Photobiomodulation in the Metabolism of Lipopolysaccharides-exposed Epithelial Cells and Gingival Fibroblasts.

Abstract:

:This study assessed the effects of photobiomodulation (PBM) to cells previously exposed to lipopolysaccharides (LPS). Human gingival fibroblasts (HGF) and epithelial cells (HaCaT) were seeded in wells of 24-well plates containing complete culture medium (DMEM). After 24 h, the DMEM was replaced by serum-free DMEM, and cells were exposed to LPS of Escherichia coli (E. coli) (10 μg mL-1 ) for 24, 48, and 72 h. The cells were subjected to specific parameters of phototherapy (PT) (LASERTable-InGaAsP-780 ± 3 nm, 25 mW, 3 J cm-2 ). Cell proliferation (alamarBlue® ), viability (Trypan Blue) and synthesis of CCL2 (ELISA) were evaluated. Data were statistically analyzed by the Kruskal-Wallis and Mann-Whitney test (α = 5%). Proliferation and viability of both cell lines decreased after LPS treatment at 48 and 72 h. Enhanced synthesis of CCL2 by gingival fibroblasts occurred at 24 h, while epithelial cells increased synthesis of this chemokine at 48 and 72 h. PBM enhanced cell proliferation and viability in a time-dependent manner for both cell lines exposed or not to LPS, while synthesis of CCL2 by cells exposed to PT decreased over time. PBM caused biomodulatory effects on gingival fibroblasts and epithelial cells previously treated with LPS. These effects may decrease tissue inflammatory response and accelerate wound healing of oral mucosal tissue.

journal_name

Photochem Photobiol

authors

Pansani TN,Basso FG,Soares DG,Turrioni APDS,Hebling J,de Souza Costa CA

doi

10.1111/php.12877

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

598-603

issue

3

eissn

0031-8655

issn

1751-1097

journal_volume

94

pub_type

杂志文章
  • Interaction of retinoic acid radical cation with lysozyme and antioxidants: laser flash photolysis study in microemulsion.

    abstract::All-trans retinoic acid (ATRA) plays essential roles in the normal biological processes and the treatment of cancer and skin diseases. Considering its photosensitive property, many studies have been focused on the photochemistry of ATRA. In this study, we investigated the transient phenomena in the laser flash photoly...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12128

    authors: Li K,Wang M,Wang T,Sun D,Zhu R,Sun X,Wu X,Wang SL

    更新日期:2013-09-01 00:00:00

  • Larval and adult emission spectra of bioluminescence in three European firefly species.

    abstract::We studied the spectral characteristics of the larvae of three sympatric Belgian species of fireflies, Lampyris noctiluca, Phosphaenus hemipterus and Lamprohiza splendidula. An in vivo spectral study was performed to compare bioluminescence spectra. The emission spectrum of a laboratory reared female L. noctiluca was ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2003-11-11-RA.1

    authors: De Cock R

    更新日期:2004-04-01 00:00:00

  • Metformin, an antidiabetic agent reduces growth of cutaneous squamous cell carcinoma by targeting mTOR signaling pathway.

    abstract::The biguanide metformin is widely used for the treatment of Type-II diabetes. Its antiproliferative and pro-apoptotic effects in various tumor cells suggest its potential candidacy for cancer chemoprevention. Herein, we report that metformin significantly inhibited human epidermoid A431 tumor xenograft growth in nu/nu...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2012.01165.x

    authors: Chaudhary SC,Kurundkar D,Elmets CA,Kopelovich L,Athar M

    更新日期:2012-09-01 00:00:00

  • Hypocrellin A photosensitization involves an intracellular pH decrease in 3T3 cells.

    abstract::The fluorescent pH probe carboxy-seminaphtorhodafluor-1 (C-Snarf-1) has been used for laser microspectro-fluorometric assays of intracellular pH in 3T3 mouse fibroblasts treated with hypocrellin A. These results are compared to those previously obtained with the structurally related hydroxylated polycyclic quinone, hy...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Chaloupka R,Sureau F,Kocisova E,Petrich JW

    更新日期:1998-07-01 00:00:00

  • Daily Maximum Erythemal Dose Rates in the Tropics.

    abstract::The maximum erythemal dose rate (EDRmax ) at the Earth's surface tends to occur at local noon. However, clouds can make the timing of EDRmax significantly away from local noon. In fact, EDRmax and its time of occurrence depend mainly on the solar zenith angle at noon (SZAn ), site's altitude, the total ozone column (T...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13054

    authors: Silva AA,Yamamoto ALC,Corrêa MP

    更新日期:2019-05-01 00:00:00

  • Characterization of endogenous protoporphyrin IX induced by delta-aminolevulinic acid in resting and activated peripheral blood lymphocytes by four-color flow cytometry.

    abstract::Lymphocytes treated with delta-aminolevulinic acid (ALA) can accumulate the photoactive, fluorescent heme precursor, protoporphyrin IX (PpIX). With visible light illumination, PpIX can be used in photodynamic therapy (ALA-PDT) to kill or functionally alter cells. The aim of this study was to characterize the effects o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Hryhorenko EA,Rittenhouse-Diakun K,Harvey NS,Morgan J,Stewart CC,Oseroff AR

    更新日期:1998-05-01 00:00:00

  • Water-mediated spectral shifts in rhodopsin and bathorhodopsin.

    abstract::The role of water molecules in spectral tuning of proteins has been left largely unexplored. This topic is important because changing hydrogen bond patterns during the activation process may lead to spectral shifts which can be of diagnostic value for the underlying structures. Arguments put forward in this article ar...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1751-1097.2008.00499.x

    authors: Sekharan S

    更新日期:2009-03-01 00:00:00

  • The distance between the phytochrome chromophore and the N-terminal chain decreases during phototransformation. A novel fluorescence energy transfer method using labeled antibody fragments.

    abstract::A novel antibody-fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N-terminal bound, fluorescently labeled Oat-25 Fab antibody fragments to the phytochrome chromophore was measured. The results sugge...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1992.tb02227.x

    authors: Farrens DL,Cordonnier MM,Pratt LH,Song PS

    更新日期:1992-11-01 00:00:00

  • Type I and Type II Photosensitized Oxidation Reactions: Guidelines and Mechanistic Pathways.

    abstract::Here, 10 guidelines are presented for a standardized definition of type I and type II photosensitized oxidation reactions. Because of varied notions of reactions mediated by photosensitizers, a checklist of recommendations is provided for their definitions. Type I and type II photoreactions are oxygen-dependent and in...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12716

    authors: Baptista MS,Cadet J,Di Mascio P,Ghogare AA,Greer A,Hamblin MR,Lorente C,Nunez SC,Ribeiro MS,Thomas AH,Vignoni M,Yoshimura TM

    更新日期:2017-07-01 00:00:00

  • p53 Mutations in hairless SKH-hr1 mouse skin tumors induced by a solar simulator.

    abstract::In this study, we investigated whether the spectrum of p53 mutations in skin tumors induced in hairless SKH-hr1 mice by a solar simulator (290-400 nm) are similar to those found in skin tumors induced in C3H mice by UV radiation from unfiltered (250-400 nm) and Kodacel-filtered (290-400 nm) FS40 sunlamps. Analysis of ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(1998)067<0227:mihshm>2.3.co;2

    authors: Ananthaswamy HN,Fourtanier A,Evans RL,Tison S,Medaisko C,Ullrich SE,Kripke ML

    更新日期:1998-02-01 00:00:00

  • Subcellular distribution of hypericin in human cancer cells.

    abstract::Confocal laser microspectrofluorometric measurements on human T47D mammary tumor cells have been performed to assess the intracellular distribution of hypericin within the various cell compartments: cytoplasmic membrane, cytoplasm and nucleus. Confocal fluorescence measurements obtained from microvolumes (approximatel...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1995.tb02382.x

    authors: Miskovsky P,Sureau F,Chinsky L,Turpin PY

    更新日期:1995-09-01 00:00:00

  • Chlorin e6 derivative radachlorin mainly accumulates in mitochondria, lysosome and endoplasmic reticulum and shows high affinity toward tumors in nude mice in photodynamic therapy.

    abstract::The efficacy of photodynamic therapy (PDT) depends upon the amount of photosensitizer accumulated in the malignant tissues. Radachlorin is a popular photosensitizer used in photodynamic therapy to treat various types of cancer. In this study, we have studied the main organelles responsible for the accumulation of rada...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12273

    authors: Biswas R,Moon JH,Ahn JC

    更新日期:2014-09-01 00:00:00

  • Molecular mechanisms of ultraviolet radiation carcinogenesis.

    abstract::UV radiation is a potent DNA damaging agent and a known inducer of skin cancer in experimental animals. There is excellent scientific evidence to indicate that most non-melanoma human skin cancers are induced by repeated exposure to sunlight. UV radiation is unique in that it induces DNA damage that differs from the l...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1751-1097.1990.tb08452.x

    authors: Ananthaswamy HN,Pierceall WE

    更新日期:1990-12-01 00:00:00

  • Role of nuclear factor-kappa B in colon cancer cell apoptosis mediated by aminopyropheophorbide photosensitization.

    abstract::Aminopyropheophorbide (APP) is a second generation of photosensitizer for photodynamic therapy (PDT). We demonstrated that APP strongly absorbed red light and, after being taken up by colon cancer cells (HCT-116 cells), was localized in cytoplasmic and internal membranes but not in mitochondria. The APP-mediated photo...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Matroule JY,Hellin AC,Morlière P,Fabiano AS,Santus R,Merville MP,Piette J

    更新日期:1999-10-01 00:00:00

  • General purpose lamps induce polyoma DNA replication in H3 cells.

    abstract::We have previously demonstrated the ability of UVC (254 nm) radiation to induce asynchronous polyoma replication in rat fibroblast cells (H3 line) that contain an integrated copy of polyoma virus. In the present study we show that general purpose lamps can induce polyoma replication in these cells as well. The amount ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1993.tb09559.x

    authors: Rutberg SE,Ronai Z

    更新日期:1993-08-01 00:00:00

  • Formation of aggregates of tryptic fragments derived from the carboxyl-terminal half of pea phytochrome and localization of the site of contact between the fragments by amino-terminal amino acid sequence analysis.

    abstract::Limited trypsinization of large pea (Pisum sativum cv. Alaska) phytochrome and subsequent size-exclusion chromatography (SEC) in 0.1 M Na phosphate, pH 7.8, yielded a high-molecular-mass aggregate of tryptic fragments of phytochrome. Further SEC in 0.1 M Tris-HCl, pH 7.5, plus various concentrations of NaSCN, indicate...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1989.tb04136.x

    authors: Yamamoto KT,Tokutomi S

    更新日期:1989-07-01 00:00:00

  • Isolation and characteristics of the PSI-LHCI-LHCII supercomplex under high light.

    abstract::We developed a novel method for the isolation of the PSI-LHCI-LHCII complex from spinach leaves. The supercomplex was resolved into a core complex (CPI), LHCII trimers, LHCI dimers and LHCII monomers using green gel electrophoresis. We then investigate changes in the fluorescence and absorption spectra of PSI-LHCI-LHC...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2010.00830.x

    authors: Qin X,Wang W,Wang K,Xin Y,Kuang T

    更新日期:2011-01-01 00:00:00

  • Protective effects of cyclooxygenase-2 inhibitors on narrow-band ultraviolet B-irradiated epidermal Ia+ Langerhans cells and Thy-1+ dendritic epidermal T cells in mice.

    abstract::Cyclooxygenase (COX)-2 inhibitors are known to be used as chemopreventative agents against certain malignancies. Thus far, there has been very limited information on whether COX-2 inhibitors protect against chronic narrow-band UVB (NB-UVB)-induced immunosuppression. The present study investigated the effect of nonsele...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2007.00289.x

    authors: Wu Y,Tai CL,Wei H,He F,Wang Y,Zhao Y,Chen HD

    更新日期:2008-03-01 00:00:00

  • Roles of porphyrin and iron metabolisms in the δ-aminolevulinic acid (ALA)-induced accumulation of protoporphyrin and photodamage of tumor cells.

    abstract::δ-Aminolevulinic acid (ALA)-induced porphyrin accumulation is widely used in the treatment of cancer, as photodynamic therapy. To clarify the mechanisms of the tumor-preferential accumulation of protoporphyrin, we examined the effect of the expression of heme-biosynthetic and -degradative enzymes on the ALA-induced ac...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2011.00950.x

    authors: Ohgari Y,Miyata Y,Miyagi T,Gotoh S,Ohta T,Kataoka T,Furuyama K,Taketani S

    更新日期:2011-09-01 00:00:00

  • Photosensitizers in dermatology.

    abstract::Systemic injection of hematoporphyrin derivative (HpD) in contribution with visible light (red or blue-green) delivered by laser was used to treat a patient with psoriasis. The psoriatic lesions responded vigorously to laser treatments, forming eschars by 1 week post irradiation. In contrast, only minimal erythema was...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1987.tb04739.x

    authors: McCullough JL,Weinstein GD,Douglas JL,Berns MW

    更新日期:1987-07-01 00:00:00

  • Behaviors producing photodispersal in Stentor coeruleus.

    abstract::The ciliate Stentor coeruleus exhibits photodispersal, that is, these cells swim away from light sources and collect in dimly lighted areas. We imaged and reconstructed the tracks of 48 Stentor to determine which swimming behaviors produced their photodispersal. We observed that their photodispersal is not due to a ch...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2004)080<0401:BPPISC>2.0.CO;2

    authors: Menzies E,Das N,Wood DC

    更新日期:2004-11-01 00:00:00

  • Psoralen Derivatives with Enhanced Potency.

    abstract::Psoralen is a furocoumarin natural product that intercalates within DNA and forms covalent adducts when activated by ultraviolet radiation. It is well known that this property contributes to psoralen's clinical efficacy in several disease contexts, which include vitiligo, psoriasis, graft-versus-host disease and cutan...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13263

    authors: Buhimschi AD,Gooden DM,Jing H,Fels DR,Hansen KS,Beyer WF Jr,Dewhirst MW,Walder H,Gasparro FP

    更新日期:2020-09-01 00:00:00

  • Cancer Cell-targeted and Activatable Photoimmunotherapy Spares T Cells in a 3D Coculture Model.

    abstract::Photodynamic therapy (PDT) is an established therapeutic modality that uses nonionizing near-infrared light to activate photocytotoxicity of endogenous or exogenous photosensitizers (PSs). An ongoing avenue of cancer research involves leveraging PDT to stimulate antitumor immune responses; however, these effects appea...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13153

    authors: Kercher EM,Nath S,Rizvi I,Spring BQ

    更新日期:2020-03-01 00:00:00

  • Photodynamic therapy with topical delta-aminolevulinic acid delays UV photocarcinogenesis in hairless mice.

    abstract::Photodynamic therapy (PDT) with topical application of delta-aminolevulinic acid (ALA) followed by irradiation with visible light (ALA-PDT) is a relatively new and promising experimental treatment of superficial premalignant and malignant skin neoplasms. The purpose of this study was to determine whether ALA-PDT can p...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1997.tb03178.x

    authors: Stender IM,Bech-Thomsen N,Poulsen T,Wulf HC

    更新日期:1997-10-01 00:00:00

  • Interaction between human alpha1-acid glycoprotein (orosomucoid) and 2-p-toluidinylnaphthalene-6-sulfonate.

    abstract::The interaction between human alpha1-acid glycoprotein (orosomucoid) and the fluorescent probe, 2-p-toluidinylnaphthalene-6-sulfonate (TNS) has been studied. An association constant of 16.7 (+/- 3) x 10(3) M-1 was obtained for the complex at 20 degrees C with a stoichiometry of 1:1. From the effect of temperature on t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1995.tb05234.x

    authors: Albani J,Vos R,Willaert K,Engelborghs Y

    更新日期:1995-07-01 00:00:00

  • Correlations between reported and measured ultraviolet radiation exposure of mothers and young children.

    abstract::Cumulative and intermittent sun exposure are risk factors for skin cancer, highlighting the need to monitor exposure during childhood. There is currently very little available information concerning the accuracy of self-reported levels of sun exposure, particularly for very young children. In this study, UV radiation ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0060:cbramu>2.0.co;2

    authors: O'Riordan DL,Stanton WR,Eyeson-Annan M,Gies P,Roy C

    更新日期:2000-01-01 00:00:00

  • Diastereoselectivity and site dependency in the photochemistry of ketoprofen in the bovine serum albumin matrix.

    abstract::The photodegradation of the S(+)- and R(-)-ketoprofen (KP) enantiomers in the bovine serum albumin matrix was studied by steady-state photolysis with the use of lambda(irr) > 320 nm and transient absorption spectroscopy with lambda(exc) = 355 nm, at 1/1 and 2/1 KP/BSA molar ratios. R(-)-KP was found to be more labile ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-07-11-RA-608

    authors: Monti S,Manet I,Manoli F,Morrone R,Nicolosi G,Sortino S

    更新日期:2006-01-01 00:00:00

  • Continuous noninvasive measurement of in vivo pH in conscious mice.

    abstract::Extracellular pH was measured continuously and noninvasively in hairless mice using a transcutaneous spectrofluorometric technique and a fluorescent pH probe, bis-carboxyethyl carboxyfluorescein (BCECF). The acid form of BCECF was injected intravenously. An optical fiber system excited the fluorescent probe at 450 and...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1994.tb05039.x

    authors: Russell DA,Pottier RH,Valenzeno DP

    更新日期:1994-03-01 00:00:00

  • Secondary Dark Reactions Following Photodynamic Treatment are More Damaging Than Previously Thought.

    abstract::Photodynamic treatment is often thought to produce reactive oxygen species (ROS) that directly induce killing; the nomenclature and phrases revolve around such notions of light-dependency. Few studies reference the possible existence of oxidation products formed in secondary reactions, which bear cytotoxicity competit...

    journal_title:Photochemistry and photobiology

    pub_type: 评论,杂志文章

    doi:10.1111/php.12984

    authors: Chiemezie C,Greer A

    更新日期:2019-01-01 00:00:00

  • Quantum efficiencies of the reversible photoreaction of octopus rhodopsin.

    abstract::The classic method of photometric curves for photosensitivity determination has been extended to the case of photoreversible reactions and applied to the octopus rhodopsin --> acid metarhodopsin photoreaction. In such cases, measurements at one irradiation wavelength yield the sum of the photosensitivities of the forw...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1987.tb04744.x

    authors: Dixon SF,Cooper A

    更新日期:1987-07-01 00:00:00