Abstract:
:Current tissue engineering techniques are limited by inadequate vascularisation and perfusion of cell-scaffold constructs. Microstructural patterning through biomimetic vascular channels within a polymer scaffold might induce neovascularization, allowing fabrication of large engineered constructs. The network of vascular channels within a frontal-parietal defect in a patient, originating from the anterior branch of the middle meningeal artery, was modeled using computer-aided design (CAD) techniques and subsequently incorporated into polycaprolactone (PCL) scaffolds fabricated using fused deposition modeling (FDM). Bone marrow-derived mesenchymal stem cells (MSCs) were seeded onto the scaffolds and implanted into a rat model, with an arteriovenous bundle inserted at the proximal extent of the vascular network. After 3 weeks, scaffolds were elevated as a prefabricated composite tissue-polymer flap and transferred using microsurgical technique. Histological examination of explanted scaffolds revealed vascular ingrowth along patterned channels, with abundant capillary and connective tissue formation throughout experimental scaffolds, while control scaffolds showed only granulation tissue. All prefabricated constructs transferred as free flaps survived and were viable. We term this concept "vascular guidance," whereby neovascularization is guided through customized channels in a scaffold. Our technique might potentially allow fabrication of much larger tissue-engineered constructs than current technologies allow, as well as allowing tailored construct fabrication with a patient-specific vessel network based on CT scan data and CAD technology.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Muller D,Chim H,Bader A,Whiteman M,Schantz JTdoi
10.4061/2011/547247subject
Has Abstractpub_date
2010-12-01 00:00:00pages
547247eissn
1687-966Xissn
1687-9678journal_volume
2011pub_type
杂志文章abstract::Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripo...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/409021
更新日期:2014-01-01 00:00:00
abstract::The objective of this paper is to describe in detail the method of organotypic longitudinal spinal cord slice culture and the scientific basis for its potential utility. The technique is based on the interface method, which was described previously and thereafter was modified in our laboratory. The most important adva...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/471216
更新日期:2015-01-01 00:00:00
abstract::Stem cells are a promising cell source for regenerative medicine. Stem cell differentiation must be regulated for applications in regenerative medicine. Stem cells are surrounded by extracellular matrix (ECM) in vivo. The ECM is composed of many types of proteins and glycosaminoglycans that assemble into a complex str...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/6397820
更新日期:2016-01-01 00:00:00
abstract::Background. Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (n = ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/452102
更新日期:2012-01-01 00:00:00
abstract::Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in c...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/8303959
更新日期:2017-01-01 00:00:00
abstract::Hepatotoxicity is one of the most cited reasons for withdrawal of approved drugs from the market. The use of nonclinically relevant in vitro and in vivo testing systems contributes to the high attrition rates. Recent advances in differentiating human induced pluripotent stem cells (hiPSCs) into pure cultures of hepato...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/2475631
更新日期:2016-01-01 00:00:00
abstract:Background:Circulating angiogenic cells (CAC) have been identified as important regulators of vascular biology. However, there is still considerable debate about the genotype and function of CAC. Methods and Results:Data from publicly available gene expression data sets were used to analyse the transcriptome of in vit...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/9545261
更新日期:2019-05-02 00:00:00
abstract::Asherman's Syndrome (AS) is an uncommon, acquired, and refractory gynecological disorder. Current treatment was still limited, and stem cell-based therapy has been proposed as a novel strategy for management of AS. Here, we conducted a meta-analysis of self-controlled clinical trials to assess the effectiveness and sa...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8820538
更新日期:2020-12-19 00:00:00
abstract::Mesenchymal stem cells (MSCs) are currently defined as multipotent stromal cells that undergo sustained in vitro growth and can give rise to cells of multiple mesenchymal lineages, such as adipocytes, chondrocytes, and osteoblasts. The regenerative and immunosuppressive properties of MSCs have led to numerous clinical...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/507301
更新日期:2013-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) derived from induced pluripotent stem cells (iPSCs) are a promising cell source for the repair of skeletal disorders. Recently, neural crest cells (NCCs) were reported to be effective for inducing mesenchymal progenitors, which have potential to differentiate into osteochondral lineages. ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/1960965
更新日期:2017-01-01 00:00:00
abstract::Background. Mouse embryonic stem (ES) cells can be differentiated in vitro by aggregation and/or retinoic acid (RA) treatment. The principal differentiation lineage in vitro is extraembryonic primitive endoderm. Dab2, Laminin, GATA4, GATA5, and GATA6 are expressed in embryonic primitive endoderm and play critical role...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.4061/2010/602068
更新日期:2010-05-11 00:00:00
abstract::Neuromuscular diseases are caused by functional defects of skeletal muscles, directly via muscle pathology or indirectly via disruption of the nervous system. Extensive studies have been performed to improve the outcomes of therapies; however, effective treatment strategies have not been fully established for any majo...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/6241681
更新日期:2018-04-11 00:00:00
abstract::[This corrects the article DOI: 10.1155/2017/3978595.]. ...
journal_title:Stem cells international
pub_type: 已发布勘误
doi:10.1155/2017/9438310
更新日期:2017-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are one of the main stem cells that have been used for advanced therapies and regenerative medicine. To carry out the translational clinical application of MSCs, their manufacturing and administration in human must be controlled; therefore they should be considered as medicine: stem cell-...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/9783408
更新日期:2016-01-01 00:00:00
abstract::The cardiopulmonary system is made up of the heart and the lungs, with the core function of one complementing the other. The unimpeded and optimal cycling of blood between these two systems is pivotal to the overall function of the entire human body. Although the function of the cardiopulmonary system appears uncompli...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/3123961
更新日期:2018-04-29 00:00:00
abstract::Sepsis, newly defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection, is the most common cause of death in ICUs and one of the principal causes of death worldwide. Although substantial progress has been made in the understanding of fundamental mechanisms of sepsis, translat...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/7304121
更新日期:2017-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are a reliable source for cell-based regenerative medicine owing to their multipotency and biological functions. However, aging-induced systemic homeostasis disorders in vivo and cell culture passaging in vitro induce a functional decline of MSCs, switching MSCs to a senescent status with...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/8836258
更新日期:2020-09-10 00:00:00
abstract::Aged tendon-derived stem/progenitor cells (TSPCs) lead to age-related tendon disorders and impair tendon healing. However, the underlying molecular mechanisms of TSPC aging remain largely unknown. Here, we investigated the role of connective tissue growth factor (CTGF) in TSPC aging. CTGF protein and mRNA levels were ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/6257537
更新日期:2019-11-11 00:00:00
abstract::A major challenge in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into damaged areas and strategies to promote this should be developed. The aim of this study was to evaluate the effect of peripheral blood derived mononuclear cell (PBMC) stimulation on mesenchymal stromal cells (MSC...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/323454
更新日期:2015-01-01 00:00:00
abstract::Radiation is the most potent mode of cancer therapy; however, resistance to radiation therapy results in tumor relapse and subsequent fatality. The cancer stem cell (CSC), which has better DNA repair capability, has been shown to contribute to tumor resistance and is an important target for treatment. Signaling molecu...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/5785786
更新日期:2016-01-01 00:00:00
abstract::Known problems of the autologous chondrocyte implantation motivate the search for cellular alternatives. The aim of the study was to test the potential of synovium-derived stem cells (SMSC) to regenerate cartilage using a matrix-associated implantation. In an osteochondral defect model of the medial femoral condyle in...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/4142031
更新日期:2018-04-19 00:00:00
abstract::The purpose of this study is to explore why type 2 diabetes mellitus (T2DM) patients are susceptible to pulmonary tuberculosis through detection of serum Toll-like receptor 4 (TLR4), an important immune-related receptor, especially in terms of content and TLR4 gene polymorphism. Patients with T2DM complicated by pulmo...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/8160318
更新日期:2016-01-01 00:00:00
abstract::The mesenchymal stem cells (MSCs) are known as highly plastic stem cells and can differentiate into specialized tissues such as adipose tissue, osseous tissue, muscle tissue, and nervous tissue. The differentiation of mesenchymal stem cells is very important in regenerative medicine. Their differentiation process is r...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/3763069
更新日期:2020-08-01 00:00:00
abstract::The quality of life of patients with chronic wounds can be extremely poor and, therefore, over the past decades, great efforts have been made to develop efficient strategies to improve the healing process and the social impact associated with these conditions. Cell based therapy, as a modern tissue engineering strateg...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/195096
更新日期:2015-01-01 00:00:00
abstract::In situ detection of MSCs remains difficult and warrants additional methods to aid with their characterization in vivo. Two-photon confocal laser scanning microscopy (TPM) and second harmonic generation (SHG) could fill this gap. Both techniques enable the detection of cells and extracellular structures, based on intr...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/5457132
更新日期:2016-01-01 00:00:00
abstract::Recent studies indicate important roles for long noncoding RNAs (lncRNAs) as essential regulators of gene expression. However, the specific roles of lncRNAs in stenotic lesions of arteriovenous fistula (AVF) failure are still largely unknown. We first analyzed the expression profiles of lncRNAs in human stenosed and n...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/4252974
更新日期:2017-01-01 00:00:00
abstract::A growing body of evidence supports the argument that bone marrow-derived mesenchymal stem cells (MSCs) can differentiate into cardiomyocyte-like cells in an appropriate cellular environment, but the differentiation rate is low. A cocktail method was designed: we investigated the role of 5-azacytidine (5-aza), salvian...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/162024
更新日期:2014-01-01 00:00:00
abstract::Osteoporosis, femoral head necrosis, and congenital bone defects are orthopedic disorders characterized by reduced bone generation and insufficient bone mass. Bone regenerative therapy primarily relies on the bone marrow mesenchymal stem cells (BMSCs) and their ability to differentiate osteogenically. Icariin (ICA) is...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2019/5747298
更新日期:2019-04-04 00:00:00
abstract::Organoid is a cell organization grown in a three-dimensional (3D) culture system which represents all characteristics of its origin. However, this organ-like structure requires supporting matrix to maintain its characteristics and functions. Matrigel, derived from mouse sarcoma, has often been used as the supporting m...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/8472712
更新日期:2019-06-13 00:00:00
abstract::Intestinal stem cells are located at the base of the crypts and are surrounded by a complex structure called niche. This environment is composed mainly of epithelial cells and stroma which provides signals that govern cell maintenance, proliferation, and differentiation. Understanding how the niche regulates stem cell...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/8387297
更新日期:2017-01-01 00:00:00