Abstract:
:Mesenchymal stem cells (MSCs) are currently defined as multipotent stromal cells that undergo sustained in vitro growth and can give rise to cells of multiple mesenchymal lineages, such as adipocytes, chondrocytes, and osteoblasts. The regenerative and immunosuppressive properties of MSCs have led to numerous clinical trials exploring their utility for the treatment of a variety of diseases (e.g., acute graft-versus-host disease, Crohn's disease, multiple sclerosis, osteoarthritis, and cardiovascular diseases including heart failure and myocardial infarction). On the other hand, conventionally cultured MSCs reflect heterogeneous populations that often contain contaminating cells due to the significant variability in isolation methods and the lack of specific MSC markers. This review article focuses on recent developments in the MSC research field, with a special emphasis on the identification of novel surface markers for the in vivo localization and prospective isolation of murine and human MSCs. Furthermore, we discuss the physiological importance of MSC subtypes in vivo with specific reference to data supporting their contribution to HSC niche homeostasis. The isolation of MSCs using selective markers (combination of PDGFR α and Sca-1) is crucial to address the many unanswered questions pertaining to these cells and has the potential to enhance their therapeutic potential enormously.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Mabuchi Y,Houlihan DD,Akazawa C,Okano H,Matsuzaki Ydoi
10.1155/2013/507301subject
Has Abstractpub_date
2013-01-01 00:00:00pages
507301eissn
1687-966Xissn
1687-9678journal_volume
2013pub_type
杂志文章abstract::Endometriosis is an inflammatory disease with predominance of immunosuppressive M2 macrophages in the pelvic cavity that could be involved in the pathology through support and immune escape of ectopic lesions. Mesenchymal stromal cells (MSC) are found in ectopic lesions, and MSC from nonendometriosis sources are known...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3215962
更新日期:2017-01-01 00:00:00
abstract::The use of stem cells as a strategy for tissue repair and regeneration is one of the biomedical research areas that has attracted more interest in the past few years. Despite the classic belief that the central nervous system (CNS) was immutable, now it is well known that cell turnover occurs in the mature CNS. Postna...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/804717
更新日期:2012-01-01 00:00:00
abstract::Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, characterized by progressive cartilage degradation, subchondral bone remodeling, synovitis, and chronic pain. Due to the limited self-healing capacity in condylar cartilage, traditional clinical treatments have limited symptom-modifying and str...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/5979741
更新日期:2017-01-01 00:00:00
abstract:Background:Ly6Chigh monocytes are inflammatory cells that accumulate in an infarcted myocardium, and Ly6Clow monocytes are believed to be reparative and curb myocardial remodeling. NR4A1 is a novel target for modulating the inflammatory phenotype of monocytes during atherogenesis. Objectives:We aimed to investigate wh...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/2460158
更新日期:2020-01-13 00:00:00
abstract::Hematopoietic stem cell (HSC) belongs to multipotent adult somatic stem cells. A single HSC can reconstitute the entire blood system via self-renewal, differentiation into all lineages of blood cells, and replenishment of cells lost due to attrition or disease in a person's lifetime. Although all blood and immune cell...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2015/148064
更新日期:2015-01-01 00:00:00
abstract::Therapies based on transplantation of mesenchymal stromal cells (MSC) hold promise for the management of inflammatory disorders. In chronic Chagas disease cardiomyopathy (CCC), caused by chronic infection with Trypanosoma cruzi, the exacerbated immune response plays a critical pathophysiological role and can be modula...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3282656
更新日期:2017-01-01 00:00:00
abstract:Background:Cancer stem cell (CSC) promotes angiogenesis which plays an important role in tumor occurrence, growth, and metastasis. Accurately, quantifying the tumor vasculature can help understanding CSC characteristics and improve cancer diagnosis, therapy planning, and evaluation. The objective of this study is to pr...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/7813729
更新日期:2018-04-03 00:00:00
abstract::Exosomes derived from cancer cells can affect various functions of mesenchymal stem cells (MSCs) via conveying microRNAs (miRs). miR-21 and miR-146a have been demonstrated to regulate MSC proliferation and transformation. Interleukin-6 (IL-6) secreted from transformed MSCs in turn favors the survival of multiple myelo...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/9012152
更新日期:2017-01-01 00:00:00
abstract::Stem cells are a promising cell source for regenerative medicine. Stem cell differentiation must be regulated for applications in regenerative medicine. Stem cells are surrounded by extracellular matrix (ECM) in vivo. The ECM is composed of many types of proteins and glycosaminoglycans that assemble into a complex str...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/6397820
更新日期:2016-01-01 00:00:00
abstract:Objective:Mesenchymal stem cells (MSCs) are considered a promising therapy for wound healing. Here, we explored the role of c-Jun in diabetic wound healing using human umbilical cord-derived MSCs (hUC-MSCs). Methods:Freshly isolated hUC-MSCs were subjected to extensive in vitro subcultivation. The cell proliferative a...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/7430968
更新日期:2020-01-14 00:00:00
abstract::Following the discovery of pluripotent stem (PS) cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells, there has been a great hope that injured tissues can be repaired by transplantation of ES/iPS-derived various specific types of cells such as neural stem cells (NSCs). Although PS cells can be i...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2015/630693
更新日期:2015-01-01 00:00:00
abstract::Retrotransposons comprise a significant fraction of mammalian genome with unclear functions. Increasing evidence shows that they are not just remnants of ancient retroviruses but play important roles in multiple biological processes. Retrotransposons are epigenetically silenced in most somatic tissues and become react...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/6239245
更新日期:2018-07-15 00:00:00
abstract::Fifty-six aphaereses were performed in 23 pediatric patients with malignant hematological and solid tumors, following three different protocols for PBPC mobilization and distributed as follows: A: seventeen mobilized with 4 g/m(2) of cyclophosphamide (CFA) and 10 μg/kg/day of granulocyte colony stimulating factor (G-C...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/4078215
更新日期:2016-01-01 00:00:00
abstract::Background. The aim of the study was to assess the histological effects of autologous infusion of adipose-derived stem cells (ADSC) on a chronic vocal fold scar in a rabbit model as compared to an untreated scar as well as in injection of hyaluronic acid. Study Design. Animal experiment. Method. We used 74 New Zealand...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/9010279
更新日期:2016-01-01 00:00:00
abstract::Tendinopathy is a tendon disorder characterized by activity-related pain, local edema, focal tenderness to palpation, and decreased strength in the affected area. Tendinopathy is prevalent in both athletes and the general population, highlighting the need to elucidate the pathogenesis of this disorder. Current treatme...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/4076578
更新日期:2016-01-01 00:00:00
abstract::The purpose of this study is to determine if patients with chronic patellar tendinopathy will improve clinically after the inoculation of bone marrow mononuclear cells (BM-MNCs). Eight patients with chronic patellar tendinopathy were included. Patients averaged 24 years old (range 14-35). All patients were refractory ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/953510
更新日期:2012-01-01 00:00:00
abstract::The CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening. However, the diploid status of most mammalian cells restricts the application of CRISPR/C...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/2601746
更新日期:2017-01-01 00:00:00
abstract::The maternal part of the placenta, the decidua, consists of maternal immune cells, decidual stromal cells, and extravillous fetal trophoblasts. In a successful pregnancy, these cell compartments interact to provide an intricate balance between fetal tolerance and antimicrobial defense. These processes are still poorly...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/8010961
更新日期:2017-01-01 00:00:00
abstract::Oral mesenchymal stem/progenitor cells (MSCs) are renowned in the field of tissue engineering/regeneration for their multilineage differentiation potential and easy acquisition. These cells encompass the periodontal ligament stem/progenitor cells (PDLSCs), the dental pulp stem/progenitor cells (DPSCs), the stem/progen...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/1327405
更新日期:2020-02-25 00:00:00
abstract::Ion channels orchestrate directed flux of ions through membranes and are essential for a wide range of physiological processes including depolarization and repolarization of biomechanical activity of cells. Besides their electrophysiological functions in the heart, recent findings have demonstrated that ion channels a...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/462161
更新日期:2012-01-01 00:00:00
abstract::Wnt signaling plays crucial role in regulating melanocyte stem cells/melanocyte differentiation in the hair follicle. However, how the Wnt signaling is balanced to be overactivated to control follicular melanocytes behavior remains unknown. Here, by using immunofluorescence staining, we showed that secreted frizzled-r...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/2857478
更新日期:2017-01-01 00:00:00
abstract::Transcription factors are tools repetitively used by the embryo to generate a variety of lineages. Hence, their context of activation is an important determinant of their ability to specifically trigger certain cell fates, but not others. The context is also consequential when considering directing differentiation of ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/6939438
更新日期:2016-01-01 00:00:00
abstract:Purpose:To determine the effect of the isolation technique and location upon the phenotype of human corneal stroma-derived cells (CSCs). Methods:CSCs were isolated from the corneal stroma center and periphery using the explant or enzymatic digestion technique. The native tissue was stained for functional markers, whil...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/9275248
更新日期:2017-01-01 00:00:00
abstract::The application of human bone marrow mesenchymal stem cells (hBM-MSCs) in cell-based clinical therapies is hindered by the limited number of cells remaining after the initial isolation process and by cellular senescence following in vitro expansion. Understanding the process of in vitro senescence in hBM-MSCs would en...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/7670316
更新日期:2019-07-11 00:00:00
abstract::Pulmonary emphysema is a respiratory condition characterized by alveolar destruction that leads to airflow limitation and reduced lung function. Although with extensive research, the pathophysiology of emphysema is poorly understood and effective treatments are still missing. Evidence suggests that mesenchymal stem ce...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/9492038
更新日期:2018-03-14 00:00:00
abstract::Obesity is a complex, multifactorial disease that has been extensively researched in recent times. Obesity is characterized by excess deposition of adipose tissue in response to surplus energy. Despite the negative connotations of adipose tissue (AT), it serves as a critical endocrine organ. Adipose tissue is a source...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/3206807
更新日期:2016-01-01 00:00:00
abstract::Whether liver epithelial cells contribute to the development of hepatic scarring by undergoing epithelial-to-mesenchymal transition (EMT) is a controversial issue. Herein, we revisit the concept of EMT in cholangiopathies, a group of severe hepatic disorders primarily targeting the bile duct epithelial cell (cholangio...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/2953727
更新日期:2016-01-01 00:00:00
abstract::Two of the main problems of stem cell and regenerative medicine are the exit of pluripotency and differentiation to functional cells or tissues. The answer to these two problems holds great value in the clinical translation of stem cell as well as regenerative medicine research. Although piling researches have reveale...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8483035
更新日期:2020-02-03 00:00:00
abstract::We aim to investigate the effects of adipose-derived stem cells (ASCs) transplantation on irradiation-induced skeletal muscle fibrosis. Sixty-four rabbits were randomly divided into ASCs group and PBS group followed by irradiation at unilateral hip with a single dose of 80 Gy. Nonirradiated side with normal skeletal m...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/5638204
更新日期:2016-01-01 00:00:00
abstract::Allogeneic cell-based therapies using adipose tissue-derived stromal cells (ASCs) offer an off-the-shelf alternative to autologous therapy. An underlying assumption is that ASC can modulate the immune response of the recipient. However, in vitro models are required to explore and identify cell interactions and mechani...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8868909
更新日期:2020-10-26 00:00:00