Construction of a garlic BAC library and chromosomal assignment of BAC clones using the FISH technique.

abstract：:The molecular analyses of three of the regulatory genes (transformer (tra), doublesex (dsx), and transformer-2 (tra-2)) controlling sexual differentiation in Drosophila have demonstrated that the control of RNA processing has a major role in regulating somatic sexual differentiation. The activities of both the tra and...

journal_title：Genome

authors： Baker BS,Burtis K,Goralski T,Mattox W,Nagoshi R

更新日期：1989-01-01 00:00:00

abstract：:We investigated the 5S ribosomal RNA (rRNA) genes of the isopod crustacean Asellus aquaticus. Using PCR amplification, three different tandemly repeated units containing 5S rDNA were identified. Two of the three sequences were cloned and sequenced. One of them was 1842 bp and presented a 5S rRNA gene and a U1 small nu...

journal_title：Genome

authors： Pelliccia F,Barzotti R,Bucciarelli E,Rocchi A

更新日期：2001-06-01 00:00:00

abstract：:Nematodes are frequently cited as underrepresented in faunistic surveys using DNA barcoding with COI. This underrepresentation is generally attributed to a limited presence of nematodes in DNA databases which, in turn, is often ascribed to structural variability and high evolutionary rates in nematode mitochondrial ge...

journal_title：Genome

authors： Powers TO,Harris TS,Higgins RS,Mullin PG,Powers KS

更新日期：2020-06-11 00:00:00

abstract：:To investigate the effect of signal molecules and their receptor-associated genes on rat liver regeneration (LR) at the transcriptional level, the associated genes were originally obtained by retrieving the databases and related scientific publications; their expression profiles in rat LR were then checked using the R...

journal_title：Genome

authors： Xu CS,Shao HY,Du B

更新日期：2009-06-01 00:00:00

abstract：:The present work compares the molecular affinities, revealed by GISH, with the analysis of meiotic pairing in intra- and interspecific hybrids between species of Zea obtained in previous works. The joint analysis of these data provided evidence about the evolutionary relationships among the species from the paleopolyp...

journal_title：Genome

authors： González GE,Poggio L

更新日期：2015-10-01 00:00:00

abstract：:Abstract: Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males highlighted the W chromosomes in mitotic plates and the W chromatin in polyploid interphase nuclei of the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria ...

journal_title：Genome

authors： Sahara K,Marec F,Eickhoff U,Traut W

更新日期：2003-04-01 00:00:00

abstract：:Genetic variation in Gir cattle (Bos indicus) has so far not been well characterized. In this study, we used whole genome sequencing of three Gir bulls and a pooled sample from another 11 bulls to identify polymorphisms and loci under selection. A total of 9 990 733 single nucleotide polymorphisms (SNPs) and 604 308 i...

journal_title：Genome

authors： Liao X,Peng F,Forni S,McLaren D,Plastow G,Stothard P

更新日期：2013-10-01 00:00:00

abstract：:Plant disease resistance gene analog (RGA) markers were designed according to the conserved sequence of known RGAs and used to map resistance genes. We used genome-wide RGA markers for genetic analyses of structure and diversity in a global rice germplasm collection. Of the 472 RGA markers, 138 were polymorphic and th...

journal_title：Genome

authors： Ren J,Yu Y,Gao F,Zeng L,Lu X,Wu X,Yan W,Ren G

更新日期：2013-07-01 00:00:00

abstract：:Spermatocytes at meiotic metaphase I and anaphase I have a characteristic centromeric filament in a variety of vertebrate organisms. This centromeric filament was first demonstrated on mouse spermatocytes and its presence is now extended to spermatocytes from the human, rat, golden hamster, bull, and chicken. The visu...

journal_title：Genome

authors： Solari AJ,Tandler CJ

更新日期：1991-12-01 00:00:00

abstract：:Carrot (Daucus carota L.) chromosomes are small and poorly differentiated in size and morphology. Here we demonstrate that fluorescent in situ hybridization (FISH) signals derived from arbitrary PCR probes can be used for chromosome identification in carrot. To prepare probes, we searched for nonpolymorphic products a...

journal_title：Genome

authors： Nowicka A,Grzebelus E,Grzebelus D

更新日期：2012-03-01 00:00:00

abstract：:Canada wild rye (CWR, Elymus canadensis L., 2n = 4x = 28) is a potential source of genes for disease resistance and environmental tolerance in barley (Hordeum vulgare L., 2n = 2x = 14). Tissue cultures were initiated from immature inflorescences of CWR x 'Betzes' barley hybrids to promote CWR introgression into barley...

journal_title：Genome

authors： Dahleen LS

更新日期：1996-04-01 00:00:00

abstract：:Microsatellites or simple sequence repeats (SSRs) were isolated from coconut (Cocos nucifera) and tested for polymorphism on restricted germplasm. Sequencing of 197 clones from a cv. Tagnanan Tall-enriched genomic library showed that 75% contained a microsatellite, of which 64% were dinucleotide (GA/CT, CA/GT and GC/C...

journal_title：Genome

authors： Rivera R,Edwards KJ,Barker JH,Arnold GM,Ayad G,Hodgkin T,Karp A

更新日期：1999-08-01 00:00:00

abstract：:Aegilops is a genus belonging to the family Poaceace, which have played an indispensible role in the evolution of bread wheat and continues to do so by transferring genes by wide hybridization. Being the secondary gene pool of wheat, gene transfer from Aegilops poses difficulties and segregation distortion is common. ...

journal_title：Genome

authors： Niranjana M

更新日期：2017-08-01 00:00:00

abstract：:Polyploidization is one of the major driving forces in plant evolution and is extremely relevant to speciation and diversity creation. Polyploidization leads to a myriad of genetic and epigenetic alterations that ultimately generate plants and species with increased genome plasticity. Polyploids are the result of the ...

journal_title：Genome

authors： Bento M,Gustafson JP,Viegas W,Silva M

更新日期：2011-03-01 00:00:00

abstract：:The use of complementary RNA sequences such as antisense RNAs and ribozymes to regulate the expression of specific genes in eukaryotic cells has been well-documented, particularly with their application to both human gene therapy and plant biotechnology. Despite the simplicity of this approach, this technique usually ...

journal_title：Genome

authors： Arndt GM,Rank GH

更新日期：1997-12-01 00:00:00

abstract：:Cytogenetic studies in Triticum monococcum (2n = 2x = 14, AA) were initiated by generating a series of primary as well as double and triple trisomics from autotriploids derived from crosses between induced autotetraploids and a diploid progenitor. Analysis of meiotic chromosome behaviour revealed that, with the except...

journal_title：Genome

authors： Kim NS,Kuspira J

更新日期：1993-06-01 00:00:00

abstract：:Three long terminal repeat (LTR) sequences of Ty1-copia retrotransposons were identified in cucumber (Cucumis sativus L.) and named Tcs 1, Tcs 2, and Tcs 3. A sequence-specific amplification polymorphism (SSAP) marker system based on these LTR sequences displayed a higher level of polymorphism than AFLPs in cucumber. ...

journal_title：Genome

authors： Lou Q,Chen J

更新日期：2007-09-01 00:00:00

abstract：:The timing and rate of genomic variation induced by allopolyploidization in the intergeneric wheat-rye (Triticum spp. - Secale cereale L.) hybrid triticale (x Triticosecale Wittmack) was studied using amplified fragment length polymorphism (AFLP) analyses with 2 sets of primers, EcoRI-MseI (E-M) and PstI-MseI (P-M), w...

journal_title：Genome

authors： Ma XF,Gustafson JP

更新日期：2006-08-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads...

journal_title：Genome

authors： Gómez MI,Islam-Faridi MN,Woo SS,Czeschin D Jr,Zwick MS,Stelly DM,Price HJ,Schertz KF,Wing RA

更新日期：1997-08-01 00:00:00

abstract：:Multiple-target fluorescence in situ hybridization (FISH) was applied on mitotic chromosomes of seven Lens taxa using two highly repetitive sequences (pLc30 and pLc7) isolated from the cultivated lentil and the multigene families for the 18S-5.8S-25S (pTa71) and 5S rRNA (pTa794) from wheat simultaneously as probes. Th...

journal_title：Genome

authors： Galasso I

更新日期：2003-12-01 00:00:00

abstract：:A putative old and ubiquitous interspersed DNA repeat family was identified from TaqI restriction, M13 cloning, and sequencing of the genomic DNA of a Mallard (Anas platyrhynchos), a Muscovy Duck (Cairina moschata), a Toulouse Goose (Anser anser), and a Black Swan (Cygnus atratus). A 425-bp consensus core sequence was...

journal_title：Genome

authors： Zhu X,Burgoyne LA,Skinner JD

更新日期：1991-06-01 00:00:00

abstract：:We examined the sequence of a 2214 base pair HindIII fragment from the mitochondrial genome of six rainbow trout. The fragment encodes four proteins and two tRNAs. Sequences for two fish from a single locality were identical. Those from separate localities differed by from 1 to 7 nucleotide substitutions. Of 13 variab...

journal_title：Genome

authors： Beckenbach AT,Thomas WK,Sohrabi H

更新日期：1990-02-01 00:00:00

abstract：:Using genomic in situ hybridization to chromosomes, we identified introgressed segments in a tetrageneric hybrid of Triticum, Avena, Thinopyrum, and Secale, which conferred high resistance to leaf rust, stem rust, stripe rust, powdery mildew, and root rot to wheat. The disease-resistance traits of the hybrid originate...

journal_title：Genome

authors： Tang S,Zhuang J,Wen Y,Ai SA,Li H,Xu J

更新日期：1997-02-01 00:00:00

abstract：:Selection of wheat germplasm for a range of quality traits has been a challenging exercise because of the cost of testing, the variation within testing data, and a poor understanding of the underlying genetics. The objective of this study was to identify quantitative trait loci (QTLs) underlying quality traits in whea...

journal_title：Genome

authors： Raman R,Allen H,Diffey S,Raman H,Martin P,McKelvie K

更新日期：2009-08-01 00:00:00

abstract：:The DOPA decarboxylase gene (Ddc) belongs to the "early-late" class of ecdysone-inducible genes in Drosophila melanogaster. Its expression is up-regulated in epidermal tissues by the ecdysone receptor acting through a response element, EcRE. In this paper, we show that another member of the nuclear receptor superfamil...

journal_title：Genome

authors： Davis MM,Yang P,Chen L,O'Keefe SL,Hodgetts RB

更新日期：2007-11-01 00:00:00

abstract：:DNA gel-blot and in situ hybridization with genome-specific repeated sequences have proven to be valuable tools in analyzing genome structure and relationships in species with complex allopolyploid genomes such as hexaploid oat (Avena sativa L., 2n = 6x = 42; AACCDD genome). In this report, we describe a systematic ap...

journal_title：Genome

authors： Ananiev EV,Vales MI,Phillips RL,Rines HW

更新日期：2002-04-01 00:00:00

abstract：:Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. In the present study, 3 cDNA libraries generated by the Bean EST project (http://lgm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genom...

journal_title：Genome

authors： Hanai LR,de Campos T,Camargo LE,Benchimol LL,de Souza AP,Melotto M,Carbonell SA,Chioratto AF,Consoli L,Formighieri EF,Siqueira MV,Tsai SM,Vieira ML

更新日期：2007-03-01 00:00:00

abstract：:In an initial investigation of differential expression of genes caused by cis-acting regulatory elements in rice, the lack of reproducibility led us to question the basic premise of allelic expression imbalance determination: namely that departures of cDNA expression ratios from those observed in genomic DNA provide u...

journal_title：Genome

authors： Akkinepalli H,Ereful N,Liu Y,Malabanan K,Howells R,Stamati K,Powell W,Leung H,Greenland A,Mackay I,Lee D

更新日期：2012-05-01 00:00:00

abstract：:Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as g...

journal_title：Genome

authors： Echt CS,Erdahl LA,McCoy TJ

更新日期：1992-02-01 00:00:00

abstract：:We have characterized a so-called D genome specific repetitive DNA sequence (pAs1) of Aegilops squarrosa L. (2n = 14, genome DD) with respect to its DNA sequence and its distribution among Triticeae species. The clone consisted of three units of a repetitive DNA sequence of 336 or 337 base pairs, and was AT rich (65.2...

journal_title：Genome

authors： Nagaki K,Tsujimoto H,Isono K,Sasakuma T

更新日期：1995-06-01 00:00:00