Abstract:
:This study assessed the effects of photobiomodulation (PBM) to cells previously exposed to lipopolysaccharides (LPS). Human gingival fibroblasts (HGF) and epithelial cells (HaCaT) were seeded in wells of 24-well plates containing complete culture medium (DMEM). After 24 h, the DMEM was replaced by serum-free DMEM, and cells were exposed to LPS of Escherichia coli (E. coli) (10 μg mL-1 ) for 24, 48, and 72 h. The cells were subjected to specific parameters of phototherapy (PT) (LASERTable-InGaAsP-780 ± 3 nm, 25 mW, 3 J cm-2 ). Cell proliferation (alamarBlue® ), viability (Trypan Blue) and synthesis of CCL2 (ELISA) were evaluated. Data were statistically analyzed by the Kruskal-Wallis and Mann-Whitney test (α = 5%). Proliferation and viability of both cell lines decreased after LPS treatment at 48 and 72 h. Enhanced synthesis of CCL2 by gingival fibroblasts occurred at 24 h, while epithelial cells increased synthesis of this chemokine at 48 and 72 h. PBM enhanced cell proliferation and viability in a time-dependent manner for both cell lines exposed or not to LPS, while synthesis of CCL2 by cells exposed to PT decreased over time. PBM caused biomodulatory effects on gingival fibroblasts and epithelial cells previously treated with LPS. These effects may decrease tissue inflammatory response and accelerate wound healing of oral mucosal tissue.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Pansani TN,Basso FG,Soares DG,Turrioni APDS,Hebling J,de Souza Costa CAdoi
10.1111/php.12877subject
Has Abstractpub_date
2018-05-01 00:00:00pages
598-603issue
3eissn
0031-8655issn
1751-1097journal_volume
94pub_type
杂志文章abstract::All-trans retinoic acid (ATRA) plays essential roles in the normal biological processes and the treatment of cancer and skin diseases. Considering its photosensitive property, many studies have been focused on the photochemistry of ATRA. In this study, we investigated the transient phenomena in the laser flash photoly...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12128
更新日期:2013-09-01 00:00:00
abstract::We studied the spectral characteristics of the larvae of three sympatric Belgian species of fireflies, Lampyris noctiluca, Phosphaenus hemipterus and Lamprohiza splendidula. An in vivo spectral study was performed to compare bioluminescence spectra. The emission spectrum of a laboratory reared female L. noctiluca was ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2003-11-11-RA.1
更新日期:2004-04-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01165.x
更新日期:2012-09-01 00:00:00
abstract::The fluorescent pH probe carboxy-seminaphtorhodafluor-1 (C-Snarf-1) has been used for laser microspectro-fluorometric assays of intracellular pH in 3T3 mouse fibroblasts treated with hypocrellin A. These results are compared to those previously obtained with the structurally related hydroxylated polycyclic quinone, hy...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::The maximum erythemal dose rate (EDRmax ) at the Earth's surface tends to occur at local noon. However, clouds can make the timing of EDRmax significantly away from local noon. In fact, EDRmax and its time of occurrence depend mainly on the solar zenith angle at noon (SZAn ), site's altitude, the total ozone column (T...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13054
更新日期:2019-05-01 00:00:00
abstract::Lymphocytes treated with delta-aminolevulinic acid (ALA) can accumulate the photoactive, fluorescent heme precursor, protoporphyrin IX (PpIX). With visible light illumination, PpIX can be used in photodynamic therapy (ALA-PDT) to kill or functionally alter cells. The aim of this study was to characterize the effects o...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::The role of water molecules in spectral tuning of proteins has been left largely unexplored. This topic is important because changing hydrogen bond patterns during the activation process may lead to spectral shifts which can be of diagnostic value for the underlying structures. Arguments put forward in this article ar...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
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更新日期:2009-03-01 00:00:00
abstract::A novel antibody-fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N-terminal bound, fluorescently labeled Oat-25 Fab antibody fragments to the phytochrome chromophore was measured. The results sugge...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1992.tb02227.x
更新日期:1992-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.12716
更新日期:2017-07-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(1998)067<0227:mihshm>2.3.co;2
更新日期:1998-02-01 00:00:00
abstract::Confocal laser microspectrofluorometric measurements on human T47D mammary tumor cells have been performed to assess the intracellular distribution of hypericin within the various cell compartments: cytoplasmic membrane, cytoplasm and nucleus. Confocal fluorescence measurements obtained from microvolumes (approximatel...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb02382.x
更新日期:1995-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12273
更新日期:2014-09-01 00:00:00
abstract::UV radiation is a potent DNA damaging agent and a known inducer of skin cancer in experimental animals. There is excellent scientific evidence to indicate that most non-melanoma human skin cancers are induced by repeated exposure to sunlight. UV radiation is unique in that it induces DNA damage that differs from the l...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/j.1751-1097.1990.tb08452.x
更新日期:1990-12-01 00:00:00
abstract::Aminopyropheophorbide (APP) is a second generation of photosensitizer for photodynamic therapy (PDT). We demonstrated that APP strongly absorbed red light and, after being taken up by colon cancer cells (HCT-116 cells), was localized in cytoplasmic and internal membranes but not in mitochondria. The APP-mediated photo...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::We have previously demonstrated the ability of UVC (254 nm) radiation to induce asynchronous polyoma replication in rat fibroblast cells (H3 line) that contain an integrated copy of polyoma virus. In the present study we show that general purpose lamps can induce polyoma replication in these cells as well. The amount ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb09559.x
更新日期:1993-08-01 00:00:00
abstract::Limited trypsinization of large pea (Pisum sativum cv. Alaska) phytochrome and subsequent size-exclusion chromatography (SEC) in 0.1 M Na phosphate, pH 7.8, yielded a high-molecular-mass aggregate of tryptic fragments of phytochrome. Further SEC in 0.1 M Tris-HCl, pH 7.5, plus various concentrations of NaSCN, indicate...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1989.tb04136.x
更新日期:1989-07-01 00:00:00
abstract::We developed a novel method for the isolation of the PSI-LHCI-LHCII complex from spinach leaves. The supercomplex was resolved into a core complex (CPI), LHCII trimers, LHCI dimers and LHCII monomers using green gel electrophoresis. We then investigate changes in the fluorescence and absorption spectra of PSI-LHCI-LHC...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00830.x
更新日期:2011-01-01 00:00:00
abstract::Cyclooxygenase (COX)-2 inhibitors are known to be used as chemopreventative agents against certain malignancies. Thus far, there has been very limited information on whether COX-2 inhibitors protect against chronic narrow-band UVB (NB-UVB)-induced immunosuppression. The present study investigated the effect of nonsele...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2007.00289.x
更新日期:2008-03-01 00:00:00
abstract::δ-Aminolevulinic acid (ALA)-induced porphyrin accumulation is widely used in the treatment of cancer, as photodynamic therapy. To clarify the mechanisms of the tumor-preferential accumulation of protoporphyrin, we examined the effect of the expression of heme-biosynthetic and -degradative enzymes on the ALA-induced ac...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2011.00950.x
更新日期:2011-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1987.tb04739.x
更新日期:1987-07-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2004)080<0401:BPPISC>2.0.CO;2
更新日期:2004-11-01 00:00:00
abstract::Psoralen is a furocoumarin natural product that intercalates within DNA and forms covalent adducts when activated by ultraviolet radiation. It is well known that this property contributes to psoralen's clinical efficacy in several disease contexts, which include vitiligo, psoriasis, graft-versus-host disease and cutan...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13263
更新日期:2020-09-01 00:00:00
abstract::Photodynamic therapy (PDT) is an established therapeutic modality that uses nonionizing near-infrared light to activate photocytotoxicity of endogenous or exogenous photosensitizers (PSs). An ongoing avenue of cancer research involves leveraging PDT to stimulate antitumor immune responses; however, these effects appea...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13153
更新日期:2020-03-01 00:00:00
abstract::Photodynamic therapy (PDT) with topical application of delta-aminolevulinic acid (ALA) followed by irradiation with visible light (ALA-PDT) is a relatively new and promising experimental treatment of superficial premalignant and malignant skin neoplasms. The purpose of this study was to determine whether ALA-PDT can p...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1997.tb03178.x
更新日期:1997-10-01 00:00:00
abstract::The interaction between human alpha1-acid glycoprotein (orosomucoid) and the fluorescent probe, 2-p-toluidinylnaphthalene-6-sulfonate (TNS) has been studied. An association constant of 16.7 (+/- 3) x 10(3) M-1 was obtained for the complex at 20 degrees C with a stoichiometry of 1:1. From the effect of temperature on t...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb05234.x
更新日期:1995-07-01 00:00:00
abstract::Cumulative and intermittent sun exposure are risk factors for skin cancer, highlighting the need to monitor exposure during childhood. There is currently very little available information concerning the accuracy of self-reported levels of sun exposure, particularly for very young children. In this study, UV radiation ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)071<0060:cbramu>2.0.co;2
更新日期:2000-01-01 00:00:00
abstract::The photodegradation of the S(+)- and R(-)-ketoprofen (KP) enantiomers in the bovine serum albumin matrix was studied by steady-state photolysis with the use of lambda(irr) > 320 nm and transient absorption spectroscopy with lambda(exc) = 355 nm, at 1/1 and 2/1 KP/BSA molar ratios. R(-)-KP was found to be more labile ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-07-11-RA-608
更新日期:2006-01-01 00:00:00
abstract::Extracellular pH was measured continuously and noninvasively in hairless mice using a transcutaneous spectrofluorometric technique and a fluorescent pH probe, bis-carboxyethyl carboxyfluorescein (BCECF). The acid form of BCECF was injected intravenously. An optical fiber system excited the fluorescent probe at 450 and...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1994.tb05039.x
更新日期:1994-03-01 00:00:00
abstract::Photodynamic treatment is often thought to produce reactive oxygen species (ROS) that directly induce killing; the nomenclature and phrases revolve around such notions of light-dependency. Few studies reference the possible existence of oxidation products formed in secondary reactions, which bear cytotoxicity competit...
journal_title:Photochemistry and photobiology
pub_type: 评论,杂志文章
doi:10.1111/php.12984
更新日期:2019-01-01 00:00:00
abstract::The classic method of photometric curves for photosensitivity determination has been extended to the case of photoreversible reactions and applied to the octopus rhodopsin --> acid metarhodopsin photoreaction. In such cases, measurements at one irradiation wavelength yield the sum of the photosensitivities of the forw...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1987.tb04744.x
更新日期:1987-07-01 00:00:00