Abstract：
:The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of muscle tissue. These disorders are associated with the depletion of the satellite cell pool or with the loss of satellite cell functionality. Recently, the establishment and maintenance of quiescence in satellite cells have been linked to their metabolic state. In this work, we aimed to modulate metabolism in order to preserve the satellite cell pool. We made use of metformin, a calorie restriction mimicking drug, to ask whether metformin has an effect on quiescence, proliferation, and differentiation of satellite cells. We report that satellite cells, when treated with metformin in vitro, ex vivo, or in vivo, delay activation, Pax7 downregulation, and terminal myogenic differentiation. We correlate the metformin-induced delay in satellite cell activation with the inhibition of the ribosome protein RPS6, one of the downstream effectors of the mTOR pathway. Moreover, in vivo administration of metformin induces a belated regeneration of cardiotoxin- (CTX-) damaged skeletal muscle. Interestingly, satellite cells treated with metformin immediately after isolation are smaller in size and exhibit reduced pyronin Y levels, which suggests that metformin-treated satellite cells are transcriptionally less active. Thus, our study suggests that metformin delays satellite cell activation and differentiation by favoring a quiescent, low metabolic state.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Pavlidou T,Marinkovic M,Rosina M,Fuoco C,Vumbaca S,Gargioli C,Castagnoli L,Cesareni Gdoi
10.1155/2019/5980465subject
Has Abstractpub_date
2019-04-24 00:00:00pages
5980465eissn
1687-966Xissn
1687-9678journal_volume
2019pub_type
杂志文章abstract：:Ion channels orchestrate directed flux of ions through membranes and are essential for a wide range of physiological processes including depolarization and repolarization of biomechanical activity of cells. Besides their electrophysiological functions in the heart, recent findings have demonstrated that ion channels a...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2012/462161
更新日期：2012-01-01 00:00:00
abstract：:The concerted action of ion channels and pumps establishing a resting membrane potential has been most thoroughly studied in the context of excitable cells, most notably neurons, but emerging evidences indicate that they are also involved in controlling proliferation and differentiation of nonexcitable somatic stem ce...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2012/816049
更新日期：2012-01-01 00:00:00
abstract：:Faced with the end stage of heart disease, the current treatments only slow worsening of heart failure. Stem cells have the potential of self-renewal and differentiation. It is expected to replace and repair damaged myocardium. But many clinical trials have shown that the stem cell therapy of heart failure is modest o...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.4061/2011/310928
更新日期：2011-01-01 00:00:00
abstract：:Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cel...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2013/250740
更新日期：2013-01-01 00:00:00
abstract：:The implantation of stem cells in vivo is the ideal approach for the restoration of normal life functions, such as replenishing the decreasing levels of affected dopaminergic (DA) neurons during neurodegenerative disease conditions. However, combining stem cells with biomaterial scaffolds provides a promising strategy...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2018/3410168
更新日期：2018-06-03 00:00:00
abstract：Aim:Few haematopoietic stem cells (HSCs) injected systemically for therapeutic purposes actually reach sites of injury as the vast majority become entrapped within pulmonary capillaries. One promising approach to maintain circulating HSC numbers would be to separate subpopulations with smaller size and/or greater defor...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/8540706
更新日期：2019-09-05 00:00:00
abstract：:The use of Mesenchymal Stromal Cells (MSCs) aiming to treat cancer has shown very contradictory results. In an attempt to clarify the contradictory results reported in the literature and the possible role of human fallopian tube Mesenchymal Stromal Cells (htMSCs) against breast cancer, the aim of this study was to eva...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2015/796215
更新日期：2015-01-01 00:00:00
abstract：:Human bone marrow mesenchymal stem cells (hBM-MSCs) are the best characterized multipotent adult stem cells. Their self-renewal capacity, multilineage differentiation potential, and immunomodulatory properties have indicated that they can be used in many clinical therapies. In a previous work we studied the DNA methyl...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/5656701
更新日期：2016-01-01 00:00:00
abstract：:Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently demonstrated that lipo...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/4373410
更新日期：2016-01-01 00:00:00
abstract：:Spinal cord injury (SCI) represents one of the most complicated and heterogeneous pathological processes of central nervous system (CNS) impairments, which is still beyond functional regeneration. Transplantation of mesenchymal stem cells (MSCs) has been shown to promote the repair of the injured spinal cord tissues i...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2017/5251313
更新日期：2017-01-01 00:00:00
abstract：:Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, characterized by progressive cartilage degradation, subchondral bone remodeling, synovitis, and chronic pain. Due to the limited self-healing capacity in condylar cartilage, traditional clinical treatments have limited symptom-modifying and str...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2017/5979741
更新日期：2017-01-01 00:00:00
abstract：:The application of human bone marrow mesenchymal stem cells (hBM-MSCs) in cell-based clinical therapies is hindered by the limited number of cells remaining after the initial isolation process and by cellular senescence following in vitro expansion. Understanding the process of in vitro senescence in hBM-MSCs would en...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/7670316
更新日期：2019-07-11 00:00:00
abstract：:The cornea is a clear structure, void of blood, and lymphatic vessels, functioning as our window to the world. Limbal epithelial stem cells, occupying the area between avascular cornea and vascularized conjunctiva, have been implicated in tissue border maintenance, preventing conjunctivalisation and propagation of blo...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2018/8620172
更新日期：2018-05-08 00:00:00
abstract：:The development of chemoresistance to cisplatin regimens causes a poor prognosis in patients with advanced NSCLC. The role of noncanonical Wnt signaling in the regulation of properties of lung cancer stem cells and chemoresistance was interrogated, by accessing capacities of cell proliferation, migration, invasion, an...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/1690896
更新日期：2016-01-01 00:00:00
abstract：:Retrotransposons comprise a significant fraction of mammalian genome with unclear functions. Increasing evidence shows that they are not just remnants of ancient retroviruses but play important roles in multiple biological processes. Retrotransposons are epigenetically silenced in most somatic tissues and become react...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2018/6239245
更新日期：2018-07-15 00:00:00
abstract：:Age-related macular degeneration (AMD) is a leading cause of legal blindness in individuals over 60 years of age, characterized by the dysfunction of retinal pigmented epithelium cells, specifically in the macular area. Despite several treatment options, AMD therapy remains difficult, especially for exudative AMD. Mul...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2012/946090
更新日期：2012-01-01 00:00:00
abstract：:Bone tissue regeneration is considered to be the optimal solution for bone loss. However, diabetic patients have a greater risk of poor bone healing or bone grafting failure than nondiabetics. The purpose of this study was to investigate the influence of the complexes of an adipose-derived stem cell sheet (ASC sheet) ...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2019/2506463
更新日期：2019-07-31 00:00:00
abstract：:Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder. Current targeted therapies designed to inhibit the tyrosine kinase activity of the BCR-ABL oncoprotein have made a significant breakthrough in the treatment of CML patients. However, CML remains a chronic disease that a patient must manage for lif...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2013/724360
更新日期：2013-01-01 00:00:00
abstract：:Infections of emerging and reemerging viruses (SARS-CoVs, influenza H1N1, etc.) largely and globally affect human health. Animal models often fail to reflect a physiological status because of species tropism of virus infection. Conventional cell lines are usually genetically and phenotypically different from primary c...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/2421689
更新日期：2020-07-09 00:00:00
abstract：Objective:Mesenchymal stem cells (MSCs) are considered a promising therapy for wound healing. Here, we explored the role of c-Jun in diabetic wound healing using human umbilical cord-derived MSCs (hUC-MSCs). Methods:Freshly isolated hUC-MSCs were subjected to extensive in vitro subcultivation. The cell proliferative a...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2020/7430968
更新日期：2020-01-14 00:00:00
abstract：:Cardiovascular diseases (CVDs), including atherosclerosis, stroke, and myocardial infarction, is a major cause of death worldwide. In aspects of cell therapy against CVD, it is generally accepted that endothelial progenitor cells (EPCs) are potent neovascular modulators in ischemic tissues. In response to ischemic inj...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/8340257
更新日期：2016-01-01 00:00:00
abstract：:Transcription factors are tools repetitively used by the embryo to generate a variety of lineages. Hence, their context of activation is an important determinant of their ability to specifically trigger certain cell fates, but not others. The context is also consequential when considering directing differentiation of ...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/6939438
更新日期：2016-01-01 00:00:00
abstract：:Objectives. Acute lung injury (ALI) is a common clinical critical disease. Stem cells transplantation is recognized as an effective way to repair injured lung tissues. The present study was designed to evaluate the effects of mesenchymal stem cells (MSCs) on repair of lung and its mechanism. Methods. MSCs carrying GFP...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2017/3648020
更新日期：2017-01-01 00:00:00
abstract：:Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fat pad (BFP) is a specialized adipose tissue that is easy to harvest and contains a rich blood supply, and its harvesting causes low complications for patients. This review focuse...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2017/8354640
更新日期：2017-01-01 00:00:00
abstract：:Cardiac progenitor cells (CPCs) have rapidly advanced to clinical trials, yet little is known regarding their interaction with the microenvironment. Signaling cues present in the microenvironment change with development and disease. This work aims to assess the influence of two distinct signaling moieties on CPCs: cyc...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/8364382
更新日期：2016-01-01 00:00:00
abstract：:Within the field of regenerative medicine, many have sought to use stem cells as a promising way to heal human tissue; however, in the past few years, exosomes (packaged vesicles released from cells) have shown more exciting promise. Specifically, stem cell-derived exosomes have demonstrated great ability to provide t...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/5802529
更新日期：2016-01-01 00:00:00
abstract：:The purpose of the present study was to test the potential of mouse bone marrow-derived mesenchymal stem cells (BD-MSCs) in improving tear production in a mouse model of Sjögren's syndrome dry eye and to investigate the underlying mechanisms involved. NOD mice (n = 20) were randomized to receive i.p. injection of ster...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2017/3134543
更新日期：2017-01-01 00:00:00
abstract：:Designing and providing a scaffold are very important for the cells in tissue engineering. Polybutylene succinate (PBS) has high potential as a scaffold for bone regeneration due to its capacity in cell proliferation and differentiation. Also, stem cells from 3rd molar tooth germs were favoured in this study due to th...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/8792191
更新日期：2016-01-01 00:00:00
abstract：:Damage to the nervous system can cause devastating diseases or musculoskeletal dysfunctions and transplantation of progenitor stem cells can be an excellent treatment option in this regard. Preclinical studies demonstrate that untreated stem cells, unlike stem cells activated to differentiate into neuronal lineage, do...
journal_title：Stem cells international
pub_type： 杂志文章
doi：10.1155/2016/1035374
更新日期：2016-01-01 00:00:00
abstract：:Tumor hypoxia is associated with malignant biological phenotype including enhanced angiogenesis and metastasis. Hypoxia increases the expression of vascular endothelial cell growth factor (VEGF), which directly participates in angiogenesis by recruiting endothelial cells into hypoxic area and stimulating their prolife...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/6439864
更新日期：2016-01-01 00:00:00
