Abstract:
:Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth. In this study, we optimized the step-by-step procedure for ECM processing to develop a xeno-free ECM that supports the growth of undifferentiated hESCs. In addition, this newly developed xeno-free substrate can be stored at 4°C and is ready to use upon request, which serves as an easier way to amplify hESCs for clinical applications.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Zhu H,Yang J,Wei Y,Chen HHdoi
10.1155/2015/621057subject
Has Abstractpub_date
2015-01-01 00:00:00pages
621057eissn
1687-966Xissn
1687-9678journal_volume
2015pub_type
杂志文章abstract::Aging, which is accompanied by decreased organ function and increased disease incidence, limits human lifespan and has attracted investigators for thousands of years. In recent decades, with the rapid development of biology, scientists have shown that epigenetic modifications, especially DNA methylation, are key regul...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/1047896
更新日期:2020-07-08 00:00:00
abstract::Mesenchymal stem cells (MSCs) are multipotent stem cells that have a strong osteogenic differentiation capacity. However, the molecular mechanism underlying the osteogenic differentiation of MSCs remains largely unknown and thus hinders further development of MSC-based cell therapies for bone repair in the clinic. RSP...
journal_title:Stem cells international
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doi:10.1155/2020/7073805
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abstract::Tendinopathy is a tendon disorder characterized by activity-related pain, local edema, focal tenderness to palpation, and decreased strength in the affected area. Tendinopathy is prevalent in both athletes and the general population, highlighting the need to elucidate the pathogenesis of this disorder. Current treatme...
journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/6241681
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/7257628
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abstract::Myocarditis is a common inflammatory cardiomyopathy, associated with cardiomyocyte apoptosis, which can lead to chronic left ventricular dysfunction. Under conventional heart failure therapy, inflammatory cardiomyopathy typically has a progressive course, indicating a need for alternative therapeutic strategies to imp...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/353097
更新日期:2013-01-01 00:00:00
abstract::Ion channels orchestrate directed flux of ions through membranes and are essential for a wide range of physiological processes including depolarization and repolarization of biomechanical activity of cells. Besides their electrophysiological functions in the heart, recent findings have demonstrated that ion channels a...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/462161
更新日期:2012-01-01 00:00:00
abstract::Stem cell has immense potential in regenerative cellular therapy. Mesenchymal stem cells (MSCs) can become a potential attractive candidate for therapy due to its remarkable ability of self-renewal and differentiation into three lineages, i.e., ectoderm, mesoderm, and endoderm. Stem cell holds tremendous promises in t...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2019/7513404
更新日期:2019-07-24 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/5785786
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/5251313
更新日期:2017-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/5674921
更新日期:2020-06-17 00:00:00
abstract::Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic differentiation potenti...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/2152435
更新日期:2016-01-01 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most aggressive malignant brain tumor and a subpopulation of glioma stem-like cells (GSCs) is likely responsible for the invariable recurrence following maximum resection and chemoradiotherapy. As most GSCs that are located in the perivascular and perinecrotic niches should be remo...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/5387041
更新日期:2018-08-23 00:00:00
abstract:Background:Mesenchymal stem cell therapy (MSCT) and defocused low-energy shock wave therapy (ESWT) has been shown to ameliorate erectile dysfunction (ED). However, the interactions and effects of action between MSCT and ESWT remain poorly understood. In this study, we investigated the mechanisms of combination therapy ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/1302672
更新日期:2018-08-29 00:00:00
abstract::Recent studies indicate important roles for long noncoding RNAs (lncRNAs) as essential regulators of gene expression. However, the specific roles of lncRNAs in stenotic lesions of arteriovenous fistula (AVF) failure are still largely unknown. We first analyzed the expression profiles of lncRNAs in human stenosed and n...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/4252974
更新日期:2017-01-01 00:00:00
abstract::The migration of administered mesenchymal stem cells (MSCs) to sites of injury via the bloodstream has been demonstrated. However, the underlying mechanisms of umbilical cord MSC adhesion to endothelial cells during transendothelial migration are still unclear. In this study, our data showed that IL-1β induced LFA-1 e...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/7267142
更新日期:2019-12-27 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/1641402
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8853541
更新日期:2020-09-22 00:00:00
abstract:Objective:Parathyroid hormone (PTH) is considered to be essential during the tooth development. Stem cells from the apical papilla (SCAPs) are responsible for dentine formation. However, the interaction between PTH and SCAPs remains unclear. This study was aimed at investigating the effects of PTH on odonto/osteogenic ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/5128128
更新日期:2020-02-14 00:00:00
abstract::Fetal dermal mesenchymal stem cells (FDMSCs), isolated from fetal skin, are serving as a novel MSC candidate with great potential in regenerative medicine. More recently, the paracrine actions, especially MSC-derived exosomes, are being focused on the vital role in MSC-based cellular therapy. This study was to evaluat...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/2402916
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/946090
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.4061/2011/607569
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/3763069
更新日期:2020-08-01 00:00:00
abstract::Ischemic heart disease affects a majority of people, especially elderly patients. Recent studies have utilized autologous adult stem/progenitor cells as a treatment option to heal cardiac tissue after myocardial infarction. However, donor cells from aging patients are more likely to be in a senescent stage. Rejuvenati...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/9308301
更新日期:2018-03-29 00:00:00
abstract::In recent years several genes have linked amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) as a spectrum disease; however little is known about what triggers their onset. With the ability to generate patient specific stem cell lines from somatic cells, it is possible to model disease without the n...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/9279516
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/7370642
更新日期:2016-01-01 00:00:00
abstract::A mere 9 years have passed since the revolutionary report describing the derivation of induced pluripotent stem cells from human fibroblasts and the first in-patient translational use of cells obtained from these stem cells has already been achieved. From the perspectives of clinicians and researchers alike, the promi...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/4287158
更新日期:2016-01-01 00:00:00
abstract::The purpose of this study is to explore why type 2 diabetes mellitus (T2DM) patients are susceptible to pulmonary tuberculosis through detection of serum Toll-like receptor 4 (TLR4), an important immune-related receptor, especially in terms of content and TLR4 gene polymorphism. Patients with T2DM complicated by pulmo...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/8160318
更新日期:2016-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/921053
更新日期:2012-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/9501747
更新日期:2018-07-18 00:00:00