Abstract:
:Here we aimed to develop a capillary electrophoresis-based high-throughput multiplex polymerase chain reaction (PCR) system for the simultaneous detection of nine pathogens in swine. Nine pairs of specific primers and a set of universal primers were designed; the multiplex PCR was established. The specificity and cross-reactivity of this assay were examined, and the detection limit was determined using serial 10-fold dilutions of plasmids containing the target sequences. The assay was further tested using 144 clinical samples. We found that the nine specific amplification peaks were observed, and the assay had a high degree of specificity, without nonspecific amplification. The simultaneous detection limit for the nine viruses reached 10000 copies μL-1 when all of the premixed viral targets were present. Seventy-seven of the clinical samples tested positive for at least one of the viruses; the principal viral infections in the clinical samples were porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus. This approach has much potential for further development of high-throughput detection tools for the diagnosis of diseases in animals.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Wu XL,Xiao L,Lin H,Yang M,Chen SJ,An W,Wang Y,Yao XP,Yang ZX,Tang ZZdoi
10.1155/2017/7243909subject
Has Abstractpub_date
2017-01-01 00:00:00pages
7243909eissn
2314-6133issn
2314-6141journal_volume
2017pub_type
杂志文章abstract::The 13 million nurses worldwide constitute most of the global healthcare workforce and are uniquely positioned to engage with others to address disparities in healthcare to achieve the goal of better health for all. A new vision for nurses involves active participation and collaboration with international colleagues a...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2016/3127543
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journal_title:BioMed research international
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doi:10.1155/2013/734326
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
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abstract:BACKGROUND:This study aimed to compare the effects of rectal midazolam addition after applying bupivacaine and caudal anesthesia on postoperative analgesia time, the need for additional analgesics, postoperative recovery, and sedation and to find out its adverse effects in children having lower abdominal surgery. METH...
journal_title:BioMed research international
pub_type: 杂志文章,随机对照试验
doi:10.1155/2014/127548
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/4816527
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/8398156
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journal_title:BioMed research international
pub_type: 杂志文章,随机对照试验
doi:10.1155/2015/683640
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doi:10.1155/2016/6040146
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
doi:10.1155/2013/407263
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doi:10.1155/2015/972481
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journal_title:BioMed research international
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doi:10.1155/2018/8273581
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journal_title:BioMed research international
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doi:10.1155/2014/609086
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/4094191
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1742326
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2013/516427
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journal_title:BioMed research international
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doi:10.1155/2014/827415
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abstract::We test the hypothesis that ultrasound-targeted microbubble destruction (UTMD) technique increases the renoprotective effect of kidney-targeted transplantation of bone-marrow-derived mesenchymal stem cells (BM-MSCs) in diabetic nephropathy (DN) rats. Diabetes was induced by streptozotocin injection (60 mg/Kg, intraper...
journal_title:BioMed research international
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doi:10.1155/2013/526367
更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1155/2015/905215
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/805876
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/941831
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