Abstract：
:Neutrophils are key effector cells of the innate immune system and are involved in the host defense against invading pathogens such as viruses. Recently, it was reported that HIV-1-neutrophil interaction triggers neutrophil activation and promotes expression of Toll-like receptors (TLRs). Here, we assessed the role of single-stranded RNA40 (ssRNA40) derived from HIV-1 in neutrophil activation. We observed functional activation of neutrophils in response to HIV-1-derived ssRNA40 based on the expression of TLR7/8, RIG-I, and MDA5, induction of cytokines (IL-6 and TNF-α), and the production of reactive oxygen species (ROS). Additionally, ssRNA40 promoted the expression of CD62L and TNF-α and the production of ROS in the presence of the TLR2 agonist Pam2CSK4. ssRNA40 together with R848 (a TLR7/8 agonist) increased CD11b expression but decreased CD62L expression. Furthermore, decreased IL-6 expression was observed in the presence of the TLR4 agonist LPS. Finally, we found that ssRNA40 promotes RIG-I and MDA5 expression in the presence of the TLR2, TLR4 and TLR7/8 agonists. This study demonstrates a functional response of TLRs in neutrophils challenged with ssRNA40, suggesting that TLRs could be involved in the innate immune response observed during HIV infection, which might be mediated by its genome.
journal_name
Immunol Resjournal_title
Immunologic researchauthors
Giraldo DM,Hernandez JC,Urcuqui-Inchima Sdoi
10.1007/s12026-016-8876-9subject
Has Abstractpub_date
2016-12-01 00:00:00pages
1185-1194issue
5-6eissn
0257-277Xissn
1559-0755pii
10.1007/s12026-016-8876-9journal_volume
64pub_type
杂志文章abstract：:Activation-induced cell death (AICD) has been demonstrated in T-cell hybridomas, immature thymocytes, and activated mature T cells. However, the molecular mechanisms of AICD and its physiological role in T-helper-cell differentiation remain uncertain. Recently, we have shown that Th1 and Th2 cells have distinct mechan...
journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2003-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 历史文章,杂志文章
doi：10.1385/IR:36:1:1
更新日期：2006-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
doi：10.1007/s12026-017-8931-1
更新日期：2017-08-01 00:00:00
abstract：:Mast cells (MC) are important effector cells in allergic disorders. Recently, the role of MC in innate and adaptive immunity is gaining prominence. Nitric oxide is an important signaling molecule and its production in mast cell has been reported widely. However, controversy exists about whether MC produce NO. This rev...
journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2005-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1007/s12026-014-8562-8
更新日期：2015-03-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2019-02-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1007/s12026-017-8976-1
更新日期：2018-02-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
doi：10.1007/s12026-013-8448-1
更新日期：2013-12-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2006-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2003-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2000-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2005-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
doi：10.1007/BF02918171
更新日期：1995-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
doi：10.1007/s12026-007-0070-7
更新日期：2007-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
doi：10.1007/s12026-009-8130-9
更新日期：2010-03-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
doi：10.1385/IR:26:1-3:323
更新日期：2002-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2000-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1007/s12026-013-8482-z
更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2015-02-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2012-04-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1007/s12026-013-8456-1
更新日期：2013-12-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,评审
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更新日期：2004-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1007/BF02917584
更新日期：1986-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s12026-017-8917-z
更新日期：2017-08-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1007/s12026-015-8778-2
更新日期：2016-06-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1385/IR:23:2-3:253
更新日期：2001-01-01 00:00:00
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journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1007/s12026-016-8877-8
更新日期：2017-06-01 00:00:00
abstract：:Invariant CD1d-restricted natural killer T (iNKT) cells function during innate and adaptive immune responses. A functional and numerical deficiency of iNKT cells is well documented in both nonobese diabetic (NOD) mice and humans with autoimmune type 1 diabetes (T1D). Restoring the numerical and/or functional deficienc...
journal_title：Immunologic research
pub_type： 杂志文章,评审
doi：10.1385/IR:31:3:177
更新日期：2005-01-01 00:00:00
abstract：:Primate complement receptor type 1 (CR1) protein, a single-chain transmembrane glycoprotein, plays an important role in immune adherence and clearing complement-opsonized immune complexes. Here, the mRNA of the porcine primate-like complement receptor (CR1-like) gene was analyzed, and two domain sequences with potenti...
journal_title：Immunologic research
pub_type： 杂志文章
doi：10.1007/s12026-016-8792-z
更新日期：2016-08-01 00:00:00
