Abstract:
:Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into cells from the mesenchymal lineage. The hypoimmunogenic characteristic of MSCs has encouraged studies using allogeneic MSCs for the treatment of autoimmune diseases and inflammatory conditions. Promising preclinical results and the safety of allogeneic MSC transplantation have created the possibility of "off-the-shelf" clinical application of allogeneic cells. This study has aimed to evaluate the survival of untreated and IFN-γ- and TNF-α-treated (preactivated) allogeneic MSCs transplanted under the kidney capsule of immunocompetent mice together with the role of preactivated MSCs after cotransplantation with allogeneic islets. The preactivation of MSCs upregulated the gene expression of anti-inflammatory molecules and also enhanced their immunomodulatory capacity in vitro. In vivo, allogeneic MSCs provoked an immunogenic response, with the infiltration of inflammatory cells at the transplant site and full graft rejection in both the untreated and preactivated groups. Allogeneic islets cotransplanted with preactivated MSCs prolonged graft survival for about 6 days, compared with islet alone. The present results corroborate the hypothesis that allogeneic MSCs are not immune-privileged and that after playing their therapeutic role they are rejected. Strategies that reduce allogeneic MSC immunogenicity can potentially prolong their in vivo persistence and improve the therapeutic effects.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Oliveira RL,Chagastelles PC,Sesterheim P,Pranke Pdoi
10.1155/2017/9824698subject
Has Abstractpub_date
2017-01-01 00:00:00pages
9824698eissn
1687-966Xissn
1687-9678journal_volume
2017pub_type
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journal_title:Stem cells international
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pub_type: 杂志文章
doi:10.1155/2017/5180579
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abstract::[This corrects the article DOI: 10.1155/2017/3978595.]. ...
journal_title:Stem cells international
pub_type: 已发布勘误
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journal_title:Stem cells international
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journal_title:Stem cells international
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