Abstract:
:Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of DF-1 feeder and basic culture medium supplemented with human basic fibroblast growth factor (hbFGF), mouse stem cell factor (mSCF), and human leukemia inhibitory factor (hLIF). During the long culture period, the cESCs showed typical ES cell morphology and expressed primitive stem cell markers with a relatively stable proliferation rate and high telomerase activity. These cells also exhibited the capability to differentiate into cardiac myocytes, smooth muscle cells, neural cells, osteoblast, and adipocyte in vitro. Chimera chickens were produced by cESCs cultured for 25 passages with this new culture system. The experiments showed that DF-1 was the optimal feeder and hbFGF was an important factor for maintaining the pluripotency of cESCs in vitro.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Zhang L,Wu Y,Li X,Wei S,Xing Y,Lian Z,Han Hdoi
10.1155/2018/2157451subject
Has Abstractpub_date
2018-05-09 00:00:00pages
2157451eissn
1687-966Xissn
1687-9678journal_volume
2018pub_type
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journal_title:Stem cells international
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