Abstract:
:The interaction between human alpha1-acid glycoprotein (orosomucoid) and the fluorescent probe, 2-p-toluidinylnaphthalene-6-sulfonate (TNS) has been studied. An association constant of 16.7 (+/- 3) x 10(3) M-1 was obtained for the complex at 20 degrees C with a stoichiometry of 1:1. From the effect of temperature on the binding process, the standard enthalpy change for the binding is calculated to be delta H0 = -18 +/- 3 kJ mol-1 and the standard entropy change delta S0 = 19 +/- 12 J K-1 mol-1. The tryptophan fluorescence of the protein can be described by a sum of three exponentials. Upon TNS binding, the average fluorescence lifetime of the protein in the complex changes much less than the fluorescence intensity. The bound TNS is therefore a very efficient acceptor for the protein fluorescence. The TNS bound to orosomucoid present two fluorescence lifetimes 11 and 4.3 ns. The possible origins of the two lifetimes are discussed.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Albani J,Vos R,Willaert K,Engelborghs Ydoi
10.1111/j.1751-1097.1995.tb05234.xsubject
Has Abstractpub_date
1995-07-01 00:00:00pages
30-4issue
1eissn
0031-8655issn
1751-1097journal_volume
62pub_type
杂志文章abstract::Dark-grown leaves of maize (Zea mays), wheat (Triticum aestivum), wild-type pea (Pisum sativum) and its light-independent photomorphogenesis mutant (lip1) have different proportions of protochlorophyllide (Pchlide) forms as revealed by low-temperature fluorescence emission spectra. Four discrete spectral forms of Pchl...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2003)078<0205:flopip>2.0.co;2
更新日期:2003-08-01 00:00:00
abstract::Catalases are oxidized by singlet oxygen giving rise to more acidic conformers detected in zymograms after electrophoresis in polyacrylamide gels. This shift in catalase mobility can be indicative of singlet oxygen production in vivo. Catalase from human cells, as from many organisms, is susceptible to in vitro modifi...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Vitamin D production in human skin occurs only when incident UV radiation exceeds a certain threshold. From simulations of UV irradiances worldwide and throughout the year, we have studied the dependency of the extent and duration of cutaneous vitamin D production in terms of latitude, time, total ozone, clouds, aeros...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-11-19-RN-375
更新日期:2005-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb08624.x
更新日期:1995-04-01 00:00:00
abstract::A solution of iodouracil (0.2 mg/mL) and KI (0.1 M) in 0.23 M borate buffer (pH 7) forms triiodide upon exposure to UVB. In the presence of thyodene, the blue starch-iodine complex is formed, the intensity of which is proportional to the amount of UVB exposure. Studies were conducted using either a sunlamp or solar ir...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::The phototoxic anti-hyperlipoproteinemic drug fenofibrate was found to be photolabile under aerobic and anaerobic conditions. Irradiation under argon of a methanol solution of this drug produced the photoproducts isopropyl 4-(1-[4-chlorophenyl]-1,2-dihydroxy)ethylphenoxyisobutyrate, 1,2-bis(4-chlorophenyl)-1,2bis (4-[...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb04917.x
更新日期:1993-10-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00727.x
更新日期:2010-07-01 00:00:00
abstract::Macroalgae distributed in intertidal zones experience a series of environmental changes, such as periodical desiccation associated with tidal cycles, increasing CO2 concentration and solar UVB (280-315 nm) irradiance in the context of climate change. We investigated how the economic red macroalga, Pyropia haitanensis,...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12531
更新日期:2015-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)072<0714:wacgei>2.0.co;2
更新日期:2000-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)074<0453:copaqt>2.0.co;2
更新日期:2001-09-01 00:00:00
abstract::Herein, we present a comparative study about the photoinactivation of Staphylococcus aureus (Gram-positive model) and Escherichia coli (Gram-negative model) employing a neutral and a dicationic axially functionalized SiIV -phthalocyanine. Depending on the charge of the siloxane moiety (neutral monosaccharide or cation...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12881
更新日期:2018-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12215
更新日期:2014-03-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00617.x
更新日期:2010-01-01 00:00:00
abstract::We investigated the hypothesis that the strength of the activation of the intra-S DNA damage checkpoint varies within the S phase. Synchronized diploid human fibroblasts were exposed to either 0 or 2.5 J m(-2) UVC in early, mid- and late-S phase. The endpoints measured were the following: (1) radio-resistant DNA synth...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12361
更新日期:2015-01-01 00:00:00
abstract::We report that exo- and endogenous guanosine 3',5'-cyclic monophosphate (cGMP) specifically influenced the photophobic response. In behavioral experiments the slowly hydrolyzable and membrane-permeable analogs of cGMP (8-bromo-cGMP [Br-cGMP] and N6,2'-o-dibutyryl-cGMP) dramatically prolonged the time for ciliary stop ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)074<0829:aeftcg>2.0.co;2
更新日期:2001-12-01 00:00:00
abstract::Carp (Cyprinus carpio) were repeatedly exposed to 0, 60, 120 and 240 mJ/cm2 ultraviolet B (UVB) radiation three times in 1 week (short-term exposure) or 12 times in 4 weeks (long-term exposure). The effect of UVB on the functioning of the carp immune system was studied on day 2 after the final irradiation. After short...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-07-13-RA-231
更新日期:2005-05-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1989.tb04162.x
更新日期:1989-09-01 00:00:00
abstract::Channel and pump rhodopsins use energy from light absorbed by a covalently bound retinal chromophore to transport ions across membranes of microbial cells. Ion transfer steps, including proton transfer, can couple to changes in protein conformational dynamics and water positions. Although general principles of how mic...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.12790
更新日期:2017-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00663.x
更新日期:2010-03-01 00:00:00
abstract::Excessive sun exposure can cause sunburn, suntan, skin photoaging, and even skin cancer. Skin photoaging conflicts with the human pursuit of a young and beautiful appearance. Some research data indicate that the incidence of skin cancer in the Chinese has been increasing, although it remains lower than in whites. To e...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12623
更新日期:2016-09-01 00:00:00
abstract::In this issue, we offer a symposium-in-print that is focused on several new advancements in fundamental research related to the family of GFP (green fluorescent protein)-like proteins. A few applied aspects are also included to illustrate the impact this amazing set of colored proteins has made on our understanding of...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1562/2005-10-02-IR-708
更新日期:2006-03-01 00:00:00
abstract::Aqueous solutions of KI were examined for use as chemical actinometers to measure 254 nm (germicidal) radiation. Irradiation results in electron ejection from iodide such that aqueous electrons and iodine atoms are formed. In the presence of N2O, an electron scavenger, recombination of these two reactive species is el...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb04986.x
更新日期:1993-12-01 00:00:00
abstract::Although histochemical and immunohistochemical methods are the standard procedures in diagnosis of lymphoproliferative disorders, useful improvements in evidencing histopathologic manifestations can be obtained with the introduction of tissue autofluorescence analyses. We used microspectrofluorometry and a Multispectr...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)071<0737:miamft>2.0.co;2
更新日期:2000-06-01 00:00:00
abstract::We performed in situ experiments during the austral summer of 1998 to quantify the mortality of the fresh-water copepod Boeckella gracilipes as a function of the UV dose. The copepods were exposed to solar radiation at the water-surface for approximately 24-34 h. Long-pass cut-off filters (Schott) were used in the exp...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)072<0314:bwfftm>2.0.co;2
更新日期:2000-09-01 00:00:00
abstract::Plants protect themselves against excessive light by the induction of Delta pH-dependent nonphotochemical quenching (qE) that is associated with de-epoxidation of violaxanthin (V) to zeaxanthin (Z) in thylakoid membranes. In this work, we report that low light (12 micromol photons m(-2) s(-1)) is sufficient for a mark...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00699.x
更新日期:2010-05-01 00:00:00
abstract::We present an in vivo study of the optical properties of melanin present in melanocytic nevi of human subjects with Fitzpatrick skin type III (Caucasian descent) using optical spectroscopy. We show that the melanin absorption spectrum exhibits an exponential dependence on wavelength with a decay constant which follows...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00436.x
更新日期:2009-01-01 00:00:00
abstract::The tetraruthenated porphyrin, mu-[meso-5,10,15,20-tetra(pyridyl)porphyrin]tetrakis[bis-(bipyridine) chloride ruthenium(II)] (TRP) is a supramolecular cationic species. The aim of the present investigation was to evaluate the photodynamic properties of TRP and Zn-TRP to damage DNA with emphasis on the mechanistic aspe...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Alignment of the primary structures of the hydrozoan photoproteins, aequorin, mitrocomin, clytin and obelin showed very strong amino acid sequence identities. The Ca(2+)-binding sites of the proteins were found to be highly conserved. The Ca(2+)-binding sites were also homologous to the Ca(2+)-binding sites of other C...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb08713.x
更新日期:1995-10-01 00:00:00