Abstract:
:Due to their immunomodulatory and chemotactic properties, hMSC are being explored to treat immune-related diseases. For their use in human therapies, it is necessary to culture hMSC in xeno-free conditions. In this study, the impact that a xeno-free medium based on a human plasma derivate has on these properties was analysed. Bone marrow-derived hMSC preserved their immunosuppressive and immunostimulatory properties, as observed with in vitro assays with hMSC cocultured with mixed leukocyte reactions or with mitogen-stimulated leukocytes. Moreover, hMSC expanded in xeno-free medium were recruited by macrophages in both migration and invasion assays, which indicates that the cells maintained their chemotactic properties. These data suggest that xeno-free expanded hMSC preserved their immunomodulatory and chemotactic properties, indicating that the described xeno-free medium composition is a potential candidate to culture and expand hMSC for human cell therapies.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Blázquez-Prunera A,Almeida CR,Barbosa MAdoi
10.1155/2017/2185351subject
Has Abstractpub_date
2017-01-01 00:00:00pages
2185351eissn
1687-966Xissn
1687-9678journal_volume
2017pub_type
杂志文章abstract::An intermediate filament protein, Nestin, is known as a neural stem/progenitor cell marker. It was shown to be required for the survival and self-renewal of neural stem cells according to the phenotypes of Nestin knockout mice. Nestin expression has also been reported in vascular endothelial cells, and we recently rep...
journal_title:Stem cells international
pub_type: 杂志文章
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journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章
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abstract::Within the field of regenerative medicine, many have sought to use stem cells as a promising way to heal human tissue; however, in the past few years, exosomes (packaged vesicles released from cells) have shown more exciting promise. Specifically, stem cell-derived exosomes have demonstrated great ability to provide t...
journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/2857478
更新日期:2017-01-01 00:00:00
abstract::A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3579283
更新日期:2017-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/9158016
更新日期:2019-06-11 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/5638204
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/5674921
更新日期:2020-06-17 00:00:00
abstract::Exosomes derived from cancer cells can affect various functions of mesenchymal stem cells (MSCs) via conveying microRNAs (miRs). miR-21 and miR-146a have been demonstrated to regulate MSC proliferation and transformation. Interleukin-6 (IL-6) secreted from transformed MSCs in turn favors the survival of multiple myelo...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/9012152
更新日期:2017-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/816049
更新日期:2012-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/8620172
更新日期:2018-05-08 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/507154
更新日期:2015-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/1690896
更新日期:2016-01-01 00:00:00
abstract::Oxidative stress leads to the degeneration of retinal pigment epithelial (RPE) and photoreceptor cells. We evaluated the potential of adipose-derived mesenchymal stem cells (ASCs) as a therapeutic tool by studying the migration capacity of ASCs in vitro and their protective effect against RPE cell death under oxidativ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/9682856
更新日期:2018-12-13 00:00:00
abstract::Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridi...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/250230
更新日期:2014-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/761643
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journal_title:Stem cells international
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1155/2012/921053
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journal_title:Stem cells international
pub_type: 杂志文章
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更新日期:2018-08-23 00:00:00
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journal_title:Stem cells international
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更新日期:2019-04-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/9545261
更新日期:2019-05-02 00:00:00
abstract:Background:Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a common type of liver failure with a high mortality. This study aimed at investigating the safety and efficacy of the combination treatment of plasma exchange (PE) and umbilical cord-derived mesenchymal stem cell (UC-MSCs) transplantatio...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/4130757
更新日期:2019-02-04 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/2152435
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/1286246
更新日期:2017-01-01 00:00:00
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更新日期:2012-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00