Abstract:
:Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are characterized by their unique capacity to stepwise differentiate towards any particular cell type in an adult organism. Pluripotent stem cells provide a beneficial platform to model hereditary diseases and even cancer development. While the incidence of pancreatic diseases such as diabetes and pancreatitis is increasing, the understanding of the underlying pathogenesis of particular diseases remains limited. Only a few recent publications have contributed to the characterization of human pancreatic development in the fetal stage. Hence, most knowledge of pancreatic specification is based on murine embryology. Optimizing and understanding current in vitro protocols for pancreatic differentiation of ESCs and iPSCs constitutes a prerequisite to generate functional pancreatic cells for better disease modeling and drug discovery. Moreover, human pancreatic organoids derived from pluripotent stem cells, organ-restricted stem cells, and tumor samples provide a powerful technology to model carcinogenesis and hereditary diseases independent of genetically engineered mouse models. Herein, we summarize recent advances in directed differentiation of pancreatic organoids comprising endocrine cell types. Beyond that, we illustrate up-and-coming applications for organoid-based platforms.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Hohwieler M,Müller M,Frappart PO,Heller Sdoi
10.1155/2019/9301382subject
Has Abstractpub_date
2019-02-25 00:00:00pages
9301382eissn
1687-966Xissn
1687-9678journal_volume
2019pub_type
杂志文章,评审abstract::Whether liver epithelial cells contribute to the development of hepatic scarring by undergoing epithelial-to-mesenchymal transition (EMT) is a controversial issue. Herein, we revisit the concept of EMT in cholangiopathies, a group of severe hepatic disorders primarily targeting the bile duct epithelial cell (cholangio...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/2953727
更新日期:2016-01-01 00:00:00
abstract::Substantial progress has been made in identifying the extracellular signalling pathways that regulate neural stem and precursor cell biology in the central nervous system (CNS). The bone morphogenetic proteins (BMPs), in particular BMP4, are key players regulating neuronal and glial cell development from neural precur...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/9260592
更新日期:2016-01-01 00:00:00
abstract::Here, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells. While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like cells can be isolated...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/8216312
更新日期:2016-01-01 00:00:00
abstract::The development of chemoresistance to cisplatin regimens causes a poor prognosis in patients with advanced NSCLC. The role of noncanonical Wnt signaling in the regulation of properties of lung cancer stem cells and chemoresistance was interrogated, by accessing capacities of cell proliferation, migration, invasion, an...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/1690896
更新日期:2016-01-01 00:00:00
abstract::Mesenchymal stem/stromal cells (MSCs) are known to be useful for treating local bone diseases. However, it is not known if MSCs are effective for treating systemic bone diseases, as the risk for mortality following intravenous MSC administration has hindered research progress. In this study, we compared the safety and...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/4214281
更新日期:2019-11-12 00:00:00
abstract::The migration of administered mesenchymal stem cells (MSCs) to sites of injury via the bloodstream has been demonstrated. However, the underlying mechanisms of umbilical cord MSC adhesion to endothelial cells during transendothelial migration are still unclear. In this study, our data showed that IL-1β induced LFA-1 e...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/7267142
更新日期:2019-12-27 00:00:00
abstract::Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of D...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/2157451
更新日期:2018-05-09 00:00:00
abstract::Current tissue engineering techniques are limited by inadequate vascularisation and perfusion of cell-scaffold constructs. Microstructural patterning through biomimetic vascular channels within a polymer scaffold might induce neovascularization, allowing fabrication of large engineered constructs. The network of vascu...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.4061/2011/547247
更新日期:2010-12-01 00:00:00
abstract:Background:Aging is a major risk factor for cardiovascular disease. Cysteine protease cathepsin K (CatK) has been implicated in the process of angiogenesis, but the exact roles of individual CatK in vessel formation during aging are poorly understood. Methods and Results:To study the putative role of CatK in ischemia-...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/6938620
更新日期:2020-06-30 00:00:00
abstract::The maternal part of the placenta, the decidua, consists of maternal immune cells, decidual stromal cells, and extravillous fetal trophoblasts. In a successful pregnancy, these cell compartments interact to provide an intricate balance between fetal tolerance and antimicrobial defense. These processes are still poorly...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/8010961
更新日期:2017-01-01 00:00:00
abstract::The lung is a radiosensitive organ, which imposes limits on the therapeutic dose in thoracic radiotherapy. Irradiated alveolar epithelial cells promote radiation-related pneumonitis and fibrosis. However, the role of lung stem cells (LSCs) in the development of radiation-induced lung injury is still unclear. In this s...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/6312053
更新日期:2020-09-29 00:00:00
abstract::This study evaluated the efficacy of mesenchymal stem cells (MSCs) from bone marrow of a third-party donor for refractory aGVHD. We report the first experience using MSCs to treat refractory aGVHD in 33 pediatric patients undergoing allogeneic HSCT from Turkey. Totally, 68 doses of bone marrow derived MSCs were infuse...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/1641402
更新日期:2016-01-01 00:00:00
abstract::This study is aimed at investigating whether human umbilical cord mesenchymal stem cell- (hucMSC-) derived exosomes (hucMSC-exosomes) have a protective effect on acute myocardial infarction (AMI). Exosomes were characterized under transmission electron microscopy and the particles of exosomes were further examined thr...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/761643
更新日期:2015-01-01 00:00:00
abstract::Background and Objectives. Culture expanded multipotential stromal cells (MSCs) have considerable potential for bone regeneration therapy but their wider use is constrained by the lack of simple and predictive assays of functional potency. Extended passaging leads to loss of multipotency but speed of decline depends o...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/6129596
更新日期:2017-01-01 00:00:00
abstract::Stem cell has immense potential in regenerative cellular therapy. Mesenchymal stem cells (MSCs) can become a potential attractive candidate for therapy due to its remarkable ability of self-renewal and differentiation into three lineages, i.e., ectoderm, mesoderm, and endoderm. Stem cell holds tremendous promises in t...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2019/7513404
更新日期:2019-07-24 00:00:00
abstract::Wnt signaling plays crucial role in regulating melanocyte stem cells/melanocyte differentiation in the hair follicle. However, how the Wnt signaling is balanced to be overactivated to control follicular melanocytes behavior remains unknown. Here, by using immunofluorescence staining, we showed that secreted frizzled-r...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/2857478
更新日期:2017-01-01 00:00:00
abstract::Fetal dermal mesenchymal stem cells (FDMSCs), isolated from fetal skin, are serving as a novel MSC candidate with great potential in regenerative medicine. More recently, the paracrine actions, especially MSC-derived exosomes, are being focused on the vital role in MSC-based cellular therapy. This study was to evaluat...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/2402916
更新日期:2019-06-10 00:00:00
abstract::Intestinal stem cells are located at the base of the crypts and are surrounded by a complex structure called niche. This environment is composed mainly of epithelial cells and stroma which provides signals that govern cell maintenance, proliferation, and differentiation. Understanding how the niche regulates stem cell...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/8387297
更新日期:2017-01-01 00:00:00
abstract::Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fat pad (BFP) is a specialized adipose tissue that is easy to harvest and contains a rich blood supply, and its harvesting causes low complications for patients. This review focuse...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/8354640
更新日期:2017-01-01 00:00:00
abstract::In cholestatic liver diseases, impaired bile excretion disrupts lipid homeostasis. We investigated changes of lipid metabolism, including mitochondrial β-oxidation, in a rat model of bile duct ligation (BDL) in which chorionic plate-derived mesenchymal stem cells (CP-MSCs) were transplanted. Serum cholesterol level, w...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/5180579
更新日期:2017-01-01 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most aggressive malignant brain tumor and a subpopulation of glioma stem-like cells (GSCs) is likely responsible for the invariable recurrence following maximum resection and chemoradiotherapy. As most GSCs that are located in the perivascular and perinecrotic niches should be remo...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/5387041
更新日期:2018-08-23 00:00:00
abstract::Therapies based on transplantation of mesenchymal stromal cells (MSC) hold promise for the management of inflammatory disorders. In chronic Chagas disease cardiomyopathy (CCC), caused by chronic infection with Trypanosoma cruzi, the exacerbated immune response plays a critical pathophysiological role and can be modula...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3282656
更新日期:2017-01-01 00:00:00
abstract::The purpose of the present study was to test the potential of mouse bone marrow-derived mesenchymal stem cells (BD-MSCs) in improving tear production in a mouse model of Sjögren's syndrome dry eye and to investigate the underlying mechanisms involved. NOD mice (n = 20) were randomized to receive i.p. injection of ster...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3134543
更新日期:2017-01-01 00:00:00
abstract::Delayed administration of kallikrein after cerebral infarction can improve neurological function. However, the appropriate kallkrein treatment time after ischemic stroke has not been illuminated. In this study, we compared the long-term outcome among three kallikrein therapeutic regimens starting at different time poi...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/1706982
更新日期:2018-04-05 00:00:00
abstract::During embryonic development pluripotency is progressively lost irreversibly by cell division, differentiation, migration and organ formation. Terminally differentiated cells do not generate other kinds of cells. Pluripotent stem cells are a great source of varying cell types that are used for tissue regeneration or r...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.4061/2011/619583
更新日期:2011-01-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder. Current targeted therapies designed to inhibit the tyrosine kinase activity of the BCR-ABL oncoprotein have made a significant breakthrough in the treatment of CML patients. However, CML remains a chronic disease that a patient must manage for lif...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/724360
更新日期:2013-01-01 00:00:00
abstract::Coronary revascularization remains the standard treatment for obstructive coronary artery disease and can be accomplished by either percutaneous coronary intervention (PCI) or coronary artery bypass graft surgery. Considerable advances have rendered PCI the most common form of revascularization and improved clinical o...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/8270498
更新日期:2017-01-01 00:00:00
abstract::Neuromuscular diseases are caused by functional defects of skeletal muscles, directly via muscle pathology or indirectly via disruption of the nervous system. Extensive studies have been performed to improve the outcomes of therapies; however, effective treatment strategies have not been fully established for any majo...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/6241681
更新日期:2018-04-11 00:00:00
abstract::Endometriosis is an inflammatory disease with predominance of immunosuppressive M2 macrophages in the pelvic cavity that could be involved in the pathology through support and immune escape of ectopic lesions. Mesenchymal stromal cells (MSC) are found in ectopic lesions, and MSC from nonendometriosis sources are known...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3215962
更新日期:2017-01-01 00:00:00
abstract::Titanium (Ti) is widely used as a biomaterial for endosseous implants due to its relatively inert surface oxide layer that enables implanted devices the ability of assembling tissue reparative components that culminate in osseointegration. Topographic modifications in the form of micro- and nanoscaled structures signi...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/5214501
更新日期:2019-07-01 00:00:00