Abstract:
:Background. Mouse embryonic stem (ES) cells can be differentiated in vitro by aggregation and/or retinoic acid (RA) treatment. The principal differentiation lineage in vitro is extraembryonic primitive endoderm. Dab2, Laminin, GATA4, GATA5, and GATA6 are expressed in embryonic primitive endoderm and play critical roles in its lineage commitment. Results. We found that in the absence of GATA4 or GATA5, RA-induced primitive endoderm differentiation of ES cells was reduced. GATA4 (-/-) ES cells express higher level of GATA5, GATA6, and hepatocyte nuclear factor 4 alpha marker of visceral endoderm lineage. GATA5 (-/-) ES cells express higher level of alpha fetoprotein marker of early liver development. GATA6 (-/-) ES cells express higher level of GATA5 as well as mesoderm and cardiomyocyte markers which are collagen III alpha-1 and tropomyosin1 alpha. Thus, deletion of GATA6 precluded endoderm differentiation but promoted mesoderm lineages. Conclusions. GATA4, GATA5, and GATA6 each convey a unique gene expression pattern and influences ES cell differentiation. We showed that ES cells can be directed to avoid differentiating into primitive endoderm and to adopt unique lineages in vitro by modulating GATA factors. The finding offers a potential approach to produce desirable cell types from ES cells, useful for regenerative cell therapy.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Capo-Chichi CD,Smedberg JL,Rula M,Nicolas E,Yeung AT,Adamo RF,Frolov A,Godwin AK,Xu XXdoi
10.4061/2010/602068subject
Has Abstractpub_date
2010-05-11 00:00:00pages
602068eissn
1687-966Xissn
1687-9678journal_volume
2010pub_type
杂志文章abstract::The application of human bone marrow mesenchymal stem cells (hBM-MSCs) in cell-based clinical therapies is hindered by the limited number of cells remaining after the initial isolation process and by cellular senescence following in vitro expansion. Understanding the process of in vitro senescence in hBM-MSCs would en...
journal_title:Stem cells international
pub_type: 杂志文章
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abstract::This study evaluated the efficacy of mesenchymal stem cells (MSCs) from bone marrow of a third-party donor for refractory aGVHD. We report the first experience using MSCs to treat refractory aGVHD in 33 pediatric patients undergoing allogeneic HSCT from Turkey. Totally, 68 doses of bone marrow derived MSCs were infuse...
journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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abstract::Due to their immunomodulatory and chemotactic properties, hMSC are being explored to treat immune-related diseases. For their use in human therapies, it is necessary to culture hMSC in xeno-free conditions. In this study, the impact that a xeno-free medium based on a human plasma derivate has on these properties was a...
journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
abstract::The clinical application of human bone marrow mesenchymal stem cells (hBM-MSCs) has generated a great deal of interest because of their potential use in regenerative medicine and tissue engineering. However, safety concerns over hBM-MSCs limit their clinical application. In this study, we observed that hBM-MSC-conditi...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/9501747
更新日期:2018-07-18 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/4373410
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/353097
更新日期:2013-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/1960965
更新日期:2017-01-01 00:00:00
abstract::Human liver stem cells (HLSCs) are a mesenchymal stromal cell-like population resident in the adult liver. Preclinical studies indicate that HLSCs could be a good candidate for cell therapy. The aim of the present study was to evaluate the immunogenicity and the immunomodulatory properties of HLSCs on T-lymphocytes, n...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/8468549
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/9783408
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/7414036
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/9564869
更新日期:2019-01-28 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/2402916
更新日期:2019-06-10 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/5251313
更新日期:2017-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/5670106
更新日期:2019-12-17 00:00:00
abstract::Human mesenchymal stem/stromal cells (hMSCs) have generated great interest in regenerative medicine mainly due to their multidifferentiation potential and immunomodulatory role. Although hMSC can be obtained from different tissues, the number of available cells is always low for clinical applications, thus requiring i...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/6597815
更新日期:2017-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/128732
更新日期:2012-01-01 00:00:00
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journal_title:Stem cells international
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doi:10.1155/2013/246134
更新日期:2013-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/3123961
更新日期:2018-04-29 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/6129596
更新日期:2017-01-01 00:00:00
abstract::Tendon healing is fraught with complications such as reruptures and adhesion formation due to the formation of scar tissue at the injury site as opposed to the regeneration of native tissue. Stem cells are an attractive option in developing cell-based therapies to improve tendon healing. However, several questions rem...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/291431
更新日期:2012-01-01 00:00:00
abstract::A major challenge in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into damaged areas and strategies to promote this should be developed. The aim of this study was to evaluate the effect of peripheral blood derived mononuclear cell (PBMC) stimulation on mesenchymal stromal cells (MSC...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/323454
更新日期:2015-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/9742034
更新日期:2016-01-01 00:00:00
abstract::The migration of administered mesenchymal stem cells (MSCs) to sites of injury via the bloodstream has been demonstrated. However, the underlying mechanisms of umbilical cord MSC adhesion to endothelial cells during transendothelial migration are still unclear. In this study, our data showed that IL-1β induced LFA-1 e...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/7267142
更新日期:2019-12-27 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/1035374
更新日期:2016-01-01 00:00:00