Current Progress and Challenges for Skeletal Muscle Differentiation from Human Pluripotent Stem Cells Using Transgene-Free Approaches.

Abstract:

:Neuromuscular diseases are caused by functional defects of skeletal muscles, directly via muscle pathology or indirectly via disruption of the nervous system. Extensive studies have been performed to improve the outcomes of therapies; however, effective treatment strategies have not been fully established for any major neuromuscular disease. Human pluripotent stem cells have a great capacity to differentiate into myogenic progenitors and skeletal myocytes for use in treating and modeling neuromuscular diseases. Recent advances have allowed the creation of patient-derived stem cells, which can be used as a unique platform for comprehensive study of disease mechanisms, in vitro drug screening, and potential new cell-based therapies. In the last decade, a number of methods have been developed to derive skeletal muscle cells from human pluripotent stem cells. By controlling the process of myogenesis using transcription factors and signaling molecules, human pluripotent stem cells can be directed to differentiate into cell types observed during muscle development. In this review, we highlight signaling pathways relevant to the formation of muscle tissue during embryonic development. We then summarize current methods to differentiate human pluripotent stem cells toward the myogenic lineage, specifically focusing on transgene-free approaches. Lastly, we discuss existing challenges for deriving skeletal myocytes and myogenic progenitors from human pluripotent stem cells.

journal_name

Stem Cells Int

journal_title

Stem cells international

authors

Jiwlawat N,Lynch E,Jeffrey J,Van Dyke JM,Suzuki M

doi

10.1155/2018/6241681

subject

Has Abstract

pub_date

2018-04-11 00:00:00

pages

6241681

eissn

1687-966X

issn

1687-9678

journal_volume

2018

pub_type

杂志文章,评审
  • Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors.

    abstract::Chemically induced liver progenitors (CLiPs) have promising applications in liver regenerative medicine. Three-dimensional (3D) structures generated from liver progenitor cells possess wide applications in cell transplantation, disease model, and drug testing. Here, we report on the spontaneous formation of 3D cystic ...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2019/3975689

    authors: Huang Y,Sakai Y,Hara T,Katsuda T,Ochiya T,Adachi T,Hidaka M,Gu WL,Eguchi S

    更新日期:2019-09-03 00:00:00

  • Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos.

    abstract::Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripo...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2014/409021

    authors: Guo J,Wu B,Li S,Bao S,Zhao L,Hu S,Sun W,Su J,Dai Y,Li X

    更新日期:2014-01-01 00:00:00

  • Ion flux dependent and independent functions of ion channels in the vertebrate heart: lessons learned from zebrafish.

    abstract::Ion channels orchestrate directed flux of ions through membranes and are essential for a wide range of physiological processes including depolarization and repolarization of biomechanical activity of cells. Besides their electrophysiological functions in the heart, recent findings have demonstrated that ion channels a...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2012/462161

    authors: Keßler M,Just S,Rottbauer W

    更新日期:2012-01-01 00:00:00

  • Treatment of chronic patellar tendinopathy with autologous bone marrow stem cells: a 5-year-followup.

    abstract::The purpose of this study is to determine if patients with chronic patellar tendinopathy will improve clinically after the inoculation of bone marrow mononuclear cells (BM-MNCs). Eight patients with chronic patellar tendinopathy were included. Patients averaged 24 years old (range 14-35). All patients were refractory ...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2012/953510

    authors: Pascual-Garrido C,Rolón A,Makino A

    更新日期:2012-01-01 00:00:00

  • Heart cells with regenerative potential from pediatric patients with end stage heart failure: a translatable method to enrich and propagate.

    abstract::Background. Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (n = ...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2012/452102

    authors: Steele A,Boucek RJ Jr,Jacobs JP,Steele P,Asante-Korang A,Chamizo W,Steele J,Chai PJ,Quintessenza JA

    更新日期:2012-01-01 00:00:00

  • Epigenetic Control of Haematopoietic Stem Cell Aging and Its Clinical Implications.

    abstract::Aging, chronic inflammation, and environmental insults play an important role in a number of disease processes through alterations of the epigenome. In this review we explore how age-related changes in the epigenetic landscape can affect heterogeneity within the haematopoietic stem cell (HSC) compartment and the deriv...

    journal_title:Stem cells international

    pub_type: 杂志文章,评审

    doi:10.1155/2016/5797521

    authors: Choudry FA,Frontini M

    更新日期:2016-01-01 00:00:00

  • Gene Profiles in the Early Stage of Neuronal Differentiation of Mouse Bone Marrow Stromal Cells Induced by Basic Fibroblast Growth Factor.

    abstract::A stably established population of mouse bone marrow stromal cells (BMSCs) with self-renewal and multilineage differentiation potential was expanded in vitro for more than 50 passages. These cells express high levels of mesenchymal stem cell markers and can be differentiated into adipogenic, chondrogenic, and osteogen...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2020/8857057

    authors: Yu L,Hong W,Yang H,Xia YY,Feng Z

    更新日期:2020-12-24 00:00:00

  • Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro.

    abstract::There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this wo...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2016/7414036

    authors: do Amaral RJ,da Silva NP,Haddad NF,Lopes LS,Ferreira FD,Filho RB,Cappelletti PA,de Mello W,Cordeiro-Spinetti E,Balduino A

    更新日期:2016-01-01 00:00:00

  • Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc.

    abstract::The clinical application of human bone marrow mesenchymal stem cells (hBM-MSCs) has generated a great deal of interest because of their potential use in regenerative medicine and tissue engineering. However, safety concerns over hBM-MSCs limit their clinical application. In this study, we observed that hBM-MSC-conditi...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2018/9501747

    authors: Chen B,Yu J,Wang Q,Zhao Y,Sun L,Xu C,Zhao X,Shen B,Wang M,Xu W,Zhu W

    更新日期:2018-07-18 00:00:00

  • Efficacy of topical mesenchymal stem cell therapy in the treatment of experimental dry eye syndrome model.

    abstract::Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridi...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2014/250230

    authors: Beyazyıldız E,Pınarlı FA,Beyazyıldız O,Hekimoğlu ER,Acar U,Demir MN,Albayrak A,Kaymaz F,Sobacı G,Delibaşı T

    更新日期:2014-01-01 00:00:00

  • In Vivo MRI Tracking of Mesenchymal Stromal Cells Labeled with Ultrasmall Paramagnetic Iron Oxide Particles after Intramyocardial Transplantation in Patients with Chronic Ischemic Heart Disease.

    abstract:Background:While regenerative stem cell therapy for ischemic heart disease has moved into phase 3 studies, little is still known about retention and migration of cell posttransplantation. In human studies, the ability to track transplanted cells has been limited to labeling with radioisotopes and tracking using nuclear...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2019/2754927

    authors: Mathiasen AB,Qayyum AA,Jørgensen E,Helqvist S,Ekblond A,Ng M,Bhakoo K,Kastrup J

    更新日期:2019-11-14 00:00:00

  • Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation.

    abstract::Left-right symmetry breaking is a complex developmental process and an important part of embryonic axis development. As of yet, the biophysical mechanism behind LR asymmetry establishment remains elusive for the overall asymmetry of embryos as well as for the organ-specific asymmetry. Here, we demonstrate that inheren...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2018/1848605

    authors: Worley KE,Chin AS,Wan LQ

    更新日期:2018-12-02 00:00:00

  • Mesenchymal Stem Cells Promoted Lung Wound Repair through Hox A9 during Endotoxemia-Induced Acute Lung Injury.

    abstract::Objectives. Acute lung injury (ALI) is a common clinical critical disease. Stem cells transplantation is recognized as an effective way to repair injured lung tissues. The present study was designed to evaluate the effects of mesenchymal stem cells (MSCs) on repair of lung and its mechanism. Methods. MSCs carrying GFP...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2017/3648020

    authors: Xin X,Yan L,Guangfa Z,Yan H,Keng L,Chunting W

    更新日期:2017-01-01 00:00:00

  • An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro.

    abstract::Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of D...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2018/2157451

    authors: Zhang L,Wu Y,Li X,Wei S,Xing Y,Lian Z,Han H

    更新日期:2018-05-09 00:00:00

  • Bone Morphogenetic Protein 4 Signalling in Neural Stem and Progenitor Cells during Development and after Injury.

    abstract::Substantial progress has been made in identifying the extracellular signalling pathways that regulate neural stem and precursor cell biology in the central nervous system (CNS). The bone morphogenetic proteins (BMPs), in particular BMP4, are key players regulating neuronal and glial cell development from neural precur...

    journal_title:Stem cells international

    pub_type: 杂志文章,评审

    doi:10.1155/2016/9260592

    authors: Cole AE,Murray SS,Xiao J

    更新日期:2016-01-01 00:00:00

  • Intrasplenic transplantation of bioencapsulated mesenchymal stem cells improves the recovery rates of 90% partial hepatectomized rats.

    abstract::Mesenchymal stem cells (MSCs) derived from bone marrow can secrete cytokines and growth factors and can transdifferentiate into liver cells. We transplanted polymeric membrane bioencapsulated MSCs into the spleens of 90% partial hepatectomized rats. This resulted in 91.6% recovery rates. This is compared to a recovery...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2012/697094

    authors: Liu ZC,Chang TM

    更新日期:2012-01-01 00:00:00

  • Ultraviolet Radiation-Induced Skin Aging: The Role of DNA Damage and Oxidative Stress in Epidermal Stem Cell Damage Mediated Skin Aging.

    abstract::Skin is the largest human organ. Skin continually reconstructs itself to ensure its viability, integrity, and ability to provide protection for the body. Some areas of skin are continuously exposed to a variety of environmental stressors that can inflict direct and indirect damage to skin cell DNA. Skin homeostasis is...

    journal_title:Stem cells international

    pub_type: 杂志文章,评审

    doi:10.1155/2016/7370642

    authors: Panich U,Sittithumcharee G,Rathviboon N,Jirawatnotai S

    更新日期:2016-01-01 00:00:00

  • Mesenchymal stem cell for prevention and management of intervertebral disc degeneration.

    abstract::Intervertebral disc degeneration (IVD) is a frequent pathological condition. Conservative management often fails, and patients with IVD degeneration may require surgical intervention. Several treatment strategies have been proposed, although only surgical discectomy and arthrodesis have been proved to be predictably e...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2012/921053

    authors: Longo UG,Papapietro N,Petrillo S,Franceschetti E,Maffulli N,Denaro V

    更新日期:2012-01-01 00:00:00

  • Enigma of Retrotransposon Biology in Mammalian Early Embryos and Embryonic Stem Cells.

    abstract::Retrotransposons comprise a significant fraction of mammalian genome with unclear functions. Increasing evidence shows that they are not just remnants of ancient retroviruses but play important roles in multiple biological processes. Retrotransposons are epigenetically silenced in most somatic tissues and become react...

    journal_title:Stem cells international

    pub_type: 杂志文章,评审

    doi:10.1155/2018/6239245

    authors: Yin Y,Zhou L,Yuan S

    更新日期:2018-07-15 00:00:00

  • Immunomodulatory effects of mesenchymal stromal cells revisited in the context of inflammatory cardiomyopathy.

    abstract::Myocarditis is a common inflammatory cardiomyopathy, associated with cardiomyocyte apoptosis, which can lead to chronic left ventricular dysfunction. Under conventional heart failure therapy, inflammatory cardiomyopathy typically has a progressive course, indicating a need for alternative therapeutic strategies to imp...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2013/353097

    authors: Miteva K,Van Linthout S,Volk HD,Tschöpe C

    更新日期:2013-01-01 00:00:00

  • Mesenchymal Stem Cells as Therapeutics Agents: Quality and Environmental Regulatory Aspects.

    abstract::Mesenchymal stem cells (MSCs) are one of the main stem cells that have been used for advanced therapies and regenerative medicine. To carry out the translational clinical application of MSCs, their manufacturing and administration in human must be controlled; therefore they should be considered as medicine: stem cell-...

    journal_title:Stem cells international

    pub_type: 杂志文章,评审

    doi:10.1155/2016/9783408

    authors: Galvez-Martin P,Sabata R,Verges J,Zugaza JL,Ruiz A,Clares B

    更新日期:2016-01-01 00:00:00

  • Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.

    abstract::Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from r...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2015/328957

    authors: Zhang J,Gao X,Zou H,Liu J,Zhang Z

    更新日期:2015-01-01 00:00:00

  • 3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis.

    abstract::Three-dimensional (3D) cell culture has been reported to increase the therapeutic potentials of mesenchymal stem cells (MSCs). However, the action mechanisms of 3D MSCs vary greatly and are far from being thoroughly investigated. In this study, we aimed to investigate the therapeutic effects of 3D spheroids of human a...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2016/4626073

    authors: Zhang X,Hu MG,Pan K,Li CH,Liu R

    更新日期:2016-01-01 00:00:00

  • Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants.

    abstract::The Wnt signalling pathway plays a crucial role in the development of the nervous system. This signalling cascade is initiated upon binding of the secreted Wnt ligand to a member of the family of frizzled receptors. In the present study, we analysed the presence of frizzled-4 in the enteric nervous system of human inf...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2016/9076823

    authors: Nothelfer K,Obermayr F,Belz N,Reinartz E,Bareiss PM,Bühring HJ,Beschorner R,Just L

    更新日期:2016-01-01 00:00:00

  • Efficient Promotion of Autophagy and Angiogenesis Using Mesenchymal Stem Cell Therapy Enhanced by the Low-Energy Shock Waves in the Treatment of Erectile Dysfunction.

    abstract:Background:Mesenchymal stem cell therapy (MSCT) and defocused low-energy shock wave therapy (ESWT) has been shown to ameliorate erectile dysfunction (ED). However, the interactions and effects of action between MSCT and ESWT remain poorly understood. In this study, we investigated the mechanisms of combination therapy ...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2018/1302672

    authors: Zhu GQ,Jeon SH,Bae WJ,Choi SW,Jeong HC,Kim KS,Kim SJ,Cho HJ,Ha US,Hong SH,Lee JY,Kwon EB,Kim SW

    更新日期:2018-08-29 00:00:00

  • Pivotal Cytoprotective Mediators and Promising Therapeutic Strategies for Endothelial Progenitor Cell-Based Cardiovascular Regeneration.

    abstract::Cardiovascular diseases (CVDs), including atherosclerosis, stroke, and myocardial infarction, is a major cause of death worldwide. In aspects of cell therapy against CVD, it is generally accepted that endothelial progenitor cells (EPCs) are potent neovascular modulators in ischemic tissues. In response to ischemic inj...

    journal_title:Stem cells international

    pub_type: 杂志文章,评审

    doi:10.1155/2016/8340257

    authors: Kim H,Kim S,Baek SH,Kwon SM

    更新日期:2016-01-01 00:00:00

  • Mesenchymal Stem Cells Derived and Cultured from Glioblastoma Multiforme Increase Tregs, Downregulate Th17, and Induce the Tolerogenic Phenotype of Monocyte-Derived Cells.

    abstract::Mesenchymal stem cells (MSCs) possess immunosuppressive properties and have been described in the tumor microenvironment of glioblastoma multiforme (GBM). This manuscript has two major topics-first, to describe isolated and cultured MSCs derived from GBM (GB-MSCs) and second, to examine their in vitro immunosuppressiv...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2019/6904638

    authors: Tumangelova-Yuzeir K,Naydenov E,Ivanova-Todorova E,Krasimirova E,Vasilev G,Nachev S,Kyurkchiev D

    更新日期:2019-05-02 00:00:00

  • Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression.

    abstract::Vitamin D (Vit D) by means of its biological active form, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3), has a protective effect on the skeleton by acting on calcium homeostasis and bone formation. Furthermore, Vit D has a direct effect on mesenchymal stem cells (MSCs) in stimulating their osteogenic differentiation. In thi...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2018/6958713

    authors: Posa F,Di Benedetto A,Cavalcanti-Adam EA,Colaianni G,Porro C,Trotta T,Brunetti G,Lo Muzio L,Grano M,Mori G

    更新日期:2018-02-28 00:00:00

  • Histone Arginine Methylation-Mediated Epigenetic Regulation of Discoidin Domain Receptor 2 Controls the Senescence of Human Bone Marrow Mesenchymal Stem Cells.

    abstract::The application of human bone marrow mesenchymal stem cells (hBM-MSCs) in cell-based clinical therapies is hindered by the limited number of cells remaining after the initial isolation process and by cellular senescence following in vitro expansion. Understanding the process of in vitro senescence in hBM-MSCs would en...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2019/7670316

    authors: Xu Z,Wu W,Shen F,Yu Y,Wang Y,Xiang C

    更新日期:2019-07-11 00:00:00

  • Delivery of Bone Marrow-Derived Mesenchymal Stem Cells Improves Tear Production in a Mouse Model of Sjögren's Syndrome.

    abstract::The purpose of the present study was to test the potential of mouse bone marrow-derived mesenchymal stem cells (BD-MSCs) in improving tear production in a mouse model of Sjögren's syndrome dry eye and to investigate the underlying mechanisms involved. NOD mice (n = 20) were randomized to receive i.p. injection of ster...

    journal_title:Stem cells international

    pub_type: 杂志文章

    doi:10.1155/2017/3134543

    authors: Aluri HS,Samizadeh M,Edman MC,Hawley DR,Armaos HL,Janga SR,Meng Z,Sendra VG,Hamrah P,Kublin CL,Hamm-Alvarez SF,Zoukhri D

    更新日期:2017-01-01 00:00:00