Abstract：
:Cardiac progenitor cells (CPCs) have rapidly advanced to clinical trials, yet little is known regarding their interaction with the microenvironment. Signaling cues present in the microenvironment change with development and disease. This work aims to assess the influence of two distinct signaling moieties on CPCs: cyclic biaxial strain and extracellular matrix. We evaluate four endpoints for improving CPC therapy: paracrine signaling, proliferation, connexin43 expression, and alignment. Vascular endothelial growth factor A (about 900 pg/mL) was secreted by CPCs cultured on fibronectin and collagen I. The application of mechanical strain increased vascular endothelial growth factor A secretion 2-4-fold for CPCs cultured on poly-L-lysine, laminin, or a naturally derived cardiac extracellular matrix. CPC proliferation was at least 25% higher on fibronectin than that on other matrices, especially for lower strain magnitudes. At 5% strain, connexin43 expression was highest on fibronectin. With increasing strain magnitude, connexin43 expression decreased by as much as 60% in CPCs cultured on collagen I and a naturally derived cardiac extracellular matrix. Cyclic mechanical strain induced the strongest CPC alignment when cultured on fibronectin or collagen I. This study demonstrates that culturing CPCs on fibronectin with 5% strain magnitude is optimal for their vascular endothelial growth factor A secretion, proliferation, connexin43 expression, and alignment.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
French KM,Maxwell JT,Bhutani S,Ghosh-Choudhary S,Fierro MJ,Johnson TD,Christman KL,Taylor WR,Davis MEdoi
10.1155/2016/8364382subject
Has Abstractpub_date
2016-01-01 00:00:00pages
8364382eissn
1687-966Xissn
1687-9678journal_volume
2016pub_type
杂志文章abstract：:Background. The aim of the study was to assess the histological effects of autologous infusion of adipose-derived stem cells (ADSC) on a chronic vocal fold scar in a rabbit model as compared to an untreated scar as well as in injection of hyaluronic acid. Study Design. Animal experiment. Method. We used 74 New Zealand...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/9010279
更新日期：2016-01-01 00:00:00
abstract：:Adipogenesis is the process through which preadipocytes differentiate into adipocytes. During this process, the preadipocytes cease to proliferate, begin to accumulate lipid droplets, and develop morphologic and biochemical characteristics of mature adipocytes. Mesenchymal stem cells (MSCs) are a type of adult stem ce...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2020/8892300
更新日期：2020-08-12 00:00:00
abstract：:Wnt signalling has been implicated in the regulation of stem cell self-renewal and differentiation; however, the majority of in vitro studies are carried out using monolayer 2D culture techniques. Here, we used mesenchymal stromal cell (MSC) EGFP reporter lines responsive to Wnt pathway activation in a 3D spheroid cul...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/7132529
更新日期：2016-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are currently defined as multipotent stromal cells that undergo sustained in vitro growth and can give rise to cells of multiple mesenchymal lineages, such as adipocytes, chondrocytes, and osteoblasts. The regenerative and immunosuppressive properties of MSCs have led to numerous clinical...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2013/507301
更新日期：2013-01-01 00:00:00
abstract：:Asherman's Syndrome (AS) is an uncommon, acquired, and refractory gynecological disorder. Current treatment was still limited, and stem cell-based therapy has been proposed as a novel strategy for management of AS. Here, we conducted a meta-analysis of self-controlled clinical trials to assess the effectiveness and sa...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/8820538
更新日期：2020-12-19 00:00:00
abstract：:As mesenchymal stem cells (MSCs) are being investigated for regenerative therapies to be used in the clinic, delineating the roles of the IGF system in MSC growth and differentiation, in vitro, is vital in developing these cellular therapies to treat degenerative diseases. Muscle differentiation is a multistep process...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/9245938
更新日期：2019-02-17 00:00:00
abstract：:Female infertility impacts the quality of life and well-being of affected individuals and couples. Female reproductive diseases, such as primary ovarian insufficiency, polycystic ovary syndrome, endometriosis, fallopian tube obstruction, and Asherman syndrome, can induce infertility. In recent years, translational med...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2019/9071720
更新日期：2019-12-06 00:00:00
abstract：:Bone marrow stromal cells (BMSCs) may differentiate into nerve cells under a certain condition; however, the clinical application for treating nervous system disease remains unclear. The aim is to assess the safety profile, feasibility, and effectiveness of surgery combined with autologous BMSCs transplantation for tr...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2015/318269
更新日期：2015-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are multipotent stem cells characterized by self-renewal, production of clonal cell populations, and multilineage differentiation. They exist in nearly all tissues and play a significant role in tissue repair and regeneration. Additionally, MSCs possess wide immunoregulatory properties vi...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2018/3057624
更新日期：2018-06-14 00:00:00
abstract：:Whether liver epithelial cells contribute to the development of hepatic scarring by undergoing epithelial-to-mesenchymal transition (EMT) is a controversial issue. Herein, we revisit the concept of EMT in cholangiopathies, a group of severe hepatic disorders primarily targeting the bile duct epithelial cell (cholangio...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/2953727
更新日期：2016-01-01 00:00:00
abstract：:Due to their immunomodulatory and chemotactic properties, hMSC are being explored to treat immune-related diseases. For their use in human therapies, it is necessary to culture hMSC in xeno-free conditions. In this study, the impact that a xeno-free medium based on a human plasma derivate has on these properties was a...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2017/2185351
更新日期：2017-01-01 00:00:00
abstract：:Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are characterized by their unique capacity to stepwise differentiate towards any particular cell type in an adult organism. Pluripotent stem cells provide a beneficial platform to model hereditary diseases and even cancer development. While the inc...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2019/9301382
更新日期：2019-02-25 00:00:00
abstract：Background:While regenerative stem cell therapy for ischemic heart disease has moved into phase 3 studies, little is still known about retention and migration of cell posttransplantation. In human studies, the ability to track transplanted cells has been limited to labeling with radioisotopes and tracking using nuclear...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/2754927
更新日期：2019-11-14 00:00:00
abstract：:In cholestatic liver diseases, impaired bile excretion disrupts lipid homeostasis. We investigated changes of lipid metabolism, including mitochondrial β-oxidation, in a rat model of bile duct ligation (BDL) in which chorionic plate-derived mesenchymal stem cells (CP-MSCs) were transplanted. Serum cholesterol level, w...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2017/5180579
更新日期：2017-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) possess immunosuppressive properties and have been described in the tumor microenvironment of glioblastoma multiforme (GBM). This manuscript has two major topics-first, to describe isolated and cultured MSCs derived from GBM (GB-MSCs) and second, to examine their in vitro immunosuppressiv...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/6904638
更新日期：2019-05-02 00:00:00
abstract：:Tumor hypoxia is associated with malignant biological phenotype including enhanced angiogenesis and metastasis. Hypoxia increases the expression of vascular endothelial cell growth factor (VEGF), which directly participates in angiogenesis by recruiting endothelial cells into hypoxic area and stimulating their prolife...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/6439864
更新日期：2016-01-01 00:00:00
abstract：:Allogeneic cell-based therapies using adipose tissue-derived stromal cells (ASCs) offer an off-the-shelf alternative to autologous therapy. An underlying assumption is that ASC can modulate the immune response of the recipient. However, in vitro models are required to explore and identify cell interactions and mechani...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/8868909
更新日期：2020-10-26 00:00:00
abstract：:Transcription factors are tools repetitively used by the embryo to generate a variety of lineages. Hence, their context of activation is an important determinant of their ability to specifically trigger certain cell fates, but not others. The context is also consequential when considering directing differentiation of ...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/6939438
更新日期：2016-01-01 00:00:00
abstract：:Substantial progress has been made in identifying the extracellular signalling pathways that regulate neural stem and precursor cell biology in the central nervous system (CNS). The bone morphogenetic proteins (BMPs), in particular BMP4, are key players regulating neuronal and glial cell development from neural precur...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/9260592
更新日期：2016-01-01 00:00:00
abstract：:Human mesenchymal stem/stromal cells (hMSCs) have generated great interest in regenerative medicine mainly due to their multidifferentiation potential and immunomodulatory role. Although hMSC can be obtained from different tissues, the number of available cells is always low for clinical applications, thus requiring i...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2017/6597815
更新日期：2017-01-01 00:00:00
abstract：:During embryonic development pluripotency is progressively lost irreversibly by cell division, differentiation, migration and organ formation. Terminally differentiated cells do not generate other kinds of cells. Pluripotent stem cells are a great source of varying cell types that are used for tissue regeneration or r...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.4061/2011/619583
更新日期：2011-01-01 00:00:00
abstract：:The purpose of this study is to explore why type 2 diabetes mellitus (T2DM) patients are susceptible to pulmonary tuberculosis through detection of serum Toll-like receptor 4 (TLR4), an important immune-related receptor, especially in terms of content and TLR4 gene polymorphism. Patients with T2DM complicated by pulmo...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/8160318
更新日期：2016-01-01 00:00:00
abstract：Objective:Mesenchymal stem cells (MSCs) are considered a promising therapy for wound healing. Here, we explored the role of c-Jun in diabetic wound healing using human umbilical cord-derived MSCs (hUC-MSCs). Methods:Freshly isolated hUC-MSCs were subjected to extensive in vitro subcultivation. The cell proliferative a...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/7430968
更新日期：2020-01-14 00:00:00
abstract：:Tendon healing is fraught with complications such as reruptures and adhesion formation due to the formation of scar tissue at the injury site as opposed to the regeneration of native tissue. Stem cells are an attractive option in developing cell-based therapies to improve tendon healing. However, several questions rem...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2012/291431
更新日期：2012-01-01 00:00:00
abstract：:Podocyte death and regeneration are major topics in kidney research but remain controversial. Data obtained in humans demonstrate the existence of cells sited along Bowman's capsule that behave as podocyte progenitors in vitro and in in vivo mouse models of podocyte injury xenotrasplanted with this human-derived popul...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2015/678347
更新日期：2015-01-01 00:00:00
abstract：:The Wnt signalling pathway plays a crucial role in the development of the nervous system. This signalling cascade is initiated upon binding of the secreted Wnt ligand to a member of the family of frizzled receptors. In the present study, we analysed the presence of frizzled-4 in the enteric nervous system of human inf...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/9076823
更新日期：2016-01-01 00:00:00
abstract：:Faced with the end stage of heart disease, the current treatments only slow worsening of heart failure. Stem cells have the potential of self-renewal and differentiation. It is expected to replace and repair damaged myocardium. But many clinical trials have shown that the stem cell therapy of heart failure is modest o...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.4061/2011/310928
更新日期：2011-01-01 00:00:00
abstract：:Two of the main problems of stem cell and regenerative medicine are the exit of pluripotency and differentiation to functional cells or tissues. The answer to these two problems holds great value in the clinical translation of stem cell as well as regenerative medicine research. Although piling researches have reveale...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/8483035
更新日期：2020-02-03 00:00:00
abstract：:Fifty-six aphaereses were performed in 23 pediatric patients with malignant hematological and solid tumors, following three different protocols for PBPC mobilization and distributed as follows: A: seventeen mobilized with 4 g/m(2) of cyclophosphamide (CFA) and 10 μg/kg/day of granulocyte colony stimulating factor (G-C...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/4078215
更新日期：2016-01-01 00:00:00
abstract：:Here, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells. While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like cells can be isolated...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/8216312
更新日期：2016-01-01 00:00:00
