Interaction between human alpha1-acid glycoprotein (orosomucoid) and 2-p-toluidinylnaphthalene-6-sulfonate.

Abstract:

:The interaction between human alpha1-acid glycoprotein (orosomucoid) and the fluorescent probe, 2-p-toluidinylnaphthalene-6-sulfonate (TNS) has been studied. An association constant of 16.7 (+/- 3) x 10(3) M-1 was obtained for the complex at 20 degrees C with a stoichiometry of 1:1. From the effect of temperature on the binding process, the standard enthalpy change for the binding is calculated to be delta H0 = -18 +/- 3 kJ mol-1 and the standard entropy change delta S0 = 19 +/- 12 J K-1 mol-1. The tryptophan fluorescence of the protein can be described by a sum of three exponentials. Upon TNS binding, the average fluorescence lifetime of the protein in the complex changes much less than the fluorescence intensity. The bound TNS is therefore a very efficient acceptor for the protein fluorescence. The TNS bound to orosomucoid present two fluorescence lifetimes 11 and 4.3 ns. The possible origins of the two lifetimes are discussed.

journal_name

Photochem Photobiol

authors

Albani J,Vos R,Willaert K,Engelborghs Y

doi

10.1111/j.1751-1097.1995.tb05234.x

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

30-4

issue

1

eissn

0031-8655

issn

1751-1097

journal_volume

62

pub_type

杂志文章
  • Analysis of compact fluorescent lights for use by patients with photosensitive conditions.

    abstract::Ultraviolet radiation (UVR) is hazardous to patients with photosensitive skin disorders, such as lupus erythematosus, xeroderma pigmentosum and skin cancer. As such, these patients are advised to minimize their exposure to UVR. Classically, this is accomplished through careful avoidance of sun exposure and artificial ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00540.x

    authors: Klein RS,Werth VP,Dowdy JC,Sayre RM

    更新日期:2009-07-01 00:00:00

  • Immunotoxic effects of cis-urocanic acid exposure in C57BL/6N and C3H/HeN mice.

    abstract::Exposure to ultraviolet radiation results in increased levels of intradermal cis-urocanic acid (cUCA) and alters cutaneous immunity by interfering with processing and presentation of antigen by Langerhans cells. Reports on effects of systemic immunotoxicity with 30 day cUCA exposure in laboratory rodents include thymi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2003)077<0383:ieocae>2.0.co;2

    authors: Prater MR,Gogal RM Jr,De Fabo EC,Longstreth J,Holladay SD

    更新日期:2003-04-01 00:00:00

  • Photodynamic therapy with photofrin II induces programmed cell death in carcinoma cell lines.

    abstract::The mode of cell death following photodynamic therapy was investigated from the perspective of programmed cell death or apoptosis. Human prostate carcinoma cells (PC3), human non-small cell lung carcinoma (H322a) and rat mammary carcinoma (MTF7) were treated by photodynamic therapy. An examination of extracted cellula...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1994.tb05066.x

    authors: He XY,Sikes RA,Thomsen S,Chung LW,Jacques SL

    更新日期:1994-04-01 00:00:00

  • Luciferin-Regenerating Enzyme Crystal Structure Is Solved but its Function Is Still Unclear.

    abstract::Contribution of luciferin-regenerating enzyme (LRE) for in vitro recycling of D-luciferin has been reported. According to crystal structure of LRE, it is a beta-propeller protein which is a type of all β-protein architecture. In this overview, reinvestigation of the luciferase-based LRE assays and its function is repo...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12723

    authors: Hosseinkhani S,Emamgholi Zadeh E,Sahebazzamani F,Ataei F,Hemmati R

    更新日期:2017-03-01 00:00:00

  • Interspecific variation in UV defense mechanisms among temperate freshwater fishes.

    abstract::An important step in predicting the effects of future increases in UV radiation (UVR) is to evaluate the mechanisms that organisms use to prevent and repair DNA damage and determine how those mechanisms influence UVR sensitivity. Damage is prevented to varying degrees through photoprotection and repaired via two main ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-08-23-RA-656

    authors: Olson MH,Mitchell DL

    更新日期:2006-03-01 00:00:00

  • The Glucosamine-derivative NAPA Suppresses MAPK Activation and Restores Collagen Deposition in Human Diploid Fibroblasts Challenged with Environmental Levels of UVB.

    abstract::The ultraviolet (UV) component of solar radiation is the driving force of life on earth, but it can cause photoaging and skin cancer. In this study, we investigated the effects of the glucosamine-derivative 2-(N-Acetyl)-L-phenylalanylamido-2-deoxy-β-D-glucose (NAPA) on human primary fibroblasts (FBs) stimulated in vit...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13185

    authors: Lopreiato M,Cocchiola R,Falcucci S,Leopizzi M,Cardone M,Di Maio V,Brocco U,D'Orazi V,Calvieri S,Scandurra R,De Marco F,Scotto d'Abusco A

    更新日期:2020-01-01 00:00:00

  • Stationary headband for clinical time-of-flight optical imaging at the bedside.

    abstract::Conventional brain-imaging modalities may be limited by high cost, difficulty of bedside use, noncontinuous operation, invasiveness or an inability to obtain measurements of tissue function, such as oxygenation during stroke. Our goal was to develop a bedside clinical device able to generate continuous, noninvasive, t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Hintz SR,Benaron DA,van Houten JP,Duckworth JL,Liu FW,Spilman SD,Stevenson DK,Cheong WF

    更新日期:1998-09-01 00:00:00

  • Formation of aggregates of tryptic fragments derived from the carboxyl-terminal half of pea phytochrome and localization of the site of contact between the fragments by amino-terminal amino acid sequence analysis.

    abstract::Limited trypsinization of large pea (Pisum sativum cv. Alaska) phytochrome and subsequent size-exclusion chromatography (SEC) in 0.1 M Na phosphate, pH 7.8, yielded a high-molecular-mass aggregate of tryptic fragments of phytochrome. Further SEC in 0.1 M Tris-HCl, pH 7.5, plus various concentrations of NaSCN, indicate...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1989.tb04136.x

    authors: Yamamoto KT,Tokutomi S

    更新日期:1989-07-01 00:00:00

  • Wavelength dependence of histological, physical, and visible changes in chronically UV-irradiated hairless mouse skin.

    abstract::Albino hairless mice (Skh: HR-1) exposed chronically to sub-erythemal doses of UV radiation display physical, visible and histological alterations. Using narrow bandwidth radiation covering the UV radiation spectrum from 280-380 nm, the wavelength dependence of these alterations was determined. The wavelength dependen...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1989.tb02907.x

    authors: Bissett DL,Hannon DP,Orr TV

    更新日期:1989-12-01 00:00:00

  • Preparation of new magnetic nanocatalysts based on TiO2 and ZnO and their application in improved photocatalytic degradation of dye pollutant under visible light.

    abstract::Photocatalytic degradation of methyl orange (MO) as a model of an organic pollution was accomplished with magnetic and porous TiO(2)/ZnO/Fe(3)O(4)/PANI and ZnO/Fe(3)O(4)/PANI nanocomposites under visible light irradiation. The structures of nanocomposites were characterized by various techniques including UV-Vis absor...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2012.01209.x

    authors: Nabid MR,Sedghi R,Gholami S,Oskooie HA,Heravi MM

    更新日期:2013-01-01 00:00:00

  • Chlorin-bacteriochlorin energy-transfer dyads as prototypes for near-infrared molecular imaging probes: controlling charge-transfer and fluorescence properties in polar media.

    abstract::The photophysical properties of two energy-transfer dyads that are potential candidates for near-infrared (NIR) imaging probes are investigated as a function of solvent polarity. The dyads (FbC-FbB and ZnC-FbB) contain either a free base (Fb) or zinc (Zn) chlorin (C) as the energy donor and a free base bacteriochlorin...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00532.x

    authors: Kee HL,Diers JR,Ptaszek M,Muthiah C,Fan D,Lindsey JS,Bocian DF,Holten D

    更新日期:2009-07-01 00:00:00

  • Assessment of DNA damage induced by broadband and narrowband UVB in cultured lymphoblasts and keratinocytes using the comet assay.

    abstract::Phototherapy with broadband UVB is an effective treatment for inflammatory dermatoses. A newly developed fluorescent UVB lamp (Philips TL01) that emits a narrowband UVB around 311 nm was shown to be superior for the phototherapy of psoriasis. In order to contribute to the knowledge about the carcinogenic potential of ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Tzung TY,Rünger TM

    更新日期:1998-06-01 00:00:00

  • Flavin-based Blue-Light photosensors: a photobiophysics update.

    abstract::This review deals with the biophysical aspects of flavin-based photosensors, comprising cryptochromes, LOV (Light, Oxygen and Voltage) and BLUF (Blue Light sensing Using FAD) proteins. Special emphasis is given to structural issues, photocycle quantum yields and energetics, mechanism of the light-triggered reactions, ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1751-1097.2007.00196.x

    authors: Losi A

    更新日期:2007-11-01 00:00:00

  • Photosensitization with a chlorin-thiobarbiturate conjugate.

    abstract::The photobiologic properties of a chlorin-thiobarbiturate conjugate were examined. In addition to the characteristic 670 nm chlorin absorbance, the conjugate exhibited new absorbance bands at 517 and 743 nm, but these were not a part of the fluorescence emission spectra, nor was fluorescence observed at wavelengths > ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1994.tb02981.x

    authors: Kessel D,Morgan A

    更新日期:1994-05-01 00:00:00

  • Photoreduction of bacteriorhodopsin Schiff base at low humidity. A study with C13=C14 nonisomerizable artificial pigments.

    abstract::The retinal protonated Schiff base of bacteriorhodopsin is photoreactive to reducing agents such as NaBH4. In the present work we have studied the effect of different protein hydration levels on the photoreductive reaction, as well as the consequences of preventing isomerization around the critical C13=C14 retinal dou...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2002)075<0668:pobsba>2.0.co;2

    authors: Aharoni A,Ottolenghi M,Sheves M

    更新日期:2002-06-01 00:00:00

  • Porphyrin-cellulose nanocrystals: a photobactericidal material that exhibits broad spectrum antimicrobial activity.

    abstract::Towards our overall objectives of developing potent antimicrobial materials to combat the escalating threat to human health posed by the transmission of surface-adhering pathogenic bacteria, we have investigated the photobactericidal activity of cellulose nanocrystals that have been modified with a porphyrin-derived p...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2012.01117.x

    authors: Carpenter BL,Feese E,Sadeghifar H,Argyropoulos DS,Ghiladi RA

    更新日期:2012-05-01 00:00:00

  • The origin of magnetic field dependent recombination in alkylcobalamin radical pairs.

    abstract::Magnetic field effect studies of alkylcobalamin photolysis provide evidence for the formation of a reactive radical pair that is born in the singlet spin state. The radical pair recombination process that is responsible for the magnetic field dependence of the continuous-wave (CW) quantum yield is limited to the diffu...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1996.tb02460.x

    authors: Natarajan E,Grissom CB

    更新日期:1996-08-01 00:00:00

  • A multitherapy resistance factor from melanoma reveals that killing by near UV is different from genotoxic agents.

    abstract::A diffusible multitherapy resistance factor (MTRF) is produced by Cloudman S91 melanoma cells in vitro. The MTRF decreases sensitivity of the target cell line, S91/amel, to gamma-irradiation, UVC (200-280 nm) and mitomycin C (MMC). In the present study, we demonstrate that MTRF also increases the survival of S91/amel ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1995.tb02348.x

    authors: Hill HZ,Hill GJ,Cieszka K,Azure M,Chowdhary I,Sayre RM

    更新日期:1995-05-01 00:00:00

  • Advanced glycation endproducts induce photocrosslinking and oxidation of bovine lens proteins through type-I mechanism.

    abstract::Advanced glycation endproducts (AGEs) have been suggested as photosensitizers that are capable of mediating eye lens photo-damage during aging. In the present work, we investigate the photo-crosslinking and oxidation of bovine lens proteins sensitized by AGEs, with special regard to low oxygen conditions. A mechanisti...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00415.x

    authors: Fuentealba D,Friguet B,Silva E

    更新日期:2009-01-01 00:00:00

  • Ultraviolet radiation exposure, vitamin D, and cancer.

    abstract::This paper reviews briefly the evidence for an association between various measures of UV radiation (UVR) exposure and the development of cancer. Issues such as data quality, study design, measurement variation, comparability of studies, and quantification of UV exposure in relation to skin cancer are discussed. A ran...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1562/2005-02-18-IR-445

    authors: Berwick M,Kesler D

    更新日期:2005-11-01 00:00:00

  • Resolution of the individual tryptophanyl contributions to the near-ultraviolet dichroic activity of apomyoglobin.

    abstract::The individual tryptophanyl contributions to the near-ultraviolet dichroic activity of apomyoglobin in its native conformation have been resolved. This was accomplished by comparing the spectra of two classes of apomyoglobin with different aromatic residue contents and observing the effect of a specific modification o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1994.tb09664.x

    authors: Sirangelo I,Irace G,Bismuto E

    更新日期:1994-06-01 00:00:00

  • 8-methoxypsoralen plus UVA induces the 72 kDa heat shock protein in organ-cultured normal human skin.

    abstract::The proteins induced by heat and other stressors, called heat shock proteins (HSP) or stress proteins, are considered to play a general role in protection from cellular injury. Exposure to UVA (320-400 nm) following application of 8-methoxypsoralen (8-MOP), termed PUVA is commonly used in the field of dermatology. In ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1993.tb04974.x

    authors: Muramatsu T,Yamashina Y,Tada H,Kobayashi N,Yamaji M,Ohno H,Shirai T,Takahashi A,Ohnishi T

    更新日期:1993-12-01 00:00:00

  • Deactivation of singlet molecular oxygen by organo-selenium compounds exhibiting glutathione peroxidase activity and by sulfur-containing homologs.

    abstract::The bimolecular rate constants (k) of quenching of molecular singlet oxygen 1O2 (1 delta g) by organo-selenium compounds exhibiting glutathione peroxidase activity and by sulfur analogs have been determined by time resolved phosphorescence detection of 1O2 in CD3OD and C6D6, with no solvent effect. The rate constants ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1991.tb02082.x

    authors: Scurlock R,Rougée M,Bensasson RV,Evers M,Dereu N

    更新日期:1991-11-01 00:00:00

  • Mechanism of radical cation formation from the excited states of zeaxanthin and astaxanthin in chloroform.

    abstract::The C-40 xanthophylls zeaxanthin and astaxanthin were confirmed to form radical cations, Car.+, in the electron-accepting solvent chloroform by direct excitation using subpicosecond time-resolved absorption spectroscopy in combination with spectroelectrochemical determination of the near-infrared absorption of Car.+. ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-09-30-RA-705R.1

    authors: Han RM,Tian YX,Wu YS,Wang P,Ai XC,Zhang JP,Skibsted LH

    更新日期:2006-03-01 00:00:00

  • The influence of ultraviolet-B radiation on growth, hydroxycinnamic acids and flavonoids of Deschampsia antarctica during Springtime ozone depletion in Antarctica.

    abstract::We examined the influence of solar ultraviolet-B radiation (UV-B; 280-320 nm) on the growth, biomass production and phenylpropanoid concentrations of Deschampsia antarctica during the springtime ozone depletion season at Palmer Station, along the Antarctic Peninsula. Treatments involved placing filters on frames over ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2004-09-18-RA-321

    authors: Ruhland CT,Xiong FS,Clark WD,Day TA

    更新日期:2005-09-01 00:00:00

  • Photosensitization with bacteriochlorins.

    abstract::Biophysical and photobiological properties of a group of bacteriochlorins were compared with efficacy of these products for photodynamic therapy of murine tumors. Predictive factors for selective photosensitization in vivo include affinity binding to lipoproteins greater than albumin, extinction coefficient at the wav...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1993.tb09549.x

    authors: Kessel D,Smith KM,Pandey RK,Shiau FY,Henderson B

    更新日期:1993-08-01 00:00:00

  • Behaviors producing photodispersal in Stentor coeruleus.

    abstract::The ciliate Stentor coeruleus exhibits photodispersal, that is, these cells swim away from light sources and collect in dimly lighted areas. We imaged and reconstructed the tracks of 48 Stentor to determine which swimming behaviors produced their photodispersal. We observed that their photodispersal is not due to a ch...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2004)080<0401:BPPISC>2.0.CO;2

    authors: Menzies E,Das N,Wood DC

    更新日期:2004-11-01 00:00:00

  • The role of urocanic acid in UV-induced immunosuppression: recent advances (1992-1994).

    abstract::Cis-urocanic acid (UCA), formed in the epidermis by UV irradiation of trans-UCA, has been implicated as a mediator of the immunosuppression induced by UV exposure of the skin. This review covers recent work in which the wavelength dependence of cis-UCA formation, the interaction of UCA isomers with DNA, the effects of...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1751-1097.1995.tb05261.x

    authors: Norval M,Gibbs NK,Gilmour J

    更新日期:1995-08-01 00:00:00

  • Low-light-induced violaxanthin de-epoxidation in shortly preheated leaves: uncoupling from Delta pH-dependent nonphotochemical quenching.

    abstract::Plants protect themselves against excessive light by the induction of Delta pH-dependent nonphotochemical quenching (qE) that is associated with de-epoxidation of violaxanthin (V) to zeaxanthin (Z) in thylakoid membranes. In this work, we report that low light (12 micromol photons m(-2) s(-1)) is sufficient for a mark...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00699.x

    authors: Ilík P,Kotabová E,Spundová M,Novák O,Kana R,Strzałka K

    更新日期:2010-05-01 00:00:00

  • Applicability of the polysulphone horizontal calibration to differently inclined dosimeters.

    abstract::Polysulphone (PS) dosimetry has been a widely used technique for more than 30 years to quantify the erythemally effective UV dose received by anatomic sites (personal exposure). The calibration of PS dosimeters is an important issue as their spectral response is different from the erythemal action spectrum. It is perf...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2011.01006.x

    authors: Casale GR,Siani AM,Diémoz H,Kimlin MG,Colosimo A

    更新日期:2012-01-01 00:00:00