Complement receptor activity of recombinant porcine CR1-like protein expressed in a eukaryotic system.

Abstract:

:Primate complement receptor type 1 (CR1) protein, a single-chain transmembrane glycoprotein, plays an important role in immune adherence and clearing complement-opsonized immune complexes. Here, the mRNA of the porcine primate-like complement receptor (CR1-like) gene was analyzed, and two domain sequences with potential functions were cloned into the pwPICZalpha vector for expression in Pichia pastoris. The recombinant proteins were purified with both Protein Pure Ni-NTA resin and strong anion exchange resin. The activities of the purified recombinant proteins were evaluated by SDS-PAGE, western blotting, and complement receptor assays. The results indicated that two domains of the CR1-like protein, CCP36 and CCP811 with molecular weights of 29.8 kDa and 30 kDa, respectively, were successfully expressed in P. pastoris. These two recombinant proteins possess some of the functions of the primate CR1 protein. Using these two proteins coupled with an antibody blocking technique, we also showed that CR1-like is expressed on natural porcine erythrocytes.

journal_name

Immunol Res

journal_title

Immunologic research

authors

Yin W,Wei X,Jiang J,Fan K,Zhao J,Sun N,Wang Z,Sun Y,Ma H,Zhao X,Li H

doi

10.1007/s12026-016-8792-z

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

1025-32

issue

4

eissn

0257-277X

issn

1559-0755

pii

10.1007/s12026-016-8792-z

journal_volume

64

pub_type

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