Abstract:
:Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in comparison to that of FCS-cultured JPCs. By cell impedance measurements, we detected significantly higher population doubling times of PL-cultured JPCs in comparison to FCS-cultured JPCs. However, this result was not based on lower proliferation activities but on diminished cell sizes which JPCs develop under PL cultivation. The measurements of the metabolic activities clearly showed significantly higher cell proliferation rates under PL culturing. Equivalent levels of the mesenchymal cell markers CD29, CD45, CD73, CD90, and CD105 were detected, but there were significantly increased MSCA-1 levels under PL cultivation. While JPCs only occasionally mineralize under FCS culture conditions, the mineralization potential was significantly stronger under PL cultivation. Moreover, in 4 of 5 analyzed patient cells, the addition of dexamethasone was proved no longer necessary for strong mineralization of PL-cultured JPCs. We conclude that in vitro cultivation of JPCs with platelet lysate is a suitable alternative to FCS culture conditions and a powerful tool for the development of high-quality TE constructs using jaw periosteal cells.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Wanner Y,Umrath F,Waidmann M,Reinert S,Alexander Ddoi
10.1155/2017/8303959subject
Has Abstractpub_date
2017-01-01 00:00:00pages
8303959eissn
1687-966Xissn
1687-9678journal_volume
2017pub_type
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journal_title:Stem cells international
pub_type: 杂志文章
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journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章
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journal_title:Stem cells international
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pub_type: 杂志文章,评审
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journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.4061/2011/310928
更新日期:2011-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/6439864
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/8216312
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/921053
更新日期:2012-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/7813729
更新日期:2018-04-03 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
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journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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更新日期:2015-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3134543
更新日期:2017-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/376918
更新日期:2014-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2015/148064
更新日期:2015-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/7943495
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/7267142
更新日期:2019-12-27 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/796215
更新日期:2015-01-01 00:00:00