Abstract：
:Here, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells. While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like cells can be isolated from adult mice, it remained unknown if the spontaneous conversion of SSCs to ESC-like cells fails at some age. Similarly, there have been differences in the literature about the duration of cultures during which ESC-like cells may appear. We demonstrate the possibility to derive ESC-like cells from SSC cultures until they reach adolescence or up to 7 weeks of age, but we point out the impossibility to derive these cells from older, mature adult mice. The inability of real adult SSCs to shift to a pluripotent state coincides with a decline in expression of the core pluripotency genes Oct4, Nanog, and Sox2 in SSCs with age. At the same time genes of the spermatogonial differentiation pathway increase. The generated ESC-like cells were similar to ESCs and express pluripotency markers. In vitro they differentiate into all three germ lineages; they form complex teratomas after transplantation in SCID mice and produce chimeric mice.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Azizi H,Conrad S,Hinz U,Asgari B,Nanus D,Peterziel H,Hajizadeh Moghaddam A,Baharvand H,Skutella Tdoi
10.1155/2016/8216312subject
Has Abstractpub_date
2016-01-01 00:00:00pages
8216312eissn
1687-966Xissn
1687-9678journal_volume
2016pub_type
杂志文章abstract：:The quality of life of patients with chronic wounds can be extremely poor and, therefore, over the past decades, great efforts have been made to develop efficient strategies to improve the healing process and the social impact associated with these conditions. Cell based therapy, as a modern tissue engineering strateg...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2015/195096
更新日期：2015-01-01 00:00:00
abstract：:The Wnt signalling pathway plays a crucial role in the development of the nervous system. This signalling cascade is initiated upon binding of the secreted Wnt ligand to a member of the family of frizzled receptors. In the present study, we analysed the presence of frizzled-4 in the enteric nervous system of human inf...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/9076823
更新日期：2016-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into cells from the mesenchymal lineage. The hypoimmunogenic characteristic of MSCs has encouraged studies using allogeneic MSCs for the treatment of autoimmune diseases and inflammatory conditions. Promising preclinical results and the saf...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2017/9824698
更新日期：2017-01-01 00:00:00
abstract：:Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility in women of reproductive age. Chronic inflammation is considered to be the cause of ovarian dysfunction. Increasing evidence in animal studies and in preliminary clinical trials has demonstrated that MSCs possess immunomodulatory effe...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/9782373
更新日期：2019-09-12 00:00:00
abstract：:Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, characterized by progressive cartilage degradation, subchondral bone remodeling, synovitis, and chronic pain. Due to the limited self-healing capacity in condylar cartilage, traditional clinical treatments have limited symptom-modifying and str...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2017/5979741
更新日期：2017-01-01 00:00:00
abstract：:Bone tissue regeneration is considered to be the optimal solution for bone loss. However, diabetic patients have a greater risk of poor bone healing or bone grafting failure than nondiabetics. The purpose of this study was to investigate the influence of the complexes of an adipose-derived stem cell sheet (ASC sheet) ...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/2506463
更新日期：2019-07-31 00:00:00
abstract：:The objective of this paper is to describe in detail the method of organotypic longitudinal spinal cord slice culture and the scientific basis for its potential utility. The technique is based on the interface method, which was described previously and thereafter was modified in our laboratory. The most important adva...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2015/471216
更新日期：2015-01-01 00:00:00
abstract：:The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of muscle tissue. These diso...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/5980465
更新日期：2019-04-24 00:00:00
abstract：:Podocyte death and regeneration are major topics in kidney research but remain controversial. Data obtained in humans demonstrate the existence of cells sited along Bowman's capsule that behave as podocyte progenitors in vitro and in in vivo mouse models of podocyte injury xenotrasplanted with this human-derived popul...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2015/678347
更新日期：2015-01-01 00:00:00
abstract：:Tendinopathy is a tendon disorder characterized by activity-related pain, local edema, focal tenderness to palpation, and decreased strength in the affected area. Tendinopathy is prevalent in both athletes and the general population, highlighting the need to elucidate the pathogenesis of this disorder. Current treatme...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/4076578
更新日期：2016-01-01 00:00:00
abstract：:Aging of population brings related social problems, such as muscle attenuation and regeneration barriers with increased aging. Muscle repair and regeneration depend on muscle stem cells (MuSCs). Obstructive sleep apnea (OSA) rises in the aging population. OSA leads to hypoxia and upper airway muscle injury. However, l...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/8412598
更新日期：2020-03-04 00:00:00
abstract：:Adipogenesis is the process through which preadipocytes differentiate into adipocytes. During this process, the preadipocytes cease to proliferate, begin to accumulate lipid droplets, and develop morphologic and biochemical characteristics of mature adipocytes. Mesenchymal stem cells (MSCs) are a type of adult stem ce...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2020/8892300
更新日期：2020-08-12 00:00:00
abstract：:Mesenchymal stem/stromal cells (MSCs) are known to be useful for treating local bone diseases. However, it is not known if MSCs are effective for treating systemic bone diseases, as the risk for mortality following intravenous MSC administration has hindered research progress. In this study, we compared the safety and...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/4214281
更新日期：2019-11-12 00:00:00
abstract：:Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripo...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2014/409021
更新日期：2014-01-01 00:00:00
abstract：:Aged tendon-derived stem/progenitor cells (TSPCs) lead to age-related tendon disorders and impair tendon healing. However, the underlying molecular mechanisms of TSPC aging remain largely unknown. Here, we investigated the role of connective tissue growth factor (CTGF) in TSPC aging. CTGF protein and mRNA levels were ...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/6257537
更新日期：2019-11-11 00:00:00
abstract：:A major challenge in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into damaged areas and strategies to promote this should be developed. The aim of this study was to evaluate the effect of peripheral blood derived mononuclear cell (PBMC) stimulation on mesenchymal stromal cells (MSC...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2015/323454
更新日期：2015-01-01 00:00:00
abstract：:We aim to investigate the effects of adipose-derived stem cells (ASCs) transplantation on irradiation-induced skeletal muscle fibrosis. Sixty-four rabbits were randomly divided into ASCs group and PBS group followed by irradiation at unilateral hip with a single dose of 80 Gy. Nonirradiated side with normal skeletal m...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/5638204
更新日期：2016-01-01 00:00:00
abstract：:Osteoporosis, femoral head necrosis, and congenital bone defects are orthopedic disorders characterized by reduced bone generation and insufficient bone mass. Bone regenerative therapy primarily relies on the bone marrow mesenchymal stem cells (BMSCs) and their ability to differentiate osteogenically. Icariin (ICA) is...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2019/5747298
更新日期：2019-04-04 00:00:00
abstract：:Mandibular bone defect reconstruction is an urgent challenge due to the requirements for daily eating and facial aesthetics. Three-dimensional- (3D-) printed titanium (Ti) scaffolds could provide patient-specific implants for bone defects. Appropriate load-bearing properties are also required during bone reconstructio...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2018/2074021
更新日期：2018-09-05 00:00:00
abstract：:Titanium (Ti) is widely used as a biomaterial for endosseous implants due to its relatively inert surface oxide layer that enables implanted devices the ability of assembling tissue reparative components that culminate in osseointegration. Topographic modifications in the form of micro- and nanoscaled structures signi...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/5214501
更新日期：2019-07-01 00:00:00
abstract：:The mesenchymal stem cells (MSCs) are known as highly plastic stem cells and can differentiate into specialized tissues such as adipose tissue, osseous tissue, muscle tissue, and nervous tissue. The differentiation of mesenchymal stem cells is very important in regenerative medicine. Their differentiation process is r...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2020/3763069
更新日期：2020-08-01 00:00:00
abstract：:Efficient ex vivo expansion of hematopoietic stem cells with a concomitant preservation of stemness and self-renewal potential is still an unresolved ambition. Increased numbers of methods approaching this issue using three-dimensional (3D) cultures were reported. Here, we describe a simplified 3D hanging drop model f...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/4148093
更新日期：2016-01-01 00:00:00
abstract：:Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are characterized by their unique capacity to stepwise differentiate towards any particular cell type in an adult organism. Pluripotent stem cells provide a beneficial platform to model hereditary diseases and even cancer development. While the inc...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2019/9301382
更新日期：2019-02-25 00:00:00
abstract：:Homoeostasis of bone marrow microenvironment depends on a precise balance between cell proliferation and death, which is supported by the cellular-extracellular matrix crosstalk. Multipotent mesenchymal stromal cells (MSC) are the key elements to provide the specialized bone marrow microenvironment by supporting, main...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2019/6854080
更新日期：2019-06-02 00:00:00
abstract：:Human mesenchymal stem/stromal cells (hMSCs) have generated great interest in regenerative medicine mainly due to their multidifferentiation potential and immunomodulatory role. Although hMSC can be obtained from different tissues, the number of available cells is always low for clinical applications, thus requiring i...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2017/6597815
更新日期：2017-01-01 00:00:00
abstract：:Two of the main problems of stem cell and regenerative medicine are the exit of pluripotency and differentiation to functional cells or tissues. The answer to these two problems holds great value in the clinical translation of stem cell as well as regenerative medicine research. Although piling researches have reveale...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/8483035
更新日期：2020-02-03 00:00:00
abstract：:Tumor hypoxia is associated with malignant biological phenotype including enhanced angiogenesis and metastasis. Hypoxia increases the expression of vascular endothelial cell growth factor (VEGF), which directly participates in angiogenesis by recruiting endothelial cells into hypoxic area and stimulating their prolife...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/6439864
更新日期：2016-01-01 00:00:00
abstract：Background:Aging is a major risk factor for cardiovascular disease. Cysteine protease cathepsin K (CatK) has been implicated in the process of angiogenesis, but the exact roles of individual CatK in vessel formation during aging are poorly understood. Methods and Results:To study the putative role of CatK in ischemia-...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/6938620
更新日期：2020-06-30 00:00:00
abstract：:Stem cell has immense potential in regenerative cellular therapy. Mesenchymal stem cells (MSCs) can become a potential attractive candidate for therapy due to its remarkable ability of self-renewal and differentiation into three lineages, i.e., ectoderm, mesoderm, and endoderm. Stem cell holds tremendous promises in t...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2019/7513404
更新日期：2019-07-24 00:00:00
abstract：:This study evaluated the efficacy of mesenchymal stem cells (MSCs) from bone marrow of a third-party donor for refractory aGVHD. We report the first experience using MSCs to treat refractory aGVHD in 33 pediatric patients undergoing allogeneic HSCT from Turkey. Totally, 68 doses of bone marrow derived MSCs were infuse...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/1641402
更新日期：2016-01-01 00:00:00
