei in the course of elimination mitoses. The Ks lag behind in the eq...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-103

    authors: Staiber W

    更新日期:2006-03-01 00:00:00

  • Development of a set of near isogenic and backcross recombinant inbred lines containing most of the Lycopersicon hirsutum genome in a L. esculentum genetic background: a tool for gene mapping and gene discovery.

    abstract::A novel population consisted of a set of 99 near isogenic lines (NILs) and backcross recombinant inbred lines (BCRILs) derived from a cross between the cultivated tomato Lycopersicon esculentum cv. E6206 and L. hirsutum accession LA1777 is presented. Most of the lines contain a single defined introgression from L. hir...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Monforte AJ,Tanksley SD

    更新日期:2000-10-01 00:00:00

  • A family of centromeric satellite DNAs from the European brown frog Rana graeca italica.

    abstract::Digestion of Rana graeca italica DNA with Asp 718I produces highly repetitive fragments of 281 and 385 bp that were cloned and sequenced. The shorter fragment corresponds to the unit repeat (RgiS1b) of a satellite DNA. The longer fragment was found to be part of a 494-bp repeat of another satellite DNA (RgiS1a) that w...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g97-800

    authors: Cardone DE,Feliciello I,Marotta M,Rosati C,Chinali G

    更新日期:1997-10-01 00:00:00

  • DNA content of heterochromatin and euchromatin in tomato (Lycopersicon esculentum) pachytene chromosomes.

    abstract::Lycopersicon esculentum (tomato) has a small genome (2C = 1.90 pg of DNA) packaged in 2n = 2x = 24 small acrocentric to metacentric chromosomes. Like the chromosomes of other members of the family Solanaceae, tomato chromosomes have pericentromeric heterochromatin. To determine the fraction of the tomato genome found ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-011

    authors: Peterson DG,Stack SM,Price HJ,Johnston JS

    更新日期:1996-02-01 00:00:00

  • Transcriptome analysis reveals hybridization-induced genome shock in an interspecific F1 hybrid from Camellia.

    abstract::The combination of two divergent genomes during hybridization can result in "genome shock". Although genome shock has been reported in the hybrids of some herbaceous plants, the pattern and the principle it follows are far from understood, especially in woody plants. Here, the gene expression patterns were remodeled i...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2017-0105

    authors: Zhang M,Liu XK,Fan W,Yan DF,Zhong NS,Gao JY,Zhang WJ

    更新日期:2018-07-01 00:00:00

  • A search for diagnostic AFLP markers in Cichorium species with emphasis on endive and chicory cultivar groups.

    abstract::The genus Cichorium consists of two widely cultivated species C. intybus (chicory) and C. endivia (endive) and four wild species, C. bottae, C. spinosum, C. calvum, and C. pumilum. A multivariate and an UPGMA (unweighted pair group method average) analysis based on AFLP (amplified fragment length polymorphism) markers...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g00-024

    authors: Kiers AM,Mes TH,van der Meijden R,Bachmann K

    更新日期:2000-06-01 00:00:00

  • Analysis of QTLs for yield components, agronomic traits, and disease resistance in an advanced backcross population of spring barley.

    abstract::Hordeum vulgare subsp. spontaneum, the wild progenitor of barley, is a potential source of useful genetic variation for barley breeding programs. The objective of this study was to map quantitative trait loci (QTLs) in an advanced backcross population of barley. A total of 207 BC3 lines were developed using the 2-rowe...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-128

    authors: Li JZ,Huang XQ,Heinrichs F,Ganal MW,Röder MS

    更新日期:2006-05-01 00:00:00

  • Genetic analysis and historical perspective of cashew (Anacardium occidentale L.) introduction into India.

    abstract::Cashew (Anacardium occidentale L.), introduced into India about 400 years ago, is distributed widely in the coastal regions as spontaneous populations as well as in cultivation. Despite the plant's commercial exploitation, little is known about its actual introduction and diversification. We attempted to construct the...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G08-119

    authors: Archak S,Gaikwad AB,Swamy KR,Karihaloo JL

    更新日期:2009-03-01 00:00:00

  • Cytological and molecular analysis of centromere misdivision in maize.

    abstract::B chromosome derivatives suffering from breaks within their centromere were examined cytologically and molecularly. We showed by high resolution FISH that misdivision of the centromere of a univalent chromosome can occur during meiosis. The breaks divide the centromere repeat sequence cluster. A telocentric chromosome...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-023

    authors: Kaszás E,Kato A,Birchler JA

    更新日期:2002-08-01 00:00:00

  • Three new satellite sequences and a mobile element found inside HSP70 introns of the Mediterranean mussel (Mytilus galloprovincialis).

    abstract::We report the characterization of 3 new repetitive sequences from the bivalve mollusc Mytilus galloprovincialis, designated Mg1, Mg2, and Mg3, with monomer lengths of 169, 260, and 70 bp, respectively. The 3 repeats together constitute approximately 7.8% of the M. galloprovincialis genome and were found, together with...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-111

    authors: Kourtidis A,Drosopoulou E,Pantzartzi CN,Chintiroglou CC,Scouras ZG

    更新日期:2006-11-01 00:00:00

  • Development of soybean aphid genomic SSR markers using next generation sequencing.

    abstract::Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid (Aphis glycines Matsumura) has become the most damaging insect pest of...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g11-002

    authors: Jun TH,Michel AP,Mian MA

    更新日期:2011-05-01 00:00:00

  • Variations in the heat-induced protein pattern of several Drosophila montium subgroup species (Diptera:Drosophilidae).

    abstract::After temperature elevation, the newly synthesized polypeptides from several Drosophila montium subgroup species, of the melanogaster species group, were analyzed in denaturing acrylamide gels. The pattern obtained is characteristic of the heat shock response already documented for many other Drosophila species, altho...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g97-019

    authors: Konstantopoulou I,Drosopoulou E,Scouras ZG

    更新日期:1997-02-01 00:00:00

  • Insect mitochondrial genomics 2: The complete mitochondrial genome sequence of a giant stonefly, Pteronarcys princeps, asymmetric directional mutation bias, and conserved plecopteran A+T-region elements.

    abstract::Mitochondrial (mt) genome sequences of insects are receiving renewed attention in molecular phylogentic studies, studies of mt-genome rearrangement, and other unusual molecular phenomena, such as translational frameshifting. At present, the basal neopteran lineages are poorly represented by mt-genome sequences. Comple...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-037

    authors: Stewart JB,Beckenbach AT

    更新日期:2006-07-01 00:00:00

  • Tomato chromosome 6: effect of alien chromosomal segments on recombinant frequencies.

    abstract::Variation in recombinant frequencies at two adjacent intervals on chromosome 6 of tomato (Lycopersicon esculentum Mill.) has been studied in seven lines that differ in the amount and origin of introgressed segments from wild species. These lines were all crossed to a genotype homozygous recessive for the markers tl, y...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-062

    authors: Liharska T,Wordragen M,Kammen A,Zabel P,Koornneef M

    更新日期:1996-06-01 00:00:00

  • Linkage and mapping analyses of the densonucleosis non-susceptible gene nsd-Z in the silkworm Bombyx mori using SSR markers.

    abstract::In the silkworm Bombyx mori, non-susceptibility to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is located on chromosome 15. Owing to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progeny were used for...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-125

    authors: Li M,Guo Q,Hou C,Miao X,Xu A,Guo X,Huang Y

    更新日期:2006-04-01 00:00:00