Abstract:
:There are very few model systems which demonstrate hematopoiesis in vitro. Previously, we described unique splenic stromal cell lines which support the in vitro development of hematopoietic cells and particularly myeloid cells. Here, the 5G3 spleen stromal cell line has been investigated for capacity to support the differentiation of hematopoietic cells from progenitors in vitro. Initially, 5G3 was shown to express markers of mesenchymal but not endothelial or hematopoietic cells and to resemble perivascular reticular cells in the bone marrow through gene expression. In particular, 5G3 resembles CXCL12-abundant reticular cells or perivascular reticular cells, which are important niche elements for hematopoiesis in the bone marrow. To analyse the hematopoietic support function of 5G3, specific signaling pathway inhibitors were tested for the ability to regulate cell production in vitro in cocultures of stroma overlaid with bone marrow-derived hematopoietic stem/progenitor cells. These studies identified an important role for Wnt and Notch pathways as well as tyrosine kinase receptors like c-KIT and PDGFR. Cell production in stromal cocultures constitutes hematopoiesis, since signaling pathways provided by splenic stroma reflect those which support hematopoiesis in the bone marrow.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Lim HK,Periasamy P,O'Neill HCdoi
10.1155/2018/9896142subject
Has Abstractpub_date
2018-12-25 00:00:00pages
9896142eissn
1687-966Xissn
1687-9678journal_volume
2018pub_type
杂志文章abstract::This study is aimed at investigating whether human umbilical cord mesenchymal stem cell- (hucMSC-) derived exosomes (hucMSC-exosomes) have a protective effect on acute myocardial infarction (AMI). Exosomes were characterized under transmission electron microscopy and the particles of exosomes were further examined thr...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/761643
更新日期:2015-01-01 00:00:00
abstract::Periodontal ligament stem cells (PDLSCs) possess self-renewal, multilineage differentiation, and immunomodulatory properties. They play a crucial role in maintaining periodontal homeostasis and also participated in orthodontic tooth movement (OTM). Various studies have applied controlled mechanical stimulation to PDLS...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/6531216
更新日期:2018-09-17 00:00:00
abstract::A growing body of evidence supports the argument that bone marrow-derived mesenchymal stem cells (MSCs) can differentiate into cardiomyocyte-like cells in an appropriate cellular environment, but the differentiation rate is low. A cocktail method was designed: we investigated the role of 5-azacytidine (5-aza), salvian...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/162024
更新日期:2014-01-01 00:00:00
abstract::Within the field of regenerative medicine, many have sought to use stem cells as a promising way to heal human tissue; however, in the past few years, exosomes (packaged vesicles released from cells) have shown more exciting promise. Specifically, stem cell-derived exosomes have demonstrated great ability to provide t...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/5802529
更新日期:2016-01-01 00:00:00
abstract::Whether liver epithelial cells contribute to the development of hepatic scarring by undergoing epithelial-to-mesenchymal transition (EMT) is a controversial issue. Herein, we revisit the concept of EMT in cholangiopathies, a group of severe hepatic disorders primarily targeting the bile duct epithelial cell (cholangio...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/2953727
更新日期:2016-01-01 00:00:00
abstract::Transcription factors are tools repetitively used by the embryo to generate a variety of lineages. Hence, their context of activation is an important determinant of their ability to specifically trigger certain cell fates, but not others. The context is also consequential when considering directing differentiation of ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/6939438
更新日期:2016-01-01 00:00:00
abstract::The migration of administered mesenchymal stem cells (MSCs) to sites of injury via the bloodstream has been demonstrated. However, the underlying mechanisms of umbilical cord MSC adhesion to endothelial cells during transendothelial migration are still unclear. In this study, our data showed that IL-1β induced LFA-1 e...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/7267142
更新日期:2019-12-27 00:00:00
abstract::Aging, which is accompanied by decreased organ function and increased disease incidence, limits human lifespan and has attracted investigators for thousands of years. In recent decades, with the rapid development of biology, scientists have shown that epigenetic modifications, especially DNA methylation, are key regul...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/1047896
更新日期:2020-07-08 00:00:00
abstract::[This corrects the article DOI: 10.1155/2017/3978595.]. ...
journal_title:Stem cells international
pub_type: 已发布勘误
doi:10.1155/2017/9438310
更新日期:2017-01-01 00:00:00
abstract::Known problems of the autologous chondrocyte implantation motivate the search for cellular alternatives. The aim of the study was to test the potential of synovium-derived stem cells (SMSC) to regenerate cartilage using a matrix-associated implantation. In an osteochondral defect model of the medial femoral condyle in...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/4142031
更新日期:2018-04-19 00:00:00
abstract::Cardiac progenitor cells (CPCs) have rapidly advanced to clinical trials, yet little is known regarding their interaction with the microenvironment. Signaling cues present in the microenvironment change with development and disease. This work aims to assess the influence of two distinct signaling moieties on CPCs: cyc...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/8364382
更新日期:2016-01-01 00:00:00
abstract::Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WD...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/7848932
更新日期:2017-01-01 00:00:00
abstract:Background:Mesenchymal stem cell therapy (MSCT) and defocused low-energy shock wave therapy (ESWT) has been shown to ameliorate erectile dysfunction (ED). However, the interactions and effects of action between MSCT and ESWT remain poorly understood. In this study, we investigated the mechanisms of combination therapy ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/1302672
更新日期:2018-08-29 00:00:00
abstract::Human mesenchymal stem/stromal cells (hMSCs) have generated great interest in regenerative medicine mainly due to their multidifferentiation potential and immunomodulatory role. Although hMSC can be obtained from different tissues, the number of available cells is always low for clinical applications, thus requiring i...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/6597815
更新日期:2017-01-01 00:00:00
abstract:Materials and Methods:Ischemic brain injury was induced by dMCAO in Sprague-Dawley rats. The transplantation group received MSC infusion 1 h after dMCAO. Expression of IGF-1 in GFAP+ astrocytes, Iba-1+ microglia/macrophages, CD3+ lymphocytes, Ly6C+ monocytes/macrophages, and neutrophil elastase (NE)+ neutrophils was ex...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8853444
更新日期:2020-09-01 00:00:00
abstract::Hepatotoxicity is one of the most cited reasons for withdrawal of approved drugs from the market. The use of nonclinically relevant in vitro and in vivo testing systems contributes to the high attrition rates. Recent advances in differentiating human induced pluripotent stem cells (hiPSCs) into pure cultures of hepato...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/2475631
更新日期:2016-01-01 00:00:00
abstract::Background. The aim of the study was to assess the histological effects of autologous infusion of adipose-derived stem cells (ADSC) on a chronic vocal fold scar in a rabbit model as compared to an untreated scar as well as in injection of hyaluronic acid. Study Design. Animal experiment. Method. We used 74 New Zealand...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/9010279
更新日期:2016-01-01 00:00:00
abstract::The lung is a radiosensitive organ, which imposes limits on the therapeutic dose in thoracic radiotherapy. Irradiated alveolar epithelial cells promote radiation-related pneumonitis and fibrosis. However, the role of lung stem cells (LSCs) in the development of radiation-induced lung injury is still unclear. In this s...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/6312053
更新日期:2020-09-29 00:00:00
abstract::The cornea is a clear structure, void of blood, and lymphatic vessels, functioning as our window to the world. Limbal epithelial stem cells, occupying the area between avascular cornea and vascularized conjunctiva, have been implicated in tissue border maintenance, preventing conjunctivalisation and propagation of blo...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/8620172
更新日期:2018-05-08 00:00:00
abstract:Background:Human dermal fibroblasts (HDFs) are the primary cells in skin and are associated with UVB-induced skin photoaging. Adipose-derived stem cells (ASCs) have been proposed as a treatment for skin aging. The goal of this study was to investigate paracrine mechanisms by which ASCs repair HDFs damage from UVB expos...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8878370
更新日期:2020-12-17 00:00:00
abstract::Titanium (Ti) is widely used as a biomaterial for endosseous implants due to its relatively inert surface oxide layer that enables implanted devices the ability of assembling tissue reparative components that culminate in osseointegration. Topographic modifications in the form of micro- and nanoscaled structures signi...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/5214501
更新日期:2019-07-01 00:00:00
abstract::Stem cell has immense potential in regenerative cellular therapy. Mesenchymal stem cells (MSCs) can become a potential attractive candidate for therapy due to its remarkable ability of self-renewal and differentiation into three lineages, i.e., ectoderm, mesoderm, and endoderm. Stem cell holds tremendous promises in t...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2019/7513404
更新日期:2019-07-24 00:00:00
abstract:Background:To reduce morbidity to cleft patients, new approaches have been developed and here, we report for the first time the use of deciduous dental pulp stem cells (DDPSC) associated with a hydroxyapatite-collagen sponge (Bio-Oss Collagen® 250 mg, Geistlich) for closing alveolar defects during secondary dental erup...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/6234167
更新日期:2020-03-12 00:00:00
abstract::Glucocorticoid-induced osteonecrosis of the femoral head (ONFH) is a hip disorder, and it threatens patients who require megadose of steroid therapies. Nowadays, no valid therapies can reverse the development of GC-induced ONFH once it occurs. Stem cell therapy to GC-induced ONFH would be a promising choice. Although ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/1340252
更新日期:2018-05-22 00:00:00
abstract::Neuromuscular diseases are caused by functional defects of skeletal muscles, directly via muscle pathology or indirectly via disruption of the nervous system. Extensive studies have been performed to improve the outcomes of therapies; however, effective treatment strategies have not been fully established for any majo...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/6241681
更新日期:2018-04-11 00:00:00
abstract::Due to their immunomodulatory and chemotactic properties, hMSC are being explored to treat immune-related diseases. For their use in human therapies, it is necessary to culture hMSC in xeno-free conditions. In this study, the impact that a xeno-free medium based on a human plasma derivate has on these properties was a...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/2185351
更新日期:2017-01-01 00:00:00
abstract::Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effec...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/5654917
更新日期:2018-03-19 00:00:00
abstract::Coronary revascularization remains the standard treatment for obstructive coronary artery disease and can be accomplished by either percutaneous coronary intervention (PCI) or coronary artery bypass graft surgery. Considerable advances have rendered PCI the most common form of revascularization and improved clinical o...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/8270498
更新日期:2017-01-01 00:00:00
abstract::Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, characterized by progressive cartilage degradation, subchondral bone remodeling, synovitis, and chronic pain. Due to the limited self-healing capacity in condylar cartilage, traditional clinical treatments have limited symptom-modifying and str...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/5979741
更新日期:2017-01-01 00:00:00
abstract::MicroRNAs (miRNAs) play important roles in cell fate decisions. However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored. Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mES...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/1769298
更新日期:2017-01-01 00:00:00