Interaction between human alpha1-acid glycoprotein (orosomucoid) and 2-p-toluidinylnaphthalene-6-sulfonate.


:The interaction between human alpha1-acid glycoprotein (orosomucoid) and the fluorescent probe, 2-p-toluidinylnaphthalene-6-sulfonate (TNS) has been studied. An association constant of 16.7 (+/- 3) x 10(3) M-1 was obtained for the complex at 20 degrees C with a stoichiometry of 1:1. From the effect of temperature on the binding process, the standard enthalpy change for the binding is calculated to be delta H0 = -18 +/- 3 kJ mol-1 and the standard entropy change delta S0 = 19 +/- 12 J K-1 mol-1. The tryptophan fluorescence of the protein can be described by a sum of three exponentials. Upon TNS binding, the average fluorescence lifetime of the protein in the complex changes much less than the fluorescence intensity. The bound TNS is therefore a very efficient acceptor for the protein fluorescence. The TNS bound to orosomucoid present two fluorescence lifetimes 11 and 4.3 ns. The possible origins of the two lifetimes are discussed.


Photochem Photobiol


Albani J,Vos R,Willaert K,Engelborghs Y




Has Abstract


1995-07-01 00:00:00












  • Fluorescence lifetimes of protochlorophyllide in plants with different proportions of short-wavelength and long-wavelength protochlorophyllide spectral forms.

    abstract::Dark-grown leaves of maize (Zea mays), wheat (Triticum aestivum), wild-type pea (Pisum sativum) and its light-independent photomorphogenesis mutant (lip1) have different proportions of protochlorophyllide (Pchlide) forms as revealed by low-temperature fluorescence emission spectra. Four discrete spectral forms of Pchl...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Myśliwa-Kurdziel B,Amirjani MR,Strzałka K,Sundqvist C

    更新日期:2003-08-01 00:00:00

  • Oxidation of human catalase by singlet oxygen in myeloid leukemia cells.

    abstract::Catalases are oxidized by singlet oxygen giving rise to more acidic conformers detected in zymograms after electrophoresis in polyacrylamide gels. This shift in catalase mobility can be indicative of singlet oxygen production in vivo. Catalase from human cells, as from many organisms, is susceptible to in vitro modifi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Lledías F,Hansberg W

    更新日期:1999-12-01 00:00:00

  • Daily duration of vitamin D synthesis in human skin with relation to latitude, total ozone, altitude, ground cover, aerosols and cloud thickness.

    abstract::Vitamin D production in human skin occurs only when incident UV radiation exceeds a certain threshold. From simulations of UV irradiances worldwide and throughout the year, we have studied the dependency of the extent and duration of cutaneous vitamin D production in terms of latitude, time, total ozone, clouds, aeros...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Engelsen O,Brustad M,Aksnes L,Lund E

    更新日期:2005-11-01 00:00:00

  • Formation of protein-bound 3,4-dihydroxyphenylalanine in collagen types I and IV exposed to ultraviolet light.

    abstract::Collagen was exposed to an ultraviolet (UV) lamp that emitted predominantly in the UVB range. The cross-linking of collagen type I and type IV by UV irradiation was observed. Amino acid analyses revealed that Tyr residues in both collagen types I and IV were decreased by irradiation. In collagen type IV, losses of His...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Kato Y,Nishikawa T,Kawakishi S

    更新日期:1995-04-01 00:00:00

  • Iodouracil as a personal dosimeter for solar UVB.

    abstract::A solution of iodouracil (0.2 mg/mL) and KI (0.1 M) in 0.23 M borate buffer (pH 7) forms triiodide upon exposure to UVB. In the presence of thyodene, the blue starch-iodine complex is formed, the intensity of which is proportional to the amount of UVB exposure. Studies were conducted using either a sunlamp or solar ir...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Rahn R,Lee MA

    更新日期:1998-08-01 00:00:00

  • Photodegradation and in vitro phototoxicity of fenofibrate, a photosensitizing anti-hyperlipoproteinemic drug.

    abstract::The phototoxic anti-hyperlipoproteinemic drug fenofibrate was found to be photolabile under aerobic and anaerobic conditions. Irradiation under argon of a methanol solution of this drug produced the photoproducts isopropyl 4-(1-[4-chlorophenyl]-1,2-dihydroxy)ethylphenoxyisobutyrate, 1,2-bis(4-chlorophenyl)-1,2bis (4-[...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Vargas F,Canudas N,Miranda MA,Boscá F

    更新日期:1993-10-01 00:00:00

  • Sub-lethal photodynamic damage to ARPE-19 cells transiently inhibits their phagocytic activity.

    abstract::Efficient phagocytosis of photoreceptor outer segments (POS) membranes by retinal pigment epithelium (RPE) plays a key role in biological renewal of these highly peroxidizable structures. Here, we tested whether photodynamic treatment, mediated by merocyanine 540 (MC 540), rose Bengal or a zinc-substituted chlorophyll...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Olchawa M,Szewczyk G,Zareba M,Piłat A,Bzowska M,Mikołajczyk T,Sarna T

    更新日期:2010-07-01 00:00:00

  • Photosynthetic Performance of the Red Alga Pyropia haitanensis During Emersion, With Special Reference to Effects of Solar UV Radiation, Dehydration and Elevated CO2 Concentration.

    abstract::Macroalgae distributed in intertidal zones experience a series of environmental changes, such as periodical desiccation associated with tidal cycles, increasing CO2 concentration and solar UVB (280-315 nm) irradiance in the context of climate change. We investigated how the economic red macroalga, Pyropia haitanensis,...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Xu J,Gao K

    更新日期:2015-11-01 00:00:00

  • Water and carboxyl group environments in the dehydration blueshift of bacteriorhodopsin.

    abstract::The proton channels of the bacteriorhodopsin (BR) proton pump contain bound water molecules. The channels connect the purple membrane surfaces with the protonated retinal Schiff base at the membrane center. Films of purple membrane equilibrated at low relative humidity display a shift of the 570 nm retinal absorbance ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Renthal R,Gracia N,Regalado R

    更新日期:2000-11-01 00:00:00

  • Comparison of photosensitizer (AIPcS2) quantification techniques: in situ fluorescence microsampling versus tissue chemical extraction.

    abstract::A noninvasive in situ fluorescence-based method for the quantification of the photosensitizer chloroaluminum disulfonated phthalocyanine was compared to the highly accurate but nonreal time ex vivo spectrofluorometry method. Our in vivo fluorescence technique is designed to allow real-time assessment of photosensitize...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Lee CC,Pouge BW,Strawbridge RR,Moodie KL,Bartholomew LR,Burke GC,Hoopes PJ

    更新日期:2001-09-01 00:00:00

  • Axially Decorated SiIV -phthalocyanines Bearing Mannose- or Ammonium-conjugated Siloxanes: Comparative Bacterial Labeling and Photodynamic Inactivation.

    abstract::Herein, we present a comparative study about the photoinactivation of Staphylococcus aureus (Gram-positive model) and Escherichia coli (Gram-negative model) employing a neutral and a dicationic axially functionalized SiIV -phthalocyanine. Depending on the charge of the siloxane moiety (neutral monosaccharide or cation...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Grüner MC,Niemann S,Faust A,Strassert CA

    更新日期:2018-09-01 00:00:00

  • Enhancement of ALA-PDT damage by IR-induced hyperthermia on a colon carcinoma model.

    abstract::The interaction of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) and hyperthermia is not well understood. In the present study, significant enhancement of tumor damage was observed after simultaneous application of ALA-PDT and IR-induced hyperthermia using a broad-band incoherent light source. One hour a...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Orenstein A,Kostenich G,Kopolovic Y,Babushkina T,Malik Z

    更新日期:1999-06-01 00:00:00

  • Why is rose bengal more phototoxic to fibroblasts in vitro than in vivo?

    abstract::Photosensitized protein cross-linking has been recently developed to seal wounds and strengthen tissue. Although the photosensitizing dye, Rose Bengal (RB), is phototoxic to cultured cells, cytotoxicity does not accompany RB-photosensitized tissue repair in vivo. We investigated whether the environment surrounding cel...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Yao M,Gu C,Doyle FJ Jr,Zhu H,Redmond RW,Kochevar IE

    更新日期:2014-03-01 00:00:00

  • Are the fluorescent properties of the cyan fluorescent protein sensitive to conditions of oxidative stress?

    abstract::The modifications induced by reactive oxygen species (ROS) on fluorescent proteins (FPs) may have important implications for live cell fluorescence imaging. Using quantitative gamma-radiolysis, we have studied the ROS-induced biochemical and photophysical perturbations on recombinant cyan fluorescent protein (CFP). Af...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Alvarez L,Levin CH,Merola F,Bizouarn T,Pasquier H,Baciou L,Rusconi F,Erard M

    更新日期:2010-01-01 00:00:00

  • DNA damage checkpoint responses in the S phase of synchronized diploid human fibroblasts.

    abstract::We investigated the hypothesis that the strength of the activation of the intra-S DNA damage checkpoint varies within the S phase. Synchronized diploid human fibroblasts were exposed to either 0 or 2.5 J m(-2) UVC in early, mid- and late-S phase. The endpoints measured were the following: (1) radio-resistant DNA synth...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Chastain PD 2nd,Brylawski BP,Zhou YC,Rao S,Chu H,Ibrahim JG,Kaufmann WK,Cordeiro-Stone M

    更新日期:2015-01-01 00:00:00

  • Additional evidence for the cyclic GMP signaling pathway resulting in the photophobic behavior of Stentor coeruleus.

    abstract::We report that exo- and endogenous guanosine 3',5'-cyclic monophosphate (cGMP) specifically influenced the photophobic response. In behavioral experiments the slowly hydrolyzable and membrane-permeable analogs of cGMP (8-bromo-cGMP [Br-cGMP] and N6,2'-o-dibutyryl-cGMP) dramatically prolonged the time for ciliary stop ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Walerczyk M,Fabczak S

    更新日期:2001-12-01 00:00:00

  • Effects of short- and long-term ultraviolet B irradiation on the immune system of the common carp (Cyprinus carpio).

    abstract::Carp (Cyprinus carpio) were repeatedly exposed to 0, 60, 120 and 240 mJ/cm2 ultraviolet B (UVB) radiation three times in 1 week (short-term exposure) or 12 times in 4 weeks (long-term exposure). The effect of UVB on the functioning of the carp immune system was studied on day 2 after the final irradiation. After short...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Markkula SE,Salo HM,Immonen AK,Jokinen EI

    更新日期:2005-05-01 00:00:00

  • Characterization of endogenous protoporphyrin IX induced by delta-aminolevulinic acid in resting and activated peripheral blood lymphocytes by four-color flow cytometry.

    abstract::Lymphocytes treated with delta-aminolevulinic acid (ALA) can accumulate the photoactive, fluorescent heme precursor, protoporphyrin IX (PpIX). With visible light illumination, PpIX can be used in photodynamic therapy (ALA-PDT) to kill or functionally alter cells. The aim of this study was to characterize the effects o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Hryhorenko EA,Rittenhouse-Diakun K,Harvey NS,Morgan J,Stewart CC,Oseroff AR

    更新日期:1998-05-01 00:00:00

  • Spectroscopic studies of cutaneous photosensitizing agents--XIV. The spin trapping of free radicals formed during the photolysis of halogenated salicylanilide antibacterial agents.

    abstract::Several antibacterial halogenated salicylanilides, including 3,3',4',5-tetrachlorosalicylanilide (TCSA) and 3,4',5-tribromosalicylanilide (TBSA) are known to cause photoallergy. We have carried out photochemical and spin trapping studies to determine whether free radicals may be involved in the photoallergic response....

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Chignell CF,Sik RH

    更新日期:1989-09-01 00:00:00

  • Protonation-state-Coupled Conformational Dynamics in Reaction Mechanisms of Channel and Pump Rhodopsins.

    abstract::Channel and pump rhodopsins use energy from light absorbed by a covalently bound retinal chromophore to transport ions across membranes of microbial cells. Ion transfer steps, including proton transfer, can couple to changes in protein conformational dynamics and water positions. Although general principles of how mic...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: Bondar AN,Smith JC

    更新日期:2017-11-01 00:00:00

  • Spectroscopic properties of fluorescein and rhodamine dyes attached to DNA.

    abstract::We report the spectroscopic properties of fluorescein, x-rhodamine, tetramethyl-rhodamine, attached to single strand, duplex DNA, and to the digestion products by DNAse I. The properties reported include: molar absorptivity, quantum yield, absorbance and fluorescence spectra, fluorescence lifetime, intrinsic lifetime ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Delgadillo RF,Parkhurst LJ

    更新日期:2010-03-01 00:00:00

  • Attitudes, Beliefs, and Measures Taken by Parents to Protect Their Children from the Sun in Guangzhou City, China.

    abstract::Excessive sun exposure can cause sunburn, suntan, skin photoaging, and even skin cancer. Skin photoaging conflicts with the human pursuit of a young and beautiful appearance. Some research data indicate that the incidence of skin cancer in the Chinese has been increasing, although it remains lower than in whites. To e...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Wan M,Hu R,Li Y,Wang Y,Xie X,Yue P,Guan L,Lai W

    更新日期:2016-09-01 00:00:00

  • The family of GFP-like proteins: structure, function, photophysics and biosensor applications. Introduction and perspective.

    abstract::In this issue, we offer a symposium-in-print that is focused on several new advancements in fundamental research related to the family of GFP (green fluorescent protein)-like proteins. A few applied aspects are also included to illustrate the impact this amazing set of colored proteins has made on our understanding of...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: Wachter RM

    更新日期:2006-03-01 00:00:00

  • Use of potassium iodide as a chemical actinometer.

    abstract::Aqueous solutions of KI were examined for use as chemical actinometers to measure 254 nm (germicidal) radiation. Irradiation results in electron ejection from iodide such that aqueous electrons and iodine atoms are formed. In the presence of N2O, an electron scavenger, recombination of these two reactive species is el...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Rahn RO

    更新日期:1993-12-01 00:00:00

  • Multispectral imaging autofluorescence microscopy for the analysis of lymph-node tissues.

    abstract::Although histochemical and immunohistochemical methods are the standard procedures in diagnosis of lymphoproliferative disorders, useful improvements in evidencing histopathologic manifestations can be obtained with the introduction of tissue autofluorescence analyses. We used microspectrofluorometry and a Multispectr...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Rigacci L,Alterini R,Bernabei PA,Ferrini PR,Agati G,Fusi F,Monici M

    更新日期:2000-06-01 00:00:00

  • Biological weighting function for the mortality of Boeckella gracilipes (Copepoda, Crustacea) derived from experiments with natural solar radiation.

    abstract::We performed in situ experiments during the austral summer of 1998 to quantify the mortality of the fresh-water copepod Boeckella gracilipes as a function of the UV dose. The copepods were exposed to solar radiation at the water-surface for approximately 24-34 h. Long-pass cut-off filters (Schott) were used in the exp...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Tartarotti B,Cravero W,Zagarese HE

    更新日期:2000-09-01 00:00:00

  • Low-light-induced violaxanthin de-epoxidation in shortly preheated leaves: uncoupling from Delta pH-dependent nonphotochemical quenching.

    abstract::Plants protect themselves against excessive light by the induction of Delta pH-dependent nonphotochemical quenching (qE) that is associated with de-epoxidation of violaxanthin (V) to zeaxanthin (Z) in thylakoid membranes. In this work, we report that low light (12 micromol photons m(-2) s(-1)) is sufficient for a mark...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Ilík P,Kotabová E,Spundová M,Novák O,Kana R,Strzałka K

    更新日期:2010-05-01 00:00:00

  • Optical properties of human melanocytic nevi in vivo.

    abstract::We present an in vivo study of the optical properties of melanin present in melanocytic nevi of human subjects with Fitzpatrick skin type III (Caucasian descent) using optical spectroscopy. We show that the melanin absorption spectrum exhibits an exponential dependence on wavelength with a decay constant which follows...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Zonios G,Dimou A

    更新日期:2009-01-01 00:00:00

  • Supramolecular cationic tetraruthenated porphyrin and light-induced decomposition of 2'-deoxyguanosine predominantly via a singlet oxygen-mediated mechanism.

    abstract::The tetraruthenated porphyrin, mu-[meso-5,10,15,20-tetra(pyridyl)porphyrin]tetrakis[bis-(bipyridine) chloride ruthenium(II)] (TRP) is a supramolecular cationic species. The aim of the present investigation was to evaluate the photodynamic properties of TRP and Zn-TRP to damage DNA with emphasis on the mechanistic aspe...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Ravanat JL,Cadet J,Araki K,Toma HE,Medeiros MH,Mascio PD

    更新日期:1998-11-01 00:00:00

  • Molecular evolution of the Ca(2+)-binding photoproteins of the Hydrozoa.

    abstract::Alignment of the primary structures of the hydrozoan photoproteins, aequorin, mitrocomin, clytin and obelin showed very strong amino acid sequence identities. The Ca(2+)-binding sites of the proteins were found to be highly conserved. The Ca(2+)-binding sites were also homologous to the Ca(2+)-binding sites of other C...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Tsuji FI,Ohmiya Y,Fagan TF,Toh H,Inouye S

    更新日期:1995-10-01 00:00:00