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Designing Microfluidic Devices to Sort Haematopoietic Stem Cells Based on Their Mechanical Properties.

Abstract：

Aim:Few haematopoietic stem cells (HSCs) injected systemically for therapeutic purposes actually reach sites of injury as the vast majority become entrapped within pulmonary capillaries. One promising approach to maintain circulating HSC numbers would be to separate subpopulations with smaller size and/or greater deformability from a heterogeneous population. This study tested whether this could be achieved using label-free microfluidic devices. Methods:2 straight (A-B) and 3 spiral (C-E) devices were fabricated with different dimensions. Cell sorting was performed at different flow rates after which cell diameter and stiffness were determined using micromanipulation. Cells isolated using the most efficient device were tested intravitally for their ability to home to the mouse injured gut. Results:Only straight Device B at a high flow rate separated HSCs with different mechanical properties. Side outlets collected mostly deformable cells (nominal rupture stress/σR = 6.81 kPa; coefficient of variation/CV = 0.31) at a throughput of 2.3 × 105 cells/min. All spiral devices at high flow rates separated HSCs with different stiffness and size. Inner outlets collected mostly deformable cells in Devices C (σR = 25.06 kPa; CV = 0.26), D (σR = 22.21 kPa; CV = 0.41), and E (σR = 29.26 kPa; CV = 0.27) at throughputs of 2.3 × 105 cells/min, 1.5 × 105 cells/min, and 1.6 × 105 cells/min, respectively. Since Device C separated cells with higher efficiency and throughput, it was utilized to test the homing ability of separated cells in vivo. Significantly more deformable cells were observed trafficking through the injured gut-interestingly, increased retention was not observed. Conclusion:This study applied microfluidics to separate subpopulations from one stem cell type based on their intrinsic mechanical heterogeneity. Fluid dynamics within curved devices most effectively separated HSCs. Such devices may benefit cellular therapy.

journal_name

Stem Cells Int

journal_title

Stem cells international

authors

Du M,Kavanagh D,Zhang Z,Kalia N

doi

10.1155/2019/8540706

subject

Has Abstract

pub_date

2019-09-05 00:00:00

pages

8540706

eissn

1687-966X

issn

1687-9678

journal_volume

2019

pub_type

杂志文章

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Designing Microfluidic Devices to Sort Haematopoietic Stem Cells Based on Their Mechanical Properties.