Abstract:
:Adipose-derived stem cells (ASCs) are a promising therapeutic alternative for tissue repair in various clinical applications. However, restrictive cell survival, differential tissue integration, and undirected cell differentiation after transplantation in a hostile microenvironment are complications that require refinement. Plasma rich in growth factors (PRGF) from platelet-rich plasma favors human and canine ASC survival, proliferation, and delaying human ASC senescence and autophagocytosis in comparison with serum-containing cultures. In addition, canine and human-derived ASCs efficiently differentiate into osteocytes, adipocytes, or chondrocytes in the presence of PRGF. PRGF treatment induces phosphorylation of AKT preventing ASC death induced by lethal concentrations of hydrogen peroxide. Indeed, AKT inhibition abolished the PRGF apoptosis prevention in ASC exposed to 100 μM of hydrogen peroxide. Here, we show that canine ASCs respond to PRGF stimulus similarly to the human cells regarding cell survival and differentiation postulating the use of dogs as a suitable translational model. Overall, PRGF would be employed as a serum substitute for mesenchymal stem cell amplification to improve cell differentiation and as a preconditioning agent to prevent oxidative cell death.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Mellado-López M,Griffeth RJ,Meseguer-Ripolles J,Cugat R,García M,Moreno-Manzano Vdoi
10.1155/2017/5946527subject
Has Abstractpub_date
2017-01-01 00:00:00pages
5946527eissn
1687-966Xissn
1687-9678journal_volume
2017pub_type
杂志文章abstract::In recent years several genes have linked amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) as a spectrum disease; however little is known about what triggers their onset. With the ability to generate patient specific stem cell lines from somatic cells, it is possible to model disease without the n...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/9279516
更新日期:2016-01-01 00:00:00
abstract::Bone tissue regeneration is considered to be the optimal solution for bone loss. However, diabetic patients have a greater risk of poor bone healing or bone grafting failure than nondiabetics. The purpose of this study was to investigate the influence of the complexes of an adipose-derived stem cell sheet (ASC sheet) ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/2506463
更新日期:2019-07-31 00:00:00
abstract::The implantation of stem cells in vivo is the ideal approach for the restoration of normal life functions, such as replenishing the decreasing levels of affected dopaminergic (DA) neurons during neurodegenerative disease conditions. However, combining stem cells with biomaterial scaffolds provides a promising strategy...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/3410168
更新日期:2018-06-03 00:00:00
abstract::Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are characterized by their unique capacity to stepwise differentiate towards any particular cell type in an adult organism. Pluripotent stem cells provide a beneficial platform to model hereditary diseases and even cancer development. While the inc...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2019/9301382
更新日期:2019-02-25 00:00:00
abstract::Homoeostasis of bone marrow microenvironment depends on a precise balance between cell proliferation and death, which is supported by the cellular-extracellular matrix crosstalk. Multipotent mesenchymal stromal cells (MSC) are the key elements to provide the specialized bone marrow microenvironment by supporting, main...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2019/6854080
更新日期:2019-06-02 00:00:00
abstract::Myocardial infarction is a leading cause of death among all cardiovascular diseases. Cell therapies using a cell population enriched with endothelial progenitor cells (EPCs), expanded CD133+ cells, have promise as a therapeutic option for the treatment of ischemic areas after infarction. Recently, secreted membrane ve...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/4802578
更新日期:2019-12-01 00:00:00
abstract:Background:While regenerative stem cell therapy for ischemic heart disease has moved into phase 3 studies, little is still known about retention and migration of cell posttransplantation. In human studies, the ability to track transplanted cells has been limited to labeling with radioisotopes and tracking using nuclear...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/2754927
更新日期:2019-11-14 00:00:00
abstract:Background:Aging is a major risk factor for cardiovascular disease. Cysteine protease cathepsin K (CatK) has been implicated in the process of angiogenesis, but the exact roles of individual CatK in vessel formation during aging are poorly understood. Methods and Results:To study the putative role of CatK in ischemia-...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/6938620
更新日期:2020-06-30 00:00:00
abstract::The objective of this paper is to describe in detail the method of organotypic longitudinal spinal cord slice culture and the scientific basis for its potential utility. The technique is based on the interface method, which was described previously and thereafter was modified in our laboratory. The most important adva...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/471216
更新日期:2015-01-01 00:00:00
abstract:Background:Ly6Chigh monocytes are inflammatory cells that accumulate in an infarcted myocardium, and Ly6Clow monocytes are believed to be reparative and curb myocardial remodeling. NR4A1 is a novel target for modulating the inflammatory phenotype of monocytes during atherogenesis. Objectives:We aimed to investigate wh...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/2460158
更新日期:2020-01-13 00:00:00
abstract::Following the discovery of pluripotent stem (PS) cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells, there has been a great hope that injured tissues can be repaired by transplantation of ES/iPS-derived various specific types of cells such as neural stem cells (NSCs). Although PS cells can be i...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2015/630693
更新日期:2015-01-01 00:00:00
abstract::There are remarkable similarities in the description of cancer stem cells (CSCs) and cancer cells with mesenchymal phenotype. Both cell types are highly tumorigenic, resistant against common anticancer treatment, and thought to cause metastatic growth. Moreover, cancer cells are able to switch between CSC and non-CSC ...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/4516454
更新日期:2018-10-10 00:00:00
abstract::MicroRNAs (miRNAs) play important roles in cell fate decisions. However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored. Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mES...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/1769298
更新日期:2017-01-01 00:00:00
abstract::Stem cells isolated from adipose tissue show great therapeutic potential in veterinary medicine, but some points such as the use of fresh or cultured cells and route of administration need better knowledge. This study aimed to evaluate the effect of autologous stromal vascular fraction (SVF, n = 4) or allogeneic cultu...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/391274
更新日期:2014-01-01 00:00:00
abstract:Objective:Parathyroid hormone (PTH) is considered to be essential during the tooth development. Stem cells from the apical papilla (SCAPs) are responsible for dentine formation. However, the interaction between PTH and SCAPs remains unclear. This study was aimed at investigating the effects of PTH on odonto/osteogenic ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/5128128
更新日期:2020-02-14 00:00:00
abstract::Previous studies have demonstrated that myocardin-related transcription factor A (MRTF-A) generates a link between the dynamics of the actin cytoskeleton and gene expression with its coregulator, serum response factor (SRF). MRTF-A has also been suggested as a regulator of stem cell differentiation. However, the role ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8853541
更新日期:2020-09-22 00:00:00
abstract:Background:Circulating angiogenic cells (CAC) have been identified as important regulators of vascular biology. However, there is still considerable debate about the genotype and function of CAC. Methods and Results:Data from publicly available gene expression data sets were used to analyse the transcriptome of in vit...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/9545261
更新日期:2019-05-02 00:00:00
abstract::Branching morphogenesis (BrM), an essential step for salivary gland development, requires epithelial-mesenchymal interactions. BrM is impaired when the surrounding mesenchyme is detached from the salivary epithelium during the pseudoglandular stage. It is believed that the salivary mesenchyme is indispensable for BrM,...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/502136
更新日期:2012-01-01 00:00:00
abstract::Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fat pad (BFP) is a specialized adipose tissue that is easy to harvest and contains a rich blood supply, and its harvesting causes low complications for patients. This review focuse...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/8354640
更新日期:2017-01-01 00:00:00
abstract::Two of the main problems of stem cell and regenerative medicine are the exit of pluripotency and differentiation to functional cells or tissues. The answer to these two problems holds great value in the clinical translation of stem cell as well as regenerative medicine research. Although piling researches have reveale...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8483035
更新日期:2020-02-03 00:00:00
abstract::Tumor microenvironment has been implicated in tumor development and progression. As a three-dimensional tumor microenvironment model, air liquid interface (ALI) organoid culture from oncogene transgenic mouse gastrointestinal tissues was recently produced. However, ALI organoid culture system from tissues of colorecta...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/7053872
更新日期:2016-01-01 00:00:00
abstract::The quality of life of patients with chronic wounds can be extremely poor and, therefore, over the past decades, great efforts have been made to develop efficient strategies to improve the healing process and the social impact associated with these conditions. Cell based therapy, as a modern tissue engineering strateg...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/195096
更新日期:2015-01-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility in women of reproductive age. Chronic inflammation is considered to be the cause of ovarian dysfunction. Increasing evidence in animal studies and in preliminary clinical trials has demonstrated that MSCs possess immunomodulatory effe...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/9782373
更新日期:2019-09-12 00:00:00
abstract::Neuromuscular diseases are caused by functional defects of skeletal muscles, directly via muscle pathology or indirectly via disruption of the nervous system. Extensive studies have been performed to improve the outcomes of therapies; however, effective treatment strategies have not been fully established for any majo...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/6241681
更新日期:2018-04-11 00:00:00
abstract::Placental structures, capable to persist in a genetically foreign organism, are a natural model of allogeneic engraftment carrying a number of distinctive properties. In this review, the main features of the placenta and its derivatives such as structure, cellular composition, immunological and endocrine aspects, and ...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/4837930
更新日期:2018-01-18 00:00:00
abstract::Radiation is the most potent mode of cancer therapy; however, resistance to radiation therapy results in tumor relapse and subsequent fatality. The cancer stem cell (CSC), which has better DNA repair capability, has been shown to contribute to tumor resistance and is an important target for treatment. Signaling molecu...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/5785786
更新日期:2016-01-01 00:00:00
abstract::Dental pulp stem cells (DPSCs) are ideal seed cells for the regeneration of dental tissues. However, DPSC senescence restricts its clinical applications. Metformin (Met), a common prescription drug for type 2 diabetes, is thought to influence the aging process. This study is aimed at determining the effects of metform...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2021/6616240
更新日期:2021-01-06 00:00:00
abstract::Wnt signalling has been implicated in the regulation of stem cell self-renewal and differentiation; however, the majority of in vitro studies are carried out using monolayer 2D culture techniques. Here, we used mesenchymal stromal cell (MSC) EGFP reporter lines responsive to Wnt pathway activation in a 3D spheroid cul...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/7132529
更新日期:2016-01-01 00:00:00
abstract::The concerted action of ion channels and pumps establishing a resting membrane potential has been most thoroughly studied in the context of excitable cells, most notably neurons, but emerging evidences indicate that they are also involved in controlling proliferation and differentiation of nonexcitable somatic stem ce...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/816049
更新日期:2012-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into cells from the mesenchymal lineage. The hypoimmunogenic characteristic of MSCs has encouraged studies using allogeneic MSCs for the treatment of autoimmune diseases and inflammatory conditions. Promising preclinical results and the saf...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/9824698
更新日期:2017-01-01 00:00:00