Abstract:
:Stem cell therapy with neural precursor cells (NPCs) has the potential to improve neuroregeneration after spinal cord injury (SCI). Unfortunately, survival and differentiation of transplanted NPCs in the injured spinal cord remains low. Growth factors have been successfully used to improve NPC transplantation in animal models, but their extensive application is associated with a relevant financial burden and might hinder translation of findings into the clinical practice. In our current study, we assessed the potential of a reduced number of growth factors in different combinations and concentrations to increase proliferation and differentiation of NPCs in vitro. After identifying a "cocktail" (EGF, bFGF, and PDGF-AA) that directed cell fate towards the oligodendroglial and neuronal lineage while reducing astrocytic differentiation, we translated our findings into an in vivo model of cervical clip contusion/compression SCI at the C6 level in immunosuppressed Wistar rats, combining NPC transplantation and intrathecal administration of the growth factors 10 days after injury. Eight weeks after SCI, we could observe surviving NPCs in the injured animals that had mostly differentiated into oligodendrocytes and oligodendrocytic precursors. Moreover, "Stride length" and "Average Speed" in the CatWalk gait analysis were significantly improved 8 weeks after SCI, representing beneficial effects on the functional recovery with NPC transplantation and the administration of the three growth factors. Nevertheless, no effects on the BBB scores could be observed over the course of the experiment and regeneration of descending tracts as well as posttraumatic myelination remained unchanged. However, reactive astrogliosis, as well as posttraumatic inflammation and apoptosis was significantly reduced after NPC transplantation and GF administration. Our data suggest that NPC transplantation is feasible with the use of only EGF, bFGF, and PDGF-AA as supporting growth factors.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Younsi A,Zheng G,Scherer M,Riemann L,Zhang H,Tail M,Hatami M,Skutella T,Unterberg A,Zweckberger Kdoi
10.1155/2020/5674921subject
Has Abstractpub_date
2020-06-17 00:00:00pages
5674921eissn
1687-966Xissn
1687-9678journal_volume
2020pub_type
杂志文章abstract::Cord blood transplantation (CBT) is an attractive alternative therapy in adult patients with advanced hematological malignancies in whom matched donors are unavailable. However, the risk of complications, especially infections, post-CBT increases the mortality rates in these patients. Although the incidence of acute a...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.4061/2011/607569
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abstract::A major challenge in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into damaged areas and strategies to promote this should be developed. The aim of this study was to evaluate the effect of peripheral blood derived mononuclear cell (PBMC) stimulation on mesenchymal stromal cells (MSC...
journal_title:Stem cells international
pub_type: 杂志文章
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abstract::Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility in women of reproductive age. Chronic inflammation is considered to be the cause of ovarian dysfunction. Increasing evidence in animal studies and in preliminary clinical trials has demonstrated that MSCs possess immunomodulatory effe...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/9782373
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/9492038
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abstract:Background:Ly6Chigh monocytes are inflammatory cells that accumulate in an infarcted myocardium, and Ly6Clow monocytes are believed to be reparative and curb myocardial remodeling. NR4A1 is a novel target for modulating the inflammatory phenotype of monocytes during atherogenesis. Objectives:We aimed to investigate wh...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/2460158
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abstract::Within the field of regenerative medicine, many have sought to use stem cells as a promising way to heal human tissue; however, in the past few years, exosomes (packaged vesicles released from cells) have shown more exciting promise. Specifically, stem cell-derived exosomes have demonstrated great ability to provide t...
journal_title:Stem cells international
pub_type: 杂志文章,评审
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abstract::Background. Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (n = ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/452102
更新日期:2012-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3282656
更新日期:2017-01-01 00:00:00
abstract::Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, characterized by progressive cartilage degradation, subchondral bone remodeling, synovitis, and chronic pain. Due to the limited self-healing capacity in condylar cartilage, traditional clinical treatments have limited symptom-modifying and str...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/5979741
更新日期:2017-01-01 00:00:00
abstract:Background:Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a common type of liver failure with a high mortality. This study aimed at investigating the safety and efficacy of the combination treatment of plasma exchange (PE) and umbilical cord-derived mesenchymal stem cell (UC-MSCs) transplantatio...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/4130757
更新日期:2019-02-04 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/761643
更新日期:2015-01-01 00:00:00
abstract::Allogeneic cell-based therapies using adipose tissue-derived stromal cells (ASCs) offer an off-the-shelf alternative to autologous therapy. An underlying assumption is that ASC can modulate the immune response of the recipient. However, in vitro models are required to explore and identify cell interactions and mechani...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8868909
更新日期:2020-10-26 00:00:00
abstract::Mesenchymal stem cells (MSCs) are a reliable source for cell-based regenerative medicine owing to their multipotency and biological functions. However, aging-induced systemic homeostasis disorders in vivo and cell culture passaging in vitro induce a functional decline of MSCs, switching MSCs to a senescent status with...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/8836258
更新日期:2020-09-10 00:00:00
abstract::Myocardial infarction is a leading cause of death among all cardiovascular diseases. Cell therapies using a cell population enriched with endothelial progenitor cells (EPCs), expanded CD133+ cells, have promise as a therapeutic option for the treatment of ischemic areas after infarction. Recently, secreted membrane ve...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/4802578
更新日期:2019-12-01 00:00:00
abstract::Human liver stem cells (HLSCs) are a mesenchymal stromal cell-like population resident in the adult liver. Preclinical studies indicate that HLSCs could be a good candidate for cell therapy. The aim of the present study was to evaluate the immunogenicity and the immunomodulatory properties of HLSCs on T-lymphocytes, n...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/8468549
更新日期:2016-01-01 00:00:00
abstract::Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in c...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/8303959
更新日期:2017-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/4076578
更新日期:2016-01-01 00:00:00
abstract::Whether liver epithelial cells contribute to the development of hepatic scarring by undergoing epithelial-to-mesenchymal transition (EMT) is a controversial issue. Herein, we revisit the concept of EMT in cholangiopathies, a group of severe hepatic disorders primarily targeting the bile duct epithelial cell (cholangio...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/2953727
更新日期:2016-01-01 00:00:00
abstract:Background:Mesenchymal stem cell therapy (MSCT) and defocused low-energy shock wave therapy (ESWT) has been shown to ameliorate erectile dysfunction (ED). However, the interactions and effects of action between MSCT and ESWT remain poorly understood. In this study, we investigated the mechanisms of combination therapy ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/1302672
更新日期:2018-08-29 00:00:00
abstract::Stem cells are a promising cell source for regenerative medicine. Stem cell differentiation must be regulated for applications in regenerative medicine. Stem cells are surrounded by extracellular matrix (ECM) in vivo. The ECM is composed of many types of proteins and glycosaminoglycans that assemble into a complex str...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/6397820
更新日期:2016-01-01 00:00:00
abstract::Periodontal ligament stem cells (PDLSCs) possess self-renewal, multilineage differentiation, and immunomodulatory properties. They play a crucial role in maintaining periodontal homeostasis and also participated in orthodontic tooth movement (OTM). Various studies have applied controlled mechanical stimulation to PDLS...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/6531216
更新日期:2018-09-17 00:00:00
abstract::The calcined tooth powder (CTP), a type of allogeneic biomimetic mineralized material, has been confirmed that can promote new bone formation when obtained at high temperature. The aim of this study was to investigate effects of the conditioned medium of calcined tooth powder (CTP-CM) on the osteogenic and odontogenic...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/4793518
更新日期:2019-03-19 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2019/4247168
更新日期:2019-01-08 00:00:00
abstract::Mesenchymal stem cells (MSCs) derived from bone marrow can secrete cytokines and growth factors and can transdifferentiate into liver cells. We transplanted polymeric membrane bioencapsulated MSCs into the spleens of 90% partial hepatectomized rats. This resulted in 91.6% recovery rates. This is compared to a recovery...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/697094
更新日期:2012-01-01 00:00:00
abstract::[This corrects the article DOI: 10.1155/2017/3978595.]. ...
journal_title:Stem cells international
pub_type: 已发布勘误
doi:10.1155/2017/9438310
更新日期:2017-01-01 00:00:00
abstract::Damage to the nervous system can cause devastating diseases or musculoskeletal dysfunctions and transplantation of progenitor stem cells can be an excellent treatment option in this regard. Preclinical studies demonstrate that untreated stem cells, unlike stem cells activated to differentiate into neuronal lineage, do...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/1035374
更新日期:2016-01-01 00:00:00
abstract::Osteoarthritis (OA) can be a debilitating degenerative disease and is the most common form of arthritic disease. There is a general consensus that current nonsurgical therapies are insufficient for younger OA sufferers who are not candidates for knee arthroplasties. Adipose-derived mesenchymal stem cells (MSCs) therap...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/421253
更新日期:2015-01-01 00:00:00
abstract::Faced with the end stage of heart disease, the current treatments only slow worsening of heart failure. Stem cells have the potential of self-renewal and differentiation. It is expected to replace and repair damaged myocardium. But many clinical trials have shown that the stem cell therapy of heart failure is modest o...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.4061/2011/310928
更新日期:2011-01-01 00:00:00
abstract:Materials and Methods:Ischemic brain injury was induced by dMCAO in Sprague-Dawley rats. The transplantation group received MSC infusion 1 h after dMCAO. Expression of IGF-1 in GFAP+ astrocytes, Iba-1+ microglia/macrophages, CD3+ lymphocytes, Ly6C+ monocytes/macrophages, and neutrophil elastase (NE)+ neutrophils was ex...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8853444
更新日期:2020-09-01 00:00:00
abstract::Chemically induced liver progenitors (CLiPs) have promising applications in liver regenerative medicine. Three-dimensional (3D) structures generated from liver progenitor cells possess wide applications in cell transplantation, disease model, and drug testing. Here, we report on the spontaneous formation of 3D cystic ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/3975689
更新日期:2019-09-03 00:00:00