Abstract:
:Dendritic cells (DCs) are known to play an important role in initiating and orchestrating antimicrobial immunity. Given the fact that candidiasis appears often in immunocompromised patients, it seems plausible that DCs hold the key to new antifungal strategies. One possibility to enhance the potency of DC-based immunotherapy is to silence the negative immunoregulatory pathways through the ablation suppressor of cytokine signaling suppressor 1 (SOCS1). Here, we deliver small interfering RNA (siRNA) against SOCS1 into murine bone marrow DCs, and as a consequence, we investigate the maturation/action of DCs and the subsequent T cell response after exposure to C. albicans. Our results show that the maturation of DCs (i.e., expressions of CD80, CD40, CD86, and MHC II) are significantly increased in the silenced SOCS1 treatment group after exposure to C. albicans. As a result, suppression of the SOCS1 promotes the greatest expression of IFN-γ and IL-12, and reduces IL-4 secretions, which induce CD4(+) cell Th1 differentiation but inactivate Th2 cell development. The responses of IL-6 and TNF-β consist of up-regulation in the presence of C. albicans, but this is not specific to SOCS1 silencing, suggesting that these cytokines are not regulated by the SOCS1 gene in fungal infections. We find Th17 differentiation is unchanged regardless of SOCS1 inhibition. The increase in phagocytosis and killing of C. albicans in SOCS1 gene-treated DCs indicate a role for this cytokine suppressor in innate immunity as well. In conclusion, our findings support the view that SOCS1 protein is a critical inhibitory molecule for controlling cytokine response and antigen presentation by DCs, thereby regulating the magnitude of innate and adaptive immunities by generating IFN-γ-production T cells (Th1)--but not Th17--from naïve CD4(+) T cells. Our study demonstrates that SOCS1 siRNA can serve as a useful vehicle to modulate the function of DCs against C. albicans infection.
journal_name
Immunol Resjournal_title
Immunologic researchauthors
Shi D,Li D,Yin Q,Qiu Y,Yan H,Shen Y,Lu G,Liu Wdoi
10.1007/s12026-014-8562-8subject
Has Abstractpub_date
2015-03-01 00:00:00pages
206-18issue
3eissn
0257-277Xissn
1559-0755journal_volume
61pub_type
杂志文章abstract::Antimicrobial peptide innate immunity plays a central role in the susceptibility to infectious diseases, as has been described extensively in different settings. However, the role that these molecules play in the immunity mediated by polymorphonuclear phagocytes as part of the innate immunity of ageing individuals has...
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journal_title:Immunologic research
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journal_title:Immunologic research
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journal_title:Immunologic research
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journal_title:Immunologic research
pub_type: 杂志文章
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journal_title:Immunologic research
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doi:10.1007/s12026-013-8456-1
更新日期:2013-12-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章,评审
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更新日期:2002-01-01 00:00:00
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更新日期:2016-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s12026-019-09107-6
更新日期:2019-10-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章,评审
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更新日期:2015-06-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章,评审
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更新日期:2005-01-01 00:00:00
abstract::Histones appear in plasma during infectious or non-infectious sepsis and are associated with multiorgan injury. In the current studies, intravenous infusion of histones resulted in their localization in major organs. In vitro exposure of mouse macrophages to histones caused a buildup of histones on cell membranes foll...
journal_title:Immunologic research
pub_type: 杂志文章
doi:10.1007/s12026-015-8628-2
更新日期:2015-03-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章,评审
doi:10.1007/s12026-012-8345-z
更新日期:2013-03-01 00:00:00
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更新日期:2015-02-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章,评审
doi:10.1007/s12026-009-8102-0
更新日期:2009-12-01 00:00:00
abstract::Vaccination represents one of the most efficacious and cost-effective medical interventions. It is the only medical intervention proven to eliminate disease at a global level. Many of the pathogens against which we most require adequate vaccines infect via the highly exposed mucosal surfaces. For this reason the mucos...
journal_title:Immunologic research
pub_type: 杂志文章,评审
doi:10.1385/IR:30:1:035
更新日期:2004-01-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章
doi:10.1007/s12026-011-8215-0
更新日期:2011-08-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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doi:10.1007/s12026-020-09136-6
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pub_type: 历史文章,杂志文章,评审
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更新日期:2005-01-01 00:00:00
abstract::A 20-year-old man developed severe abdominal pain 1 week after being vaccinated with the first dose of quadrivalent human papillomavirus (HPV) vaccine (Gardasil®). Despite ongoing symptoms of nausea and pain, he received the second dose of the vaccine. Only 10 days later, laboratory results revealed significantly elev...
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更新日期:2017-02-01 00:00:00
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更新日期:2018-02-01 00:00:00
abstract::Allogeneic hematopoietic stem cell transplantation (HSCT) currently provides the only chance of curative therapy for many patients with hematological malignancies. Owing to the excess morbidity and mortality observed in less robust patients, this approach has traditionally been limited to younger patients without sign...
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更新日期:2003-01-01 00:00:00
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更新日期:2015-02-01 00:00:00
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更新日期:1992-01-01 00:00:00
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更新日期:2008-01-01 00:00:00
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更新日期:2008-01-01 00:00:00
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更新日期:2019-02-01 00:00:00